Different packing motifs of isomeric (E)-N'-(halo-phenyl-methyl-idene)-N-methyl-2-(thio-phen-2-yl)acetohydrazides controlled by C-H⋯O inter-actions.

The crystal structures of three isomeric (E)-N'-(chloro-phenyl-methyl-idene)-N-methyl-2-(thio-phen-2-yl)acetohydrazides (C14H13ClN2OS) are described, with the Cl atom in ortho (I), meta (III) and para (IV) positions in the benzene ring. The ortho-bromo derivative (II) (C14H13BrN2OS), which is isostructural with its chloro congener (I), is also reported. Mol-ecules (I)-(III) have similar conformations, which approximate to l-shapes, as indicated by their N-C-C-Ct (t = thio-phene) torsion angles of -90.1 (3), -91.44 (18) and -90.7 (9)°, respectively. The conformation of (IV) is different, with an equivalent torsion angle of -170.75 (11)° corresponding to a more extended shape for the mol-ecule. The thio-phene ring in each structure features 'flip' rotational disorder. The packing for (I) and (II) features inversion dimers, linked by pairs of C-H⋯O inter-actions, which generate R22(14) loops. In the crystal of (III), [010] C(8) chains arise, with adjacent mol-ecules linked by pairs of C-H⋯O hydrogen bonds. The packing for (IV) features unusually short C-H⋯O inter-actions arising from an H atom attached to the benzene ring (H⋯O = 2.18 Å), which lead to C(9) [301] chains. Hirshfeld fingerprint percentage contact contributions are similar for the four title compounds.

Crystal structures and Hirshfeld surfaces of differently substituted (E)-N'-benzyl-idene-N-methyl-2-(thio-phen-2-yl)acetohydrazides.

The syntheses and crystal structures of (E)-N'-(3-cyano-benzyl-idene)-N-methyl-2-(thio-phen-2-yl)acetohydrazide, C15H13N3OS, (I), and (E)-N'-(4-meth-oxy-benzyl-idene)-N-methyl-2-(thio-phen-2-yl)acetohydrazide, C15H16N2O2S, (II), with different substituents in the meta and para position of the benzene ring are described. Compounds (I) and (II) both crystallize with two mol-ecules in the asymmetric unit, with generally similar conformations [r.m.s. overlay fits for (I) and (II) of 0.334 and 0.280 Å, respectively] that approximate to L-shapes. The thio-phene rings in (I) are well ordered, whereas those in (II) exhibit 'flip' rotational disorder [occupancies 0.662 (2) and 0.338 (2) for mol-ecule 1, and 0.549 (3) and 0.451 (3) for mol-ecule 2]. The packing for (I) features short C-H⋯O inter-actions arising from the C-H grouping adjacent to the cyanide group and C-H⋯Nc (c = cyanide) links arising from the methine groups to generate [110] double chains. Weak C-H⋯π inter-actions inter-link the chains into a three-dimensional network. The packing for (II) features numerous C-H⋯O and C-H⋯π inter-actions arising from different donor groups to generate a three-dimensional network. Hirshfeld fingerprint plots indicate significant differences in the percentage contact surfaces for (I) and (II).

Rendimento e acúmulo de nitrato em alface hidropônica sob proporções de nitrato e amônio / Yield and nitrate accumulation in lettuce hydroponic in different nitrate and ammonium ratios

O alto teor de nitrato em hortaliças é considerado um risco à saúde humana, principalmente naquelas cujo consumo é feito in natura, como é o caso da alface. Objetivando reduzir o teor de nitrato nas folhas de alface hidropônica através da adição de nitrogênio na forma de amônio na solução nutritiva, desenvolveu-se um experimento em casa-de-vegetação, pertencente ao Departamento de Fitotecnia da Universidade Federal de Santa Maria (UFSM/RS), durante o período de 13 de novembro a 30 de dezembro. O delineamento experimental foi inteiramente casualizado em esquema de parcelas subdivididas com quatro repetições, constando as parcelas de quatro proporções de NO3-:NH4+ na solução nutritiva (100:0-testemunha; 90:10; 80:20 e 70:30) e as subparcelas de três cultivares de alface (Regina, Mimosa e Verônica). Não houve interação entre cultivares e proporções de NO3-:NH4+ para nenhuma das variáveis avaliadas. A cultivar Regina apresentou maior número de folhas por planta e maior acúmulo de nitrato. A proporção 90:10 de NO3-:NH4+ proporcionou maior produção de massa fresca, maior número de folhas, reduzindo cerca de 20% o acúmulo de nitrato em relação ao obtido com a proporção 100:0. A proporção 80:20 manteve alto o rendimento e reduziu em 27% o acúmulo de nitrato, todavia a proporção 70:30 reduziu cerca de 36% o rendimento. Portanto, concentrações de amônio acima de 20% do nitrogênio [...](AU)

The high nitrate content in vegetables is considered a risk to the human health, mainly in those whose consumption is in natura, as in lettuce. With the objective of minimizing the nitrate content in hydroponic lettuce, a green-house experiment was carried out at the Crop Production Department, Federal University of Santa Maria (UFSM/RS), Brazil, from November 13th to December 30th. The experimental design was completely randomized in a split-plot with four replications, with the plots of four proportions of NO3-: NH4- in the nutrient solution (100:0-control, 90:10, 80:20 and 70:30) and subplots of three lettuce cultivars (Regina, Mimosa and Verônica). There was no interaction between lettuce cultivars and proportions of NO3-:NH4+for any of the variables evaluated. The cultivar Regina presented the largest number of leaves per plant and higher nitrate accumulation. The ratio 90:10 of NO3-:NH4+provided higher yield of fresh mass, more leaves, reducing about 20% of the nitrate accumulation in relation to that obtained from the proportion 100:0. The proportion 80:20 maintained the yield high and reduced about 27% of the nitrate accumulation, however the proportion 70:30 reduced about 36% of the yield. Therefore, ammonium concentrations above 20% of the total nitrogen in the nutrient solution reduce the yield and harm the visual quality of lettuce plants and the use of 10% of ammonium increase the yield and reduces significantly the nitrate content.(AU)

Rendimento e acúmulo de nitrato em alface hidropônica sob proporções de nitrato e amônio / Yield and nitrate accumulation in lettuce hydroponic in different nitrate and ammonium ratios

O alto teor de nitrato em hortaliças é considerado um risco à saúde humana, principalmente naquelas cujo consumo é feito in natura, como é o caso da alface. Objetivando reduzir o teor de nitrato nas folhas de alface hidropônica através da adição de nitrogênio na forma de amônio na solução nutritiva, desenvolveu-se um experimento em casa-de-vegetação, pertencente ao Departamento de Fitotecnia da Universidade Federal de Santa Maria (UFSM/RS), durante o período de 13 de novembro a 30 de dezembro. O delineamento experimental foi inteiramente casualizado em esquema de parcelas subdivididas com quatro repetições, constando as parcelas de quatro proporções de NO3-:NH4+ na solução nutritiva (100:0-testemunha; 90:10; 80:20 e 70:30) e as subparcelas de três cultivares de alface (Regina, Mimosa e Verônica). Não houve interação entre cultivares e proporções de NO3-:NH4+ para nenhuma das variáveis avaliadas. A cultivar Regina apresentou maior número de folhas por planta e maior acúmulo de nitrato. A proporção 90:10 de NO3-:NH4+ proporcionou maior produção de massa fresca, maior número de folhas, reduzindo cerca de 20% o acúmulo de nitrato em relação ao obtido com a proporção 100:0. A proporção 80:20 manteve alto o rendimento e reduziu em 27% o acúmulo de nitrato, todavia a proporção 70:30 reduziu cerca de 36% o rendimento. Portanto, concentrações de amônio acima de 20% do nitrogênio [...]

The high nitrate content in vegetables is considered a risk to the human health, mainly in those whose consumption is in natura, as in lettuce. With the objective of minimizing the nitrate content in hydroponic lettuce, a green-house experiment was carried out at the Crop Production Department, Federal University of Santa Maria (UFSM/RS), Brazil, from November 13th to December 30th. The experimental design was completely randomized in a split-plot with four replications, with the plots of four proportions of NO3-: NH4- in the nutrient solution (100:0-control, 90:10, 80:20 and 70:30) and subplots of three lettuce cultivars (Regina, Mimosa and Verônica). There was no interaction between lettuce cultivars and proportions of NO3-:NH4+for any of the variables evaluated. The cultivar Regina presented the largest number of leaves per plant and higher nitrate accumulation. The ratio 90:10 of NO3-:NH4+provided higher yield of fresh mass, more leaves, reducing about 20% of the nitrate accumulation in relation to that obtained from the proportion 100:0. The proportion 80:20 maintained the yield high and reduced about 27% of the nitrate accumulation, however the proportion 70:30 reduced about 36% of the yield. Therefore, ammonium concentrations above 20% of the total nitrogen in the nutrient solution reduce the yield and harm the visual quality of lettuce plants and the use of 10% of ammonium increase the yield and reduces significantly the nitrate content.

Different weak inter-actions in the crystals of three isomeric (E)-N-methyl-N'-(nitro-benzyl-idene)-2-(thio-phen-2-yl)acetohydrazides.

The crystal structures of three isomeric (E)-N-methyl-N'-(nitro-benzyl-idene)-2-(thio-phen-2-yl)acetohydrazides (formula C14H13N3O3S) are described, with the nitro group in ortho, meta and para positions in the benzene ring. In each crystal structure, mol-ecules are linked by various weak inter-actions (C-H⋯O and C-H⋯π bonds, and π-π stacking), leading to three-dimensional networks in each case, but with little similarity between them.

Lumbar myelography in a crab-eating raccoon (Procyon cancrivorus)

Background: The Crab-eating raccoon (Procyon cancrivorus) is a nocturnal mammal and lives in savannah, but loss of their natural habitat makes increase cases of traffic accidents on the roads. A growing number of wildlife are treated in veterinary hospitals, but we have some limitation about the correct interpretation of the diagnostic test, specially because, even the tests were not described. Myelography is a radiographic technique indicated for compressive lesions of the spinal cord that are not seen on plain radiographs. The present report aims to describe, for the first time, a lumbar myelogram performed in a paraplegic crab-eating raccoon after it suffer a traffic trauma. Case: A free living adult, 8-kg, female raccoon was presenting acute paraplegia and swelling in the region of the left humerus was referred at veterinary hospital. The patient was alert and aggressive, good body score and no signs of active bleeding. The animal temper did not allow approaching it; therefore, physical and neurological examinations were not feasible. The raccoon was tranquilized (1.6 mL of nilperidol® intramuscular), anesthetized (propofol, dose-effect intravenous) and tracheal intubation to provide oxygen and the patient was monitored continuously, followed by referred for radiology service. The simple radiograph showed a complete transverse fracture in the humerus and compression [...]

Lumbar myelography in a crab-eating raccoon (Procyon cancrivorus)

Background: The Crab-eating raccoon (Procyon cancrivorus) is a nocturnal mammal and lives in savannah, but loss of their natural habitat makes increase cases of traffic accidents on the roads. A growing number of wildlife are treated in veterinary hospitals, but we have some limitation about the correct interpretation of the diagnostic test, specially because, even the tests were not described. Myelography is a radiographic technique indicated for compressive lesions of the spinal cord that are not seen on plain radiographs. The present report aims to describe, for the first time, a lumbar myelogram performed in a paraplegic crab-eating raccoon after it suffer a traffic trauma. Case: A free living adult, 8-kg, female raccoon was presenting acute paraplegia and swelling in the region of the left humerus was referred at veterinary hospital. The patient was alert and aggressive, good body score and no signs of active bleeding. The animal temper did not allow approaching it; therefore, physical and neurological examinations were not feasible. The raccoon was tranquilized (1.6 mL of nilperidol® intramuscular), anesthetized (propofol, dose-effect intravenous) and tracheal intubation to provide oxygen and the patient was monitored continuously, followed by referred for radiology service. The simple radiograph showed a complete transverse fracture in the humerus and compression [...](AU)

Different hydrogen-bonded chains in the crystal structures of three alkyl N-[(E)-1-(2-benzyl-idene-1-methyl-hydrazin-yl)-3-hy-droxy-1-oxopropan-2-yl]carbamates.

The crystal structures of three methyl-ated hydrazine carbamate derivatives prepared by multi-step syntheses from l-serine are presented, namely benzyl N-{(E)-1-[2-(4-cyanobenzylidene)-1-methylhydrazinyl]-3-hydroxy-1-oxopro-pan-2-yl}carbamate, C20H20N4O4, tert-butyl N-{(E)-1-[2-(4-cyanobenzylidene)-1-methylhydrazinyl]-3-hydroxy-1-oxopropan-2-yl}carbamate, C17H22N4O4, and tert-butyl N-[(E)-1-(2-benzylidene-1-methylhydrazinyl)-3-hydroxy-1-oxopro-pan-2-yl]carbamate, C16H23N3O4. One of them shows that an unexpected racemization has occurred during the mild-condition methyl-ation reaction. In each crystal structure, the mol-ecules are linked into chains by O-H⋯O hydrogen bonds, but with significant differences between them.

Desafios para o desenvolvimento da tecnologia da criopreservação de sêmen felino / Challenges for the development of feline semen cryopreservation technology

O objetivo deste trabalho é levantar os pontos mais importantes que podem comprometer o resultado final do procedimento de criopreservação seminal em felinos.(AU)

The aim of this work is to raise the most important points that can affect the outcome of semen cryopreservation procedure in cats.(AU)

Dinâmica e estoque de carbono em floresta primária na região de Manaus/AM

This study analyzed data from three forest inventories conducted in the Experimental Forest, which belongs to Embrapa Western Amazon basin and is located at km 54 on BR-174, Manaus / AM. The aim was to study forest dynamics - the rates of recruitment, growth and mortality - in addition to carbon storage in an area of primary forest, without any intervention. The surveys were conducted in the years 2005, 2007 and 2010 in 15 permanent plots of 1 hectare each. There, all trees with diameter at breast height (DBH) above 10 cm were measured. We calculated the rates of recruitment and mortality, the periodic annual increments (PAIs) in terms of DBH, basal area and volume, fresh and dry biomass above the soil and total carbon storage in the vegetation. The recruitment rates were 2.0 percent and 1.8 percent, and mortality were 0.95 percent and 1.3 percent for the periods between 2005 and 2007 and 2007 and 2010, respectively. The volume was 345.62 m3 ha-1 for the year 2005, rising to 360.67 m3 ha-1 in 2010 with PAIs of 4.32 m3 ha-1 yr-1 between 2005 and 2007 and 1.31 m3 ha-1 yr-1 between 2007 and 2010. The total carbon storage increased from 173.63 t C ha-1 in 2005 to 181.01 t C ha-1 in 2010, confirming that the forest accumulated carbon in the period, acting as a sink.

Este trabalho analisou dados de três inventários florestais realizados na área da Floresta Experimental, pertencente à Embrapa Amazônia Ocidental e localizada no km 54 da BR-174, em Manaus/AM. O objetivo foi estudar a dinâmica da floresta - taxas de incremento, recrutamento e mortalidade - além do estoque de carbono, em uma área de floresta primária, sem qualquer tipo de intervenção. Os inventários foram realizados nos anos de 2005, 2007 e 2010, em 15 parcelas permanentes de 1 hectare cada, onde foram mensuradas todas as árvores com diâmetro a 1,3 m de altura do solo (DAP) superior a 10 cm. Foram calculadas as taxas de recrutamento e mortalidade, os incrementos periódicos anuais (IPAs) em termos de DAP, área basal e volume, as biomassas fresca e seca acima do nível do solo e total e o estoque de carbono da vegetação. As taxas de recrutamento foram de 2% e 1,8% e as de mortalidade foram de 0,95% e 1,3% para os períodos entre 2005 e 2007 e 2007 e 2010, respectivamente. O volume foi de 345,62 m3 ha-1 para o ano de 2005, aumentando para 360,67 m3 ha-1 em 2010, com IPAs de 4,32 m3 ha-1 ano-1 entre 2005 e 2007 e 1,31 m3 ha-1 ano-1 entre 2007 e 2010. O estoque de carbono total teve um acréscimo de 173,63 t C ha-1 em 2005 para 181,01 t C ha-1 em 2010, confirmando que a floresta acumulou carbono no período, atuando assim como sumidouro.

Desafios para o desenvolvimento da tecnologia da criopreservação de sêmen felino / Challenges for the development of feline semen cryopreservation technology

O objetivo deste trabalho é levantar os pontos mais importantes que podem comprometer o resultado final do procedimento de criopreservação seminal em felinos.

The aim of this work is to raise the most important points that can affect the outcome of semen cryopreservation procedure in cats.

Produção de cordeiros em pastejo contínuo de azevém anual submetido à adubação nitrogenada

It was evaluated the effect of nitrogen fertilization in ryegrass pasture (Lolium multiflorum Lam.) on the production of meat lamb under continuous grazing. Ryegrass pasture was established under no-tillage cropping in area of animal-crop integration system. The fertilization was held with 60kg of P2O5 ha-1 and 60kg of K²O ha-1. The treatments corresponded to the doses 0; 75; 150 e 225kg of N ha-1, in the urea form (45% de N) in single application, 35 days after seeding. The evaluation period was of 84 days. The forage was influenced quadratic ally by N rates, with minimum point at 182.75kg of N ha-1. However, the supply of leaf blades (LFM) was not influenced by doses of N (3.8kg LFM 100kg-1 live weight) or by the average daily weight gain, averaging 0.133 kg of LW an day. For stocking rate and live weight gain per area the doses of N increased 3.0 kg of LW-1 ha-1 and 1.1kg of LW ha-1, respectively for each kg of N applied. Nitrogen fertilization provides increases in animal productivity which results in increase kg of meat produced in this system.

Neste trabalho, avaliou-se o efeito da adubação nitrogenada do pasto de azevém (Lolium multiflorum Lam.) sobre a produção de cordeiros de corte mantidos em pastejo contínuo. O pasto foi implantado em plantio direto, em área de integração lavoura-pecuária. A adubação de fundação foi realizada com 60kg P2O5 ha-1 e 60kg K²O ha-1. Os tratamentos corresponderam às doses 0; 75; 150 e 225kg de N ha-1, na forma de ureia (45% de N), em cobertura, 35 dias após o plantio. O período de avaliação foi de 84 dias. A oferta de forragem foi influenciada de forma quadrática pelas doses de N, com menor disponibilidade para 182,75kg de N ha-1; no entanto, a oferta de lâminas foliares não foi influenciada pelas doses de N (3,8kg de massa de forragem de folhas/100kg de PV), nem o ganho de peso médio diário, com média 0,133kg de PV an-1 dia-1. Para carga animal e ganho de peso por área as doses de N proporcionaram aumento de 3,0kg de PV ha-1 e 1,1kg de PV ha-1, respectivamente por kg de N aplicado. A adubação nitrogenada proporcionou aumentos na carga animal, o que resulta no aumento de kg de carne produzidos nesse sistema.

Produção de cordeiros em pastejo contínuo de azevém anual submetido à adubação nitrogenada

It was evaluated the effect of nitrogen fertilization in ryegrass pasture (Lolium multiflorum Lam.) on the production of meat lamb under continuous grazing. Ryegrass pasture was established under no-tillage cropping in area of animal-crop integration system. The fertilization was held with 60kg of P2O5 ha-1 and 60kg of K²O ha-1. The treatments corresponded to the doses 0; 75; 150 e 225kg of N ha-1, in the urea form (45% de N) in single application, 35 days after seeding. The evaluation period was of 84 days. The forage was influenced quadratic ally by N rates, with minimum point at 182.75kg of N ha-1. However, the supply of leaf blades (LFM) was not influenced by doses of N (3.8kg LFM 100kg-1 live weight) or by the average daily weight gain, averaging 0.133 kg of LW an day. For stocking rate and live weight gain per area the doses of N increased 3.0 kg of LW-1 ha-1 and 1.1kg of LW ha-1, respectively for each kg of N applied. Nitrogen fertilization provides increases in animal productivity which results in increase kg of meat produced in this system.

Neste trabalho, avaliou-se o efeito da adubação nitrogenada do pasto de azevém (Lolium multiflorum Lam.) sobre a produção de cordeiros de corte mantidos em pastejo contínuo. O pasto foi implantado em plantio direto, em área de integração lavoura-pecuária. A adubação de fundação foi realizada com 60kg P2O5 ha-1 e 60kg K²O ha-1. Os tratamentos corresponderam às doses 0; 75; 150 e 225kg de N ha-1, na forma de ureia (45% de N), em cobertura, 35 dias após o plantio. O período de avaliação foi de 84 dias. A oferta de forragem foi influenciada de forma quadrática pelas doses de N, com menor disponibilidade para 182,75kg de N ha-1; no entanto, a oferta de lâminas foliares não foi influenciada pelas doses de N (3,8kg de massa de forragem de folhas/100kg de PV), nem o ganho de peso médio diário, com média 0,133kg de PV an-1 dia-1. Para carga animal e ganho de peso por área as doses de N proporcionaram aumento de 3,0kg de PV ha-1 e 1,1kg de PV ha-1, respectivamente por kg de N aplicado. A adubação nitrogenada proporcionou aumentos na carga animal, o que resulta no aumento de kg de carne produzidos nesse sistema.

Produção de cordeiros em pastejo contínuo de azevém anual submetido à adubação nitrogenada

It was evaluated the effect of nitrogen fertilization in ryegrass pasture (Lolium multiflorum Lam.) on the production of meat lamb under continuous grazing. Ryegrass pasture was established under no-tillage cropping in area of animal-crop integration system. The fertilization was held with 60kg of P2O5 ha-1 and 60kg of K²O ha-1. The treatments corresponded to the doses 0; 75; 150 e 225kg of N ha-1, in the urea form (45% de N) in single application, 35 days after seeding. The evaluation period was of 84 days. The forage was influenced quadratic ally by N rates, with minimum point at 182.75kg of N ha-1. However, the supply of leaf blades (LFM) was not influenced by doses of N (3.8kg LFM 100kg-1 live weight) or by the average daily weight gain, averaging 0.133 kg of LW an day. For stocking rate and live weight gain per area the doses of N increased 3.0 kg of LW-1 ha-1 and 1.1kg of LW ha-1, respectively for each kg of N applied. Nitrogen fertilization provides increases in animal productivity which results in increase kg of meat produced in this system.

Neste trabalho, avaliou-se o efeito da adubação nitrogenada do pasto de azevém (Lolium multiflorum Lam.) sobre a produção de cordeiros de corte mantidos em pastejo contínuo. O pasto foi implantado em plantio direto, em área de integração lavoura-pecuária. A adubação de fundação foi realizada com 60kg P2O5 ha-1 e 60kg K²O ha-1. Os tratamentos corresponderam às doses 0; 75; 150 e 225kg de N ha-1, na forma de ureia (45% de N), em cobertura, 35 dias após o plantio. O período de avaliação foi de 84 dias. A oferta de forragem foi influenciada de forma quadrática pelas doses de N, com menor disponibilidade para 182,75kg de N ha-1; no entanto, a oferta de lâminas foliares não foi influenciada pelas doses de N (3,8kg de massa de forragem de folhas/100kg de PV), nem o ganho de peso médio diário, com média 0,133kg de PV an-1 dia-1. Para carga animal e ganho de peso por área as doses de N proporcionaram aumento de 3,0kg de PV ha-1 e 1,1kg de PV ha-1, respectivamente por kg de N aplicado. A adubação nitrogenada proporcionou aumentos na carga animal, o que resulta no aumento de kg de carne produzidos nesse sistema.

Avaliação da concentração de arsênio, cádmio e chumbo em amostras de atum enlatadas, comercializadas na cidade do Rio de Janeiro, RJ / Determination of arsenic, cadmium and lead in samples of canned tuna commercialized in Rio de Janeiro, RJ

Atualmente, a população brasileira tem sido muito incentivada a consumir peixes ou outros frutos do mar, por serem alimentos que trazem benefícios importantes para a saúde humana, principalmente à dos adultos. Entretanto, em contrapartida aos benefícios, estes alimentos estão mais sujeitos a contaminação por substâncias químicas que são, altamente, prejudiciais à saúde. Dentre essas substâncias incluem-se o arsênio, que tem sua atividade carcinogênica já comprovada, o cádmio e o chumbo, que afetam o sistema nervoso central. O presente trabalho tem como objetivo determinar as concentrações de As, Cd e Pb, em amostras de atum enlatado e, simultaneamente, avaliar as variações que ocorrem, nestas concentrações, em função dos diferentes tipos de produtos disponíveis no mercado nacional.(...)(AU)

There are little data and advisories for arsenic, cadmium and lead in wild fish, but far fewer for commercial fish that compose the large majority of the fish most people eat. Until recently, relatively little attention has been devoted to examining As, Cd, Pb in canned tuna, despite its great importance in Brazilian diets, as it is considered to bring many benefits for the health. However, this kind of food is highly subjected to contamination by extremely toxic elements. The main inorganic elements that may contaminate this food are arsenic, cadmium and lead, which have been considered as some of the most toxicants elements as well as harmful to people' s health. The objective of this work is to study concentrations of As, Cd, and Pb in canned tuna and to study parameters conditions that can alter these concentrations. Graphite fumace atomic absorption spectrometry (GF AAS) was the technique chosen by the inorganic contamination laboratory of Instituto Nacional de Controle de Qualidade em Saúde (INCQS/ FIOCRUZ) for detection and quantification of arsenic, cadmium and lead in canned tuna. The obtained results for arsenic is 0,1 - 1,0 mg Kg -I, and the concentration for cadmium and lead is low of the limit of detection, with the exception of tree sample which obtained results for lead between 0,1 e 0,2 mg Kg -I. The result for repeatability and reproducibility (precision} for As, Cd and Pb is less than 15%, and recuperation ranging from 85 to 104%. Ali samples presented results lower than the maximum limit values established by the legal Brazilian regulations. (AU)

Abordagem do tabagismo na gestação: [revisão] / Approach of smoking in pregnancy: [review]

Objetivos: discutir os efeitos do cigarro e avaliar alternativas para estimular o abandono do tabagismo durante a gestação. Fonte de dados: revisão da literatura através das bases de dados Medline e Scielo. Síntese dos dados: o tabagismo causa sérios prejuízos à saúde humana e encontra-se associado ao desenvolvimento de múltiplas doenças. Seus efeitos na gestação já são devidamente conhecidos e relatados, tais como o crescimento intrauterino restrito e maior risco de trabalho de parto prematuro, entre outras alterações. A gestação torna-se um momento propício para a interrupção desta dependência.Conclusões: o cigarro tem inúmeros efeitos negativos sobre o organismo humano e esses efeitos podem ser ainda piores quando associados à gestação. A consulta médica do ginecologista e obstetra deve incluir abordagem do tabagismo, bem como os esclarecimentos e orientações sobre os malefícios do fumo. O papel educativo do médico e da equipede saúde é fundamental para o controle do tabagismo.

Aims: To discuss the effects of the tobacco and to evaluate alternatives to stimulate quitting smoking during pregnancy. Source of data: Literature review, MEDLINE and SCIELO. Summary of the findings: The smoking causes serious damages to the human health and it is associated with the development of multiple diseases. Their effects in the gestation are already properly recognized and related to restricted intra-uterine growth of the fetus, larger risk of premature labor among other alterations. The gestation becomes the bestmoment for the interruption of smoking dependence. Conclusions: Tobacco has many negative effects on the human body and these effects may be worse when associated with pregnancy. The medical consultation of the obstetrician and gynecologist's approach should include approach to smoking addiction as well as the clarification and guidance on the harmful effects of smoking should be made at thistime. The educational role of the physician and the health care team is essential for the control of smoking because it is the best and cheapest way to prevent, treat and cure various diseases.

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for approximately 0.13 percent of the venom dry weight and has a molecular mass of 32 kDa as determined by SDS-PAGE, and of 27 kDa as determined by MALDI-TOF mass spectrometry. Its partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this species. The N-terminal sequence of the enzyme is VIGGDECNINEHRSLVVLFXSSGFL CAGTLVQDEWVLTAANCDSKNFQ. The enzyme induces clotting of plasma (minimum coagulant dose = 4.1 µg) and fibrinogen (minimum coagulant dose = 4.2 µg) in vitro, and promotes defibrin(ogen)ation in vivo (minimum defibrin(ogen)ating dose = 1.0 µg). In addition, when injected intravenously in mice at doses of 5 and 10 µg, it induces a series of behavioral changes, i.e., loss of the righting reflex, opisthotonus, and intermittent rotations over the long axis of the body, which closely resemble the `gyroxin-like' effect induced by other thrombin-like enzymes from snake venoms.

Eficiência da lavagem de carcaças de frango com contaminação fecal aparente, comparada ao corte das áreas afetadas, para redução de contagem bacteriana / Efficiency of washing of carcasses with fecal contamination of chicken apparent, compared to the cutting of the affected areas, to reduce bacterial

A fonte de contaminação com fezes em carcaças de frango ocorre quando o trato intestinal se rompe, é cortado ou quando as fezes são expulsas da carcaça do frango. Amostras de carcaças de frango com contaminação fecal visível foram analisadas, após a retirada com faca da área comprometida. Foram analisadas 1080 carcaças de frango durante 18 dias diferentes de abate, sendo coletadas 10 carcaças por dia em cada ponto de coleta testado, antes e após o chiller. (...) Para as amostras de carcaças de frango coletadas após o chiller, a contagem de Coliformes totais, para o tratamento controle (FDCN), não difere significativamente do tratamento teste FDCL (p>0,05), mas difere do tratamento FDCC (P>0,05) e, o tratamento FDCL, não difere significamente do tratamento FDCC (p>0,05). Para a contagem de Enterobacteriaceae, não há diferença significativa entre o tratamento controle FDCN e FDCL (p>0,05) e ambos, FDCN e FDCL diferem significativamente de FDCC. Para os três tratamentos não houve diferença significativa na pesquisa de E.coli (p=0,30). O procedimento de corte de partes das carcaças contaminadas com fezes, antes do resfriamento, não diminuiu a contaminação microbiológica, comparado com o procedimento de lavagem das carcaças.(AU)

The source of contamination with excrements occurs in chicken carcasses when the digestive tract breaches, it iscut or when the excrements are banished from the chicken carcasses. Carcasses samples with visible fecal contamination were analyzed after removing the affected area with knife and also with water aspersion. 1080 carcasses of chicken were analysed for a period of 18 days collecting 10 carcasses per day in each collecting point.(…) Chicken carcasses after chiller with knife remotion (FDCC) presented significant difference from Total Coliforms (p 0,05); the chicken carcasses with water remotion (FDCL) do not differfrom chicken carcasses after chiller (contra! samples) (FDCN). ForEnterobacteriaceae the FDCN chicken carcasses do not differ from FDCL (p > 0,05) but differ from FDCC (p < 0,05); the FDCL treatment differs significantly from the FDCC treatment (p< 0,05). In the three treatments no difference was presented in theresearch of E.coli. The procedure of knife remotion from carcasses parts contaminated with excrements before the cooling did not reduce significantly the microbiological contamination when compared with the procedure of washing off the carcasses. (AU)

Efeito de diferentes tamanhos de clareiras, sobre o crescimento e a mortalidade de espécies arbóreas, em Moju-PA

Growth and mortality of Sterculia pruriens, Vouacapoua americana, Jacaranda copaia, Protium paraensis, Newtonia suaveolens and Tabebuia serratifolia regarding different gap sizes, had been evaluated in Moju Pará Brazil(2º07'30"S and 2º12'06"S and longitude 48º46'57"W and 48º48'30"W). Nine gaps from forest harvesting had been selected and grouped in small gaps(200m² Área 400m²), medium-sized gaps(400m² Área 600m²) and large gaps(Área>600m²). Around the gaps square plots 5m side had been installed, starting in the gap border to forest inside, according to North, South, East and West directions. In these plots seedlings from natural regeneration of tree species were planted. In the each gap center was installed a square plot of 5m side as testimony, where the plantation was repeated. The total mortality average was 46.9%, not showing significant differences between the small gaps (41,05%) and medium-sized gaps (43,86%) but these had differed from the large gaps (54,96%). The small gaps environment was most favorable for the establishment of most species, except for J.copaia and N. suaveolens whose mortalities had been lesser in the medium-sized gaps, even so has not had significant differences in mortality between the different gap sizes. Mortality varied from 14,5%(S. pruriens) in the small gaps to 70,1%(V. americana) in large gaps, but S. pruriens showed lesser mortality in all sizes of gap. All species had shown greater mortality in large gaps, with remark for V. americana (70,1%), J. copaia (69,1%) and N. suaveolens (58,7%). The mortality are between those finding in literature and for its variability they allow to conclude that if it cannot classify accurately the species in ecological groups only on mortality or survival basis. In terms of growth, however, the results are more conclusive, with evident indication of the medium-sized gaps as the best sites for development of the species, with average height growth of 15cm and base diameter growth 0,16cm, followed for the large gaps and for small ones. In general terms, the height growth average was 11,34cm and the base diameter ones was 0,11cm, with maximum values of 32,5cm (height) and 0,24cm (diameter of base) for J. copaia. P. paraensis and V. americana only had not presented significant differences in height growth regarding to different gap sizes, but the values had been a little bigger in the medium-sized gaps. The species growth and mortality, in two years of monitoring, had presented variations in relation to the different gap sizes. J. copaia and N. suaveolens had presented performance better, as much in terms of mortality as of growth in height and diameter of base in the medium-sized gaps, however this mortality was high in comparison with S. pruriens. It must, therefore, be care when taking silvicultural decisions like as thinning, because it was verified differences even though between species considered of the same ecological group.

Crescimento e mortalidade de Sterculia pruriens, Vouacapoua americana, Jacaranda copaia, Protium paraensis, Newtonia suaveolens e Tabebuia serratifolia, considerando diferentes tamanhos de clareiras, foram avaliados em Moju PA(2º07'30" e 2º12'06" de latitude Sul e 48º46'57" e 48º48'30" de longitude a Oeste de Greenwitch). Selecionou-se nove clareiras da exploração florestal, que foram agrupadas em pequenas (200m² Área 400m2­), médias(400m² Área 600m²) e grandes (>600m²). Em seu torno instalou-se parcelas quadradas de cinco metros de lado, nas direções Norte, Sul, Leste e Oeste, onde foram plantados indivíduos da regeneração natural de espécies arbóreas. No centro de cada clareira foi instalada uma parcela de 5m X 5m como comparador. A média da mortalidade total foi de 46,9%, não havendo diferenças entre as clareiras pequenas(41,05%) e médias(43,86%), mas estas diferiram das grandes(54,96%). As clareiras pequenas são mais propícias para a maioria das espécies, exceto para J. copaia e N. suaveolens, cujas mortalidades foram menores nas clareiras médias. A mortalidade variou de 14,5%(S. pruriens) nas clareiras pequenas a 70,1%(V. americana) em clareiras grandes, sendo que S. pruriens mostrou menor mortalidade em todos os tamanhos de clareiras. As espécies morreram mais em clareiras grandes. A mortalidade está entre os valores encontrados na literatura, permitindo concluir que não se pode classificar com precisão as espécies em grupos ecológicos somente com base na mortalidade ou sobrevivência. Em termos de crescimento, os resultados indicam que os melhores sítios para desenvolvimento das espécies são as clareiras médias, seguidos pelas clareiras grandes e pequenas. Em termos gerais, a média de crescimento em altura foi de 11,34cm e de 0,11cm em diâmetro de base, com valores maiores para J. copaia. Somente V. americana e P. paraenses não apresentaram diferenças significativas no crescimento em altura em relação aos diferentes tamanhos de clareiras. Os valores de crescimento e mortalidade das espécies apresentaram variações em relação aos diferentes tamanhos de clareiras. J. copaia e N. suaveolens apresentaram melhor desempenho, tanto em termos de mortalidade como de crescimento em altura e diâmetro de base nas clareiras médias, todavia essa mortalidade foi elevada em comparação com S. pruriens.

Diagnóstico ultra-sonográfico do momento ovulatório em búfalas (Bubalus bubalis) inseminadas no estro espontâneo e induzido

The aim of this experiment was to determine the interval between the beginning of the spontaneous or induced oestrus and the ovulation in females buffaloes using ultrasonography. This will be useful in the determination of the most proper moment for the pre-fixed artificial insemination. In the reproductive season, autumn in the South of Brazil (march-june), 132 clicling females were divided in 3 groups: Group A: 53 females were treated with auricular subcutaneous implant of norgestomet or intravaginal device of progesterone. In the moment that the devices were removed, 250mg of cloprostenol were applied for intravulvar submucosis (ivsm). Group B: 48 buffaloes females were treated twice with 250mg of cloprostenol for ivsm with interval of 11 days. Group C: 31 buffaloes females remained without any treatment (control group). All of them were inseminated in the moment that was observed the biggest diameter of pre-ovulatory follicle determinated by ultrasonography. In the 3 groups, there was significative difference between pregnant and non-pregnant females in the oestrus-ovulation interval and in the A.I.-ovulation interval. The pregnancy rates were 41.5%, 52.1% and 54.8% in the groups A, B amd C, respectively. The variation in the oestrus-ovulation in buffaloes is the major obstacle to achieve high pregnancy rates using pre-fixed artificial insemination in spontaneous and induced oestrus.

O objetivo deste experimento foi determinar o intervalo entre o início do estro induzido ou espontâneo e a ovulação em fêmeas bubalinas (Bubalus bubalis) com o auxílio da ultra-sonografia, o que permitirá a determinação de um horário mais apropriado para a I.A. pré-fixada. Nos meses de março a junho, outono no sul do Brasil (época reprodutiva dos bubalinos), 132 fêmeas adultas ciclando foram divididas em 3 grupos experimentais: Grupo A - 53 fêmeas foram tratadas com implante subcutâneo de Norgestomet ou espiral intravaginal contendo progesterona. Na retirada dos dispositivos, foi aplicado 250mg de cloprostenol pela via intra-submucosa vulvar (i.s.m.v.), Grupo B - 48 búfalas foram tratadas com dupla aplicação de 250mg de cloprostenol pela via i.s.m.v. com intervalo de 11 dias e Grupo C - 31 búfalas foram consideradas testemunhas, sem tratamento. Todas as búfalas foram inseminadas no momento da observação do maior diâmetro do folículo pré-ovulatório, detectado por ultra-sonografia, durante o estro. Após o diagnóstico de prenhez, constatou-se, nos três tratamentos, que houve diferença significativa entre as búfalas prenhes e vazias no período compreendido entre o início do estro até o momento da ovulação e no período entre a I.A.e a ovulação. Os índices de prenhez foram de 41,5%, 52,1% e 54,8% nos grupos A, B e C, respectivamente. A variação no intervalo estro-ovulação nas búfalas é uma barreira para a obtenção de taxas de prenhez por I.A. pré-fixada comparáveis à monta natural, tanto no estro induzido através de progesterona e prostaglandina F2 alfa como no estro espontâneo.