Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...(AU)

Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...

Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil / Mycoplasma agalactiae em sêmen e leite de caprinos do estado de Pernambuco

In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR) was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39) of the semen samples and 3.7% (3/81) of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil. (AU)

Em caprinos e ovinos as micoplasmoses causam sérias perdas econômicas associadas com poliartrites, mastites, agalaxia, conjuntivite, pneumonias e falhas reprodutivas. Esta última pode envolver repetição de cio, vulvovaginite granular, infertilidade e abortos. O objetivo desse estudo foi verificar a ocorrência de Mycoplasma agalactiae (Ma) em sêmen e leite de caprinos naturalmente infectados procedentes de regiões semiáridas do Estado de Pernambuco, Nordeste do Brasil. Foram usadas 39 amostras de sêmen e 81 de leite, as quais foram submetidas à extração do DNA genômico usando um kit comercial, seguindo as instruções do fabricante. A reação da PCR foi realizada de acordo com protocolo previamente descrito na literatura. Os resultados revelaram a presença de DNA de Ma nas amostras de sêmen com uma frequência de 17,9% (7/39) e no leite a frequência encontrada foi de 3,7% (3/81). Os resultados obtidos no presente estudo confirmam a eliminação de DNA de Ma nas amostras de sêmen e leite analisadas. A presença deste agente nos rebanhos caprinos pode ser considerada um risco para doenças reprodutivas e surtos de agalaxia contagiosa na região Nordeste do Brasil. (AU)

Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil / Mycoplasma agalactiae em sêmen e leite de caprinos do estado de Pernambuco

In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR) was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39) of the semen samples and 3.7% (3/81) of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil.

Em caprinos e ovinos as micoplasmoses causam sérias perdas econômicas associadas com poliartrites, mastites, agalaxia, conjuntivite, pneumonias e falhas reprodutivas. Esta última pode envolver repetição de cio, vulvovaginite granular, infertilidade e abortos. O objetivo desse estudo foi verificar a ocorrência de Mycoplasma agalactiae (Ma) em sêmen e leite de caprinos naturalmente infectados procedentes de regiões semiáridas do Estado de Pernambuco, Nordeste do Brasil. Foram usadas 39 amostras de sêmen e 81 de leite, as quais foram submetidas à extração do DNA genômico usando um kit comercial, seguindo as instruções do fabricante. A reação da PCR foi realizada de acordo com protocolo previamente descrito na literatura. Os resultados revelaram a presença de DNA de Ma nas amostras de sêmen com uma frequência de 17,9% (7/39) e no leite a frequência encontrada foi de 3,7% (3/81). Os resultados obtidos no presente estudo confirmam a eliminação de DNA de Ma nas amostras de sêmen e leite analisadas. A presença deste agente nos rebanhos caprinos pode ser considerada um risco para doenças reprodutivas e surtos de agalaxia contagiosa na região Nordeste do Brasil.