Impact of improper sample handling on Cobas CT/NG testing platform with dual swab sample collection kit for detecting Chlamydia trachomatis and Neisseria gonorrhoeae.

IMPORTANCE: The importance of this observation lies in its potential to directly impact testing outcomes and patient care. By identifying improper sample handling as a contributing factor to a substantial number of invalid results, we emphasize the need for meticulous adherence to recommended protocols during sample collection. Laboratories that overlook or are unaware of such deviations may inadvertently compromise the reliability and efficacy of their diagnostic processes, leading to misdiagnoses, delayed treatment, and patient dissatisfaction.

Experiences of grief and moral sentiments in survivors of the armed conflict in Medellín, Colombia.

The objective of the study is to understand the experience of grief and moral sentiments in survivors of the armed conflict in the city of Medellín, Colombia, through qualitative research. In total, 32 people affected by different victimising acts in the context of the armed conflict participated. There is a direct relationship between moral sentiments and the processes of handling and processing loss. Moral sentiments frame the experience of emotions in the victim, after the events have occurred, which influence the way in which the pain experienced is processed. These sentiments permeate the processes of dealing with the incident in survivors, the ways in which they perceive themselves and the perpetrators, and the social stances they adopt to face life. We conclude that moral factors play a role in the emotional recovery of victims and are indicative the possibility or difficulty of processing the events that occurred.

A 'worthy disciple of Galen', 'ardent sportsman' and 'expert swordsman': Henry Kipping (1726-1785) apothecary and surgeon at Brighton, England.

Henry Kipping (1726-1785) was an apothecary and surgeon in Brighton, England. Here we present a series of contemporary references to Kipping from newspaper, book, archive and web-based resources. Some relate to his medical practice (resuscitating a 'drowned' elderly physician and a fisherman, bleeding a member of parliament who had fallen from his horse and praising a nostrum for the 'gravel and stone'). Social references include a duel with an army officer whose sword Kipping confiscated. Kipping appears to have been popular, connected with members of Brighton's high society and passionate about traditional past times, e.g. swordsmanship, horse riding and hunting on the Sussex downs. Indeed, Kipping's horse ran in the earliest known horse race in Brighton (1770). He was consulted by notable local residents including the Thrale family of Brighton and Lady Wilhelmina Shelley (the latter evidenced by Kipping partaking in her funeral procession in 1772). Kipping lived and practised at 28 West street, a road most famous for its (now lost) George Inn where King Charles II stayed just prior to his escape to Normandy. Kipping comes across as a colourful and eccentric clinician.

Higher thyroid hormone has a negative association with lower limb lean body mass in euthyroid older adults: Analysis from the Baltimore Longitudinal study of aging.

Background: Hyperthyroidism is associated with lower lean body mass, as a result of catabolic actions of thyroid hormone. Therefore, higher thyroid hormone levels could be a factor in the development of sarcopenia and age associated functional decline. The relationship between thyroid hormone and muscle mass in ambulatory, euthyroid older adults is not known. Method: We used mixed-effects models to estimate the cross-sectional relationships (accounting for inter-person variability) between thyroid axis hormone measures and lower limb composition or sarcopenia at visits in the Baltimore Longitudinal Study of Aging (BLSA) at which DEXA scans were available and both thyrotropin (TSH) and free thyroxine (FT4) were in the reference range. Analyses were adjusted for levothyroxine use, age, race, sex, BMI, smoking, alcohol intake, cholesterol, and systolic blood pressure. Results: 1442 euthyroid participants (median age 68, 50% female, and 69% white) contributed to 5306 visits from 2003 to 2019. FT4 was negatively associated with lower limb lean mass (beta: 88.49; 95% Confidence Interval (CI): 122.78, -54.20; p < 0.001) and positively associated with sarcopenia (OR: 1.11%, 95% CI: 1.01, 1.22) in the whole cohort. Additionally, higher FT4 was associated with lower leg lean mass (beta: 66.79; 95% CI: 102.24, -31.33; p < 0.001) and sarcopenia (OR:1.09%, 95% CI:1.01, 1.18) in older adults, but not in younger adults alone. Conclusion: In euthyroid older adults, higher FT4 is associated with lower leg lean mass and higher odds of sarcopenia. Understanding the relationship between thyroid hormone and sarcopenia is needed to improve clinical decision-making and avoid functional decline from excess thyroid hormone use in older adults.

Hipólito y la belleza de la muerte (Boceto de relato corto inspirado en hechos reales) / Hipólito e a Beleza da Morte (Esboço de uma história inspirada em acontecimentos reais) / Hippolytus and the Beauty of Death (Sketch of a story inspired by real events)

The aim of this story is to reflect on the death of a pet, the pain it generates and the feelingof emptiness after an absence that comes after a time of coexistence and reciprocal contemplation.At the same time, the narrative is positioned as an instrument to become aware of the feelings between people and their pets, contemplating its use for the progressive approach of children and young people to the phenomenon of death and the feelings it generates. (AU)

El objetivo de este relato consiste en reflexionar sobre la muerte de una mascota, el dolorque genera y la sensación de vacío tras una ausencia sobrevenida tras un tiempo de convivencia ycontemplación recíproca. Paralelamente, se posiciona la narrativa como un instrumento para tomarconciencia de los sentimientos entre las personas y sus mascotas contemplando su empleo para elacercamiento progresivo de niños y jóvenes al fenómeno de la muerte y los sentimientos que genera. (AU)

O objectivo desta história é reflectir sobre a morte de um animal de estimação, a dor quegera e o sentimento de vazio após uma ausência que surge após um tempo de coexistência e contemplação recíproca. Ao mesmo tempo, a narrativa é posicionada como um instrumento para tomarconsciência dos sentimentos entre as pessoas e os seus animais de estimação, contemplando a suautilização para a abordagem progressiva de crianças e jovens ao fenómeno da morte e aos sentimentos que este gera. (AU)

Game Theory-Based Authentication Framework to Secure Internet of Vehicles with Blockchain.

The Internet of Vehicles (IoV) is a new paradigm for vehicular networks. Using diverse access methods, IoV enables vehicles to connect with their surroundings. However, without data security, IoV settings might be hazardous. Because of the IoV's openness and self-organization, they are prone to malevolent attack. To overcome this problem, this paper proposes a revolutionary blockchain-enabled game theory-based authentication mechanism for securing IoVs. Here, a three layer multi-trusted authorization solution is provided in which authentication of vehicles can be performed from initial entry to movement into different trusted authorities' areas without any delay by the use of Physical Unclonable Functions (PUFs) in the beginning and later through duel gaming, and a dynamic Proof-of-Work (dPoW) consensus mechanism. Formal and informal security analyses justify the framework's credibility in more depth with mathematical proofs. A rigorous comparative study demonstrates that the suggested framework achieves greater security and functionality characteristics and provides lower transaction and computation overhead than many of the available solutions so far. However, these solutions never considered the prime concerns of physical cloning and side-channel attacks. However, the framework in this paper is capable of handling them along with all the other security attacks the previous work can handle. Finally, the suggested framework has been subjected to a blockchain implementation to demonstrate its efficacy with duel gaming to achieve authentication in addition to its capability of using lower burdened blockchain at the physical layer, which current blockchain-based authentication models for IoVs do not support.

Political Rivalry During America's Founding Fathers Era and the Bullet that Ended Up in Alexander Hamilton's Upper Lumbar Spine.

On the morning of July 11, 1804, Major General Alexander Hamilton, an American statesman and one of the Founding Fathers of the United States, boarded a barge from Manhattan to the New Jersey shore. There, he met his personal and political opponent, Aaron Burr, for a duel during which Burr shot a bullet that entered Hamilton's torso, fractured a false rib, passed through internal organs, and lodged itself in one of his upper lumbar vertebrae. In this unique historical analysis of a pivotal event in American history, we provide a comprehensive discussion of the spinal injuries sustained by Alexander Hamilton, with special consideration of anatomic details and the ballistics of the time. To the authors' knowledge, this is the first thorough examination of the spinal injuries sustained by the prolific Founding Father and great mind behind the American financial system, Alexander Hamilton.

n-Player Stochastic Duel Game Model with Applied Deep Learning and Its Modern Implications.

This paper provides a conceptual foundation for stochastic duels and contains a further study of the game models based on the theory of stochastic duels. Some other combat assessment techniques are looked upon briefly; a modern outlook on the applications of the theory through video games is provided; and the possibility of usage of data generated by popular shooter-type video games is discussed. Impactful works to date are carefully chosen; a timeline of the developments in the theory of stochastic duels is provided; and a brief literature review for the same is conducted, enabling readers to have a broad outlook at the theory of stochastic duels. A new evaluation model is introduced in order to match realistic scenarios. Improvements are suggested and, additionally, a trust mechanism is introduced to identify the intent of a player in order to make the model a better fit for realistic modern problems. The concept of teaming of players is also considered in the proposed mode. A deep-learning model is developed and trained on data generated by video games to support the results of the proposed model. The proposed model is compared to previously published models in a brief comparison study. Contrary to the conventional stochastic duel game combat model, this new proposed model deals with pair-wise duels throughout the game duration. This model is explained in detail, and practical applications of it in the context of the real world are also discussed. The approach toward solving modern-day problems through the use of game theory is presented in this paper, and hence, this paper acts as a foundation for researchers looking forward to an innovation with game theory.

Experiences of Grief and Moral Sentiments in Survivors of the Armed Conflict in Medellín, Colombia. / Experiencias de duelo y sentimientos morales en sobrevivientes del conflicto armado en Medellín, Colombia.

The objective of the study is to understand the experience of grief and moral sentiments in survivors of the armed conflict in the city of Medellín, Colombia, through qualitative research. In total, 32 people affected by different victimising acts in the context of the armed conflict participated. There is a direct relationship between moral sentiments and the processes of handling and processing loss. Moral sentiments frame the experience of emotions in the victim, after the events have occurred, which influence the way in which the pain experienced is processed. These sentiments permeate the processes of dealing with the incident in survivors, the ways in which they perceive themselves and the perpetrators, and the social stances they adopt to face life. We conclude that moral factors play a role in the emotional recovery of victims and are indicative the possibility or difficulty of processing the events that occurred.

Low skeletal muscle mass is associated with liver fibrosis in individuals with type 2 diabetes and nonalcoholic fatty liver disease.

BACKGROUND AND AIM: Type 2 diabetes (T2D) and low skeletal muscle mass (SMM) are associated with increased risk of nonalcoholic fatty liver disease (NAFLD). However, data regarding the association between low SMM and NAFLD-related liver fibrosis in individuals with T2D are scarce. Therefore, we aimed to investigate the association between low SMM and liver fibrosis in individuals with T2D and NAFLD. METHODS: Controlled attenuation parameter (CAP) of ≥ 248 dB/m was taken as cutoff suggesting NAFLD. Clinically relevant liver fibrosis and advanced liver fibrosis were defined as liver stiffness measurement (LSM) by transient elastography (TE) of ≥ 8.0 and ≥ 9.6 kPa, respectively. SMM was measured using dual energy X-ray absorptiometry (DEXA). Low SMM was defined as appendicular SMM index of < 7.0 kg/m2 for men and < 5.4 kg/m2 for women. RESULTS: Of the 487 consecutive patients with T2D, 366 (75.1%) had NAFLD. Among individuals with NAFLD, 118 (32.2%) and 64 (17.5%) had clinically relevant liver fibrosis and advanced liver fibrosis, respectively. Low SMM was diagnosed in 78 (21.3%) individuals with NAFLD. Patients with low SMM were older (56.1 vs 52.8 years) and had longer duration of diabetes (10.3 vs 8.1 years). Low SMM was an independent risk factor associated with clinically relevant liver fibrosis (P = 0.002) and advanced liver fibrosis (P ≤ 0.0001). Associations between low SMM and clinically relevant- and advanced liver fibrosis were maintained even after sequential adjustment for confounding variables through the multivariate regression analysis. CONCLUSIONS: Low SMM is independently associated with liver fibrosis in individuals with T2D and NAFLD.

Clínica Psicoanalítica en la Infertilidad: Epigenética y Duelo / Psychoanalytic Clinic in Infertility: Epigenetics and Duel

En el presente texto nos proponemos analizar de qué manera se juega en las Técnicas de Reproducción humana asistida, los descubrimientos más recientes en relación a la epigenética y su impacto en el atravesamiento del duelo genético. Además, se establecerá, a partir de un caso clínico, qué ofrece el psicoanálisis para propiciar el trabajo del duelo en la clínica de la infertilidad a diferencia de otros tipos de tratamiento psicoterapéutico

In the present text we propose to analyze how, in the assisted human reproduction technology, the most recent discoveries in epigenetics have an impact on the path of genetic dueling. On the other hand, we define from a clinical case, what psychoanalysis offers to favor mourning in the clinic of infertility compared to other types of psychotherapeutic treatments

El duelo en tiempos de pandemia: la muerte escrita / Grief in times of pandemic: written death

En tiempos de pandemia los rituales funerarios quedan alterados y en ciertos sitios, suprimidos. los alcances subjetivos de este impedimento no son todavía visibles. No obstante, la formas de despedida y acompañamiento no quedan canceladas. La palabra encuentra los modos de alojar al dolor mostrado su eficacia en el campo de lo humano. Es necesario acentuar estos modos vicarios de la despedida porque de ellos depende el destino del duelo mismo

In times of pandemic, funeral rituals are altered and, in certain places, suppressed. the subjective implications of this impediment are not yet visible. However, the forms of farewell and accompaniment are not canceled. The word finds ways to accommodate pain by showing its effectiveness in the field of the human. It is necessary to accentuate these vicarious ways of parting because the fate of the duel itself depends on them

Duelo perinatal: perspectivas de los profesionales de la salud / Perinatal grief: perspectives of health professionals

INTRODUCCIÓN: Los profesionales tienen un rol significativo en el proceso del duelo perinatal, y la relación que construyan con los padres será decisiva para facilitar u obstaculizar su cuidado. OBJETIVO: Comprender la perspectiva de los profesionales de la salud con relación al duelo perinatal. MÉTODO: Se realizó una búsqueda bibliográfica en las bases de datos CINAHL, CUIDEN, PsycINFO, PubMed y Google Académico. Se realizó un análisis temático de estos, permitiendo delimitar categorías temáticas comunes. RESULTADOS: Se obtuvieron 46 artículos cientificos con menos de 10 años de antiguedad. Se identificaron tres categorias para abordar la experiencia vivida: 1) Sentimientos experimentados por los profesionales, como ansiedad, culpa, resignación, síntomas físicos, frustración y evitación; 2) Necesidades sentidas de apoyo, entre estas, soporte emocional, capacitación formal y experiencia laboral que permitan afrontar de mejor forma el duelo con las familias y el equipo; 3) Facilitadores del proceso, donde se destacan la capacitación, años de trayectoria profesional, confianza en si mismo, apoyo de los pares, equipo multidisciplinario, creencia religiososa y apoyo institucional. CONCLUSIÓN: El duelo perinatal es una situación dramática para los padres, los cuales demandan un cuidado sensible. Y los profesionales desempeñan un rol clave en el apoyo integral a los padres, siendo un proceso exigente tanto en lo personal como en lo profesional. Se sugiere formación de pregrado y postgrado, fomentando el diálogo y apoyo entre los equipos. De esta forma se beneficiarán los padres, su familia y los profesionales, los cuales podrán otorgar un cuidado efectivo y de calidad en situación de duelo perinatal.

INTRODUCCIÓN: Professionals play a significant role in the perinatal mourning. The relationship they established with parent in this process is critical in facilitating or hindering the quality of health care in this context. OBJETIVO: to understand the perspective of health care professionals in relation to perinatal grief. METHOD: A literature review was carried out in CINAHL, CUIDEN, PsycINFO, PubMed and the Google Scholar databases. A thematic analysis of the selected articles was carried out, allowing to define common thematic categories in these articles. RESULTS: 46 scientific articles, published in the last ten years, were obtained. Three topics were identified: 1) feelings experienced by professionals, including anxiety, guilt, anger, resignation, physical symptoms, frustration, avoidance and emotional pain; 2) needs related to support, such as emotional support, formal training and work experience that allows for a better approach with families and parents; and 3) facilitators of the process, where training, years of professional experience, self-confidence, peer support, multidisciplinary team, religious beliefs and institutional support stand out. CONCLUSION: Perinatal grief is a complex situation for parents who demand sensitive care. Professionals play a key role in comprehensive support for them, being a demanding process both personally and professionally. Undergraduate and postgraduate training is suggested, encouraging dialogue and support among teams. This would benefit family and professionals, providing effective and quality care in a situation of perinatal grief.

The Interaction between Phagocytes and Streptococcus agalactiae (GBS) Mediated by the Activated Complement System is the Key to GBS Inducing Acute Bacterial Meningitis of Tilapia.

Streptococcus agalactiae is an important pathogen for tilapia meningitis. Most of the infected tilapia die rapidly in production, when the way to study the pathogenic mechanism of bacteria on host through chronic infection in laboratory is not comprehensive and accurate enough to elucidate the real pathogenic mechanism. The objective of this study was to investigate the mechanism of acute bacterial meningitis of tilapia caused by Streptococcus agalactiae (GBS), and provide a theoretical basis for its prevention and treatment. Duel RNA-seq, proteome analysis, histopathological analysis, plasma biochemical indexes, and blood routine examination were performed on tilapias infected with fish-derived GBS attenuated strain YM001 and its parental virulent strain HN016. The results showed that the contents of white blood cell (WBC), monocytes (MON), and neutrophil (NEU) were significantly lower in the HN016 group compared to that in the YM001 group (p < 0.05). Histopathological examination showed that there were partially lesions in the examined tissues of tilapia infected by HN016, while no obvious histopathological changes occurred in the YM001 group. The differential expressed genes (DEGs) and differential expressed proteins (DEPs) between YM001 and HN016 were mainly enriched in the beta-lactam resistance pathway (oppA1, oppA2, oppB, oppC, oppD, oppF, and mrcA). The DEGs DEPs between YM001-brain and HN016-brain were mainly enriched in the complement and coagulation cascades signaling pathway (C2a, c4b, c3b, c7, CD59, ITGB2, and ITGAX). The present study indicates that the interaction between phagocytes and GBS mediated by the activated complement system is the key to GBS inducing tilapia acute bacterial meningitis. The low survival ability caused by reduced ß-lactam antibiotics resistance is one of the important reasons for why YM001 lost its pathogenicity to tilapia.

Poesía diaria : veinte años de los recordatorios de detenidos-desaparecidos en Página-12 / Twenty years of reminders of missing relatives in Page / 12

Desde 1988 el diario Página/12 ofrece a familiares y amigos de personas secuestradas y asesinadas durante la última dictadura cívico-militar un espacio para recordar y homenajear a sus seres queridos que ya no están. Se trata de una iniciativa que, por sus características y permanencia en el tiempo, resulta absolutamente conmovedora y original. Este artículo presenta el valor de este gesto a través de su relación con la escritura como vía para la simbolización de la muerte ante la ausencia del cuerpo.

Since 1988, the Argentine newspaper Página / 12 offers relatives and friends of people kidnapped and murdered during the last civil-military dictatorship a space to remember and honor their loved ones who are no longer there. It is an initiative that, due to its characteristics and permanence over time, is absolutely moving and original. This article presents the value of this gesture through its relationship with writing as a way to symbolize death in the absence of the body.

El derecho a la muerte / The right to written death

Este artículo fue publicado originalmente en el número 42 de la revista Conjetural, e incluido luego, con modificaciones, como Postfacio del libro Epitafios : el derecho a la muerte escrita (Grupo Edit. Norma, Buenos Aires, 2005). Se lo reproduce aquí en una versión completa cedida por el autor para este número de la revista de la UBA. Se consignan a continuación sus apartados : Duelo y transmisión / Lo siniestro perdura : un funeral sin resolver / La política de los huesos : No entregar los cadáveres / La estetización de la muerte / El espejismo de restos.

This article was originally published in issue 42 of Conjetural magazine, and then included, with modifications, such as Postface of the book Epitafios : the right to written death (Grupo Edit, Norma, Buenos Aires, 2005). It is reproduced here in a complete version provided by the author for this issue of the UBA magazine. Its sections are consigned below : Grief and transmission / The sinister endures : an unresolved funeral / The policy of the bones : Do not deliver the corpses / The aestheticization of death / The mirage of remains.

D3 -Creatine dilution and the importance of accuracy in the assessment of skeletal muscle mass.

Sarcopenia has been described as the age-associated decrease in skeletal muscle mass. However, virtually every study of sarcopenia has measured lean body mass (LBM) or fat free mass (FFM) rather than muscle mass, specifically. In a number of published sarcopenia studies, LBM or FFM is referred to as muscle mass, leading to an incorrect assumption that measuring LBM or FFM is an accurate measure of muscle mass. As a result, the data on the effects of changes in LBM or FFM in older populations on outcomes such as functional capacity, disability, and risk of injurious falls have been inconsistent resulting in the conclusion that muscle mass is only weakly related to these outcomes. We review and describe the assumptions for the most commonly used measurements of body composition. Dual-energy X-ray absorptiometry (DXA) has become an increasingly common tool for the assessment of LBM or FFM and appendicular lean mass as a surrogate, but inaccurate, measurement of muscle mass. Other previously used methods (total body water, bioelectric impedance, and imaging) also have significant limitations. D3 -Creatine (D3 -Cr) dilution provides a direct and accurate measurement of creatine pool size and skeletal muscle mass. In a recent study in older men (MrOS cohort), D3 -Cr muscle mass was associated with functional capacity and risk of injurious falls and disability, while assessments of LBM or appendicular lean mass by DXA were only weakly or not associated with these outcomes. Inaccurate measurements of muscle mass by DXA and other methods have led to inconsistent results and potentially erroneous conclusions about the importance of skeletal muscle mass in health and disease. The assessment of skeletal muscle mass using the D3 -Cr dilution method in prospective cohort studies may reveal sarcopenia as a powerful risk factor for late life disability and chronic disease.

Be right back | Vuelvo enseguida: ¿Qué es lo que no vuelve? / Be right back What does not come back?

En este trabajo se utilizará como recorte situacional el capítulo "Be right back" de la serie "Black Mirror", para repreguntarnos bajo qué condiciones la utilización de una biotecnología promueve el desarrollo de lo simbólico o por el contrario, favorece su aplastamiento. Es en este sentido donde aparecerán presentados los avatares de la intersección entre el saber científico y el campo subjetivo, en relación al orden singular de un duelo

This paper will be used as situational cut the chapter "Be right back" from Black Mirror´s series, to cross-examine us under what conditions the use of a biotechnology promotes the development of the symbolic or conversely, promotes its crushing. It is in this sense where appear presented the vicissitudes of the intersection between the scientific knowledge and subjective field, in relation to duel´s unique order

Diseño y análisis psicométrico del inventario IPDP para evaluar el proceso de duelo patológico en adultos / Design and analysis of the IPDP psychometric inventory to evaluate pathological grief in adults

Resumen Se diseñó y realizó el análisis psicométrico de un instrumento para evaluar duelo patológico en adultos. El estudio es de tipo instrumental, los participantes fueron 235 hombres y mujeres mayores de 18 años. La validación de contenido se realizó con 8 jueces expertos en el tema y en psicometría, para la validez de constructo se realizó un análisis factorial exploratorio. Los ítems se agruparon en cuatro (4) factores que explican el 64.64% de la varianza. En el análisis bajo la teoría de respuesta al ítem (TRI), se encontró que el INFIT de los ítems o estadígrafo de ajuste que captura respuestas no esperadas, cerca del nivel de habilidad del evaluado osciló entre 0.6 y 1.19 y el OUTFIT estadígrafo que captura anomalías lejos de las habilidades calibradas para los evaluados, osciló entre .34 y 1.20, resultados que indican que la prueba cumple con los requerimientos psicométricos exigidos para este tipo de instrumentos. El instrumento aporta a la psicología, puesto que puede ser usado como una medida válida, fiable y útil para el objetivo y contexto para el cual fue elaborado.

Abstract We designed and analyzed an instrument to measure pathological grief in adults with 235 participants. Eight experts in grief and psychometry participated in the content validity phase, and an exploratory factor analysis was performed to assess construct validity. Items were grouped in four factors explaining 64.64% of the variance. The IRT analysis found an infit between 0.6 and 1.19 and the outfit between 0.34 and 1.20. The test fulfills the required psychometric indicators.