ABSTRACT
CONTEXT: Danshen, the dried root and rhizome of Salvia miltiorrhiza Bunge (Labiatae) and honghua, the dried flower of Carthamus tinctorius L. (Compositae) as the herb pair was used to treat cardiovascular diseases (CVD). OBJECTIVE: To study the effects of DHHP on MIRI and mechanisms based on apoptosis and mitochondria. MATERIALS AND METHODS: 36 SD rats (n = 6) were randomly divided into control group (Con), the ischaemia-reperfusion group (IR), positive control (Xinning tablets, XNT, 1 g/kg/d) and DHHP (1.2, 2.4, and 4.8 g/kg/d). Except for Con, the other groups were intragastrically administrated for 5 d, the rat hearts were isolated to establish the MIRI model in vitro for evaluating the effects of DHHP on MIRI. 24 SD rats (n = 6) were randomly divided into Con, IR, DPPH2.4 (2.4 g/kg/d) and DPPH 2.4 + Atractyloside (ATR) (2.4 + 5 mg/kg/d), administered intragastrically for 5 d, then treated with ATR (5 mg/kg/d) by intraperitoneal injection in DPPH2.4 + ATR group, took rat hearts to establish MIRI model in vitro for revealing mechanism. RESULTS: Myocardial infarct sizes were, respectively, 0.35%, 40.09%, 15.84%, 30.13%, concentrations of NAD+ (nmol/gw/w) were 144, 83, 119, and 88, respectively, in Con, IR, DHHP2.4, DHHP2.4 + ATR group. Cleaved caspase-3 were 0.3, 1.6, 0.5 and 1.3% and cleaved caspase-9 were 0.2, 1.1, 0.4 and 0.8%, respectively, in Con, IR, DHHP2.4 and DHHP2.4 + ATR group. The beneficial effects of DHHP on MIRI were reversed by ATR. CONCLUSIONS: The improvement of MIRI by DHHP may be involved in inhibiting MPTP opening, decreasing oxidative damage, alleviating ischaemic injury and inhibiting cardiomyocyte apoptosis.
Subject(s)
Drugs, Chinese Herbal/pharmacology , Myocardial Infarction/prevention & control , Myocardial Reperfusion Injury/drug therapy , Animals , Apoptosis/drug effects , Carthamus tinctorius , Cell Line , Dose-Response Relationship, Drug , Drugs, Chinese Herbal/administration & dosage , Male , Mitochondria/drug effects , Mitochondrial Permeability Transition Pore/metabolism , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/pathology , Random Allocation , Rats , Rats, Sprague-Dawley , Salvia miltiorrhizaABSTRACT
Regulations for the Implementation of the Drug A dministration Law of the People's Republic of China made status quo administration centralized large-scale cultivation farming, quality controls and complied with the provisions of herbal variety conditions of departments of the State Drug Administration. This article summarized the current implementation of approval number on decoction pieces and extracts of Chinese medicine. It also analyzed the faint classification between extracts of Chinese medicine of implementing approval document with those of imple-menting approval document for chemical drugs. Further details of implementing and improving approval document for decoction pieces and extracts of Chinese medicine were also discussed.
