Expression of DDSR1 Long Non-Coding RNA and Genes Involved in the DNA Damage Response in Sperm with DNA Fragmentation.

The molecular mechanism responsible for sperm DNA fragmentation is not fully understood. Therefore, identifying genes related to the response to DNA damage is an important area of research. Recently, the role of long non-coding RNAs (LncRNAs), especially DNA damage-sensitive RNA1 (DDSR1) in male infertility has been highlighted. In this research, a protein-protein interaction network (PPIN) was constructed using the STRING database, and functional classification was conducted using webgestalt servers. Subsequently, a group of 40 males with a high degree of sperm DNA fragmentation (DFI ≥ 25%) was compared to a control group of 20 healthy males with a normal sperm DNA fragmentation rate (DFI < 25%). To assess gene expression, real-time polymerase chain reaction (PCR) analysis was performed on DNA samples obtained from both healthy and infertile males. Our findings revealed that infertile men with an abnormal DFI index showed significantly lower expression levels of the long noncoding RNA DDSR1, as well as the genes BRCA1, MRE11A, RAD51, and NBN, compared to the control group. Pathway analysis of the network proteins using Reactome indicated involvement in crucial cellular processes such as the cell cycle, DNA repair, meiosis, reproduction, and extension of telomeres. In conclusion, the downregulation of LncRNA and genes associated with the DNA damage response in males with an abnormal DFI suggests that these factors may contribute to the development of sperm DNA fragmentation and could potentially serve as diagnostic markers for further investigation in therapeutic interventions in the future.

Ethics of Residency Programs and Fellowships that Electively Withdraw from the NRMP Match.

The National Resident Matching Program (NRMP), known as "The Match (TM)," facilitates the placement of medical graduates into residency and fellowship programs in the United States. Programs may opt out of TM for various reasons. The selective and intermittent withdrawal of programs from TM raises ethical concerns surrounding fairness, transparency, beneficence, autonomy, and justice for applicants. We discuss these issues, present the history behind TM, and offer suggestions to promote fairness.

Edwards curve digital signature algorithm for video integrity verification on blockchain framework.

Within the field of forensic science, video integrity is an essential component that ensures the validity and dependability of visual evidence that is essential for court cases. Maintaining the integrity of justice in a time when digital modification tools are easily accessible requires making sure that video recordings are unaltered. Video footage is carefully inspected by forensic analysts who use advanced tools to look for indications of manipulation or change. This paper presents a novel, lightweight approach for verifying the integrity of video data. The proposed method utilizes the blockchain and the Edwards Curve Digital Signature Algorithm, coupled with the BLAKE2b hash function. Signatures are generated for video clips with a predetermined size called video segments and stored in blocks chronologically. The signature of the previous block is stored in the current block to add another layer of security. At the time of validation, these signatures are verified. Experimental results show that the proposed method outperforms state-of-the-art methods in both speed and security. Our method is capable of identifying any type of forgery on any video file, by anyone, at any given moment, with insignificant additional storage requirements. Furthermore, our security analysis shows that our method is resilient against various types of attacks, including collision attacks, key substitution attacks, side-channel attacks, and chosen message attacks. The proposed lightweight video integrity verification method is better suited for use in resource constrained devices.

How to fight fake papers: a review on important information sources and steps towards solution of the problem.

Scientific fake papers, containing manipulated or completely fabricated data, are a problem that has reached dramatic dimensions. Companies known as paper mills (or more bluntly as "criminal science publishing gangs") produce and sell such fake papers on a large scale. The main drivers of the fake paper flood are the pressure in academic systems and (monetary) incentives to publish in respected scientific journals and sometimes the personal desire for increased "prestige." Published fake papers cause substantial scientific, economic, and social damage. There are numerous information sources that deal with this topic from different points of view. This review aims to provide an overview of these information sources until June 2024. Much more original research with larger datasets is needed, for example on the extent and impact of the fake paper problem and especially on how to detect them, as many findings are based more on small datasets, anecdotal evidence, and assumptions. A long-term solution would be to overcome the mantra of publication metrics for evaluating scientists in academia.

Direct and deferred sediment-transport events and seafloor disturbance induced by trawling in submarine canyons.

Bottom trawling on marine environments can drastically modify seafloor geomorphology and sedimentary dynamics not only on the fishing grounds but also in adjacent downslope regions, particularly in submarine canyons environments, which are hotspots of benthic biomass and productivity in the deep sea. When this type of fishery occurs along submarine canyon flanks, it can induce sediment gravity flows that descend along tributary gullies towards the main canyon axis. However, these flows had only been clearly identified in the Palamós Canyon, where they could be recorded synchronously with the passage of the trawling fleet. In this study we also recorded trawl-induced sediment gravity flows in the Blanes Canyon, both synchronously and asynchronously with the passage of trawlers. Increases in particulate matter fluxes in other trawled submarine canyons occurring in absence of natural triggering mechanisms, were not directly associated with bottom trawling because of the lack of direct synchronicity of these events with this human activity. Here we show, however, that the practice of bottom trawling along canyon flanks can not only resuspend and directly trigger sediment gravity flows, but they can also pile up disturbed sediment on steep areas, which can become unstable and collapse afterwards, asynchronically with the passage of trawlers. Our study provides evidence that sediment gravity flows in submarine canyons affected by bottom trawling, where the causal mechanisms are presently unidentified, may potentially be linked to instabilities in sediment originating from recurrent bottom trawling, which can precondition these events.

An integrated network pharmacology approach reveals that Ampelopsis grossedentata improves alcoholic liver disease via TLR4/NF-κB/MLKL pathway.

BACKGROUND: Alcohol-related liver damage is the most prevalent chronic liver disease, which creates a heavy public health burden worldwide. The leaves of Ampelopsis grossedentata have been considered a popular tea and traditional herbal medicine in China for more than one thousand years, and possess anti-inflammatory, antioxidative, hepatoprotective, and antiviral activities. PURPOSE: We explored the protective effects of Ampelopsis grossedentata extract (AGE) against chronic alcohol-induced hepatic injury (alcoholic liver disease, ALD), aiming to elucidate its underlying mechanisms. METHODS: Firstly, UPLC-Q/TOF-MS analysis and network pharmacology were used to identify the constituents and elucidate the potential mechanisms of AGE against ALD. Secondly, C57BL/6 mice were pair-fed the Lieber-DeCarli diet containing either isocaloric maltodextrin or ethanol, AGE (150 and 300 mg/kg/d) and silymarin (200 mg/kg) were administered to chronic ethanol-fed mice for 7 weeks to evaluate the hepatoprotective effects. Serum biochemical parameters were determined, hepatic and ileum sections were used for histologic examination, and levels of inflammatory cytokines and oxidative stress in the liver were examined. The potential molecular mechanisms of AGE in improving ALD were demonstrated by RNA-seq, Western blotting analysis, and immunofluorescence staining. RESULTS: Ten main constituents of AGE were identified using UPLC-Q/TOF-MS and 274 potential ALD-related targets were identified. The enriched KEGG pathways included Toll-like receptor signaling pathway, NF-κB signaling pathway, and necroptosis. Moreover, in vivo experimental studies demonstrated that AGE significantly reduced serum aminotransferase levels and improved pathological abnormalities after chronic ethanol intake. Meanwhile, AGE improved ALD in mice by down-regulating oxidative stress and inflammatory cytokines. Furthermore, AGE notably repaired damaged intestinal epithelial barrier and suppressed the production of gut-derived lipopolysaccharide by elevating intestinal tight junction protein expression. Subsequent RNA-seq and experimental validation indicated that AGE inhibited NF-κB nuclear translocation, suppressed IκB-α, RIPK3 and MLKL phosphorylation and alleviated hepatic necroptosis in mice. CONCLUSION: In this study, we have demonstrated for the first time that AGE protects against alcoholic liver disease by regulating the gut-liver axis and inhibiting the TLR4/NF-κB/MLKL-mediated necroptosis pathway. Therefore, our present work provides important experimental evidence for AGE as a promising candidate for protection against ALD.

Itga5-PTEN signaling regulates striatal synaptic strength and motor coordination in Parkinson's disease.

Background: Parkinson's disease (PD) is marked by the loss of dopaminergic neurons in the substantia nigra pars compacta, leading to motor and cognitive dysfunctions. The molecular mechanisms underlying synaptic alterations in PD remain elusive, with a focus on the role of Itga5 in synaptic integrity and motor coordination and TAT-Itga5 was designed to suppress PTEN activity in this investigation. Methods: This study utilized MPTP-induced PD animal models to investigate the expression and role of Itga5 in the striatum. Techniques included quantitative PCR, Western blotting, immunostaining, CRISPR-CasRx-mediated knockdown, electrophysiological assays, behavioral tests, and mass spectrometry. Results: Itga5 expression was significantly reduced in MPTP-induced PD models. In these models, a marked decrease in dendritic spine density and a shift towards thinner spines in striatal GABA neurons were observed, suggesting impaired synaptic integration. Knockdown of Itga5 resulted in reduced dendritic branching, decreased mushroom spines, and increased thin spines, altering synaptic architecture. Electrophysiological analyses revealed changes in action potential and spontaneous excitatory postsynaptic currents, indicating altered synaptic transmission. Motor behavior assessments showed that Itga5 deficiency led to impairments in fine motor control and coordination. Furthermore, Itga5 was found to interact with PTEN, affecting AKT signaling crucial for synaptic development and motor coordination. Conclusion: The study demonstrates that Itga5 plays a critical role in maintaining synaptic integrity and motor coordination in PD. The Itga5-PTEN-AKT pathway represents a potential therapeutic target for addressing synaptic and motor dysfunctions in PD.

Addressing researcher fraud: retrospective, real-time, and preventive strategies-including legal points and data management that prevents fraud.

Researcher fraud is often easy and enticing in academic research, with little risk of detection. Cases of extensive fraud continue to occur. The amount of fraud that goes undetected is unknown and may be substantial. Three strategies for addressing researcher fraud are (a) retrospective investigations after allegations of fraud have been made, (b) sting operations that provide conclusive evidence of fraud as it occurs, and (c) data management practices that prevent the occurrence of fraud. Institutional and regulatory efforts to address researcher fraud have focused almost exclusively on the retrospective strategy. The retrospective approach is subject to controversy due to the limitations of post-hoc evidence in science, the difficulty in establishing who actually committed the fraud in some cases, the application of a legal standard of evidence that is much lower than the usual standards of evidence in science, and the lack of legal expertise by scientists investigating fraud. The retrospective strategy may be reliably effective primarily in cases of extensive, careless fraud. Sting operations can overcome these limitations and controversies, but are not feasible in many situations. Data management practices that are effective at preventing researcher fraud and unintentional errors are well-established in clinical trials regulated by government agencies, but appear to be largely unknown or unimplemented in most academic research. Established data management practices include: archiving secure copies of the raw data, audit trails, restricted access to the data and data collection processes, software validation, quality control checks, blinding, preregistration of data processing and analysis programs, and research audits that directly address fraud. Current discussions about data management in academic research focus on sharing data with little attention to practices that prevent intentional and unintentional errors. A designation or badge such as error-controlled data management could be established to indicate research that was conducted with data management practices that effectively address intentional and unintentional errors.

Regulation of Precise DNA Repair by Nuclear Actin Polymerization: A Chance for Improving Gene Therapy?

Although more difficult to detect than in the cytoplasm, it is now clear that actin polymerization occurs in the nucleus and that it plays a role in the specific processes of the nucleus such as transcription, replication, and DNA repair. A number of studies suggest that nuclear actin polymerization is promoting precise DNA repair by homologous recombination, which could potentially be of help for precise genome editing and gene therapy. This review summarizes the findings and describes the challenges and chances in the field.

Modulation of Bronchial Epithelial Barrier Integrity by Low Molecular Weight Components from Birch Pollen.

Pollen, in addition to allergens, comprise low molecular weight components (LMC) smaller than 3 kDa. Emerging evidence indicates the relevance of LMC in allergic immune responses. However, the interaction of birch pollen (BP)-derived LMC and epithelial cells has not been extensively studied. We investigated epithelial barrier modifications induced by exposure to BP LMC, using the human bronchial epithelial cell line 16HBE14o-. Epithelial cell monolayers were apically exposed to the major BP allergen Bet v 1, aqueous BP extract or BP-derived LMC. Barrier integrity after the treatments was monitored by measuring transepithelial electrical resistance at regular intervals and by using the xCELLigence Real-Time Cell Analysis system. The polarized release of cytokines 24 h following treatment was measured using a multiplex immunoassay. Epithelial barrier integrity was significantly enhanced upon exposure to BP LMC. Moreover, BP LMC induced the repair of papain-mediated epithelial barrier damage. The apical release of CCL5 and TNF-α was significantly reduced after exposure to BP LMC, while the basolateral release of IL-6 significantly increased. In conclusion, the results of our study demonstrate that BP-derived LMC modify the physical and immunological properties of bronchial epithelial cells and thus regulate airway epithelial barrier responses.

A Review of Subsidence Monitoring Techniques in Offshore Environments.

In view of the ever-increasing global energy demands and the imperative for sustainability in extraction methods, this article surveys subsidence monitoring systems applied to oil and gas fields located in offshore areas. Subsidence is an issue that can harm infrastructure, whether onshore or especially offshore, so it must be carefully monitored to ensure safety and prevent potential environmental damage. A comprehensive review of major monitoring technologies used offshore is still lacking; here, we address this gap by evaluating several techniques, including InSAR, GNSSs, hydrostatic leveling, and fiber optic cables, among others. Their accuracy, applicability, and limitations within offshore operations have also been assessed. Based on an extensive literature review of more than 60 published papers and technical reports, we have found that no single method works best for all settings; instead, a combination of different monitoring approaches is more likely to provide a reliable subsidence assessment. We also present selected case histories to document the results achieved using integrated monitoring studies. With the emerging offshore energy industry, combining GNSSs, InSAR, and other subsidence monitoring technologies offers a pathway to achieving precision in the assessment of offshore infrastructural stability, thus underpinning the sustainability and safety of offshore oil and gas operations. Reliable and comprehensive subsidence monitoring systems are essential for safety, to protect the environment, and ensure the sustainable exploitation of hydrocarbon resources.

The punishment intensity for research misconduct and its related factors: An exploratory study on hospitals in Mainland China.

Previous studies have found that factors such as gender and academic positions do not influence the severity of administrative actions taken by institutions. However, this study provides partly inconsistent evidence. It focuses on incidents of research misconduct in hospitals across Mainland China and explores factors related to punishment using a large cross-sectional dataset (N = 815). Regression analysis revealed a significant correlation between authorship order and the punishment intensity (p < 0.05). Under specific conditions, there was a significant correlation between the professional title (senior) and punishment intensity (p = 0.001), and an interaction between professional title and types of research misbehavior. Further analysis of simple effects showed that, in cases of fabrication and falsification, and combinations of multiple research misbehavior, researchers with senior titles received significantly lighter punishments compared to those with junior, intermediate, and associate senior titles (p < 0.05). The study unveils the potential accountability patterns (collective accountability and tiered punishment) that may be adopted by hospitals in Mainland China, as well as the challenges faced in ensuring fairness, emphasizing the importance of independent investigative bodies for incidents of research misconduct, and advocating for fairness as a priority in governance of research misconduct.

Skoochies and component substances induced testicular damage and impaired sperm functions via increase generation of reactive oxygen species and impairment of the glutathione system in rats.

OBJECTIVE: To study examined the effect of skoochies, an illicit cocktail drink, on testicular and sperm function in male rats. DESIGN: Twenty-five adult male Wistar rats were assigned randomly into five groups (n = 5), as follows: Normal saline (NS group), skoochies (SK group), cannabis sativa (CS group), codeine (CO group), and tramadol (TM group). The cocktail (skoochies) used for this study was formulated using the following composition: codeine (5mg/kg), tramadol (20mg/kg) and cannabis extract (2mg/kg). These doses are as previously reported. Administration was done once daily for twenty-eight (28) days. RESULTS: Skoochies increased reactive oxygen species generation and impaired the antioxidant system resulting in inflammation that eventually damage the testicular tissue. Skoochies causes oxido-inflammatory injury to the testicular tissues resulting in impaired testicular functions. This was evident by the distorted cytoarchitecture, reduced sperm count, motility and impaired testicular DNA integrity. CONCLUSION: Thus, our results infer that Skoochies induced the impairment of testicular and sperm function through the increased generation of reactive oxygen species and impairment of the glutathione system.

Advancements in integrated robotic sensing: A European perspective.

Robotic Non-destructive Testing and Sensing stands at the forefront of technological innovation, offering capabilities in assessing structural integrity, safety, and material quality across diverse industries. This comprehensive review article provides a detailed exploration of the field, focusing on the substantial contributions of European researchers and institutions. The need for non-destructive testing has been a constant in industries that rely on structural integrity, including aerospace, manufacturing, energy, construction, and healthcare. Traditional testing methods, such as radiography, ultrasonic testing, magnetic particle testing, and dye penetrant testing, have been integral for quality control and safety assurance. However, the robotisation of such methods has marked a profound shift, enabling precise, fast, efficient, and repeatable testing while minimising human exposure to hazardous environments. European researchers and institutions have played an instrumental role in driving the evolution of integrated robotic sensing. The historical perspective of the field reveals the pioneering spirit of Europe, as collaborative initiatives led to the development of robotic platforms equipped with advanced sensors and testing techniques. A critical aspect of the European impact on robotic inspection applications lies in developing advanced sensors, innovative robotic platforms, novel robotic path-planning and control approaches and data collection and visualisation tools. These developments continue to influence the global landscape of robotic sensing. European researchers remain at the forefront of current trends and innovations as the field continues to evolve. This review article will delve into these recent advancements, highlighting Europe's pivotal role in pushing the boundaries of technology and application. The implications and applications of robotic sensing reverberate across multiple sectors worldwide. From inspecting critical aerospace components to ensuring the quality of manufactured goods, these technologies underpin safety and quality standards.

Screening of NIAS World Rice Core Collection for Seeds with Long Longevity as Useful Potential Breeding Materials Focusing on the Stability of Embryonic RNAs.

Seed longevity is a crucial trait for the seed industry and genetic resource preservation. To develop excellent cultivars with extended seed lifespans, it is important to understand the mechanism of keeping seed germinability long term and to find useful genetic resources as prospective breeding materials. This study was conducted to identify the best cultivars with a high and stable seed longevity trait in the germplasm of rice (Oryza sativa L.) and to analyze the correlation between seed longevity and embryonic RNA integrity. Seeds from 69 cultivars of the world rice core collection selected by the NIAS in Japan were harvested in different years and subjected to long-term storage or controlled deterioration treatment (CDT). The long-term storage (4 °C, RH under 35%, 10 years) was performed on seeds harvested in 2010 and 2013. The seeds harvested in 2016 and 2019 were used for CDT (36 °C, RH of 80%, 40 days). Seed longevity and embryonic RNA integrity were estimated by a decrease in the germination percentage and RNA integrity number (RIN) after long-term storage or CDT. The RIN value was obtained by the electrophoresis of the total RNA extracted from the seed embryos. Seeds of "Vandaran (indica)", "Tupa 729 (japonica)", and "Badari Dhan (indica)" consistently showed higher seed longevity and embryonic RNA integrity both under long-term storage and CDT conditions regardless of the harvest year. A strong correlation (R2 = 0.93) was observed between the germination percentages and RIN values of the seeds after the long-term storage or CDT among nine cultivars selected based on differences in their seed longevity. The study findings revealed the relationship between rice seed longevity and embryo RNA stability and suggested potential breeding materials including both japonica and indica cultivars for improving rice seed longevity.

Prebiotic Potential of Goji Berry (Lycium barbarum) in Improving Intestinal Integrity and Inflammatory Profiles via Modification of the Gut Microbiota in High-Fat Diet-Fed Rats.

Background: Imbalances in gut microbiota and subsequent destabilization of intestinal barrier equilibrium have been related to the evolution of metabolic disorders. Goji berries (Lycium barbarum; GB) and their fermented counterpart (FGB) have been identified for their prebiotic capacity in managing intestinal barrier functions and inflammatory profiles Consequently, this research was designed to investigate the effects of supplementing GB and FGB on intestinal integrity, inflammation, and changes in the composition of gut microbiota in high-fat (HF)-fed rats. Materials and Methods: Thirty-two male Sprague-Dawley rats (6 weeks old, 8 per group) were divided into four categories based on their weight and provided with either respective diets over a 6-week period: low-fat (LF; 10% of calories from fat), HF (45% of calories from fat), and HF diets supplemented with either GB or FGB at a 2% (w/w). Results: Supplementation of GB and FGB resulted in compositional changes in the gut microbiota, denoted by a distinct abundance of Faecalibacterium prausnitzii with GB and Akkermansia muciniphila species with FGB, which have been linked to ameliorated obesity phenotypes and metabolic parameters. These alterations were correlated with enhancements in gut barrier integrity, thereby protecting against local and systemic inflammation induced by a HF diet. Supplementation with GB and FGB also mitigated lipopolysaccharide-induced inflammation through inhibition of its downstream pathway. Conclusion: These findings indicate that both GB and FGB supplementation can improve gut barrier function and inflammatory profiles in HF-fed rats via modulation of the microbial composition of the gut, supporting the potential application of GB and FGB in improving gut barrier function and managing inflammation amid metabolic challenges.

Evaluation of bone integrity around the acetabular cup using noninvasive laser resonance frequency analysis.

Resonance frequency analysis (RFA) is valuable for assessing implant status. In a previous investigation, acetabular cup fixation was assessed using laser RFA and the pull-down force was predicted in an in vitro setting. While the pull-down force alone is sufficient for initial fixation evaluation, it is desirable to evaluate the bone strength of the foundation for subsequent fixation. Diminished bone quality causes micromotion, migration, and protracted osseointegration, consequently elevating susceptibility to periprosthetic fractures and failure of ingrained trabecular bone. Limited research exists on the evaluation of bone mineral density (BMD) around the cup using RFA. For in vivo application of laser RFA, we implemented the sweep pulse excitation method and engineered an innovative laser RFA device having low laser energy and small dimensions. We focused on a specific frequency range (2500-4500 Hz), where the peak frequency was presumed to be influenced by foundational density. Quantitative computed tomography with a phantom was employed to assess periprosthetic BMD. Correlation between the resonance frequency within the designated range and the density around the cup was evaluated both in the laboratory and in vivo using the novel laser RFA device. The Kruskal-Wallis test showed robust correlations in both experiments (laboratory study: R = 0.728, p < 0.001; in vivo study: R = 0.619, p < 0.001). Our laser RFA system can assess the quality of bone surrounding the cup. Laser RFA holds promise in predicting the risk of loosening and might aid in the decision-making process for additional fixation through screw insertion.

Loss of Sunda clouded leopards and forest integrity drive potential impacts of mesopredator release on vulnerable avifauna.

Amongst the unintended consequences of anthropogenic landscape conversion is declining apex predator abundance linked to loss of forest integrity, which can potentially re-order trophic networks. One such re-ordering, known as mesopredator release, occurs when medium-sized predators, also called mesopredators, rapidly increase in abundance following the decline in apex predator abundance, consequently reducing the abundance of mesopredator prey, notably including terrestrial avifauna. We examine the cascading impacts of declining Sunda clouded leopard abundance, itself consequent upon a reduction in forest integrity, on the mesopredator community of Sabah, Malaysia, to determine whether the phenomenon of mesopredator release is manifest and specifically whether it impacts the terrestrial avifauna community of pheasants and pittas. To explore this trophic interaction, we used a piecewise structural equation model to compare changes in the relative abundance of organisms. Our results suggest that loss of forest integrity may have broad impacts on the community and trigger mesopredator release, the two acting additively in their impact on already vulnerable species of terrestrial avifauna: a result not previously documented in tropical systems and rarely detected even on a global scale. The limiting effect that the Sunda clouded leopard has on the Sunda leopard cat could illuminate the mechanism whereby mesopredator release impacts this system. Both Bulwer's pheasant and pittas appear to be significantly impacted by the increase in Sunda leopard cats, while the great argus pheasant shows similar compelling, although not statistically significant, declines as Sunda leopard cats increase. The inverse relationship between Sunda clouded leopards and Sunda leopard cats suggests that if a mesopredator release exists it could have downstream consequences for some terrestrial avifauna. These results suggest the under-studied interface between mammalian carnivores and avifauna, or more broadly species interactions in general, could offer important conservation tool for holistic ecosystem conservation efforts.

Interaction of sensory processing and balance in adult cochlear implant users.

PUROPSE: The aim of this study was to evaluate the sensory processing skills, quality of life and balance performance in adult cochlear implant users. METHODS: A sample of 40 individuals was studied in two groups; 20 normal hearing individuals (37.84 ± 15.39 years old) and 20 cochlear implant users (35.58 ± 11.22 year old). Balance performance was assessed by Computerized Dynamic Posturography. The Adolescent/Adult Sensory Profile completed by the individual was used to assess sensory processing skills. Finally, quality of life was assessed with the Short Form-36. RESULTS: Among the different sensory processing parameters in the adolescent/adult sensory profile, a significant difference was found between the two groups in the parameters of low registration, sensory sensitivity and sensory avoidance (p < .05). Visual, vestibular and composite scores were found to be lower in cochlear implant users by Computerised Dynamic Posturography assessment (p < .05). When assessing quality of life, emotional well-being and social functioning parameters were found to be lower in cochlear implant users (p < .05). CONCLUSION: Factors such as the inability to reach sufficient auditory stimuli due to hearing loss and the occurrence of vestibular problems after cochlear implant surgery limit the quantity and quality of sensory stimuli from the environment. Individuals with cochlear implants may prefer to live isolated from society because they cannot adequately process incoming sensory stimuli due to hearing/balance problems, and this may negatively affect the quality of life of individuals. Our findings revealed the necessity of multisensory assessment and therapy protocols when rehabilitating individuals with cochlear implants.

Integrity of xylan backbone affects plant responses to drought.

Drought is a major factor affecting crops, thus efforts are needed to increase plant resilience to this abiotic stress. The overlapping signaling pathways between drought and cell wall integrity maintenance responses create a possibility of increasing drought resistance by modifying cell walls. Here, using herbaceous and woody plant model species, Arabidopsis and hybrid aspen, respectively, we investigated how the integrity of xylan in secondary walls affects the responses of plants to drought stress. Plants, in which secondary wall xylan integrity was reduced by expressing fungal GH10 and GH11 xylanases or by affecting genes involved in xylan backbone biosynthesis, were subjected to controlled drought while their physiological responses were continuously monitored by RGB, fluorescence, and/or hyperspectral cameras. For Arabidopsis, this was supplemented with survival test after complete water withdrawal and analyses of stomatal function and stem conductivity. All Arabidopsis xylan-impaired lines showed better survival upon complete watering withdrawal, increased stomatal density and delayed growth inhibition by moderate drought, indicating increased resilience to moderate drought associated with modified xylan integrity. Subtle differences were recorded between xylan biosynthesis mutants (irx9, irx10 and irx14) and xylanase-expressing lines. irx14 was the most drought resistant genotype, and the only genotype with increased lignin content and unaltered xylem conductivity despite its irx phenotype. Rosette growth was more affected by drought in GH11- than in GH10-expressing plants. In aspen, mild downregulation of GT43B and C genes did not affect drought responses and the transgenic plants grew better than the wild-type in drought and well-watered conditions. Both GH10 and GH11 xylanases strongly inhibited stem elongation and root growth in well-watered conditions but growth was less inhibited by drought in GH11-expressing plants than in wild-type. Overall, plants with xylan integrity impairment in secondary walls were less affected than wild-type by moderately reduced water availability but their responses also varied among genotypes and species. Thus, modifying the secondary cell wall integrity can be considered as a potential strategy for developing crops better suited to withstand water scarcity, but more research is needed to address the underlying molecular causes of this variability.