Comparison of content of five flavones in Citrus grandis “Tomentosa” and Citrus grandis with quantitative analysis of muli-components by single marker / 中草药

Objective To establish quantitative analysis of multi-components by single marker (QAMS) to compare five flavones in Citrus grandis “Tomentosa” and C. grandis. Methods Four relative correction factors (RCFs) of neohesperidin, rhoifolin, naringenin, apigenin were established in the HPLC method with the Naringin as internal standard, which were used to determine the content of four flavones in C. grandis “Tomentosa” and C. grandis. Meanwhile, external standard method (ESM) was employed to calculate the content of five flavones. The difference between ESM and QAMS were analyzed to evaluate the accuracy of QAMS. T-test was used to compare five flavones in C. grandis ‘Tomentosa’ and C. grandis. Results RCFs of neohesperidin, rhoifolin, naringenin, apigenin were 0.898, 1.519, 0.313, 0.406, and repeatability was good in different experimental conditions (RSD < 3.0 %). There were no significant differences in the quantitative analysis results of two methods. Content of naringin, rhoifolin and naringenin in two species were significant different. Conclusion The RCFs of neohesperidin, rhoifolin, naringenin, apigenin as reference to naringin are accurate and feasible. Content of flavones in Citrus grandis “Tomentosa” and C. grandis are obvious different. QASM can be applied as a strategy for quality control of C. grandis.

Content Determination of 5 Anthraquinones in Huangzhi Neiyi Capsules by QAMS / 中国中医药信息杂志

Objective To determine the contents of 5 anthraquinones (aloe-emodin, phein, emodin, chrysophanol, physcion)in Huangzhi Neiyi Capsule through a quantitative analysis of multi-component by single-marker(QAMS), To determine the application value of QAMS by comparing with external standard method (ESM). Methods HPLC was performed at 30 ℃ with Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm), methanol-0.4% phosphoric acid (85:15) as mobile phase at the wavelength 440 nm, and flow rate of 1.000 mL/min. The relative correction factors of other anthraquinones were calculated respectively by emodin as the reference. Results The values calculated by QAMS were compared with the values measured by ESM, and no significant difference was showed (RSD<5%). Conclusion For the availability and reliability, QAMS can be applied for quality evaluation of Huangzhi Neiyi Capsules.