Towards sustainable horizons: A comprehensive blueprint for Mars colonization.

This paper thoroughly explores the feasibility, challenges, and proposed solutions for establishing a sustainable human colony on Mars. We quantitatively and qualitatively analyze the Martian environment, highlighting key challenges such as radiation exposure, which astronauts could experience at minimum levels of 0.66 sieverts during a round trip, and the complications arising from Mars' thin atmosphere and extreme temperature variations. Technological advancements are examined, including developing Martian concrete, which utilizes sulfur as a binding agent, and innovative life support strategies like aeroponics and algae bioreactors. The human aspect of colonization is addressed, focusing on long-term space habitation's psychological and physiological impacts. We also present a cost-benefit analysis of in-situ resource utilization versus Earth-based supply missions, emphasizing economic viability with the potential reduction in launch costs through reusable rocket technology. A timeline for the colonization process is suggested, spanning preliminary unmanned missions for resource assessment, followed by short-term manned missions leading to sustainable settlements over several decades. The paper concludes with recommendations for future research, particularly in refining resource utilization techniques and advancing health and life support systems, to solidify the foundation for Mars colonization. This comprehensive assessment aims to guide researchers, policymakers, and stakeholders in planning and executing a strategic and informed approach to making Mars colonization a reality.

The Three-Dimensional Body Center of Mass at the Workplace under Hypogravity.

The center of mass dynamics of the seated posture of humans in a work environment under hypogravity (0 < g < 1) have rarely been investigated, and such research is yet to be carried out. The present study determined the difference in the body system of 32 participants working under simulated 1/6 g (Moon) and 1 g (Earth) for comparison using static and dynamic task measurements. This was based on a markerless motion capture method that analyzed participants' center of mass at the start, middle and end of the task when they began to get fatigued. According to this analysis, there is a positive relationship (p < 0.01) with a positive coefficient of correlation between the downward center of mass body shift along the proximodistal axis and gravity level for males and females. At the same time, the same positive relationship (p < 0.01) between the tilt of the body backward along the anterior-posterior axis and the level of gravity was found only in females. This offers fresh perspectives for comprehending hypogravity in a broader framework regarding its impact on musculoskeletal disorders. It can also improve workplace ergonomics, body stability, equipment design, and biomechanics.

Hypogravity modeling of upper extremities: an investigation of manual handling in the workplace.

Experiments on the lower limbs are the only approaches being used to study how hypogravity (HG) (0 < g < 1, e.g., Moon: 1/6 g, Mars: 3/8 g) affects human movement. The goal of this study was to expand this field experimentally by investigating the effect of HG on the upper extremities during one-handed manual handling tasks in a sitting posture: static weight holding with an outstretched arm, and slow repetitive weight lifting and lowering motions. The hypothesis was that while completing static and dynamic tasks with elements of repetition in HG, the upper body's tilt (angle regarding the vertical axis) would change differently from Earth's gravity. Specifically, upper arm and spine angles, joint torques, and forces were investigated. Twenty-four healthy participants aged 33.6 ± 8.2 years were involved in the trial. Joint angles were examined using vision-based 3D motion analysis. According to this investigation, there is a correlation between a body tilting backward and a gravity level reduction (p < 0.01). Thus, HG causes postural deviation, and this shows that workplace design must be adapted according to the level of gravity to promote comfortable and balanced body alignment, minimizing stress on muscles and joints. To lower the risk of musculoskeletal disorders (MSDs), enhance overall performance, and increase job satisfaction, proper support systems and restrictions for sitting positions should be taken into account, concerning different levels of gravity.

Characterizing Bodyweight-Supported Treadmill Walking on Land and Underwater Using Foot-Worn Inertial Measurement Units and Machine Learning for Gait Event Detection.

Gait rehabilitation commonly relies on bodyweight unloading mechanisms, such as overhead mechanical support and underwater buoyancy. Lightweight and wireless inertial measurement unit (IMU) sensors provide a cost-effective tool for quantifying body segment motions without the need for video recordings or ground reaction force measures. Identifying the instant when the foot contacts and leaves the ground from IMU data can be challenging, often requiring scrupulous parameter selection and researcher supervision. We aimed to assess the use of machine learning methods for gait event detection based on features from foot segment rotational velocity using foot-worn IMU sensors during bodyweight-supported treadmill walking on land and underwater. Twelve healthy subjects completed on-land treadmill walking with overhead mechanical bodyweight support, and three subjects completed underwater treadmill walking. We placed IMU sensors on the foot and recorded motion capture and ground reaction force data on land and recorded IMU sensor data from wireless foot pressure insoles underwater. To detect gait events based on IMU data features, we used random forest machine learning classification. We achieved high gait event detection accuracy (95-96%) during on-land bodyweight-supported treadmill walking across a range of gait speeds and bodyweight support levels. Due to biomechanical changes during underwater treadmill walking compared to on land, accurate underwater gait event detection required specific underwater training data. Using single-axis IMU data and machine learning classification, we were able to effectively identify gait events during bodyweight-supported treadmill walking on land and underwater. Robust and automated gait event detection methods can enable advances in gait rehabilitation.

Reduced gravity effects on gait coordinative structures.

Humans have stepped on the Lunar surface for less than 80 h of Extravehicular Activity, providing a narrow understanding of Lunar gait patterns. NASA's Human-crewed Artemis missions are quickly approaching; understanding how fractional gravity affects gait patterns will be critical for the Moon's and Mars' long-term habitation. This study examined gait patterns under 1.0 g (Earth), simulated 0.38 g (Martian), and 0.17 g (Lunar). Participants walked and ran on a treadmill supported by ARGOS (Active Response Gravity Offload System), simulating fractional gravity. Vicon motion capture data and principal component analysis software were used to capture and quantify coordinated gait structures. There were found to be significant differences (p < 0.05) in the coordinative gait structures for ambulation between fractional gravity conditions. Additionally, there were significantly higher asymmetric gait components for Lunar conditions. Finally, a skipping coordinative structure was identified within Lunar and Martian running.

Computational analysis of Darcy-Forchheimer relation, reduced gravity, and external applied magnetic field influence on radiative fluid flow and heat transfer past a sphere: Finite difference method.

The study of radiation, Darcy-Forchheimer relation, and reduced gravity, effects on magnetohydrodynamic flow across a solid sphere immersed in porous material, is the focus of the current work. Coupled and nonlinear partial differential governing equations, are established to model the studied configuration. By using appropriate scaling variables, the resultant set of governing equations is converted to its dimensionless form. Based on these established equations, a numerical algorithm is written based on the finite element approach to solve the considered problem. A verification of the validity of the proposed model is done by comparing with already published results. Furthermore, to check the precision of solutions, a grid independence test has been accomplished. The unknown variables, such as fluid velocity and temperature, and their gradients are evaluated. This investigation's main objective is to demonstrate how the Darcy-Forchheimer law and reduced gravity due to density difference affect the natural convective heat transfer across a solid sphere immersed in a porous medium. Results show that the flow intensity decreases with the magnetic field parameter, local inertial coefficient, Prandtl number, and porosity parameter and becomes more important by increasing the reduced gravity and radiation parameters. In addition, the temperature increases with the inertial coefficient, porosity parameter, Prandtl number, radiation parameter, and magnetic field parameter and get declined with the reduced gravity parameter.

Development and Validation of an Active Muscle Simplified Finite Element Human Body Model in a Standing Posture.

Active muscles play an important role in postural stabilization, and muscle-induced joint stiffening can alter the kinematic response of the human body, particularly that of the lower extremities, under dynamic loading conditions. There are few full-body human body finite element models with active muscles in a standing posture. Thus, the objective of this study was to develop and validate the M50-PS+Active model, an average-male simplified human body model in a standing posture with active musculature. The M50-PS+Active model was developed by incorporating 116 skeletal muscles, as one-dimensional beam elements with a Hill-type material model and closed-loop Proportional Integral Derivative (PID) controller muscle activation strategy, into the Global Human Body Models Consortium (GHBMC) simplified pedestrian model M50-PS. The M50-PS+Active model was first validated in a gravity standing test, showing the effectiveness of the active muscles in maintaining a standing posture under gravitational loading. The knee kinematics of the model were compared against volunteer kinematics in unsuited and suited step-down tests from NASA's active response gravity offload system (ARGOS) laboratory. The M50-PS+Active model showed good biofidelity with volunteer kinematics with an overall CORA score of 0.80, as compared to 0.64 (fair) in the passive M50-PS model. The M50-PS+Active model will serve as a useful tool to study the biomechanics of the human body in vehicle-pedestrian accidents, public transportation braking, and space missions piloted in a standing posture.

An Empirical and Subjective Model of Upper Extremity Fatigue Under Hypogravity.

In the context of extra-terrestrial missions, the effects of hypogravity (0 < G < 1) on the human body can reduce the well-being of the crew, cause musculoskeletal problems and affect their ability to perform tasks, especially during long-term missions. To date, studies of the effects of hypogravity on human movement are limited to experiments on the lower limbs. Here, we extend the knowledge base to the upper limbs, by conducting experiments to evaluate the effect of hypogravity on upper limb physical fatigue and mental workload in participants. Our hypothesis was that hypogravity would both increase participant productivity, by reducing overall physical fatigue expressed in Endurance Time, and reduce mental workload. Task Intensity-Endurance time curves are developed especially in seated positions, while performing static, dynamic, repetitive tasks. This experiment involved 32 healthy participants without chronic problems of the musculoskeletal system aged 33.59 ± 8.16 years. Using the collected data, fatigue models were constructed for tasks of varying Intensity. In addition, all participants completed the NASA - Task Load Index subjective mental workload assessment, which revealed the level of subjective workload when executing different tasks. We found two trends in the empirical fatigue models associated with the difference between the strength capabilities of males and females. The first is a significant positive (p = 0.002) relation between Endurance time and gravity level (⅙ G Moon, ⅓ G Mars, 1G) with negative coefficient for males and females for a static task. And there is marginal relation (p < 0.1) between overall mental workload and gravity level with a positive coefficient for males and females for the same task. The same trend was observed for dynamic and repetitive tasks. We concluded that the Task Intensity-Endurance Time model, adapted to hypogravity in combination with subjective mental assessment, is useful to human fatigue investigation. The combination of these methods used for ergonomic analysis and digital human modeling, could improve worker productivity. Finally, this study may help prepare astronauts for long-term missions on the Moon and Mars and improve our understanding of how we can prevent musculoskeletal disorders caused by hazardous manual handling under such extreme environments.

Economical and preferred walking speed using body weight support apparatus with a spring-like characteristics.

BACKGROUND: A specific walking speed minimizing the U-shaped relationship between energy cost of transport per unit distance (CoT) and speed is called economical speed (ES). To investigate the effects of reduced body weight on the ES, we installed a body weight support (BWS) apparatus with a spring-like characteristics. We also examined whether the 'calculated' ES was equivalent to the 'preferred' walking speed (PWS) with 30% BWS. METHODS: We measured oxygen uptake and carbon dioxide output to calculate CoT values at seven treadmill walking speeds (0.67-2.00 m s- 1) in 40 healthy young males under normal walking (NW) and BWS. The PWS was determined under both conditions on a different day. RESULTS: A spring-like behavior of our BWS apparatus reduced the CoT values at 1.56, 1.78, and 2.00 m s- 1. The ES with BWS (1.61 ± 0.11 m s- 1) was faster than NW condition (1.39 ± 0.06 m s- 1). A Bland-Altman analysis indicated that there were no systematic biases between ES and PWS in both conditions. CONCLUSIONS: The use of BWS apparatus with a spring-like behavior reduced the CoT values at faster walking speeds, resulting in the faster ES with 30% BWS compared to NW. Since the ES was equivalent to the PWS in both conditions, the PWS could be mainly determined by the metabolic minimization in healthy young males. This result also derives that the PWS can be a substitutable index of the individual ES in these populations.

Use of Pressure-Measuring Insoles to Characterize Gait Parameters in Simulated Reduced-Gravity Conditions.

Prior researchers have observed the effect of simulated reduced-gravity exercise. However, the extent to which lower-body positive-pressure treadmill (LBPPT) walking alters kinematic gait characteristics is not well understood. The purpose of the study was to investigate the effect of LBPPT walking on selected gait parameters in simulated reduced-gravity conditions. Twenty-nine college-aged volunteers participated in this cross-sectional study. Participants wore pressure-measuring insoles (Medilogic GmBH, Schönefeld, Germany) and completed three 3.5-min walking trials on the LBPPT (AlterG, Inc., Fremont, CA, USA) at 100% (normal gravity) as well as reduced-gravity conditions of 40% and 20% body weight (BW). The resulting insole data were analyzed to calculate center of pressure (COP) variables: COP path length and width and stance time. The results showed that 100% BW condition was significantly different from both the 40% and 20% BW conditions, p < 0.05. There were no significant differences observed between the 40% and 20% BW conditions for COP path length and width. Conversely, stance time significantly differed between the 40% and 20% BW conditions. The findings of this study may prove beneficial for clinicians as they develop rehabilitation strategies to effectively unload the individual's body weight to perform safe exercises.

Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition.

Polyhydramnios is a condition related to an excessive accumulation of amniotic fluid in the third trimester of pregnancy and it can be acute and chronic depending on the duration. Published data suggest that during muscle development, in the stage of late histochemical differentiation decreased mechanical loading cause decreased expression of myosin heavy chain (MHC) type 1 leading to slow-to-fast transition. In the case of chronic polyhydramnios, histochemical muscle differentiation could be affected as a consequence of permanent decreased physical loading. Most affected would be muscles which are the most active i.e., spine extensor muscles and muscles of legs. Long-lasting decreased mechanical loading on muscle should cause decreased expression of MHC type 1 leading to slow-to-fast transition, decreased number of muscle fiber type I especially in extensor muscles of spine and legs. Additionally, because MHC type 1 is present in all skeletal muscles it could lead to various degrees of hypotrophy depending on constituting a percentage of MHC type 1 in affected muscles. These changes in the case of preexisting muscle disorders have the potential to deteriorate the muscle condition additionally. Given these facts, idiopathic chronic polyhydramnios is a rare opportunity to study the influence of reduced physical loading on muscle development in the human fetus. Also, it could be a medical entity to examine the influence of micro- and hypogravity conditions on the development of the fetal muscular system during the last trimester of gestation.

The metabolic power required to support body weight and accelerate body mass changes during walking on uphill and downhill slopes.

The metabolic cost of walking is due to muscle force generated to support body weight (BW), external work performed to redirect and accelerate the center of mass (CoM), and internal work performed to swing the limbs and maintain balance. We hypothesized that BW support would incur a greater and lower percentage of Net Metabolic Power (NMP) for uphill and downhill slopes, respectively, compared to level-ground walking. Additionally, we hypothesized that mass redirection would incur a greater and lower percentage of NMP for uphill and downhill slopes, respectively compared to level-ground walking. 10 subjects walked at 1.25 m/s on 0°, ±3°, and ±6° slopes with reduced/added weight and added mass while we measured metabolic rates. We calculated NMP per Newton of reduced BW at each slope and found that BW support required 58% and 64% of the NMP to walk at +3° and +6°, respectively, both greater than the 15% required for level-ground walking (p < 0.025). We calculated NMP per kg of added mass at each slope and found that mass redirection required 19% and 23% of the NMP to walk at +3° and +6°, respectively, both lower than the 35% required for level-ground walking (p < 0.025). We found no significant differences in the percentage of NMP for BW support or mass redirection during downhill compared to level ground walking (p > 0.05). Our findings elucidate that the percentage of NMP attributed to BW support and mass redirection is different for sloped compared to level-ground walking. These results inform biomimetic assistive device designs aimed at reducing metabolic cost.

Biomechanical Analysis of Lower Limbs during Walking under Simulated Reduced Gravity / 医用生物力学

Objective To investigate the influence of gravity levels on lower limb motions during human walking. Methods A suspended microgravity simulation system was designed for the experiment. Kinetic parameters from lower limb joints of twelve volunteers during walking were measured by the motion capture system and 3D force plate under simulated Mars gravity (1/3 G), lunar gravity (1/6 G) and earth gravity (1 G). Results Under simulated Mars and lunar gravity, the ranges of motion (ROMs) of hip and knee joints in sagittal plane significantly decreased (P＜0.01) while the ROMs of ankle joints obviously increased (P＜0.05). The ROMs of hip, knee and ankle joints in sagittal plane under earth gravity were 45.2°, 67.7°, 32.5°, respectively, while the ROMs of hip, knee and ankle joints under lunar gravity were 25.1°, 50.8°, 42.4°, respectively. In addition, the force and torque of lower limb joints in sagittal plane under lower gravity also decreased significantly (P＜0.01). Conclusions The results obtained by this study were almost the same as the research findings obtained by using treadmill. Astronauts can use the treadmill and microgravity simulation system for walking exercises under lunar gravity on the earth.

Comparison of Microgravity Analogs to Spaceflight in Studies of Plant Growth and Development.

Life on Earth has evolved under the influence of gravity. This force has played an important role in shaping development and morphology from the molecular level to the whole organism. Although aquatic life experiences reduced gravity effects, land plants have evolved under a 1-g environment. Understanding gravitational effects requires changing the magnitude of this force. One method of eliminating gravity''s influence is to enter into a free-fall orbit around the planet, thereby achieving a balance between centripetal force of gravity and the centrifugal force of the moving object. This balance is often mistakenly referred to as microgravity, but is best described as weightlessness. In addition to actually compensating gravity, instruments such as clinostats, random-positioning machines (RPM), and magnetic levitation devices have been used to eliminate effects of constant gravity on plant growth and development. However, these platforms do not reduce gravity but constantly change its direction. Despite these fundamental differences, there are few studies that have investigated the comparability between these platforms and weightlessness. Here, we provide a review of the strengths and weaknesses of these analogs for the study of plant growth and development compared to spaceflight experiments. We also consider reduced or partial gravity effects via spaceflight and analog methods. While these analogs are useful, the fidelity of the results relative to spaceflight depends on biological parameters and environmental conditions that cannot be simulated in ground-based studies.

Stumbling Reactions in Partial Gravity - Neuromechanics of Compensatory Postural Responses and Inter-Limb Coordination During Perturbation of Human Stance.

Spontaneous changes in gravity play a significant role in interplanetary space missions. To preserve the astronauts' capability to execute mission-critical tasks and reduce the risk of injury in transit and on planetary surfaces, a comprehensive understanding of the neuromuscular control of postural responses after balance deterioration in hypo- or hyper-gravity conditions is essential. Therefore, this study aimed to evaluate the effect of acute gravitational variation on postural adjustments in response to perturbations. Gravitational changes were induced using parabolic flight. Postural set was manipulated by randomly providing unilateral left, bilateral or split perturbations which require balance corrections to restore postural stability. In six subjects, postural reactions were recorded after anterior and posterior surface perturbations for progressively increased gravitational conditions spanning from 0.25 to 1.75 g. Ankle and knee joint kinematics and electromyograms (EMG) of eight leg muscles were recorded prior (PRE) and after perturbation onset. Muscle activation onset latencies and amplitudes in the short-, medium-, and long-latency responses (SLR, MLR, LLR) were assessed. Results demonstrate an increased muscle activity (p < 0.05) and co-contraction in the lower extremities (p < 0.05) prior to perturbation in hypo- and hyper-gravity. After perturbation, reduced muscle onset latencies (p < 0.05) and increased muscle activations in the MLR and LLR (p < 0.05), concomitant with an increased co-contraction in the SLR, were manifested with a progressive rise in gravity. Ankle and knee joint deflections remained unaffected, whereas angular velocities increased (p < 0.05) with increasing gravitation. Effects were more pronounced in bi- compared to unilateral or split perturbations (p < 0.05). Neuro-mechanical adaptations to gravity were more distinct and muscle onset latencies were shorter in the displaced compared to the non-displaced leg. In conclusion, the timing and magnitude of postural reflexes involved in stabilization of bipedal stance are gravity-dependent. The approximately linear relationship between gravity and impulse-directed EMG amplitudes or muscle onset latencies after perturbation indicates that the central nervous system correctly predicts the level of gravity. Moreover, it accurately governs contractions in the antigravity musculature to counterbalance the gravitational pull and to regain upright posture after its disturbance. Importantly, unilateral perturbations evoked fast reflex responses in the synergistic muscles of the non-displaced contralateral leg suggesting a synchronized inter-limb coordination mediated by spinal circuitries.

Reducing gravity takes the bounce out of running.

In gravity below Earth-normal, a person should be able to take higher leaps in running. We asked 10 subjects to run on a treadmill in five levels of simulated reduced gravity and optically tracked centre-of-mass kinematics. Subjects consistently reduced ballistic height compared with running in normal gravity. We explain this trend by considering the vertical take-off velocity (defined as maximum vertical velocity). Energetically optimal gaits should balance the energetic costs of ground-contact collisions (favouring lower take-off velocity), and step frequency penalties such as leg swing work (favouring higher take-off velocity, but less so in reduced gravity). Measured vertical take-off velocity scaled with the square root of gravitational acceleration, following energetic optimality predictions and explaining why ballistic height decreases in lower gravity. The success of work-based costs in predicting this behaviour challenges the notion that gait adaptation in reduced gravity results from an unloading of the stance phase. Only the relationship between take-off velocity and swing cost changes in reduced gravity; the energetic cost of the down-to-up transition for a given vertical take-off velocity does not change with gravity. Because lower gravity allows an elongated swing phase for a given take-off velocity, the motor control system can relax the vertical momentum change in the stance phase, thus reducing ballistic height, without great energetic penalty to leg swing work. Although it may seem counterintuitive, using less 'bouncy' gaits in reduced gravity is a strategy to reduce energetic costs, to which humans seem extremely sensitive.

A novel blue-light phototropic response is revealed in roots of Arabidopsis thaliana in microgravity.

MAIN CONCLUSION: Blue-light positive phototropism in roots is masked by gravity and revealed in conditions of microgravity. In addition, the magnitude of red-light positive phototropic curvature is correlated to the magnitude of gravity. Due to their sessile nature, plants utilize environmental cues to grow and respond to their surroundings. Two of these cues, light and gravity, play a substantial role in plant orientation and directed growth movements (tropisms). However, very little is currently known about the interaction between light- (phototropic) and gravity (gravitropic)-mediated growth responses. Utilizing the European Modular Cultivation System on board the International Space Station, we investigated the interaction between phototropic and gravitropic responses in three Arabidopsis thaliana genotypes, Landsberg wild type, as well as mutants of phytochrome A and phytochrome B. Onboard centrifuges were used to create a fractional gravity gradient ranging from reduced gravity up to 1g. A novel positive blue-light phototropic response of roots was observed during conditions of microgravity, and this response was attenuated at 0.1g. In addition, a red-light pretreatment of plants enhanced the magnitude of positive phototropic curvature of roots in response to blue illumination. In addition, a positive phototropic response of roots was observed when exposed to red light, and a decrease in response was gradual and correlated with the increase in gravity. The positive red-light phototropic curvature of hypocotyls when exposed to red light was also confirmed. Both red-light and blue-light phototropic responses were also shown to be affected by directional light intensity. To our knowledge, this is the first characterization of a positive blue-light phototropic response in Arabidopsis roots, as well as the first description of the relationship between these phototropic responses in fractional or reduced gravities.

Plant biology in reduced gravity on the Moon and Mars.

While there have been numerous studies on the effects of microgravity on plant biology since the beginning of the Space Age, our knowledge of the effects of reduced gravity (less than the Earth nominal 1 g) on plant physiology and development is very limited. Since international space agencies have cited manned exploration of Moon/Mars as long-term goals, it is important to understand plant biology at the lunar (0.17 g) and Martian levels of gravity (0.38 g), as plants are likely to be part of bioregenerative life-support systems on these missions. First, the methods to obtain microgravity and reduced gravity such as drop towers, parabolic flights, sounding rockets and orbiting spacecraft are reviewed. Studies on gravitaxis and gravitropism in algae have suggested that the threshold level of gravity sensing is around 0.3 g or less. Recent experiments on the International Space Station (ISS) showed attenuation of phototropism in higher plants occurs at levels ranging from 0.l g to 0.3 g. Taken together, these studies suggest that the reduced gravity level on Mars of 0.38 g may be enough so that the gravity level per se would not be a major problem for plant development. Studies that have directly considered the impact of reduced gravity and microgravity on bioregenerative life-support systems have identified important biophysical changes in the reduced gravity environments that impact the design of these systems. The author suggests that the current ISS laboratory facilities with on-board centrifuges should be used as a test bed in which to explore the effects of reduced gravity on plant biology, including those factors that are directly related to developing life-support systems necessary for Moon and Mars exploration.

Cardiogenic mixing increases aerosol deposition in the human lung in the absence of gravity.

RATIONALE: Exposure to extraterrestrial dusts is an almost inevitable consequence of any proposed planetary exploration. Previous studies in humans showed reduced deposition in low-gravity compared with normal gravity (1G). However, the reduced sedimentation means that fewer particles deposit in the airways, increasing the number of particles transported to the lung periphery where they eventually deposit albeit at a smaller rate than in 1G. In this study, we determined the role that gravity and other mechanisms such as cardiogenic mixing play in peripheral lung deposition during breath holds. METHODS: Eight healthy subjects inhaled boluses of 0.5 µm-diameter particles to penetration volumes (Vp) of 300 and 1200ml that were followed by breath holds of up to 10 sec. Tests were performed in 1G and during short periods of microgravity (µG) aboard the NASA Microgravity Research Aircraft. Aerosol deposition and dispersion were calculated from these data. RESULTS: Results show that, for both Vp, deposition in 1G was significantly higher than in µG. In contrast, while dispersion was significantly higher in 1G compared to µG at Vp=1200ml, there was no significant gravitational effect on dispersion at Vp=300ml. Finally, for each G level and Vp, deposition and dispersion significantly increased with increasing breath-hold time. CONCLUSION: The most important finding of this study is that, even in the absence of gravity, aerosol deposition in the lung periphery increased with increasing residence time. Because the particles used in this study were too large to be significantly affected by Brownian diffusion, the increase in deposition is likely due to cardiogenic motion effects.