ABSTRACT
Background: Type 2 diabetes mellitus (T2DM) is growing illnesses associated with metabolic dysregulation such as obesity affecting a large population become leading causes of death worldwide. Fibronectin type III domain-containing protein 5 (FNDC-5) and selectin-E were suggested to have effects on metabolism and diabetes, therefore present study aimed to evaluate the clinical importance of FNDC-5 and selectin-E among the T2DM patients with and without obesity. Methods: Study included cohort of 200 T2DM patients with and without obesity. We evaluated FNDC-5, selectin-E mRNA expression as well as vitamin-D, and vitamin-B12 levels in among the T2DM patients with and without obesity. Results: Study observed significant difference in biochemical parameters included in study. T2DM patients with obesity had significantly higher fasting blood glucose levels (p<0.0001) and HbA1c (glycated hemoglobin) (p<0.0001) compared to those T2DM patients without obesity. T2DM patients with obesity also had higher systolic blood pressure (p=0.001), LDL (low density lipoprotein) (p=0.02), TG (triglycerides) (p=0.02) and cholesterol (p=0.01) compared to T2DM patients without obesity. The mRNA expression of FNDC-5 (p<0.0001) was lower in T2DM patients with obesity compared to T2DM patients without obesity. It was observed that the T2DM patients with vitamin-D deficiency had significantly lower FNDC-5 mRNA expression (p=0.03) when compared with those with sufficient vitamin-D level. T2DM patients with clinically normal vitamin-B12 level expressed 0.60 fold FNDC-5 mRNA expression while B12 deficient T2DM patients had 0.28 fold FNDC-5 mRNA expression (p=0.005). No as such significant association was was observed with selectin-E. A negative correlation of FNDC-5 mRNA expression with Post prandial glucose (mg/dl) (p=0.04) and TG (mg/dl) (p=0.02) was observed. Conclusion: FNDC-5 down regulation was observed with T2DM with obesity, vitamin-D and vitamin-B12 deficiency suggesting obesity, vitamin-D and vitamin-B12 deficiency could be the factor for FNDC-5 down-regulation leading to worseness or progression of disease. We suggest that FNDC-5 down-regulation could be used as an indicator for T2DM worseness and development of other associated complications.
ABSTRACT
We investigated the expression of platelet derived growth factor alpha (PDGFA); its receptor, PDGFRA; integrin subunit alpha V; and selectin E in cutaneous fibrosarcomas in dogs. Ten cases of canine fibrosarcomas were obtained from the archive of the Department of Pathology, Ondokuz Mayis University, Samsun. Tissue sections were cut and stained with hematoxylin and eosin, Alcian blue-periodic acid Schiff, Masson's trichrome, and also immunostained. Eight tumors classified as spontaneous fibrosarcomas exhibited interwoven bundles of spindle shaped cells with oval to plump nuclei and scant cytoplasm, while two tumors exhibited features of injection site fibrosarcoma with peripheral infiltration of mononuclear cells and intratumor necrosis. We found that neoplastic cells from all cases exhibited cytoplasmic expression of PDGFA, and cytoplasmic and nuclear staining for PDGFRA. Integrin subunit alpha V immunostaining was observed in all cases, while selectin E expression was observed in vascular endothelial cells and neoplastic cells. A significant positive correlation was found between the expression of PDGFA and integrin subunit alpha V. Our findings indicate that PDGFA, PDGFRA, integrin subunit alpha V and selectin E are expressed strongly in canine cutaneous fibrosarcomas and may contribute to tumor progression.
Subject(s)
Endothelial Cells , Fibrosarcoma , Animals , Dog Diseases , Dogs , Fibrosarcoma/genetics , Fibrosarcoma/veterinary , Integrins , Platelet-Derived Growth Factor/genetics , SelectinsABSTRACT
Objective: To investigate the level of cell adhesion molecule E-selectin in colorectal neoplasm and its relations with metastasis in the blood line. Methods:E-selectin expression in colorectal carcinoma and normal colorectal tissue were detected with immunohisochemical technique. The change of shift ability of human colorectal neoplasm cell line was analyzed. Results: The expressing rate of E-selectin was 57.5%,obviously higher than 6.67% in the normal colorectal tissue(P
ABSTRACT
Objective To study the histocompatibility of tissue engineered skin with the observation of the effects of selectins E and P on the immunological rejection after skin allograft in rats. Methods Tissue engineered skin was prepared as follows: The materials obtained from the neonatal SD rats were cultured and then grafted onto the adult Wistar rats. The expression of selectins E and P in the grafted skin was determined with immunohistochemical staining. Results The expression of selectins E and P was significantly higher in the rats with allograft than in the rats with the grafts of tissue-engineered material. Conclusion Selectins E and P play an important role in the immunological rejection after allograft of skin but the tissue-engineered skin graft possesses favorable histocompatibility and shows no obvious immunological rejection.
