ABSTRACT
Background: Discovering biological markers is essential for understanding and treating mental disorders. Despite the limitations of current non-invasive methods, neural progenitor cells from the olfactory epithelium (hNPCs-OE) have been emphasized as potential biomarker sources. This study measured soluble factors in these cells in Major Depressive Disorder (MDD), Borderline Personality Disorder (BPD), and healthy controls (HC). Methods: We assessed thirty-five participants divided into MDD (n=14), BPD (n=14), and HC (n=7). MDD was assessed using the Hamilton Depression Rating Scale. BPD was evaluated using the DSM-5 criteria and the Structured Clinical Interview for Personality Disorders. We isolated hNPCs-OE, collected intracellular proteins and conditioned medium, and quantified markers and soluble factors, including Interleukin-6, interleukin-8, and others. Analysis was conducted using one-way ANOVA or Kruskal-Wallis test and linear regression. Results: We found that hNPCs-OE of MDD and BPD decreased Sox2 and laminin receptor-67 kDa levels. MASH-1 decreased in BPD, while tubulin beta-III decreased in MDD compared to controls and BPD. Also, we found significant differences in IL-6, IL-8, MCP-1, and thrombospondin-1 levels between controls and MDD, or BPD, but not between MDD and BPD. Conclusions: Altered protein markers are evident in the nhNPCs-OE in MDD and BPD patients. These cells also secrete higher concentrations of inflammatory cytokines than HC cells. The results suggest the potential utility of hNPCs-OE as an in vitro model for researching biological protein markers in psychiatric disorders. However, more extensive validation studies are needed to confirm their effectiveness and specificity in neuropsychiatric disorders.
ABSTRACT
Skeletal muscle tissue has the critical function of mechanical support protecting the body. In addition, its functions are strongly influenced by the balanced synthesis and degradation processes of structural and regulatory proteins. The inhibition of protein synthesis and/or the activation of catabolism generally determines a pathological state or condition called muscle atrophy, a reduction in muscle mass that results in partial or total loss of function. It has been established that many pathophysiological conditions can cause a decrease in muscle mass. Skeletal muscle innervation involves stable and functional neural interactions with muscles via neuromuscular junctions and is essential for maintaining normal muscle structure and function. Loss of motor innervation induces rapid skeletal muscle fiber degeneration with activation of atrophy-related signaling and subsequent disassembly of sarcomeres, altering normal muscle function. After denervation, an inflammation stage is characterized by the increased expression of pro-inflammatory cytokines that determine muscle atrophy. In this review, we highlighted the impact of some soluble factors on the development of muscle atrophy by denervation.
Subject(s)
Muscle Denervation , Muscular Atrophy , Humans , Muscle Denervation/adverse effects , Muscular Atrophy/genetics , Muscular Atrophy/metabolism , Muscle, Skeletal/metabolism , Signal Transduction , Cytokines/metabolismABSTRACT
Immunometabolism research is uncovering the relationship between metabolic features and immune cell functions in physiological and pathological conditions. Normal pregnancy entails a fine immune and metabolic regulation of the maternal-fetal interaction to assist the energetic demands of the fetus with immune homeostasis maintenance. Here, we determined the immunometabolic status of monocytes of pregnant women compared with nonpregnant controls and its impact on monocyte anti-inflammatory functions such as efferocytosis. Monocytes from pregnant women (16-20 wk) and nonpregnant age-matched controls were studied. Single cell-based metabolic assays using freshly isolated monocytes from both groups were carried out in parallel with functional assays ex vivo to evaluate monocyte efferocytic capacity. On the other hand, various in vitro metabolic assays with human monocytes or monocyte-derived macrophages were designed to explore the effect of trophoblast cells in the profiles observed. We found that pregnancy alters monocyte metabolism and function. An increased glucose dependency and enhanced efferocytosis were detected in monocytes from pregnant women at resting states, compared with nonpregnant controls. Furthermore, monocytes display a reduced glycolytic response when stimulated with lipopolysaccharide (LPS). The metabolic profiling of monocytes at this stage of pregnancy was comparable with the immunometabolic phenotypes of human monocytes treated in vitro with human first trimester trophoblast cell conditioned media. These findings suggest that immunometabolic mechanisms are involved in the functional shaping of monocytes during pregnancy with a contribution of trophoblast cells. Results provide new clues for future hypotheses regarding pregnancies complicated by metabolic disorders.NEW & NOTEWORTHY Immunometabolism stands as a novel perspective to understand the complex regulation of the immune response and to provide small molecule-based therapies. By applying this approach to study monocytes during pregnancy, we found that these cells have a unique activation pattern. They rely more on glycolysis and show increased efferocytosis/IL-10 production, but they do not have the typical proinflammatory responses. We also present evidence that trophoblast cells can shape monocytes into this distinct immunometabolic profile.
Subject(s)
Monocytes , Trophoblasts , Pregnancy , Humans , Female , Monocytes/metabolism , Trophoblasts/metabolism , Macrophages/metabolism , Pregnancy Trimester, FirstABSTRACT
Adipocytes are the main stromal cells in the mammary microenvironment, and crosstalk between adipocytes and breast cancer cells may play a critical and important role in cancer maintenance and progression. Tumorinduced differentiation to beige/brown adipose tissue is an important contribution to the hypermetabolic state of breast cancer. However, the effect of epithelial cellbeige adipocyte communication on tumor progression remains unclear. To contribute to the understanding of this phenomenon, we characterized components present in conditioned media (CM) from beige adipocytes (BAs) or white adipocytes (WAs), and evaluated the effects of BA and WACM on both adhesion and migration of tumor (LM3, 4T1 and MC4L1) and nontumor (NMuMG) mouse mammary epithelial cell lines. Additionally, we analyzed the expression of ObR, CD44, vimentin, MMP9, MCT1 and LDH in tumor and nontumor mouse mammary epithelial cell lines incubated with BACM, WACM or CtrolCM (control conditioned media). 3T3L1 preadipocytes differentiated into beige adipocytes upon PPARγ activation (rosiglitazone) displaying characteristics that morphologically resembled brown/beige adipocytes. Levels of UCP1, CIDEA, GLUT4, leptin, MCT4 and FABP4 were increased, while adiponectin, caveolin 1 and perilipin 1 levels were decreased in BAs with respect to WAs. Tumor cell lines revealed lower cell adhesion and increased cell migration after incubation with BA and WACM vs. CtrolCM. ObR and MMP9 in MC4L1 cells were significantly increased after incubation with BACM vs. WA and CtrolCM. In addition, MC4L1 and LM3 cells significantly increased their migration in the presence of BAs, suggesting that new signals originating from the crosstalk between BAs and tumor cells, could be responsible for this change. Our results indicate that beige adipocytes are able to regulate the behavior of both tumor and nontumor mouse mammary epithelial cells, favoring tumor progression.
Subject(s)
Adipocytes, Beige/metabolism , Breast Neoplasms/pathology , Mammary Neoplasms, Experimental/pathology , 3T3-L1 Cells , Adipocytes, Beige/drug effects , Adipocytes, White/drug effects , Adipocytes, White/metabolism , Animals , Cell Adhesion/drug effects , Cell Differentiation/drug effects , Cell Line, Tumor , Cell Movement/drug effects , Culture Media, Conditioned/metabolism , Disease Progression , Female , Humans , Mammary Glands, Animal , Mice , PPAR gamma/agonists , PPAR gamma/metabolism , Rosiglitazone/pharmacology , Tumor Microenvironment/drug effectsABSTRACT
Neurogenesis plays a significant role during adulthood, and the observation that neural stem cells reside in the central nervous system and the olfactory epithelium has attracted attention due to their importance in neuronal regeneration. In addition, soluble factors (SFs) release by neural stem cells may modulate the neurogenic process. Thus, in this study, we identified the SFs released by olfactory human neural stem/progenitor cells (hNS/PCs-OE). These cells express Ki67, nestin, and ßIII-tubulin, indicating their neural lineage. The hNS/PCs-OE also express PSD95 and tau proteins during proliferation, but increased levels are observed after differentiation. Thus, we evaluated the effects of SFs from hNS/PCs-OE on the viability, proliferation, and differentiation potential of adult murine hippocampal neural precursor cells (AHPCs). SFs from hNS/PCs-OE maintain cells in the precursor and proliferative stages and mainly promote the astrocytic differentiation of AHPCs. These effects involved the activation, as measured by phosphorylation, of several proteins (Erk1/2; Akt/PRAS40/GSK3ß and JAK/STAT) involved in key events of the neurogenic process. Moreover, according to the results from the antibody-based microarray approach, among the soluble factors, hNS/PCs-OE produce interleukin-6 (IL-6) and neurotrophin 4 (NT4). However, residual epidermal growth factor (EGF) was also detected. These proteins partially reproduced the effects of SFs from hNS/PCs-OE on AHPCs, and the mechanism underlying these effects is mediated by Src proteins, which have been implicated in EGF-induced transactivation of TrkB receptor. The results of the present study suggest the potential use of SFs from hNS/PCs-OE in controlling the differentiation potential of AHPCs. Thus, the potential clinical relevance of hNS/PCs-OE is worth pursuing.
Subject(s)
Cell Lineage , Hippocampus/cytology , Neural Stem Cells/cytology , Olfactory Mucosa/cytology , Adult , Animals , Antibodies, Neutralizing/pharmacology , Astrocytes/cytology , Astrocytes/drug effects , Biomarkers/metabolism , Cell Differentiation/drug effects , Cell Lineage/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Culture Media, Conditioned/pharmacology , Cytokines/metabolism , Epidermal Growth Factor/pharmacology , ErbB Receptors/metabolism , Humans , MAP Kinase Signaling System/drug effects , Mice, Inbred C57BL , Neural Stem Cells/drug effects , Neural Stem Cells/metabolism , Phosphorylation/drug effects , Receptor, trkB/metabolism , Solubility , Transcriptional Activation/drug effectsABSTRACT
In this study, we analyzed soluble factors secreted by two Estrogen Receptor Positive (ER-α) human breast cancer cell lines, ZR 75.30 (luminal B) and MCF7 (luminal A), and evaluated their effect on endothelial activation. The composition of tumoral soluble factors (TSFs) was analyzed by ELISA (Bio-Plex). TSFs from ZR 75.30 cells expressed higher levels of TNF, IFN-γ, IL-6, and IL-8 compared to TSFs from MCF-7 cells. TSFs from ZR 75.30 cells induced a pro-adhesive phenotype in human umbilical vein endothelial cells (HUVECs), as characterized by increased monocytic cell adhesion, adhesion molecule expression and NF-κB activation and decreased IκB-α expression. Conversely, TSFs from MCF-7 cells exerted none of these effects on HUVECs. We then added TNF, IFN-γ, IL-6 or IL-8 alone or in combination with TSFs from MCF-7 cells to HUVECs. Only the combinations that included TNF induced endothelial activation. A neutralizing antibody against IL-1ß (this cytokine was not measured in the ELISA) had a modest blocking effect on cellular adhesion or the expression of adhesion molecules induced by TSFs from ZR 75.30 cells in HUVECs. However neutralizing antibodies against TNF, IFN-γ, IL-6 or IL-8 had no effect. Our results suggest that although TNF is an inducer of endothelial cell activation, it is not the only molecule that is responsible for this effect in TSFs from ZR 75.30 cells.
ABSTRACT
RESUMEN En los últimos años, las células troncales mesenquimales (Mesenchymal Stem Cells, MSC) han adquirido mucha importancia debido a su gran plasticidad y su capacidad de liberar factores paracrinos con capacidad de interactuar con diversos tipos celulares, tejidos y órganos. El uso de MSC en medicina regenerativa es importante debido a que, al no expresar las moléculas del complejo mayor de histocompatibilidad (MHC) clase II ni moléculas co-estimuladoras y tener baja expresión del MHC clase I, haría que no sean rechazadas por individuos de la misma especie, es posible utilizarlas no sólo de manera autóloga, sino también, eventualmente, alogénica. Sin embargo, es importante demostrar científicamente muchas de sus propiedades, entre ellas las inmunomoduladoras. Al tener varias fuentes de obtención, se debe estandarizar la que sea la mejor para garantizar la pureza y calidad de las MSC. Finalmente, es importante que cuando se trabaje con estas células se demuestre completamente las características del cultivo celular, la inmunotipificación y su capacidad de diferenciación. Se están ensayando muchas aplicaciones clínicas de las MSC. Dentro de ellas, su capacidad para mejorar la recuperación y potencial curación de úlceras crónicas como las diabéticas, ha atraído la atención por su potencial impacto terapéutico.
ABSTRACT In recent years, mesenchymal stem cells (MSC) have become very important due to their high plasticity and their ability to release paracrine factors able to interact with various cell types, tissues and organs. The use of MSC in regenerative medicine became of vital importance, since they do not express histocompatibility MHC molecules class II nor costimulant molecules, and low expression of MHC class I, will not be rejected by individuals of same species, they could be used in an autologous, and eventually, allogeneic manner. However, it is important to scientifically demonstrate many properties, including immunomodulatory ones. Having several sources of obtaining, it should be standardized the best one to ensure the purity and quality of these cells. Finally, it is important when working with these cells, that characteristics of cell culture, immunophenotyping and differentiation capacity are fully demonstrated. MSC have been applied in several clinical uses. Among them, their ability to improve, and even heal chronic ulcers, as diabetic, has attracted attention for its potential therapeutic impact.
Subject(s)
Humans , Mesenchymal Stem Cells , Mesenchymal Stem Cell TransplantationABSTRACT
Synaptic activity is a critical determinant in the formation and development of excitatory synapses in the central nervous system (CNS). The excitatory current is produced and regulated by several ionotropic receptors, including those that respond to glutamate. These channels are in turn regulated through several secreted factors that function as synaptic organizers. Specifically, Wnt, brain-derived neurotrophic factor (BDNF), fibroblast growth factor (FGF), and transforming growth factor (TGF) particularly regulate the N-methyl-D-aspartate receptor (NMDAR) glutamatergic channel. These factors likely regulate early embryonic development and directly control key proteins in the function of important glutamatergic channels. Here, we review the secreted molecules that participate in synaptic organization and discuss the cell signaling behind of this fine regulation. Additionally, we discuss how these factors are dysregulated in some neuropathologies associated with glutamatergic synaptic transmission in the CNS.
Subject(s)
Receptors, N-Methyl-D-Aspartate/metabolism , Animals , Brain-Derived Neurotrophic Factor/metabolism , Fibroblast Growth Factors/metabolism , Humans , Solubility , Transforming Growth Factors/metabolism , Wnt Proteins/metabolismABSTRACT
ANTECEDENTES: La interacción entre los espermatozoides con algunas especies bacterianas o sus factores solubles influyen en el deterioro de la calidad seminal, alterando la función reproductiva del hombre. OBJETIVO: El objetivo de este trabajo fue determinar el efecto de los factores solubles de Staphylococcus aureus, Staphylococcus capitis y Staphylococcus epidermidis sobre la calidad seminal. MÉTODO: Los factores solubles producto del metabolismo bacteriano de las cepas de S. aureus y S. Capitis sensible a oxacilina y S. aureus y S. Epidermidis resistente a oxacilina se incubaron con las muestras de semen de 20 voluntarios y se cuantificaron los parámetros seminales convencionales y funcionales por microscopía y citometría de flujo, respectivamente. RESULTADOS: Se observó una disminución en la movilidad espermática con los factores solubles de S. aureus, esta disminución fue mayor con la cepa sensible y el efecto negativo sobre la movilidad fue inmediato. Al incubar los espermatozoides con los factores solubles de S. aureus sensible a oxacilina, se afectaron todos los parámetros funcionales excepto la integridad de la cromatina y se observó menor liberación de especies reactivas de oxígeno; con los factores solubles de la cepa de S. aureus resistente a oxacilina se observó una disminución en la lipoperoxidación de membrana y en la expresión de anexina V. CONCLUSIÓN: Este estudio da cuenta del efecto negativo de los factores solubles de la bacteria S. aureus tanto sensible como resistente a oxacilina sobre los parámetros espermáticos convencionales y funcionales, y por ende en su función reproductiva.
BACKGROUND: The interaction between sperm with some bacteria species and their soluble factors are the deterioration of semen quality by altering the reproductive function of man. AIM: The aim of this study was to determine the effect of soluble factors Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus capitis on semen quality. METHODS: The soluble factors product of bacterial metabolism of the strains of S. aureus and S. capitis methicillin sensitive and S. aureus and S. epidermidis resistant to oxacillin, were incubated with semen samples from 20 volunteers. Subsequently, conventional seminal parameters were measured and functional quantified by microscopy and flow cytometry, respectively. RESULTS: A decrease was observed in sperm motility with soluble factors of S. aureus, this decrease was higher with the sensitive strain that with oxacillin resistant strain and the negative effect on motility was immediate. By incubating the sperm with soluble factor from oxacillin-sensitive S. aureus, all functional parameters were affected except the chromatin integrity and reduced release of reactive oxygen species, mean fluorescence intensity in oxacillin resistant S. aureus strain was decrease in membrane lipid peroxidation and annexin V expression. CONCLUSIONS: This study reports the negative effect of soluble factors of bacteria either S. aureus sensitive and resistant to oxacillin, over conventional and functional sperm parameters, and therefore in their reproductive function.
Subject(s)
Humans , Male , Spermatozoa/metabolism , Spermatozoa/microbiology , Staphylococcus aureus/metabolism , Staphylococcus epidermidis/metabolism , Semen Analysis , Staphylococcus capitis/metabolism , Semen/metabolism , Semen/microbiology , Solubility , Sperm Motility/physiology , Bacteria/metabolism , Lipid Peroxidation , Reactive Oxygen Species , Membrane Potential, Mitochondrial , Flow CytometryABSTRACT
Introducción: el actual renacimiento y auge de la medicina regenerativa y el conocimiento de la amplia gama de posibilidades terapéuticas que brinda a la ciencia estomatológica, cobra creciente interés. Objetivo: realizar una revisión bibliográfica sobre la medicina regenerativa en la rama estomatológica en sus aspectos generales. Métodos: se realizó una revisión bibliográfica en el periodo comprendido entre abril y mayo de 2013. Se evaluaron revistas de impacto de Web of Sciencies (36 revistas) y tres revistas cubanas. Se consultaron las bases de datos de sistemas referativos, como MEDLINE, PubMed y Scielo con la utilización de descriptores como regenerative medicine dentistry, stem cells, tissue engineering y su contraparte en español. Se incluyeron artículos en idioma inglés y español y publicaciones de los últimos cinco años, con solo un artículo con mayor tiempo de publicación. Se obtuvieron 120 artículos, circunscribiéndose el estudio a 56 que enfocaron estas temáticas de manera más integral. Se revisó un libro. Resultados: al analizar el comportamiento de los artículos respecto a su representatividad en las diferentes revistas científicas donde fueron publicados, 7,02 por ciento de los consultados correspondió a la Revista Cubana de Hematología, Inmunología y Hemoterapia, lo que resalta la importancia dada al tema en el terreno nacional. Los demás artículos estuvieron distribuidos de manera dispersa entre las otras revistas. Conclusiones: el conocimiento de los cimientos teóricos de la medicina regenerativa empleados para reemplazar, por células sanas, a las células dañadas por diversos procesos en determinados tejidos; justifica la potencialidad de su empleo en la profesión estomatológica bajo el correcto manejo de los conceptos de medicina regenerativa, células madre (con sus mecanismos de acción, tipos, fuentes de obtención), factores solubles, trasplante de genes e ingeniería de tejidos(AU)
Introduction: the current revival and rise of regenerative medicine and the knowledge of the wide range of therapeutic possibilities that science provides dental, increased interest charges. Objective: to review the literature on regenerative medicine in the dental branch in its general aspects. Métodos: A literature review was conducted in the period between April and May 2013. Impact journals were evaluated Web of Sciences (36 journals) and 3 Cuban magazines. Were consulted databases systems such as MEDLINE, PubMed and SciELO with the use of descriptors such as regenerative medicine dentistry, stem cells, tissue engineering and its Spanish counterpart. We included articles in English and Spanish and publications of the last five years, with only 1 item with a longer publication. 120 articles were obtained, confined the study to 56 that focused these issues more comprehensively. Book was reviewed. Results: analysing the behavior of items regarding their representation in various scientific journals where they were published, 7.02 percent of viewed items corresponded to the Cuban Journal of hematology, immunology and blood therapy, underscoring the importance given to the issue in the national arena. Other items were sparsely distributed among the other magazines. Conclusions: knowledge of the theoretical foundations of regenerative medicine to replace employees for healthy cells, damaged cells by various processes in certain tissues; justifies the potential use in the dental profession under the proper management of medical concepts regenerative stem cells (and their mechanisms of action types, obtaining sources), soluble factors, genes transplantation and tissue engineering(AU)
Subject(s)
Humans , Stem Cells , Stomatognathic Diseases/therapy , Tissue Engineering/methods , Regenerative Medicine/methods , Databases, Bibliographic/statistics & numerical dataABSTRACT
Introducción: el actual renacimiento y auge de la medicina regenerativa y el conocimiento de la amplia gama de posibilidades terapéuticas que brinda a la ciencia estomatológica, cobra creciente interés. Objetivo: realizar una revisión bibliográfica sobre la medicina regenerativa en la rama estomatológica en sus aspectos generales. Métodos: se realizó una revisión bibliográfica en el periodo comprendido entre abril y mayo de 2013. Se evaluaron revistas de impacto de Web of Sciencies (36 revistas) y 3 revistas cubanas. Se consultaron las bases de datos de sistemas referativos, como MEDLINE, PubMed y Scielo con la utilización de descriptores como ®regenerative medicine dentistry¼, ®stem cells¼, ®tissue engineering¼ y su contraparte en español. Se incluyeron artículos en idioma inglés y español y publicaciones de los últimos 5 años, con solo 1 artículo con mayor tiempo de publicación. Se obtuvieron 120 artículos. El estudio se circunscribió a 56 que enfocaron estas temáticas de manera más integral. Se revisó un libro. Resultados: al analizar el comportamiento de los artículos respecto a su representatividad en las diferentes revistas científicas donde fueron publicados, 7,02 por ciento de los artículos consultados correspondió a la Revista Cubana de Hematología, Inmunología y Hemoterapia, lo que resalta la importancia dada al tema en el terreno nacional. Los demás artículos estuvieron distribuidos de manera dispersa entre las otras revistas. Conclusiones: el conocimiento de los cimientos teóricos de la medicina regenerativa empleados para reemplazar, por células sanas, a las células dañadas por diversos procesos en determinados tejidos, justifica la potencialidad de su empleo en la profesión estomatológica bajo el correcto manejo de los conceptos de medicina regenerativa, células madre (con sus mecanismos de acción, tipos, fuentes de obtención), factores solubles, trasplante de genes e ingeniería de tejidos(AU)
Introduction: the current revival and development of regenerative medicine and its wide range of therapeutic possibilities in dentistry is a topic of growing interest. Objective: carry out a bibliographic review about the general aspects of regenerative medicine in dentistry. Methods: a bibliographic review was performed which covered the period April-May 2013. An evaluation was conducted of high impact journals from the Web of Sciences (36 journals) and 3 Cuban journals. Databases from reference systems such as MEDLINE, PubMed and Scielo were consulted with the aid of search terms like "regenerative medicine dentistry", "stem cells", "tissue engineering" and their Spanish counterparts. The review included papers in English and Spanish, and publications from the last five years. Only one paper had been published before that period. Of the 120 papers obtained, the reviewers selected the 56 which approached the study topic in a more comprehensive manner. One book was reviewed. Results: an analysis of the representativeness of papers in the scientific journals where they were published showed that 7,02 per cent corresponded to the Cuban Journal of Hematology, Immunology and Hemotherapy, a sign of the importance awarded to the subject in the country. The remaining papers were scattered among the other journals. Conclusions: knowledge about the theoretical foundations of regenerative medicine as applied to the substitution of healthy cells for cells damaged by various processes in certain tissues, justifies its potential use in dentistry, based on the correct application of the concepts of regenerative medicine, stem cells (including its action mechanisms, types, sources), soluble factors, gene transplantation and tissue engineering(AU)
Subject(s)
Regenerative Medicine/methods , Dental Care , Stem Cells , Tissue EngineeringABSTRACT
El actual renacimiento y auge de la medicina regenerativa y el conocimiento de la amplia gama de posibilidades terapéuticas que brinda a la ciencia estomatológica, cobra creciente interés. Objetivo: realizar una revisión bibliográfica sobre la medicina regenerativa en la rama estomatológica en sus aspectos generales. Métodos: se realizó una revisión bibliográfica en el periodo comprendido entre abril y mayo de 2013. Se evaluaron revistas de impacto de Web of Sciencies (36 revistas) y 3 revistas cubanas. Se consultaron las bases de datos de sistemas referativos, como MEDLINE, PubMed y Scielo con la utilización de descriptores como ®regenerative medicine dentistry, ®stem cells, ®tissue engineering y su contraparte en español. Se incluyeron artículos en idioma inglés y español y publicaciones de los últimos 5 años, con solo 1 artículo con mayor tiempo de publicación. Se obtuvieron 120 artículos. El estudio se circunscribió a 56 que enfocaron estas temáticas de manera más integral. Se revisó un libro. Resultados: al analizar el comportamiento de los artículos respecto a su representatividad en las diferentes revistas científicas donde fueron publicados, 7,02 por ciento de los artículos consultados correspondió a la Revista Cubana de Hematología, Inmunología y Hemoterapia, lo que resalta la importancia dada al tema en el terreno nacional. Los demás artículos estuvieron distribuidos de manera dispersa entre las otras revistas. Conclusiones: el conocimiento de los cimientos teóricos de la medicina regenerativa empleados para reemplazar, por células sanas, a las células dañadas por diversos procesos en determinados tejidos, justifica la potencialidad de su empleo en la profesión estomatológica bajo el correcto manejo de los conceptos de medicina regenerativa, células madre (con sus mecanismos de acción, tipos, fuentes de obtención), factores solubles, trasplante de genes e ingeniería de tejidos(AU)
The current revival and development of regenerative medicine and its wide range of therapeutic possibilities in dentistry is a topic of growing interest. Objective: carry out a bibliographic review about the general aspects of regenerative medicine in dentistry. Methods: a bibliographic review was performed which covered the period April-May 2013. An evaluation was conducted of high impact journals from the Web of Sciences (36 journals) and 3 Cuban journals. Databases from reference systems such as MEDLINE, PubMed and Scielo were consulted with the aid of search terms like regenerative medicine dentistry, stem cells, tissue engineering and their Spanish counterparts. The review included papers in English and Spanish, and publications from the last five years. Only one paper had been published before that period. Of the 120 papers obtained, the reviewers selected the 56 which approached the study topic in a more comprehensive manner. One book was reviewed. Results: an analysis of the representativeness of papers in the scientific journals where they were published showed that 7,02 per cent corresponded to the Cuban Journal of Hematology, Immunology and Hemotherapy, a sign of the importance awarded to the subject in the country. The remaining papers were scattered among the other journals. Conclusions: knowledge about the theoretical foundations of regenerative medicine as applied to the substitution of healthy cells for cells damaged by various processes in certain tissues, justifies its potential use in dentistry, based on the correct application of the concepts of regenerative medicine, stem cells (including its action mechanisms, types, sources), soluble factors, gene transplantation and tissue engineering(AU)
Subject(s)
Humans , Dental Care , Stem Cells , Tissue Engineering , Regenerative Medicine/methods , Review Literature as Topic , Databases, Bibliographic/statistics & numerical dataABSTRACT
Os eventos que fazem parte do processo de reparação de lesões da córnea ocorrem simultaneamente e envolvem proliferação, migração, diferenciação e apoptose celular, além da comunicação intercelular. Vários fatores solúveis, além de proteínas da matriz mesenquimal, proteoglicanos, enzimas proteolíticas e alguns tipos celulares são abordados nesta revisão, na qual explicam-se os processos de reparação de lesões superficiais ou penetrantes da córnea. A membrana amniótica, muito utilizada na cirurgia oftálmica, foi estudada por apresentar funções que colaboram com o processo de reparação. Entretanto, tais funções poderão ser perdidas quando tal tecido for submetido à conservação. Assim, torna-se importante conhecer o processo de reparação de lesões que envolvem, ou não, a córnea em toda a sua espessura e escolher a melhor forma de utilização da membrana amniótica quando ela for indicada na terapia para estas lesões.
The events included in the process of repair of corneal damage occur simultaneously and involve proliferation, migration, differentiation, cell apoptosis and intercellular communication. Several soluble factors, mesenchymal matrix proteins, proteoglycans, proteolytic enzymes and some cell types are covered in this review, which explains the processes of repair of corneal wounds, either superficial or penetrating. The amniotic membrane, used in ophthalmic surgery, was studied because of the contribution of its functions to the repair process. However, these functions may be lost when the amniotic membrane is subjected to conservation. Therefore, it is important to understand the repair process of lesions involving or not the entire thickness of the cornea, and choose the best use of the amniotic membrane, when it is indicated for the treatment of these lesions.
ABSTRACT
Infection with Human immunodeficiency virus type-1 (HIV-1) induces severe alterations of the immune system leading to an increased susceptibility to opportunistic infections and malignancies. However, exposure to the virus does not always results in infection. Indeed, there exist individuals who have been repeatedly exposed to HIV-1 but do not exhibit clinical or serological evidence of infection, known as exposed seronegative individuals. Many studies have focused on the different mechanisms involved in natural resistance to HIV-1 infection, and have reported several factors associated with this phenomenon, including the presence of genetic polymorphisms in the viral coreceptors, innate and adaptive immune cells with particular phenotypic and functional features, and molecules such as antibodies and soluble factors that play an important role in defense against infection by HIV-1. The study of these factors could be the key for controlling this viral infection. This review summarizes the main mechanisms involved in resistance to HIV-1 infection.
ABSTRACT
The events included in the process of repair of corneal damage occur simultaneously and involve proliferation, migration, differentiation, cell apoptosis and intercellular communication. Several soluble factors, mesenchymal matrix proteins, proteoglycans, proteolytic enzymes and some cell types are covered in this review, which explains the processes of repair of corneal wounds, either superficial or penetrating. The amniotic membrane, used in ophthalmic surgery, was studied because of the contribution of its functions to the repair process. However, these functions may be lost when the amniotic membrane is subjected to conservation. Therefore, it is important to understand the repair process of lesions involving or not the entire thickness of the cornea, and choose the best use of the amniotic membrane, when it is indicated for the treatment of these lesions.
Os eventos que fazem parte do processo de reparação de lesões da córnea ocorrem simultaneamente e envolvem proliferação, migração, diferenciação e apoptose celular, além da comunicação intercelular. Vários fatores solúveis, além de proteínas da matriz mesenquimal, proteoglicanos, enzimas proteolíticas e alguns tipos celulares são abordados nesta revisão, na qual explicam-se os processos de reparação de lesões superficiais ou penetrantes da córnea. A membrana amniótica, muito utilizada na cirurgia oftálmica, foi estudada por apresentar funções que colaboram com o processo de reparação. Entretanto, tais funções poderão ser perdidas quando tal tecido for submetido à conservação. Assim, torna-se importante conhecer o processo de reparação de lesões que envolvem, ou não, a córnea em toda a sua espessura e escolher a melhor forma de utilização da membrana amniótica quando ela for indicada na terapia para estas lesões.
ABSTRACT
The events included in the process of repair of corneal damage occur simultaneously and involve proliferation, migration, differentiation, cell apoptosis and intercellular communication. Several soluble factors, mesenchymal matrix proteins, proteoglycans, proteolytic enzymes and some cell types are covered in this review, which explains the processes of repair of corneal wounds, either superficial or penetrating. The amniotic membrane, used in ophthalmic surgery, was studied because of the contribution of its functions to the repair process. However, these functions may be lost when the amniotic membrane is subjected to conservation. Therefore, it is important to understand the repair process of lesions involving or not the entire thickness of the cornea, and choose the best use of the amniotic membrane, when it is indicated for the treatment of these lesions.
Os eventos que fazem parte do processo de reparação de lesões da córnea ocorrem simultaneamente e envolvem proliferação, migração, diferenciação e apoptose celular, além da comunicação intercelular. Vários fatores solúveis, além de proteínas da matriz mesenquimal, proteoglicanos, enzimas proteolíticas e alguns tipos celulares são abordados nesta revisão, na qual explicam-se os processos de reparação de lesões superficiais ou penetrantes da córnea. A membrana amniótica, muito utilizada na cirurgia oftálmica, foi estudada por apresentar funções que colaboram com o processo de reparação. Entretanto, tais funções poderão ser perdidas quando tal tecido for submetido à conservação. Assim, torna-se importante conhecer o processo de reparação de lesões que envolvem, ou não, a córnea em toda a sua espessura e escolher a melhor forma de utilização da membrana amniótica quando ela for indicada na terapia para estas lesões.