ABSTRACT
Objective To evaluate the effects of 3 kinds of ELISA reagents on the detection of human paragonimiasis. Meth?ods A total of 45 serum samples from patients with paragonimiasis,218 serum samples from patients with other parasitic dis?eases as well as 80 serum samples from healthy people were detected by GD?ELISA(IgG antigen ELISA detection reagent),ES?ELISA(using excretory/secretory(ES)products of Paragonimus westermani),and sAg?ELISA(using semi?purified antigen (sAg)of P. westermani),respectively. The effects of the 3 reagents were evaluated and compared. Results The sensitivities of GD?ELISA,ES?ELISA,and sAg?ELISA were 95.6%(95%CI:89.6%~100.0%),93.3%(95%CI:86.0%~100.0%)and 86.7%(95%CI:76.8%~96.6%),respectively;the specificities of the above three reagents were 88.6%(95%CI:85.0%~92.2%),88.9%(95%CI:85.3%~92.5%)and 99.0%(95%CI:97.9%~100.0%),respectively,and the Youden indexes of them were 0.84,0.82 and 0.86,respectively. Conclusion sAg?ELISA is more suitable than GD?ELISA and ES?ELISA for clin?ical sample tests in paragonimiasis endemic areas in China.
ABSTRACT
Objective To explore ultrasonographic diagnosis of severe fetal limb deformity in early pregnancy.Methods A total of 5 843 cases of pregnant women were screened and 147 cases of suspected fetal malformation were sellected.The routine ul-trasound examination results were recorded as control group,and continuous order tracking ultrasonic method inspection results were recorded as observation group.The accuracy,sensitivity,specificity,positive predictive value,and negative predictive value was compared.Results The clinical results obtained in multiparous or limb malformation fetus were 40 cases,accounting for 27.21%. In the observation group on limb loss and other detection rate of 100% (14/14)and 65.00% (13/20),significantly higher than those in control group 50.00% (7/14)and 5.00% (1/20).But in the observation group on finger deformity examination showed no advantage.Observation accuracy,sensitivity and negative predictive value was 91.84%,70.00%,89.92%,which were higher than those in the control group(78.91%,22.50%,77.54%).The differences were statistically significant (P <0.05).Conclusion The application of continuous order tracking ultrasonic method to check the first trimester fetal limb deformity with high accuracy,pro-vided sufficient time for prenatal diagnosis of fetal treatment and late.
