A simple chemiluminescent method for the quantification of exosomes based on horseradish peroxidase adsorbed on two-dimensional nanomaterials.

The development of simple methods for the isolation and quantification of exosomes in biological samples is important. By using the typical two-dimensional (2D) nanomaterials, graphene oxide (GO), the present work first studied the interaction of liposomes with the nanocomposites formed by adsorbing HRP on the GO surface and found the presence of liposomes led to the release of HRP from the GO surface to the solution phase triggering the luminol-H2O2 chemiluminescence (CL) reaction to emit light. Benefiting from the similarity of exosomes to liposomes in both composition and morphology aspects, the GO-HRP nanocomposites with a mass ratio of 120:1 and 160:1 were employed for the quantitative detection of exosomes in 100-fold diluted serum samples. The whole detection process took about 15 min and as low as 3.2 × 102 particles µL-1 of exosomes could be sensitively detected. In addition to GO-HRP nanocomposites, the CL responses of other nanocomposites obtained from adsorbing HRP on other 2D nanomaterials such as layered MoS2 for exosomes were also tested. MoS2-HRP exhibited similar behavior and the LODs for the detection of exosomes were 5.8 × 102 particles µL-1. The proposed assays were a biomarker-independent quantitative method that achieved the quantification of exosomes in serum samples directly without an isolation process.

Two-Dimensional Layered Nanomaterials Steering Self-Assembly of Dodecapeptides with Three Building Blocks.

Self-assembly of peptides on layered nanomaterials such as graphite and MoS2 in the formation of long-range ordered two-dimensional nanocrystal patterns leading to its potential applications for biosensing and bioelectronics has attracted significant interest in nanoscience and nanotechnology. However, controlling the self-assembly of peptides on nanomaterials is still challenging due to the unclear role of nanomaterials in steering self-assembly. Here, we used the in-situ AFM technique to capture different changes of peptide coverage as well as lengthening and widening rates depending on peptide concentrations, show the distinct boundary dynamics of two stabilized peptide domains, and resolve the molecular resolution structural differences and specific orientation of peptide on both nanomaterials. Moreover, ex-situ results showed that the nanomaterial layers tuned the opposite changes of nanowire heights and densities and displayed the different water-resistance stabilities on both nanomaterials. This work provides a basis for understanding nanomaterials steering peptide self-assembly and using hybrid bionanomaterials as a scaffold, enabling for potential biosensing and bioelectronics applications.

Potential Application Prospects of Biomolecule-Modified Two-Dimensional Chiral Nanomaterials in Biomedicine.

Chirality, one of the most fundamental properties of natural molecules, plays a significant role in biochemical reactions. Nanomaterials with chiral characteristics have superior properties, such as catalytic properties, optoelectronic properties, and photothermal properties, which have significant potential for specific applications in nanomedicine. Biomolecular modifications such as nucleic acids, peptides, proteins, and polysaccharides are sources of chirality for nanomaterials with great potential for application in addition to intrinsic chirality, artificial macromolecules, and metals. Two-dimensional (2D) nanomaterials, as opposed to other dimensions, due to proper surface area, extensive modification sites, drug loading potential, and simplicity of preparation, are prepared and utilized in diagnostic applications, drug delivery research, and tumor therapy. Current advanced studies on 2D chiral nanomaterials for biomedicine are focused on novel chiral development, structural control, and materials sustainability applications. However, despite the advances in biomedical research, chiral 2D nanomaterials still confront challenges such as the difficulty of synthesis, quality control, batch preparation, chiral stability, and chiral recognition and selectivity. This review aims to provide a comprehensive overview of the origins, synthesis, applications, and challenges of 2D chiral nanomaterials with biomolecules as cargo and chiral modifications and highlight their potential roles in biomedicine.

Nanotoxicity of two-dimensional nanomaterials on human skin and the structural evolution of keratin protein.

Two-dimensional (2D) materials have been increasingly widely used in biomedical and cosmetical products nowadays, yet their safe usage in human body and environment necessitates a comprehensive understanding of their nanotoxicity. In this work, the effect of pristine graphene and graphene oxide (GO) on the adsorption and conformational changes of skin keratin using molecular dynamics simulations. It is found that skin keratin can be absorbed through various noncovalent driving forces, such as van der Waals (vdW) and electrostatics. In the case of GO, the oxygen-containing groups prevent tighter contact between skin keratin and the graphene basal plane through steric effects and electrostatic repulsion. On the other hand, electrostatic attraction and hydrogen bonding enhance their binding affinity to positively charged residues such as lysine and arginine. The secondary structure of skin keratin is better preserved in GO system, suggesting that GO has good biocompatibility. The charged groups on GO surface perform as the hydrogen bond acceptors, which is like to the natural receptors of keratin in this physiological environment. This work contributes to a better knowledge of the nanotoxicity of cutting-edge 2D materials on human health, thereby advancing their potential biological applications.

Two-dimensional nanomaterials induced nano-bio interfacial effects and biomedical applications in cancer treatment.

Two-dimensional nanomaterials (2D NMs), characterized by a large number of atoms or molecules arranged in one dimension (typically thickness) while having tiny dimensions in the other two dimensions, have emerged as a pivotal class of materials with unique properties. Their flat and sheet-like structure imparts distinctive physical, chemical, and electronic attributes, which offers several advantages in biomedical applications, including enhanced surface area for efficient drug loading, surface-exposed atoms allowing precise chemical modifications, and the ability to form hierarchical multilayer structures for synergistic functionality. Exploring their nano-bio interfacial interactions with biological components holds significant importance in comprehensively and systematically guiding safe applications. However, the current lack of in-depth analysis and comprehensive understanding of interfacial effects on cancer treatment motivates our ongoing efforts in this field. This study provides a comprehensive survey of recent advances in utilizing 2D NMs for cancer treatment. It offers insights into the structural characteristics, synthesis methods, and surface modifications of diverse 2D NMs. The investigation further delves into the formation of nano-bio interfaces during their in vivo utilization. Notably, the study discusses a wide array of biomedical applications in cancer treatment. With their potential to revolutionize therapeutic strategies and outcomes, 2D NMs are poised at the forefront of cancer treatment, holding the promise of transformative advancements.

Recent advances in two-dimensional nanomaterials for sustainable wearable electronic devices.

The widespread adoption of smart terminals has significantly boosted the market potential for wearable electronic devices. Two-dimensional (2D) nanomaterials show great promise for flexible, wearable electronics of next-generation electronic materials and have potential in energy, optoelectronics, and electronics. First, this review focuses on the importance of functionalization/defects in 2D nanomaterials, a discussion of different kinds of 2D materials for wearable devices, and the overall structure-property relationship of 2D materials. Then, in this comprehensive review, we delve into the burgeoning realm of emerging applications for 2D nanomaterial-based flexible wearable electronics, spanning diverse domains such as energy, medical health, and displays. A meticulous exploration is presented, elucidating the intricate processes involved in tailoring material properties for specific applications. Each research direction is dissected, offering insightful perspectives and dialectical evaluations that illuminate future trajectories and inspire fruitful investigations in this rapidly evolving field.

Advances in the Application of Black Phosphorus-Based Composite Biomedical Materials in the Field of Tissue Engineering.

Black Phosphorus (BP) is a new semiconductor material with excellent biocompatibility, degradability, and optical and electrophysical properties. A growing number of studies show that BP has high potential applications in the biomedical field. This article aims to systematically review the research progress of BP composite medical materials in the field of tissue engineering, mining BP in bone regeneration, skin repair, nerve repair, inflammation, treatment methods, and the application mechanism. Furthermore, the paper discusses the shortcomings and future recommendations related to the development of BP. These shortcomings include stability, photothermal conversion capacity, preparation process, and other related issues. However, despite these challenges, the utilization of BP-based medical materials holds immense promise in revolutionizing the field of tissue repair.

Recent Advances of Emerging Metal-Containing Two-Dimensional Nanomaterials in Tumor Theranostics.

In recent years, metal-containing two-dimensional (2D) nanomaterials, among various 2D nanomaterials have attracted widespread attention because of their unique physical and chemical properties, especially in the fields of biomedical applications. Firstly, the review provides a brief introduction to two types of metal-containing 2D nanomaterials, based on whether metal species take up the major skeleton of the 2D nanomaterials. After this, the synthetical approaches are summarized, focusing on two strategies similar to other 2D nanomaterials, top-down and bottom-up methods. Then, the performance and evaluation of these 2D nanomaterials when applied to cancer therapy are discussed in detail. The specificity of metal-containing 2D nanomaterials in physics and optics makes them capable of killing cancer cells in a variety of ways, such as photodynamic therapy, photothermal therapy, sonodynamic therapy, chemodynamic therapy and so on. Besides, the integrated platform of diagnosis and treatment and the clinical translatability through metal-containing 2D nanomaterials is also introduced in this review. In the summary and perspective section, advanced rational design, challenges and promising clinical contributions to cancer therapy of these emerging metal-containing 2D nanomaterials are discussed.

Interfacial Modification of Lithium Metal Anode by Boron Nitride Nanosheets.

Metallic lithium (Li) is the most attractive anode for Li batteries because it holds the highest theoretical specific capacity (3860 mA h g-1) and the lowest redox potential (-3.040 V vs SHE). However, the poor interface stability of the Li anode, which is caused by the high reactivity and dendrite formation of metallic Li upon cycling, leads to undesired electrochemical performance and safety issues. While two-dimensional boron nitride (BN) nanosheets have been utilized as an interfacial layer, the mechanism on how they stabilize the Li-electrolyte interface remains elusive. Here, we show how BN nanosheet interlayers suppress Li dendrite formation, enhance Li ion transport kinetics, facilitate Li deposition, and reduce electrolyte decomposition. We show through both simulation and experimental data that the desolvation process of a solvated Li ion within the interlayer nanochannels kinetically favors Li deposition. This process enables long cycling stability, reduced voltage polarization, improved interface stability, and negligible volume expansion. Their application as an interfacial layer in symmetric cells and full cells that display significantly improved electrochemical properties is also demonstrated. The knowledge gained in this study provides both critical insights and practical guidelines for designing a Li metal anode with significantly improved performance.

Synthesis of Guanine/Vermiculite Two-Dimensional Nanocomposites for Wireless Humidity Sensing in Nut Storage Environment.

Two-dimensional (2D) nanostructures have the advantages of high specific surface area, easy surface functionalization, abundant active sites, and good compatibility with device integration and can be assembled into three-dimensional structures, which are key to the development of high-performance gas sensors. In this study, 2D vermiculite (VMT) nanosheets and guanine (G), two renewable resources with unique chemical structures, were organically combined to fully use the specificity of their molecular structures and functional activities. Driven by the regulation of 2D VMT nanosheets, guanine/vermiculite (G/VMT)-based 2D nanocomposites with controllable pore structure, multiple binding sites, and unobstructed mass transfer were designed and synthesized. The G/VMT nanocomposite material was used as a quartz crystal microbalance (QCM) electrode-sensitive film material to build a QCM-based humidity sensor. G/VMT-based QCM humidity sensor had good logarithmic linear relation (0.9971), high sensitivity (24.49 Hz/% relative humidity), low hysteresis (1.75% RH), fast response/recovery time (39/6 s), and good stability. Furthermore, with a QCM sensor and a specially designed wireless circuit, a wireless humidity detection system transmitting via Wi-Fi allows real-time monitoring of nut storage. This study presents an environmentally friendly, high-performance, miniature 2D nanocomposite sensor strategy for real-time monitoring.

Review of two-dimensional nanomaterials in tribology: Recent developments, challenges and prospects.

From our ordinary lives to various mechanical systems, friction and wear are often unavoidable phenomena that are heavily responsible for excessive expenditures of nonrenewable energy, the damages and failures of system movement components, as well as immense economic losses. Thus, achieving low friction and high anti-wear performance is critical for minimization of these adverse factors. Two-dimensional (2D) nanomaterials, including transition metal dichalcogenides, single elements, transition metal carbides, nitrides and carbonitrides, hexagonal boron nitride, and metal-organic frameworks have attracted remarkable interests in friction and wear reduction of various applications, owing to their atomic-thin planar morphologies and tribological potential. In this paper, we systematically review the current tribological progress on 2D nanomaterials when used as lubricant additives, reinforcement phases in the coatings and bulk materials, or a major component of superlubricity system. Additionally, the conclusions and prospects on 2D nanomaterials with the existing drawbacks, challenges and future direction in such tribological fields are briefly provided. Finally, we sincerely hope such a review will offer valuable lights for 2D nanomaterial-related researches dedicated on tribology in the future.

Recent Advances in the Preparation and Application of Two-Dimensional Nanomaterials.

Two-dimensional nanomaterials (2D NMs), consisting of atoms or a near-atomic thickness with infinite transverse dimensions, possess unique structures, excellent physical properties, and tunable surface chemistry. They exhibit significant potential for development in the fields of sensing, renewable energy, and catalysis. This paper presents a comprehensive overview of the latest research findings on the preparation and application of 2D NMs. First, the article introduces the common synthesis methods of 2D NMs from both "top-down" and "bottom-up" perspectives, including mechanical exfoliation, ultrasonic-assisted liquid-phase exfoliation, ion intercalation, chemical vapor deposition, and hydrothermal techniques. In terms of the applications of 2D NMs, this study focuses on their potential in gas sensing, lithium-ion batteries, photodetection, electromagnetic wave absorption, photocatalysis, and electrocatalysis. Additionally, based on existing research, the article looks forward to the future development trends and possible challenges of 2D NMs. The significance of this work lies in its systematic summary of the recent advancements in the preparation methods and applications of 2D NMs.

Two-Dimensional Covalent Organic Frameworks as Tailor-Made Scaffolds for Water Harvesting.

Developing materials to harvest water from the air is of great importance to alleviate the water shortage for people living in arid regions, where the annual average relative humidity (RH) is lower than 0.4. In this work, we report a general nitrogen atom incorporation strategy to prepare high-performance covalent organic frameworks (COFs) for water harvesting from the air in arid areas. A series of COFs, namely COF-W1, COF-W2, and COF-W3 were developed for this purpose. Different contents of nitrogen were embedded into COFs by incorporating pyridine units into the building blocks. With the increasing content of nitrogen from COF-W1 to COF-W3, the inflection points of their water isotherms shift distinctly from RH values from 0.65 to 0.25. Significantly, COF-W3 exhibits the lowest inflection point at a low RH value of 0.25 and reaches a high uptake capacity of 0.28 g g-1 at 25 °C with a low hysteresis loop. Moreover, the gram-scale COF-W3 retains its high performance, which renders it more attractive in water harvesting. This work demonstrates the feasibility of this nitrogen incorporation strategy to acquire high-performance COFs as water harvesters in the future.

Two-dimensional nanomaterials as enhanced surface plasmon resonance sensing platforms: Design perspectives and illustrative applications.

Both increasing demand for ultrasensitive detection in the scientific community and significant new breakthroughs in materials science field have inspired and promoted the development of new-generation multifunctional plasmonic sensing platforms by adopting promising plasmonic nanomaterials. Recently, high-quality surface plasmon resonance (SPR) sensors, assisted by two dimensional (2D) nanomaterials including 2D van der Waals (vdWs) materials (such as graphene/graphene oxide, transition metal dichalcogenides (TMDs), phosphorene, antimonene, tellurene, MXenes, and metal oxides), 2D metal-organic frameworks (MOFs), 2D hyperbolic metamaterials (HMMs), and 2D optical metasurfaces, have emerged as a class of novel plasmonic sensing platforms that show unprecedented detection sensitivity and impressive performance. This review of recent progress in 2D nanomaterials-enhanced SPR platforms will highlight their compelling plasmonic enhancement features, working mechanisms, and design methodologies, as well as discuss illustrative practical applications. Hence, it is of great importance to describe the latest research progress in 2D nanomaterials-enhanced SPR sensing cases. In this review, we present some concepts of SPR enhanced by 2D nanomaterials, including the basic principles of SPR, signal modulation approaches, and working enhancement mechanisms for various 2D materials-enhanced SPR systems. In addition, we also demonstrate a detailed categorization of 2D nanomaterials-enhanced SPR sensing platforms and comment on their ability to realize ultrasensitive SPR detection. Finally, we conclude with future perspectives for exploring a new generation of 2D nanomaterials-based sensors.

Revealing Disparities in Porous Networks Between Yttria Aerogel Assemblies with Nanosheets and Nanoparticles and Their Ultrathermal Insulation and Optical Properties.

Recent advancements have introduced anisotropic structures, particularly 2D nanosheets, into aerogels, resulting in unique morphologies and exceptional properties that differ from those assembled by isotropic nanoparticles. However, exploration of the distinct porous networks and the resulting properties is limited. We focus on rare earth yttria (Y2O3) aerogels as a case in point and demonstrate the synthesis of aerogels with nanosheet and nanoparticle assemblies using elaborative sol-gel chemistry. With the aid of X-ray computed tomography, three-dimensional visualizations of the aerogels provide relative compressive views of the porous network, revealing that the Y2O3 aerogel assembled by nanosheets possesses a hierarchical pore structure characterized by uneven pore distribution, particularly the presence of macropores throughout; in contrast, these consist of nanoparticles exhibiting a relative uniform pore distribution. High-temperature examinations indicate that the nanosheet aerogels are much more stable with a specific surface area of 64 m2·g-1 after being exposed at 1300 °C; meanwhile, the aerogels present durable and efficient thermal insulation performances. The exceptional thermal properties are attributed to the synergistic effects of the nanosheets' crystalline nature and the hierarchical porous network. The nanosheet Y2O3 aerogel also exhibited superior luminescent emission characteristics, further enhancing its potential for various applications. Our findings provide further insights into optimization of the microstructures in nanoporous aerogels, particularly through the utilization of anisotropic nanosheets.

In situ Engineering of Tumor-Associated Macrophages via a Nanodrug-Delivering-Drug (ß-Elemene@Stanene) Strategy for Enhanced Cancer Chemo-Immunotherapy.

Tumor-associated macrophages (TAMs) play a critical role in the immunosuppressive solid tumor microenvironment (TME), yet in situ engineering of TAMs for enhanced tumor immunotherapy remains a significant challenge in translational immuno-oncology. Here, we report an innovative nanodrug-delivering-drug (STNSP@ELE) strategy that leverages two-dimensional (2D) stanene-based nanosheets (STNSP) and ß-Elemene (ELE), a small-molecule anticancer drug, to overcome TAM-mediated immunosuppression and improve chemo-immunotherapy. Our results demonstrate that both STNSP and ELE are capable of polarizing the tumor-supportive M2-like TAMs into a tumor-suppressive M1-like phenotype, which acts with the ELE chemotherapeutic to boost antitumor responses. In vivo mouse studies demonstrate that STNSP@ELE treatment can reprogram the immunosuppressive TME by significantly increasing the intratumoral ratio of M1/M2-like TAMs, enhancing the population of CD4+ and CD8+ T lymphocytes and mature dendritic cells, and elevating the expression of immunostimulatory cytokines in B16F10 melanomas, thereby promoting a robust antitumor response. Our study not only demonstrates that the STNSP@ELE chemo-immunotherapeutic nanoplatform has immune-modulatory capabilities that can overcome TAM-mediated immunosuppression in solid tumors, but also highlights the promise of this nanodrug-delivering-drug strategy in developing other nano-immunotherapeutics and treating various types of immunosuppressive tumors.

Sulfonated Polyaniline-Grafted Two-Dimensional Ti3C2-MXene (SPANI-MXene) Nanoplatform for Designing an Advanced Smart Self-Healable Coating System.

MXene nanosheets (MXenes), a brand-new classification of two-dimensional (2D) nanomaterials, are assumed to be highly functional components in anticorrosion polymeric systems. In general, MXenes possess many advantageous features that can be utilized to improve the polymeric matrices' anticorrosion performance. In this work, zinc ions (Zn) were deposited on the sulfonated polyaniline (SPANI) that was polymerized on Ti3C2-MXene surfaces (MXP-Zn) in order to achieve a high-performance anticorrosion nanofiller for epoxy coating (EP-MXP-Zn). Field-emission scanning electron microscopy-transmission electron microscopy images, Fourier transform infrared, Raman, X-ray diffraction, UV-vis, derivative thermogravimetry, and thermogravimetric analysis have evidenced the successful characterization of the MXP-Zn nanocomposite. Likewise, the excellent barrier properties of SPANI, in conjunction with the cathodic protection of Zn, resulted in a novel nanocomposite that could mitigate the negative consequences of destructive ions' attack on the metal surface in an aggressive media. Quantitative and qualitative anticorrosion measurements verified the outstanding anticorrosion performance of EP-MXP-Zn over time in severe conditions. According to the electrochemical impedance spectroscopy assessments, the |Z0.01 Hz| value for EP-MXP-Zn was 1010.04 Ω cm2, which was over 105 times greater than that of neat EP (104.66 Ω cm2) over a 6-week period of immersion in a 3.5 wt % NaCl solution.

Obtaining of ZnO/Fe2O3 Thin Nanostructured Films by AACVD for Detection of ppb-Concentrations of NO2 as a Biomarker of Lung Infections.

ZnO/Fe2O3 nanocomposites with different concentration and thickness of the Fe2O3 layer were obtained by two-stage aerosol vapor deposition (AACVD). It was shown that the ZnO particles have a wurtzite structure with an average size of 51-66 nm, and the iron oxide particles on the ZnO surface have a hematite structure and an average size of 23-28 nm. According to EDX data, the iron content in the films was found to be 1.3-5.8 at.%. The optical properties of the obtained films were studied, and the optical band gap was found to be 3.16-3.26 eV. Gas-sensitive properties at 150-300 °C were studied using a wide group of analyte gases: CO, NH3, H2, CH4, C6H6, ethanol, acetone, and NO2. A high response to 100 ppm acetone and ethanol at 225-300 °C and a high and selective response to 300-2000 ppb NO2 at 175 °C were established. The effect of humidity on the magnitude and shape of the signal obtained upon NO2 detection was studied.

Enhanced Electrochemical Performance of Metallic CoS-Based Supercapacitor by Cathodic Exfoliation.

Two-dimensional nanomaterials hold great promise as electrode materials for the construction of excellent electrochemical energy storage and transformation apparatuses. In the study, metallic layered cobalt sulfide was, firstly, applied to the area of energy storage as a supercapacitor electrode. By a facile and scalable method for cathodic electrochemical exfoliation, metallic layered cobalt sulfide bulk can be exfoliated into high-quality and few-layered nanosheets with size distributions in the micrometer scale range and thickness in the order of several nanometers. With a two-dimensional thin sheet structure of metallic cobalt sulfide nanosheets, not only was a larger active surface area created, but also, the insertion/extraction of ions in the procedure of charge and discharge were enhanced. The exfoliated cobalt sulfide was applied as a supercapacitor electrode with obvious improvement compared with the original sample, and the specific capacitance increased from 307 F∙g-1 to 450 F∙g-1 at the current density of 1 A∙g-1. The capacitance retention rate of exfoliated cobalt sulfide enlarged to 84.7% from the original 81.9% of unexfoliated samples while the current density multiplied by 5 times. Moreover, a button-type asymmetric supercapacitor assembled using exfoliated cobalt sulfide as the positive electrode exhibits a maximum specific energy of 9.4 Wh∙kg-1 at the specific power of 1520 W∙kg-1.

Environmentally sustainable implementations of two-dimensional nanomaterials.

Rapid advancement in nanotechnology has led to the development of a myriad of useful nanomaterials that have novel characteristics resulting from their small size and engineered properties. In particular, two-dimensional (2D) materials have become a major focus in material science and chemistry research worldwide with substantial efforts centered on their synthesis, property characterization, and technological, and environmental applications. Environmental applications of these nanomaterials include but are not limited to adsorbents for wastewater and drinking water treatment, membranes for desalination, and coating materials for filtration. However, it is also important to address the environmental interactions and implications of these nanomaterials in order to develop strategies that minimize their environmental and public health risks. Towards this end, this review covers the most recent literature on the environmental implementations of emerging 2D nanomaterials, thereby providing insights into the future of this fast-evolving field including strategies for ensuring sustainable development of 2D nanomaterials.