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Objective To assess stroke risk in the patients with new-onset atrial fibrillation (AF) after elective percutaneous coronary intervention (PCI) by von Willebrand factor antigen (vWF∶Ag).Methods A total of 127 patients with new-onset atrial fibrillation after PCI (new-onset AF after PCI group),50 new inpatients with acute coronary syndrome without undergoing PCI (ACS control),50 patients without AF after PCI (PCI control) and 50 healthy individuals (healthy control) were selected in Tianjin Medical University General Hospital from 2016 February to 2017 February for retrospective cohort study.To assay the plasma vWF∶Ag for all objects,receiver operating characteristic curve (ROC) was used to analyze the predicting performance of vWF∶Ag with stroke risk in the patients with new-onset AF.x2 test was used to analyze the relevance between vWF∶Ag and clinical pathological factors in the patients with new-onset AF.Kaplan-Meier curve was used to implement survival analysis.Results The results of plasma vWF∶Ag were as follows:104.5％ (88.0％,133.7％) for the control group,161.7％ (120.7％,270.5％) for ACS control group,208％ (125.2％,360.7％) for PCI control group and 284.9％ (172.4％,494.2％) for new-onset AF after PCI group,respectively.The level of plasma vWF∶Ag of PCI control was higher than that of ACS control (U =526.0,P ＜0.01) and the level in new-onset AF after PCI group was higher than that of PCI control (U =824.0,P ＜ 0.01).The area under the curve was 0.882 (95％ CI:0.811-0.854) for plasma vWF∶Ag level predicting stroke risk with new-onset AF.When the cut-off value of vWF∶Ag was defined as 312.0％,the sensitivity was 94.4％ and specificity was 60.6％.Compared with the patients whose vWF∶Ag was less than 312.0％,the cumulative probability increased significantly (Log-rankx2 =44.308,P ＜ 0.01) during 90 days of follow-up period in the patients with new-onset AF after PCI whose vWF∶Ag was over 312.0％.There were relevances between the vWF∶Ag level and chronic heart failure/left ventricular dysfunction,hypertension,age,stroke/transient ischemic attack (TIA)/history of thromboembolism and vascular disease (P ＜ 0.01).The level of plasma vWF∶Ag and risk of stroke event in the patients with dual antithrombotic therapy were higher than those of the patients with triple antithrombotic therapy (U =1 075.5,P ＜ 0.01;x2 =10.45,P ＜ 0.01).Conclusion The level of plasma vWF ∶ Ag could reflect the damage condition of vascular endothelial cells,stroke risk and the efficacy of anticoagulant therapy in the patients with new-onset atrial fibrillation after PCI.
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Objective To understand the correlation between plasma von Wilebrand factor (vWF) changes after stenting and the degree of preoperative intracranial major artery stenosis in patients with acute atherosclerotie cerebral infarction. Methods This study involved 38 consecutive patients with acute cerebral infarction due to intracranial major artery atherosclerosis, who were admitted between February and October 2008 and underwent stent placement in the stenotic arteries. Thirty healthy volunteers were also recruited to serve as the control group. The patients were divided into severe stenosis group (with stenosis of the intracranial major artery≥70%) and non-severe stenosis groups. Venous blood samples were obtained from the subjects on the morning of the first and 7th days after admission to measure the plasma levels of vWF using sandwich enzyme-linked immunosorbent assay. Results The plasma levels of vWF were significantly higher in patients with acute cerebral infarction than in the control group(P=0.000). Compared with those with non-severe stenosis, the patients with severe stenosis exhibited significantly higher plasma levels of vWF (P=0.015) and greater vWF variation after stent placement (P=0.000). Conclusions In patients with acute atherosclerotic cerebral infarction due to severe intracranial major artery stenosis, the plasma levels of vWF and its postoperative variation are positively correlated to the degree of senosis of the culprit arteries, and severer stenosis is associated with greater postoperative damage of the vascular endothelium.
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AIM: To investigate the properties of human retinal microvascular endothelial cells (RMECs) at two different age groups. METHODS: Human RMECs with high affinity were isolated from donors at two age groups: 30 d (group A) after birth and 40 - 60 years of age (group B). The RMECs were characterized for expression and localization of endothelial cell markers by immunofluorescence staining of CD31, yon Willebrand factor(vWF) and uptake of acetylated low - density lipoprotein. The ability of tube formation was assessed on Matrigel, and vWF distribution in- cells was ob-served by confocal immunofluorescence microscope and Western blotting analysis, respectively. RESULTS: High purity RMECs can be obtained readily from each group with modified methods. At 6 hours, cells from both groups formed tube structures successfully, but there was a significantly higher incidence of branching in RMECs of infant group (group A) by 27.2%±2.2% compared with adult group (group B) at 12 h (P<0.05). Group A maintained intact structure even at 30h, but group B partially lost the basic tube structure. In addition, vWF was translocated from cytoplasm to nucleus with aging. CONCLUSION: Human RMECs at different ages have specific properties. Cell properties related with age of the donors should be considered in in vitro studies.
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2 in some patients with the loss of high and medium sized vWF multimers in plasma.Eight patients with vWD were identified, wherein two were characterized as type 1,4 as type 2A and 2 as type 3 respectively.Conclusion The panel of tests is suitable for diagnosis and classification of vWD.