Detecting changes in industrial pollution by analyzing heavy metal concentrations in tree-ring wood from Romanian conifer forests.

The impact of air pollution on forests, especially in urban areas, has been increasingly discussed recently. Many pollutants, including heavy metals, are released into the atmosphere from various sources, such as mining, non-ferrous metal processing plants, and fossil fuel combustion. These pollutants can adversely affect not only tree growth but also other species, including humans. This study compared the concentrations of several elements in tree-ring wood from two conifer species (Silver fir, Abies alba; Norway spruce, Picea abies) growing in polluted and unpolluted areas. Two regions in northern Romania (Bicaz and Tarnița) that were subjected to historical pollution changes were selected. Two chemical analyses were used: inductively coupled plasma mass spectrometry (ICP-MS) and X-ray fluorescence spectrometry (XRF). The silver fir trees from the intensively polluted area in the Tarnița region were negatively impacted by industrial pollution: the Mn concentrations were, on average, three times higher in polluted areas than in unpolluted areas (ca. 30 vs. 10 mg kg-1). This finding was consistent for both ICP-MS and XRF analyses. However, in Norway spruce, this difference was found only in the XRF data, which detected Mn concentrations seven times higher in trees from polluted areas than those from unpolluted areas (ca. 700 vs. 100 mg kg-1). In the Tarnița region, Norway spruce accumulated more heavy metals than silver fir, but the most pronounced differences between polluted and unpolluted areas were found in silver fir. The two analytical methods are commonly used to determine metal concentrations in wood, and they complement each other, with ICP-MS having a low detection limit for some elements and XRF having higher detection limits and better accuracy. Each method has its advantages and disadvantages, and the optimal method depends on many factors, such as the type of heavy metal analyzed, its concentration in wood, sample type, cost, analysis time, and sample preparation.

Preparation of Activated Biocarbons from Cones and their Potential Application for Adsorption of Antibiotics (Tetracycline).

The pine cones (PC), spruce cones (SC) and fir cones (FC) were used for biocarbons preparation. Chemical activation with sodium hydroxide was applied to prepare activated biocarbons. All the materials under investigation were characterized by the N2 adsorption, scanning electron microscopy (SEM), elemental analysis (CHNS), infrared spectroscopy (ATR FT-IR), and the Boehm's titration method. Moreover, pHpzc (the point of zero charge) was determined. It was shown that cones are a good, cheap precursor from which biocarbons with a developed porous structure, characterized by good adsorption properties, can be obtained. All the obtained adsorbents are characterized mainly by a microporous structure. Moreover, they contain both acidic and basic surface functional groups (acidic ones prevail over basic ones). The tested activated biocarbons have large specific surface area values ranging from 578 to 1182 m2 g-1. The efficacy of selected materials in the adsorption of an essential contaminant of increasing concern, tetracycline (TC), was investigated. The experimental data were described using the Langmuir and Freundlich adsorption isotherm models. The maximum adsorption capacity of the tested biocarbons ranges from 200 to 392 mg g-1. Thermodynamic studies proved that adsorption is a spontaneous and endothermic process. In summary, economical and environmentally friendly adsorbents were obtained.

Structurally Diverse Triterpene-26-oic Acids as Potential Dual ACL and ACC1 Inhibitors from the Vulnerable Conifer Keteleeria fortunei.

A preliminary phytochemical investigation on the 90% MeOH extract from the twigs and needles of the vulnerable conifer Keteleeria fortunei led to the isolation and characterization of 17 structurally diverse triterpen-26-oic acids, including nine previously undescribed ones (fortunefuroic acids A-I, 1-9) featuring a rare furoic acid moiety in the lateral chain. Among them, 1-5 are uncommon 9ßH-lanostane-type triterpenoic acids. Friedo-rearranged triterpenoids 6 and 7 feature a unique 17,14-friedo-lanostane skeleton, whereas 9 possesses a rare 17,13-friedo-cycloartane-type framework. Their structures and absolute configurations were elucidated by extensive spectroscopic (e.g., detailed 2D NMR) and computational (NMR/ECD) calculations and the modified Mosher's method. In addition, the absolute structure of compound 1 was ascertained by single-crystal X-ray diffraction analyses. Fortunefuroic acids B (2), G (7), and I (9), along with isomangiferolic acid (12) and 3α,27-dihydroxycycloart-24E-en-26-oic acid (14), exhibited dual inhibitory effects against the adenosine triphosphate (ATP)-citrate lyase (ACL, IC50s: 5.7-11.4 µM) and acetyl-CoA carboxylase 1 (ACC1, IC50s: 7.5-10.5 µM), both of which are key enzymes for glycolipid metabolism. The interactions of the bioactive triterpenoids with both enzymes were examined by molecular docking studies. The above findings reveal the important role of protecting plant species diversity in support of chemical diversity and potential sources of new therapeutics for ACL-/ACC1-associated diseases.

Triterpenoids from the leaves of Abies koreana and their biological activities.

Seven undescribed triterpenoids, abikoranes A-G, along with three known triterpenoids were isolated from the leaves of Abies koreana E. H. Wilson. The structures of compounds were elucidated by 1D and 2D NMR, HRMS, ECD, specific rotation, and DP4+ analysis. Abikorane A represents the second example of nor-3,4-seco-17,14-friedo-lanostane triterpenoid. Among the isolates, some compounds showed strong cytotoxic activities against some of four tested cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-116) with the values of IC50 0.89-9.62 µM, inhibited lipopolysaccharide-stimulated nitric oxide production with IC50 values of 11.57-15.16 µM, and exhibited significant nerve growth factor release effect (192.54 ± 12.33%) from C6 glioma cells.

Chemical constituents from Abies chensiensis and their antifungal activity.

One new (1) and six known cycloartane triterpenoids (2-7), along with seven reported abietane diterpenoids (8-14), were isolated from the bark of the branches of Abies chensiensis, of which compounds 2-14 were also received for the first time from the genus of Abies. Structural elucidation of all the compounds was carried out by extensive spectroscopic analyses. Additionally, the antifungal activity of isolated compounds 2-13 was evaluated by inhibiting the growth of fungal mycelium. Among them, compounds 8 and 10 demonstrated obvious inhibitory activity against plant pathogens Fusarium avenaceum and Bipolaris sorokiniana.

Three New Lanostanoids with Anti-HCV Effects from Abies nukiangensis.

Three new lanostane derivatives (1-3) and twelve known triterpenoids (4-15) were isolated from the twigs and leaves of Abies nukiangensis. The structures of the new compounds were elucidated mainly by detailed analysis of their NMR and HR-ESI-MS spectroscopic data. Evaluation of the anti-HCV effects of all isolates showed that 3 exhibited moderate effect with the EC50 value of 11.09 µM.

Novel Approaches for Inhibiting the Indoor Allergen Der f 2 Excreted from House Dust Mites by Todomatsu Oil Produced from Woodland Residues.

House dust mite (HDM) is a globally ubiquitous domestic cause of allergic diseases. There is a pressing demand to discover efficient, harmless, and eco-friendly natural extracts to inhibit HDM allergens that are more likely to trigger allergies and challenging to be prevented entirely. This study, therefore, is aimed at assessing the inhibition of the allergenicity of major HDM allergen Der f 2 by todomatsu oil extracted from residues of Abies Sachalinensis. The inhibition was investigated experimentally (using enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)) and in silico using molecular docking. The results showed that todomatsu oil inhibits the allergenicity of Der f 2 by reducing its amount instead of the IgG binding capacity of a single protein. Moreover, the compounds in todomatsu oil bind to Der f 2 via alkyl hydrophobic interactions. Notably, most compounds interact with the hydrophobic amino acids of Der f 2, and seven substances interact with CYS27. Contrarily, the principal compounds fail to attach to the amino acids forming the IgG epitope in Der f 2. Interestingly, chemical components with the lowest relative percentages in todomatsu oil show high-affinity values on Der f 2, especially ß-maaliene (-8.0 kcal/mol). In conclusion, todomatsu oil has been proven in vitro as a potential effective public health strategy to inhibit the allergenicity of Der f 2.

Abieshanesides A and B, two unique ent-18,19-dinoricetexane diterpenoid glycosides from Abies beshanzuensis M.H. Wu.

Two unprecedented ent-18,19-dinoricetexane diterpenoid glycosides, named abieshanesides A (1) and B (2), together with seven known compounds, have been isolated from the dead trunks and branches of Abies beshanzuensis M.H. Wu. To our knowledge, abieshanesides A and B represent the first ent-18,19-dinoricetexane diterpenoid glycosides found in natural sources. Their structures and absolute configurations were elucidated by using a combination of spectroscopic techniques and comparison of experimental and calculated electronic circular dichroism (ECD) data. The MTT experiments showed that (E)-resveratrol (7) could inhibit viability of MH7A cells with the IC50 value of 12.5 µM. Compound 7 was able to block MH7A cell proliferation and was associated with G0/G1-phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 7 significantly induced the proliferation of MH7A cells in a dose-dependent manner.

Comparison of the Content of Extractives in the Bark of the Trunk and the Bark of the Branches of Silver Fir (Abies alba Mill.).

The main objective of our study was to investigate the possible differences in the chemical composition of extractives from the bark of silver fir (Abies alba) with respect to the location of the bark sample on the tree, viz. differences in extract composition between stem bark and branch bark samples. Extractives in the bark samples from branches, depending on the distance of the sample from the trunk, were also analysed, and the stem bark samples were analysed with respect to their inner and outer parts. The results of the chemical analysis of extractives were supported by information about their antifungal and antioxidant effects. After felling and sampling silver fir trees, the collected bark samples were ground and freeze-dried. Extraction of bark samples was followed by a system of accelerated extraction using only water as a solvent. The extracts were analysed chemically using gravimetry, spectrophotometry and chromatography. Free-radical-scavenging activity was measured using the DPPH method, and the antifungal effect towards three moulds and three wood-decaying fungi was investigated with antifungal assay using the agar well diffusion method. It was found that the moisture content in bark samples decreased intensively just after the bark samples were peeled off the stem. Detailed chromatographic analysis showed that the bark extracts contained 14 compounds, among which phenolic acids, flavonoids and lignans were found to be the characteristic ones. The content of hydrophilic extractives in the branch bark samples decreased with increasing distance of the sample location from the tree stem. The largest amounts of phenolic extractives were measured in stem bark, followed by branch bark sampled at the point at which the branch entered the tree. Analysis of the separated parts of the bark showed that the outer layers of stem bark contained larger amounts of phenolic extractives, as well catechin and epicatechin, compared to the inner layers. Concentrated extracts of branch bark showed the largest free-radical-scavenging activity among the investigated samples, while strong antifungal effects of the bark extract were not found.

A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

RATIONALE: The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. METHODS: A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. RESULTS: Peaks corresponding to [M - H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M - H + Ba]+ ions of polyprenols were discussed. CONCLUSIONS: The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.

Effects of Siberian fir terpenes extract Abisil on antioxidant activity, autophagy, transcriptome and proteome of human fibroblasts.

BACKGROUND: Abisil is an extract of Siberian fir terpenes with antimicrobial and wound healing activities. Previous studies revealed that Abisil has geroprotective, anti-tumorigenic, and anti-angiogenic effects. Abisil decreased the expression of cyclin D1, E1, A2, and increased the phosphorylation rate of AMPK. OBJECTIVE: In the present study, we analyzed the effect of Abisil on autophagy, the mitochondrial potential of embryonic human lung fibroblasts. We evaluated its antioxidant activity and analyzed the transcriptomic and proteomic effects of Abisil treatment. RESULTS: Abisil treatment resulted in activation of autophagy, reversal of rotenone-induced elevation of reactive oxygen species (ROS) levels and several-fold decrease of mitochondrial potential. Lower doses of Abisil (25 µg/ml) showed a better oxidative effect than high doses (50 or 125 µg/ml). Estimation of metabolic changes after treatment with 50 µg/ml has not shown any changes in oxygen consumption rate, but extracellular acidification rate decreased significantly. Abisil treatment (5 and 50 µg/ml) of MRC5-SV40 cells induced a strong transcriptomic shift spanning several thousand genes (predominantly, expression decrease). Among down-regulated genes, we noticed an over-representation of genes involved in cell cycle progression, oxidative phosphorylation, and fatty acid biosynthesis. Additionally, we observed predominant downregulation of genes encoding for kinases. Proteome profiling also revealed that the content of hundreds of proteins is altered after Abisil treatment (mainly, decreased). These proteins were involved in cell cycle regulation, intracellular transport, RNA processing, translation, mitochondrial organization. CONCLUSIONS: Abisil demonstrated antioxidant and autophagy stimulating activity. Treatment with Abisil results in the predominant downregulation of genes involved in the cell cycle and oxidative phosphorylation.

All-biobased transparent-wood: A new approach and its environmental-friendly packaging application.

Transparent-wood (TW) is an emerging research topic that can be applied to biobased products. However, it is necessary to fill pores in TW with natural substances to prepare all-biobased TW. This paper reports an all-biobased TW by infiltrating cellulose nanofiber (CNF) and chitosan (CTS) suspensions into the bleached wood. CNF was isolated by combining the chemical and physical methods, and CTS was dissolved in acetic acid, and they were infiltrated into the pores of the bleached Fir veneer wood using a vacuum jar. The CNF and chitosan effects on the mechanical properties of the TW were studied, and the morphologies, crystallinity index, water contact angle, antioxidant, thermal degradation, and UV-shielding properties were investigated. The prepared TW showed 80 % total transmittance and 30-60 % haze, suitable for solar cell application. The all-biobased TW showed good thermal stability up to 315 °C and excellent UV shielding property for UV-B and UV-C. The antioxidant property of the CTS-TW significantly increased as compared to the original wood. The CNF-TW showed considerable tensile strength and yield strength of more than 200 % improved from the original wood. The potential for environment-friendly packaging applications was demonstrated by making a bag, medicine packaging, and straw for a drink.

Terpenoids from Abies holophylla Attenuate LPS-Induced Neuroinflammation in Microglial Cells by Suppressing the JNK-Related Signaling Pathway.

We have previously reported that phytochemicals from Abies holophylla exhibit anti-inflammatory and neuroprotective effects by decreasing nitrite production and increasing nerve growth factor production. However, the exact mechanism underscoring these effects has not been revealed. In the present study, we aimed to explore the underlying anti-inflammatory mechanisms of A. holophylla and its phytochemicals. We studied various solvent fractions of A. holophylla and found the chloroform and hexane sub-fractions showed the most significant anti-neuroinflammatory effects in lipopolysaccharide (LPS)-activated murine microglia. Concomitantly, the terpenoids isolated from chloroform and hexane fractions showed similar anti-neuroinflammatory effects with significant inhibition of NO and reactive oxygen species production, and decreased protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase. Interestingly, these terpenoids inhibited the phosphorylation of c-Jun N-terminal kinase (JNK), which further inhibited the production of pro-inflammatory mediators, including prostaglandin E2, tumor necrosis factor, and interleukins (IL-6 and IL-1ß), with a potency greater than that of the well-known iNOS inhibitor NG-mono-methyl-L-arginine (L-NMMA). These results suggest that the chloroform- and hexane-soluble fraction mediated the mitogen-activated protein kinase (MAPK) inhibition, in particular the JNK pathway, thereby lowering the inflammatory cascades in LPS-activated murine microglia. Thus, our study suggests that the chloroform and hexane fractions of A. holophylla and their terpenoids may be potential drug candidates for drug discovery against LPS-induced neuroinflammation and neuroinflammatory-related neurodegeneration.

Differentiation of Honeydew Honeys from Blossom Honeys and According to Their Botanical Origin by Electrical Conductivity and Phenolic and Sugar Spectra.

Honeydew honey, due to its higher antibacterial and antioxidant activity in comparison to blossom honeys, is in high demand and of interest to consumers. Although a differentiation of blossom honeys from honeydew honeys by way of electrical conductivity is given in many cases, criteria for a differentiation of individual honeydew honeys, such as spruce, fir, and pine, however did not exist. For this reason, 93 authentic honeydew honeys and 63 non-honeydew honeys [35 blossom and 28 nectar-honeydew (mixed)] from 13 different botanical origins were collected within the framework of the current study, and their electrical conductivity and phenolic and sugar profiles were investigated. Results showed that the higher electrical conductivity (≥0.80 mS/cm), the higher protocatechuic acid content (≥3.5 mg/kg), and the higher percentage of the oligosaccharide content (≥120 mg/g) were suitable parameters for the differentiation of authentic coniferous honeydew honeys from non-honeydew honeys; a differentiation. A differentiation of the spruce, fir, and pine honeydew honeys however could not be reached. Through the analysis of 32 carbohydrates (2 mono-, 7 di-, 10 tri-, and 13 higher oligosaccharides) in only one run by high-performance liquid chromatography equipped with an evaporative light scattering detector, marker substances can now be utilized for the classification of individual honeydew honeys. Sugar marker compounds such as α,α-trehalose, melezitose, theanderose, nystose, or maltotetraose in honeydew honeys in combination with chemometrics highlighted the good capability of sugar profiles to discriminate the honeydew honeys both from the non-honeydew honeys and from each other. All in all, a 96.75% correct classification of all studied 156 honey samples was achieved by sugar marker compounds.

Interaction between silver fir (Abies alba) wood water extract and lactobacilli.

Water extract from silver fir (Abies alba) wood represents a rich source of lignans and other phenols that are effective in different pathological conditions, such as diabetes, cardiovascular diseases and psoriasis. Its interaction with the gastrointestinal environment is crucial when the extract is orally administered. In this study we tested the in-vitro interaction between water extract of silver fir wood and ten different Lactobacillus species that are found in the gastrointestinal tract, vagina or are used in food industry. We tested both ways of interaction: 1) the bacterial influence on the chemical composition of the extract and 2) influence of the extract on the bacterial growth. We demonstrated that the extract is compatible with all of the bacteria and does not impair their growth. Furthermore the extract acted as a prebiotic for some bacteria including: L. paracasei, L. acidophilus, L. rhamnosus, L. gasseri, L. crispatus and L. bulgaricus, suggesting that the compounds in the extract can stimulate their growth. However, the ten lactobacilli did not show any chemical changes in lignan metabolism and the production of enterodiol and enterolactone, which are considered the final metabolic products of lignans and are produced by different gut bacteria. This study indicates that the silver fir wood extract is nutritious for some Lactobacillus bacteria and can be used as a prebiotic.

1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi.

Knowledge about the defensive chemistry of coniferous trees has increased in recent years regarding a number of alkaloid compounds; in addition to phenolics and terpenes. Here, we show that Norway spruce (Picea abies (L.) H. Karst.), an important boreal zone tree species; accumulates 1,6-dehydropinidine (2-methyl-6-(2-propenyl)-1,6-piperideine) in its needles and bark. We reanalyzed previously published GC-MS data to obtain a full picture of 1,6-dehydropinidine in P. abies. 1,6-dehydropinidine appeared to especially accumulate in developing spring shoots. We used solid-phase partitioning to collect the alkaloid fraction of the sprouts and thin-layer chromatography to purify 1,6-dehydropinidine. The antibacterial properties of the 1,6-dehydropinidine fraction were tested using a broth microdilution method; with Streptococcus equi subsp. equi as a model organism. Based on our results 1,6-dehydropinidine is common in alkaloid extractions from P. abies (0.4 ± 0.03 mg g-1 dw in mature needles) and it is especially abundant in young spruce shoots (2.7 ± 0.5 mg g-1 dw). Moreover; 1,6-dehydropinidine extracted from P. abies sprouts showed mild antibacterial potential against Streptococcus equi subsp. equi (MIC 55 µg mL-1). The antibacterial activity of a plant compound thought of as an intermediate rather than an end-product of biosynthesis calls for more detailed studies regarding the biological function of these coniferous alkaloids.

Study of the Blood Compatibility of Sulfated Organosolv Lignins Derived from Abies sibirica and Larix sibirica Wood Pulp.

The effects of sulfated organosolv lignins derived from fir (Abies sibirica) and larch (Larix sibirica) (SLf and SLl; 4-3-7.5% sulfur, median-weight molecular mass 2960-4888 Da), on human blood/plasma clotting, platelet aggregation, and erythrocyte hemolysis were studied in vitro. Antithrombin activities of the samples were below 2 U/mg. Specimens of SLf (sulfur content 6.5, 6.6, and 7.5%, molecular weights 3503, 3487, and 3580 Da, respectively) and SLl (4.3 and 6.3%, 2960 and 3497 Da) in a concentration of 0.01 mg/ml did not prolong the blood clotting time, did not provoke human platelet aggregation, did not destroy erythrocyte membranes, and could be used for construction of drug delivery systems. The SLf sample (6.5%, sulfur, 3503 Da) in concentrations from 0.09 to 1.82 mg/ml did not stimulate platelet aggregation, reduced ADP-induced platelet aggregation, and 2-fold prolonged the blood/plasma clotting time 2-fold in comparison with control and could be used for creation of biomaterial with clot-resistant surface.

Silver Fir (Abies alba L.) Polyphenolic Extract Shows Beneficial Influence on Chondrogenesis In Vitro under Normal and Inflammatory Conditions.

Several plant polyphenols have been shown to reduce osteoarthritis symptoms due to their antioxidant, anti-inflammatory and immunomodulatory properties. We investigated the effects of two different polyphenolic extracts (Belinal, Pycnogenol) and two different polyphenols (resveratrol, quercetin) on the chondrogenic potential of bone-derived mesenchymal stem/stromal cells (MSCs) from healthy donors and patients with osteoarthritis. Our main aim was to determine whether Belinal, a commercially available polyphenolic extract from silver fir (Abies alba L.) branches, has comparable chondrogenic potential with the other tested extract and the polyphenols under inflammatory and non-inflammatory conditions. In our study, Belinal promoted significantly greater chondrogenesis compared to the untreated (p = 0.0289) and resveratrol-treated (p = 0.0468) MSCs from patients with hip osteoarthritis under non-inflammatory conditions. Under inflammatory conditions, chondrogenesis was significantly enhanced for MSCs treated with Belinal compared to the control (p = 0.0483). The other extract and the polyphenols did not show any significant effects on chondrogenesis under non-inflammatory or inflammatory conditions. None of the tested extracts and polyphenols showed significant effects on chondrogenesis in healthy donors, under either non-inflammatory or inflammatory conditions. Our data show that Belinal can boost the chondrogenesis of MSCs derived from patients with osteoarthritis, under both non-inflammatory and inflammatory conditions.

Bio Framework-Derived Facile MoO3-NiO-PdO-Pd Nanomaterial for Detoxification of Organic Pollutants.

INTRODUCTION: The catalytic behavior of metal oxide nanomaterials for removal of organic pollutants under dark ambient conditions, without any additional stimulant, is of great interest among the scientific community. METHODS: In this account, a nanomaterial of ternary metal oxides (MoO3-NiO-PdO-Pd) was synthesized via greener approach and was explored for degradation of methyl orange in water environment in dark ambient conditions in comparison with light conditions. The biochemical species of Abies pindrow were treated with aqueous solution of precursor's salt following sol gel synthesis strategy. We further attuned morphology and chemistry of MoO3-NiO-PdO-Pd by incorporating bioactive compounds of A. pindrow. RESULT AND DISCUSSION: The bio-fabricated MoO3-NiO-PdO-Pd revealed outstanding catalytic behavior with 92% degradation of methyl orange within 15 min in the dark at ambient temperature and pressure. Whereas, in the presence of visible light irritation, the catalyst degraded 97% of methyl orange in 15 min. According to the reaction kinetics of degradation, the catalysts illustrated good stability in light (R2=0.93) as well as in dark conditions (R2=0.98). Furthermore, the outstanding reusability and recyclability of the synthesized nanomaterial was observed for four runs of the experiment under dark and light conditions. CONCLUSION: Therefore, A. pindrow-synthesized MoO3-NiO-PdO-Pd nanocatalyst demonstrated significant potential for detoxification of organic pollutants for water remediation.

Identification of Abies sibirica L. Polyprenols and Characterisation of Polyprenol-Containing Liposomes.

The needles of conifer trees are one of the richest sources of natural polyprenols. Polyprenol homologs from Abies sibirica L. lipophilic 80% purified extract were analyzed and quantified. In total, 10 peaks (Prenol-11 to Prenol-20) were observed in the ultra-high-performance liquid chromatography-diode array detector (UHPLC-DAD) chromatogram of Siberian fir with the most abundant compound being Prenol-15 (relative amount 37.23 + 0.56% of the total polyprenol yield). Abies sibirica L. polyprenol solubility and incorporation efficiency into liposomes were studied in various commercially available lecithin mixtures (Phosal IP40, Phosal 75SA, and Lipoid P45). The resulting multilamellar polyprenol liposomes were morphologically characterized by Light and Transmission Electron Microscopy, and the liposome size was discovered to be polymodal with the main peak at 1360 nm (90% of the volume). As polyprenols are fully soluble only in lipids, a liposomal formulation based upon co-solubilization and a modified ethanol injection method of polyprenols into the ethanol-phospholipid system was developed for the entrapment and delivery of polyprenols for potential commercial applications in food supplement and cosmetic industries.