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1.
Braz J Med Biol Res ; 38(7): 1043-51, 2005 Jul.
Article in English | MEDLINE | ID: mdl-16007275

ABSTRACT

The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means +/- SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C: 6.43 +/- 0.21; D: 7.74 +/- 0.45; D + T: 3.86 +/- 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C: 2.30 +/- 0.10; D: 3.28 +/- 0.22; D + T: 1.87 +/- 0.08 nmol cm-2 s-1; t/2, C: 4.75 +/- 0.20; D: 3.52 +/- 0.15; D + T: 5.92 +/- 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C: 8334.21 +/- 112.05; D: 10,217.55 +/- 100.66; D + T: 8478.21 +/- 119.81 microm(2)). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function.


Subject(s)
Acidosis, Renal Tubular/prevention & control , Antioxidants/pharmacology , Diabetes Mellitus, Experimental/urine , Diabetic Nephropathies/prevention & control , Nephrons/drug effects , alpha-Tocopherol/pharmacology , Animals , Glomerular Filtration Rate , Kidney Glomerulus/drug effects , Kidney Glomerulus/metabolism , Kidney Tubules/drug effects , Kidney Tubules/metabolism , Male , Nephrons/metabolism , Rats , Rats, Wistar
2.
Braz. j. med. biol. res ; 38(7)July 2005. tab, graf
Article in English | LILACS | ID: lil-403859

ABSTRACT

The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means ± SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C: 6.43 ± 0.21; D: 7.74 ± 0.45; D + T: 3.86 ± 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C: 2.30 ± 0.10; D: 3.28 ± 0.22; D + T: 1.87 ± 0.08 nmol cm-2 s-1; t/2, C: 4.75 ± 0.20; D: 3.52 ± 0.15; D + T: 5.92 ± 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C: 8334.21 ± 112.05; D: 10,217.55 ± 100.66; D + T: 8478.21 ± 119.81æm²). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function.


Subject(s)
Animals , Male , Rats , Acidosis, Renal Tubular/prevention & control , Antioxidants/pharmacology , Diabetes Mellitus, Experimental/urine , Diabetic Nephropathies/prevention & control , Nephrons/drug effects , alpha-Tocopherol/pharmacology , Glomerular Filtration Rate , Kidney Glomerulus/drug effects , Kidney Glomerulus/metabolism , Kidney Tubules/drug effects , Kidney Tubules/metabolism , Nephrons/metabolism , Rats, Wistar
3.
Urologe A ; 43(4): 429-39, 2004 Apr.
Article in German | MEDLINE | ID: mdl-15085266

ABSTRACT

Alkaline citrates have been used as an efficient therapy in hypocitraturic calcium nephrolithiasis, uric acid lithiasis, cystinuria, and renal tubular acidosis. Furthermore, alkaline citrates are very effective in treating and preventing hyperchloremic metabolic acidosis in patients with urinary diversion. The main physiological effects during urolithiasis therapy have been significant increases in urinary pH, in citrate and potassium, and a decrease in calcium excretion. This paper reviews current indications, therapy modalities, and metaphylactic use reported in the literature and/or recommended by the Deutsche Gesellschaft für Urologie (DGU) and the European Association of Urology (EAU). It is intended to give useful advice for the urologist's daily practice.


Subject(s)
Acidosis, Renal Tubular/drug therapy , Acidosis, Renal Tubular/prevention & control , Alkalies/therapeutic use , Citrates/therapeutic use , Patient Care Management/methods , Patient Care Management/trends , Urinary Calculi/drug therapy , Urinary Calculi/prevention & control , European Union , Germany , Guidelines as Topic , Humans , Patient Care Management/standards , Treatment Outcome
4.
J Pediatr Gastroenterol Nutr ; 25(1): 46-50, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9226526

ABSTRACT

BACKGROUND: Premature infants receiving alimentation with cow's milk formulas are at a considerably high risk of developing incipient late metabolic acidosis, an early stage in the development of manifest late metabolic acidosis. Is it possible to reduce this risk by modification of the composition of a standard formula? METHODS: The mineral composition of a cow's milk preterm formula A was modified (formula B) with the aim of reducing the alimentary load to that of human milk. 160 premature infants were fed either mother's milk (n = 50) or the modified formula B (enriched with sodium and potassium) (n = 110), and their urine pH was tested twice a week. Randomly collected subgroups of infants were studied in detail for nutrient balances. The results were compared with earlier observations of 282 premature infants fed either mother's milk (n = 28) or the standard formula A (n = 254). RESULTS: Incipient late metabolic acidosis was observed in nine of 78 premature infants receiving mother's milk, 53 of 254 premature infants receiving the standard formula A, and only one of 110 premature infants fed the modified formula B. Net acid excretion was 0.58 mmol/kg/day in 11 premature infants receiving alimentation with the modified formula B compared with 1.73 mmol/kg/day in 23 premature infants fed formula A. This reduction was mainly due to an increased alkali excess (sodium + potassium-chloride) in intake and urine. CONCLUSIONS: Reduction of renal acid load with the modified formula B had a preventive effect on the rate of development of incipient late metabolic acidosis in premature infants.


Subject(s)
Acidosis, Renal Tubular/diet therapy , Infant Food , Infant, Premature, Diseases/diet therapy , Infant, Premature/physiology , Infant, Small for Gestational Age/physiology , Urine/chemistry , Acidosis, Renal Tubular/prevention & control , Calcium/metabolism , Calcium/urine , Creatinine/urine , Humans , Hydrogen-Ion Concentration , Infant , Infant Food/adverse effects , Infant Food/analysis , Infant, Newborn , Infant, Premature, Diseases/prevention & control , Male , Minerals/metabolism , Minerals/urine , Nitrogen/metabolism , Nitrogen/urine , Phosphorus/metabolism , Phosphorus/urine , Potassium/metabolism , Potassium/urine , Prospective Studies , Sodium/metabolism , Sodium/urine
6.
Am J Physiol ; 248(4 Pt 2): F513-21, 1985 Apr.
Article in English | MEDLINE | ID: mdl-3985158

ABSTRACT

The metabolic pathogenesis of the complex renal tubular dysfunction of type II renal tubular acidosis and Fanconi's syndrome (RTA II/FS) acutely induced by maleic acid could depend on the occurrence of a positive feedback loop in cells of the proximal renal tubule: impaired mitochondrial oxidation----increased glucose uptake----increased formation and concentration of phosphorylated glycolytic intermediates----limitation on availability of cellular inorganic phosphate----more severely impaired mitochondrial oxidative metabolism. To test this hypothesis we intravenously administered maleic acid both alone and after initiating intravenously administered neutral sodium phosphate, sodium sulfate, or sodium chloride to 10 unanesthetized trained female dogs undergoing water diuresis. We made the following observations: 1) Administration of maleic acid alone predictably induced dose-dependent increments in urine flow (V) and in renal clearance of HCO3-, Na+, K+, and alpha-aminonitrogen and a pronounced increase in the renal clearance and excretion of citrate. 2) Prior phosphate loading, which increased the plasma concentration of phosphate from 2.5 +/- 0.20 to 11.3 +/- 2 mg/dl: a) attenuated the increment in renal clearance of HCO3- by one-half even though the filtered load of bicarbonate was higher by 37%, owing to the higher values of both GFR and plasma bicarbonate concentration that obtained with phosphate loading; b) prevented the increment in renal clearance and excretion of alpha-aminonitrogen; c) significantly attenuated the increments in V and renal clearance of K+; but d) did not affect the increment in renal clearance and excretion of citrate.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Acidosis, Renal Tubular/prevention & control , Fanconi Syndrome/prevention & control , Phosphates/pharmacology , Acidosis, Renal Tubular/chemically induced , Acidosis, Renal Tubular/metabolism , Amino Acids/urine , Animals , Bicarbonates/urine , Chlorides/urine , Dogs , Fanconi Syndrome/chemically induced , Fanconi Syndrome/metabolism , Female , Glomerular Filtration Rate , Injections, Intravenous , Maleates , Nitrogen/urine , Potassium/urine , Sodium/urine , Sulfates/pharmacology
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