Adhesion polypeptides are useful for the prevention of peritoneal dissemination of gastric cancer.

We examined the effect of adhesion polypeptides on the adhesion and invasiveness of gastric cancer cell lines. We previously reported the establishment of an extensively peritoneal-seeding cell line, OCUM-2MD3, from a poorly seeding human scirrhous gastric carcinoma cell line, OCUM-2M. Both alpha2beta1 and alpha3beta1 integrin expression was markedly increased on OCUM-2MD3 cells compared with OCUM-2M cells, and the ability of OCUM-2MD3 cells to bind to the extracellular matrix (ECM) was also significantly higher than that of OCUM-2M cells. The adhesion polypeptides, YIGSR and RGD, and two RGD derivatives significantly inhibited the adhesion of OCUM-2MD3 cells to the submesothelial ECM, while not inhibiting the adhesiveness of OCUM-2M cells and two well differentiated human gastric cell lines, MKN-28 and MKN-74. The YIGSR and RGD peptides also significantly inhibited the invasiveness of OCUM-2MD3 cells. The survival of nude mice with peritoneal dissemination given YIGSR sequence intraperitoneally was obviously longer than that of untreated mice. The survival of mice treated with RGD was also improved, and this effect was increased using the RGD derivatives, poly(CEMA-RGDS) and CM-chitin RGDS. These polypeptides appear to block the binding of integrins, which are expressed on OCUM-2MD3 cells, to the submesothelial ECM, and consequently inhibit peritoneal implantation. The peritoneal injection of adhesion polypeptides may be a new therapy against the dissemination of scirrhous gastric cancer, and may be useful for the prevention of dissemination in high-risk patients.

[Further facts in pleural recurrence prevention by radio-colloidal gold in operated breast cancer]. / Weitere Fakten zur Rezidivprophylaxe der Pleuren beim operierten Brustkrebs mittels kolloidalen Radiogoldes.

The communication deals with endoscopical facts making recognizable that the intrapleural incorporation of colloidal radio-gold after the so-called radical mastectomy corresponds to a preventive-curative act. There is an analysis and proof of lymphogenous nature of the threatening, in it's manifest stage always lethal early and late pleural metastasis. The special and various features of the pleural cavity in postoperative endoscopy are demonstrated as a basis for the following dosage of radio-gold in a preventive-curative sense. Original, spectacular pictures are represented. On the basis of an experience for decades and because of the insufficient results of surgical therapy and conventional radiotherapy, unsatisfactory quoad sanationem et vitam, it must be pointed out that an additional, intrapleural radio-gold therapy is imperative. The application must be done early after operation. The effectiveness of this application is beyond question, since the pioneer-work of J. H. Müller and the ten years results with his preoperative radio-gold infiltration of the breast and my own endoscopically developed intrapleural radio-gold infusion. Both methods make possible true healing and ten years survival. For this reasons I recommend the employ of the more practicable intrapleural infusion on a big collective. The question of a long-term effectiveness of the therapy with fast electrons (betatron) is still to be decided on the ten years parameter. But I don't doubt that radio-gold is superior to all kind of radiotherapy controlling the contralateral pleural dissemination.