ABSTRACT
Adverse drug effects that are uncommon or appear only on chronic administration of a drug may not be detected in clinical trials. This explains the need of strict post-marketing vigilance on drug use. Phenytoin administration has been shown in the literature to be associated with development of neoplasia (benign/malignant). In our knowledge current work represents the first case of pleomorphic-adenoma of sub-mandibular salivary gland developed following chronic phenytoin use. A 40 year old male having a history of head trauma twenty years back, had been on tablet phenytoin 100 mg thrice daily since then. One year back he noticed a small swelling in left sub-mandibular region and gradually increasing in size. FNAC and CECT revealed the diagnosis of pleomorphic-adenoma of sub-mandibular salivary gland. Other causes were ruled out. Surgical excision was performed successfully and continuing follow-up with no recurrence at the end of 6 months. Histo-pathogical examination of the tissue did not show any malignant changes.
Subject(s)
Adenoma, Pleomorphic/chemically induced , Anticonvulsants/adverse effects , Phenytoin/adverse effects , Submandibular Gland Neoplasms/chemically induced , Adenoma, Pleomorphic/diagnosis , Adenoma, Pleomorphic/surgery , Adult , Anticonvulsants/administration & dosage , Follow-Up Studies , Humans , Male , Phenytoin/administration & dosage , Submandibular Gland Neoplasms/diagnosis , Submandibular Gland Neoplasms/surgery , Time FactorsSubject(s)
Adenoma, Pleomorphic/pathology , Antibodies, Monoclonal/adverse effects , Antineoplastic Agents/adverse effects , Carcinoma/pathology , Lung Neoplasms/drug therapy , Adenoma, Pleomorphic/chemically induced , Aged , Carcinoma/chemically induced , Disease Progression , Humans , Lung Neoplasms/pathology , Male , NivolumabABSTRACT
The aim of the study was to determine the immunohistochemical expression of the PCNA, p53 and bcl-2 proteins in pleomorphic adenomas. Nineteen specimens of pleomorphic adenomas were selected for analysis by the streptavidin-biotin-peroxidase method with antibodies againstp53, PCNA and bcl-2 proteins. It was observed weak labeling for p53 in 12 cases (63.1 percent) andforPCNA in 8 (42.1 percent). With respect to the bcl-2 labeling index, o no expression of this protein was detected in 12 cases, corresponding to 63.1 percent of the sample. Based on these findings, it was concluded that p53 and PCNA can favour the proliferative activity of pleomorphic adenomas, whereas bcl-2 probably does not effectively participate in the pathogenesis of this tumor.
El objetivo del estudio fue determinar la expresión inmmunohistoquímica de las proteínas PCNA, p53 y bcl-2 en adenomas pleomórficos. Fueron seleccionados 19 especímenes de adenomas pleomórficos para análisis a través del método de la estreptavidina-biotina-peroxidasa con anticuerpos contra las proteínas p53, PCNA y bcl-2. Fue observada leve marcación para p53 en 12 casos (63,1 por ciento) y para PCNA en 8 (42,1 por ciento). Con relación al índice de marcación para bcl-2, ono fue detectada en 12 casos (63,1 por ciento) expresión de esta proteína. En base a los resultados, se concluyó que las proteínas p53 y PCNA pueden favorecer la actividad proliferativa de adenomas pleomórficos, y por otro lado, la bcl-2 probablemente ono participaría efectivamente de la patogenia de este tumor.
Subject(s)
Humans , Male , Female , Adenoma, Pleomorphic/chemically induced , Adenoma, Pleomorphic/metabolism , Proliferating Cell Nuclear Antigen/isolation & purification , Proliferating Cell Nuclear Antigen/analysis , Proliferating Cell Nuclear Antigen/adverse effects , /adverse effects , Submandibular Gland/anatomy & histology , Submandibular Gland/ultrastructure , Submandibular Gland Neoplasms/blood supply , Submandibular Gland Neoplasms/blood , Submandibular Gland Neoplasms/ultrastructure , Cell ProliferationABSTRACT
Carcinogenesis of 35 ddy male mice submandibular salivary glands were attempted; using implants of a 1 mg (2 mm) prepared pellets of the potent chemical carcinogen 9, 10-dimethyl-1, 2-benzanthracene (DMBA) dry powder without a vehicle or carrier. This method appeared to be easy, fast and effective. Within a period ranging from 7-14 weeks; twenty animals developed epidermoid carcinoma (two of them developed squamous cell carcinoma of covering skin as well), and two animals developed mixed tumors (pleomorphic adenoma like tumor) but neither adenocystic carcinoma nor sarcoma were found, the results were adequately discussed.
Subject(s)
Adenoma, Pleomorphic/chemically induced , Carcinoma, Squamous Cell/chemically induced , Submandibular Gland Neoplasms/chemically induced , 9,10-Dimethyl-1,2-benzanthracene , Adenoma, Pleomorphic/pathology , Animals , Carcinogens , Carcinoma, Squamous Cell/pathology , Disease Models, Animal , Drug Implants , Male , Mice , Submandibular Gland/pathology , Submandibular Gland Neoplasms/pathology , Time FactorsABSTRACT
Carcinogens injected into the excretory canal of submandibular gland of Donryu rats revealed the following histologic changes in salivary glands. 20-Methylcholanthrene induced squamous cell metaplasia, fibrosis in the early stages, and "benign lymphoepithelial lesion"-like pattern after 3 months. Dense hyalinization occurred after 4-5 months with so-called "mixed tumor"-like pattern. In the later stages epidermoid carcinoma and fibrosarcoma were observed. 9, 10-Dimethylbenzanthracene caused degenerative change, metaplasia, fibrosis and cell infiltration, and later carcinoma and sarcoma appeared at a high rate. 4-Nitroquinoline-N-oxide led to dense hyalinization and so-called "mixed tumor"-like pattern was observed in many specimens. N-nitroso-N-methyl urethane and N-methyl-N-nitroso-N'-nitroguanidine revealed metaplastic changes, fibrosis and lymphoid infiltration. Scarlet red induced remarkable infiltration and aggregation of lymphoid cells, showing benign "lymphoepithelial lesion"-like pattern.
