ABSTRACT
Regulatory T cells (Tregs) can be targeted in cancer immunotherapy. A previous study has shown that the chemokine CCL17 and the receptor CCR4 play a role in Treg recruitment in canine urothelial carcinoma. Here, we describe the association of tumor-infiltrating Tregs with CCL17/CCR4 expression in dogs with other carcinomas. In this study, we investigated 23 dogs with mammary carcinoma, 14 dogs with oral squamous cell carcinoma, 16 dogs with pulmonary adenocarcinoma, and 8 healthy control dogs. Immunohistochemistry showed that Foxp3+ Tregs and CCR4+ cells were increased in the tumor tissues of mammary carcinoma, squamous cell carcinoma, and pulmonary adenocarcinoma, when compared with the healthy tissues. The number of CCR4+ cells was associated with that of Foxp3+ Tregs. Double immunofluorescence labeling confirmed that most tumor-infiltrating Foxp3+ Tregs expressed CCR4. In vitro, canine carcinoma cell lines expressed CCL17 mRNA. Quantitative RT-PCR (reverse transcriptase-polymerase chain reaction) showed that CCL17 mRNA expression in canine carcinomas was increased approximately 10- to 25-fold relative to that of healthy tissues. These results suggest that the CCL17/CCR4 axis may drive Treg recruitment in a variety of canine carcinomas. CCR4 blockade may be a potential therapeutic option for tumor eradication through Treg depletion.
Subject(s)
Carcinoma/veterinary , Chemokine CCL17/metabolism , Dog Diseases/pathology , Receptors, CCR4/metabolism , Adenomatosis, Pulmonary/metabolism , Adenomatosis, Pulmonary/veterinary , Animals , Antineoplastic Agents/pharmacology , Biomarkers, Tumor , Carcinoma/metabolism , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/veterinary , Cell Line, Tumor/metabolism , Cell Movement , Dogs , Forkhead Transcription Factors , Mammary Neoplasms, Animal/metabolism , Receptors, CCR4/drug effects , T-Lymphocytes, Regulatory/metabolismABSTRACT
The occurrence of abscess disease, caseous lymphadenitis, and pulmonary adenomatosis in sheep in Denmark is reported for the first time. Subcutaneous abscesses were observed in imported 4- to 5-month-old lambs of the Lacaune breed 10 days after arrival in Denmark. Abscesses were mostly located in the head, neck and shoulder regions close to the regional lymph nodes. Bacteriological examinations revealed growth of Staphylococcus aureus ssp. anaerobius in all animals with subcutaneously located abscesses containing a viscous white-yellow odourless mass. In addition, Corynebacterium pseudotuberculosis was isolated from abscesses in one animal and lesions consistent with pulmonary adenomatosis were found in four animals.
Subject(s)
Abscess/veterinary , Adenomatosis, Pulmonary/veterinary , Lymph Nodes/microbiology , Lymphadenitis/veterinary , Sheep Diseases/microbiology , Staphylococcal Infections/veterinary , Abscess/epidemiology , Abscess/microbiology , Abscess/pathology , Adenomatosis, Pulmonary/epidemiology , Adenomatosis, Pulmonary/microbiology , Adenomatosis, Pulmonary/pathology , Animals , DNA Primers/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/isolation & purification , DNA, Ribosomal/chemistry , DNA, Ribosomal/isolation & purification , Denmark/epidemiology , Disease Outbreaks/veterinary , Lymph Nodes/pathology , Lymphadenitis/epidemiology , Lymphadenitis/microbiology , Lymphadenitis/pathology , Polymerase Chain Reaction/veterinary , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Sheep , Sheep Diseases/epidemiology , Sheep Diseases/pathology , Staphylococcal Infections/epidemiology , Staphylococcal Infections/microbiology , Staphylococcal Infections/pathology , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purificationABSTRACT
Descreve-se, no Rio Grande do Sul, o primeiro caso de adenomatose pulmonar ("jaagsiekte") em um ovino da raça Karakul, fëmea de 2 anos, filha de pais importados da Alemanha. O ovino morreu de acidose lática por sobrecarga alimentar e as lesöes pulmonares representaram achados incidentais. Macroscopicamente encontraram-se duas áreas esbranquiçadas nodulares com aproximadamente 4 e 6cm de diâmetro respectivamente, com bordos irregulares que, microscopicamente, correspondiam a tumoraçäo epitelial bem diferenciada, formando projeçöes papilares para o interior do lúmem alveolar e bronquiolar. Näo foram encontrados indícios de associaçäo com Maedi/Visna através dos achados sorológicos e histopatológicos. O diagnóstico de adenomatose pulmonar, baseou-se na histopatologia.
Subject(s)
Animals , Female , Adenomatosis, Pulmonary/diagnosis , Adenomatosis, Pulmonary/history , Adenomatosis, Pulmonary/veterinary , Sheep Diseases , Retroviridae , SheepSubject(s)
Adenomatosis, Pulmonary/veterinary , Lung Neoplasms/veterinary , Sheep Diseases/etiology , Adenomatosis, Pulmonary/epidemiology , Adenomatosis, Pulmonary/etiology , Animals , Iceland/epidemiology , Israel/epidemiology , Lung Neoplasms/epidemiology , Lung Neoplasms/etiology , Sheep , Sheep Diseases/epidemiology , Sheep Diseases/virology , South Africa/epidemiologyABSTRACT
Sheep respiratory infections appear as differing clinical syndromes. Mild, acute infections are usually due to parainfluenza 3 (PI3) virus. A mild but chronic respiratory problem in lambs under 1 year old is thought to be caused by Mycoplasma ovipneumoniae probably in association with Pasteurella and PI3. Acute bacterial pneumonia usually results from infection with Pasteurella of biotype A. Infection with PI3 can initiate invasion by Pasteurella. Bordetella parapertussis infection has also been implicated. Serotypes of biotype T P. haemolytica cause an acute septicaemia. Stressful management practices may be a predisposing factor. Chronic proliferative pneumonia results from infection by retroviruses of pulmonary adenomatosis or maedi-visna. Both infections have incubation periods extending into years. The former produces fatal tumorous masses in the lungs. Diagnostic tests are being actively sought. Maedi-visna can present as several clinical problems, frequently as an insidious but fatal proliferative pneumonia.
