Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Virology ; 295(1): 108-18, 2002 Mar 30.
Article in English | MEDLINE | ID: mdl-12033770

ABSTRACT

Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised in rabbits. BAV3 E1A, E1B-157R, and E1B-420R were identified as 40, 17, and 47 kDa proteins, and had a half-life of 45-60 min, and 4-6 and 4-6 h, respectively. It appeared that E1A and E1B-157R were phosphorylated at the serine/threonine residues, whereas, E1B 420R was phosphorylated at both the serine/threonine and tyrosine residues. Three cell lines, MDBK-221 (Madin Darby bovine kidney (MDBK) transfected with BAV3 E1), FBK-34 (primary fetal bovine kidney (FBK) cells transfected BAV3 E1), and FBRT-HE1 (bovine fetal retinal (FBRT) cells transfected with HAV5 E1) were isolated and characterized for E1 expression. FBK-34 or FBRT-HE1 supported the replication of an E1A-deleted BAV3 (BAV3DeltaE1AE3) to approximately 1-2 x 10(8) PFU/ml, whereas, the virus titers in MDBK-221 were approximately 10(7) PFU/ml. These cell lines will be useful in generating and growing BAV3 E1-deleted recombinants, and also for studying E1 protein interactions with a number of cellular and/or viral proteins.


Subject(s)
Adenovirus E1 Proteins/chemistry , Mastadenovirus/metabolism , Adenovirus E1 Proteins/genetics , Adenovirus E1 Proteins/metabolism , Adenovirus E1A Proteins/chemistry , Adenovirus E1A Proteins/metabolism , Adenovirus E1B Proteins/chemistry , Adenovirus E1B Proteins/metabolism , Animals , Cattle , Cells, Cultured , Half-Life , Molecular Weight , Phosphorylation , Serine/chemistry , Transfection
2.
Rev Med Virol ; 12(2): 81-92, 2002.
Article in English | MEDLINE | ID: mdl-11921304

ABSTRACT

Simian virus 40 large T antigen, human papilloma virus E7 and adenovirus E1A are all potent oncoproteins that can induce several types of tumours. One of the major functions of these oncoproteins is to interact with the retinoblastoma tumour suppressor protein, Rb, a master switch of the mammalian cell cycle, and to inactivate its function. Rb promotes cell-cycle arrest by recruiting and regulating proteins involved in the transcription of cell proliferation genes. The binding of viral oncoproteins to Rb disrupts the Rb-E2F complex, a central component in the Rb-mediated cell-cycle network. The crystal structures of Rb pocket-viral oncoprotein complexes indicate that the viral proteins recognise a highly conserved region in the Rb pocket through a common motif, LxCxE, and through other unique regions within each viral protein. Although the mechanism of Rb inactivation by viral proteins is not fully understood, information at the atomic level about the interactions between the Rb pocket and viral proteins is providing some insights into how viral proteins dissociate E2F from Rb and thus how they deregulate the cell cycle.


Subject(s)
Antigens, Polyomavirus Transforming/metabolism , Cell Cycle Proteins , DNA-Binding Proteins , Oncogene Proteins, Viral/metabolism , Retinoblastoma Protein/metabolism , Adenovirus E1 Proteins/chemistry , Adenovirus E1 Proteins/metabolism , Antigens, Polyomavirus Transforming/chemistry , E2F Transcription Factors , Humans , Oncogene Proteins, Viral/chemistry , Papillomaviridae/metabolism , Retinoblastoma Protein/chemistry , Transcription Factors/metabolism
3.
Virus Res ; 31(2): 163-86, 1994 Feb.
Article in English | MEDLINE | ID: mdl-8178572

ABSTRACT

The bovine adenovirus type 3 (BAV3) genome was sequenced from the left end to the HindIII site at 11%. This region comprises the entire E1 transcription unit including the open reading frames (ORF) for proteins homologous to the E1A, E1B proteins and protein IX of human adenovirus type 5 (Ad5). A portion of the BAV3 E1A protein showed significant homology with conserved region 3 (CR3), the principal transactivation region of Ad5 E1A. The BAV3 E1A protein also contains a consensus sequence known to be important for interaction with the cellular Rb protein but lacks most of the sequence corresponding to the second exon of Ad5 E1A. Promoter sequences for BAV3 E1B were not defined though the relevant region contains a 35-base pair repeat sequence. Two ORFs define the BAV3 E1B coding unit; one with regions homologous to sequences within the Ad5 E1B 19k protein, and an overlapping ORF with significant homology to the Ad5 E1B 55k protein. The encoded BAV3 E1B proteins of 157 and 420 amino acid residues (R) have predicted unmodified molecular weights of 17,393 and 46,734 respectively. Immediately following the E1B coding region there is a transcription unit containing an SP1 binding site and TATA box followed by an ORF which encodes a protein of 125R and predicted molecular weight of 13,706 with homology to protein IX of Ad5. Five concensus poly A addition sites are located in the 350 base pairs immediately following the protein IX coding region. The homology of sequences in the Ad5 E1A CR3 region and the corresponding BAV3 protein suggested that the BAV3 protein could transactivate certain Ad5 genes normally transactivated by the Ad5 E1A product. Evidence for this hypothesis was obtained in studies in which bovine cells in culture were coinfected with BAV3 and a human adenovirus type 5 (Ad5) recombinant viral vector lacking the E1A region and having a lacZ reporter gene within the E3 region dependent on E1A for its expression. Coinfection resulted in the induction of beta-galactosidase activity and the increased expression of other Ad5 early (E2A 72k) and late (hexon) proteins.


Subject(s)
Adenoviridae/genetics , Adenovirus E1 Proteins/chemistry , Adenovirus E1 Proteins/genetics , Adenoviruses, Human/genetics , Adenoviridae/chemistry , Adenoviruses, Human/chemistry , Amino Acid Sequence , Animals , Base Sequence , Cattle , DNA, Viral/genetics , Genetic Complementation Test , Humans , Molecular Sequence Data , Open Reading Frames , Sequence Homology, Amino Acid , TATA Box
SELECTION OF CITATIONS
SEARCH DETAIL
...