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1.
FASEB J ; 32(4): 2046-2059, 2018 04.
Article in English | MEDLINE | ID: mdl-29196502

ABSTRACT

The investigation of orphan GPCRs (GPRs) has the potential to uncover novel insights into whole animal physiology. In this study, our goal was to determine the renal localization of Gprc5c, a receptor that we previously reported to be highly expressed in murine whole kidney, and to examine physiologic parameters in Gprc5c knockout (KO) mice to gain insight into function. Gprc5c localized to the apical membrane of renal proximal tubules (PTs) in mice, rats, and humans. With the comparison of Gprc5c wild-type (WT) and KO mice, we found that Gprc5c KO mice have altered acid-base homeostasis. Specifically, Gprc5c KO mice have lower blood pH and higher urine pH compared with WT mice, with a reduced level of titratable acids in their urine. In an in vitro GPCR internalization assay, we observed that Gprc5c internalization (an index of activation) was triggered by alkaline extracellular pH. Furthermore, with the use of an in vitro BCECF assay, we observed that Gprc5c increases Na+/H+ exchanger 3 (NHE3) activity at alkaline pH. We also find that the NHE3 activity is reduced in Gprc5c KO mice by 2 photon imaging in seminaphthorhodafluors (SNARF)-4F-loaded kidney sections. NHE3 is a primary contributor to apical transport of H+ in the renal PT. Together, these data imply that Gprc5c modulates the renal contribution to systemic pH homeostasis, at least in part, by taking part in the regulation of NHE3.-Rajkumar, P., Cha, B., Yin, J., Arend, L. J., Paunescu, T. G., Hirabayashi, Y., Donowitz, M., Pluznick, J. L. Identifying the localization and exploring a functional role for Gprc5c in the kidney.


Subject(s)
Kidney Tubules, Proximal/metabolism , Receptors, G-Protein-Coupled/metabolism , Acids/blood , Acids/urine , Alkalies/blood , Alkalies/urine , Animals , HEK293 Cells , Humans , Kidney Tubules, Proximal/physiology , Mice , Mice, Inbred C57BL , Protein Transport , Receptors, G-Protein-Coupled/genetics , Sodium-Hydrogen Exchanger 3/metabolism , Water-Electrolyte Balance
3.
Gene Ther ; 22(5): 374-81, 2015 May.
Article in English | MEDLINE | ID: mdl-25716530

ABSTRACT

We created Na(+)/HCO3(-) cotransporter 1 (NBCe1) p.W516* knock-in mice as a model of isolated proximal renal tubular acidosis showing early lethality associated with severe metabolic acidosis to investigate the therapeutic effects of prenatal alkalization or posttranscriptional control 124 (PTC124). NBCe1(W516*/W516*) mice were treated with non-alkalization (control, n=12), prenatal alkalization postcoitus (prenatal group, n=7) and postnatal alkalization from postnatal day 6 (postnatal group, n=12). Mutation-specific therapy, PTC124 (60 mg kg(-1)) or gentamicin (30 mg kg(-1)), was administered intraperitoneally from postnatal day 6. Blood and urine biochemistry, acid-base analysis, survival rate and renal histology were examined. NBCe1 protein, mRNA abundance and activity ex vivo were assessed after PTC124 and gentamicin treatment. Prenatal group mice had similar initial body weight to wild-type mice and achieved significant weight gain thereafter compared with controls. They had higher serum bicarbonate level (15.5 ± 1.4 vs 5.5 ± 0.1 mmol l(-1), P<0.05) on postnatal day 14 and better renal function, histology and survival rates (60.8 ± 23.5 vs 41.1 ± 15.8 days, P<0.05) than the postnatal group. Compared with the control and gentamicin therapies, PTC124 therapy significantly increased NBCe1 protein abundance despite unchanged mRNA transcription. Only PTC124 therapy significantly increased survival rate and partially rescued NBCe1 activity ex vivo. In NBCe1(W516*/W516*) mice, prenatal alkali therapy achieved higher survival rates and ameliorated organ dysfunction. PTC124 therapy for this nonsense mutation was partially effective in increasing NBCe1 expression and activity.


Subject(s)
Acidosis, Renal Tubular/therapy , Genetic Therapy , Oxadiazoles/therapeutic use , Sodium-Bicarbonate Symporters/genetics , Acidosis, Renal Tubular/genetics , Alkalies/blood , Alkalies/urine , Animals , Gentamicins/administration & dosage , Gentamicins/therapeutic use , Mice , Oxadiazoles/administration & dosage , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sodium-Bicarbonate Symporters/metabolism
4.
Anal Bioanal Chem ; 405(14): 4679-89, 2013 May.
Article in English | MEDLINE | ID: mdl-23494274

ABSTRACT

A sensitive and specific analytical method for cannabidiol (CBD) in urine was needed to define urinary CBD pharmacokinetics after controlled CBD administration, and to confirm compliance with CBD medications including Sativex-a cannabis plant extract containing 1:1 ∆(9)-tetrahydrocannabinol (THC) and CBD. Non-psychoactive CBD has a wide range of therapeutic applications and may also influence psychotropic smoked cannabis effects. Few methods exist for the quantification of CBD excretion in urine, and no data are available for phase II metabolism of CBD to CBD-glucuronide or CBD-sulfate. We optimized the hydrolysis of CBD-glucuronide and/or -sulfate, and developed and validated a GC-MS method for urinary CBD quantification. Solid-phase extraction isolated and concentrated analytes prior to GC-MS. Method validation included overnight hydrolysis (16 h) at 37 °C with 2,500 units ß-glucuronidase from Red Abalone. Calibration curves were fit by linear least squares regression with 1/x (2) weighting with linear ranges (r(2) > 0.990) of 2.5-100 ng/mL for non-hydrolyzed CBD and 2.5-500 ng/mL for enzyme-hydrolyzed CBD. Bias was 88.7-105.3 %, imprecision 1.4-6.4 % CV and extraction efficiency 82.5-92.7 % (no hydrolysis) and 34.3-47.0 % (enzyme hydrolysis). Enzyme-hydrolyzed urine specimens exhibited more than a 250-fold CBD concentration increase compared to alkaline and non-hydrolyzed specimens. This method can be applied for urinary CBD quantification and further pharmacokinetics characterization following controlled CBD administration.


Subject(s)
Alkalies/urine , Cannabidiol/urine , Gas Chromatography-Mass Spectrometry/methods , Glucuronidase/urine , Alkalies/chemistry , Cannabidiol/chemistry , Glucuronidase/chemistry , Humans , Hydrolysis , Reproducibility of Results , Sensitivity and Specificity
5.
J Pediatr Urol ; 9(6 Pt A): 884-9, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23375465

ABSTRACT

INTRODUCTION: Topiramate (TPM), an anti-epileptic drug with >4 million users, increases renal stones in adults. We screened outpatient TPM-treated children without history of stones to estimate the prevalence of renal stones and to characterize urine stone-risk profiles. METHODS: Children taking TPM ≥1 month underwent an interview, renal ultrasound, and spot urine testing in this prospective study. Normal spot urine values were defined as: calcium/creatinine ratio ≤0.20 mg/mg (>12 months) or ≤0.60 mg/mg (≤12 months), citrate/creatinine ratio >0.50 mg/mg, and pH ≤ 6.7. RESULTS: Of 41 patients with average age of 9.2 years (range 0.5-18.7), mean TPM dose of 8.0 mg/kg/day (range 1.4-23.6), and mean treatment duration of 27 months (range 1-112), two (4.9%) had renal stones. The majority of children taking TPM had lithogenic abnormalities on spot urine testing, including 21 (51%) with hypercalciuria, 38 (93%) with hypocitraturia, and 28 (68%) with pH ≥ 6.7. Hypercalciuria and hypocitraturia were independent of TPM dose and duration; urine pH increased with dose. 24-h urine parameters improved in 1 stone-former once TPM was weaned. CONCLUSIONS: Asymptomatic stones were found in 2/41 (4.8%) children taking TPM. Risk factors for stones were present in the spot urine of most children, including hypocitraturia (93%) and hypercalciuria (51%), independent of TPM dose and duration. High urine pH, found in 68%, correlated with TPM dose. Pediatric specialists should be aware of increased risks for stones, hypercalciuria, hypocitraturia, and alkaline urine in children taking TPM.


Subject(s)
Anticonvulsants/adverse effects , Epilepsy/drug therapy , Fructose/analogs & derivatives , Kidney Calculi/chemically induced , Kidney Calculi/epidemiology , Adolescent , Alkalies/urine , Calcium/urine , Child , Child, Preschool , Citric Acid/urine , Creatinine/urine , Epilepsy/epidemiology , Female , Fructose/adverse effects , Humans , Hypercalciuria/chemically induced , Hypercalciuria/epidemiology , Hypercalciuria/urine , Infant , Kidney Calculi/urine , Male , Prevalence , Prospective Studies , Risk Factors , Topiramate
6.
Arch Argent Pediatr ; 110(6): e118-22, 2012 Dec.
Article in Spanish | MEDLINE | ID: mdl-23224315

ABSTRACT

An adolescent with acute lymphoblastic leukemia developed an early and severe tumor lysis syndrome with acute kidney injury after a low and single dose of steroids. Renal dysfunction was attributed primarily to phosphate nephropathy with nephrocalcinosis due to extreme elevations of phosphate in blood. Urinary alkalinization probably contributed to this development. We used peritoneal dialysis with resolution of nephrocalcinosis and normalization of creatinine clearance.


Subject(s)
Acute Kidney Injury/etiology , Acute Kidney Injury/therapy , Peritoneal Dialysis , Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications , Tumor Lysis Syndrome/complications , Acute Kidney Injury/urine , Adolescent , Alkalies/urine , Child , Female , Glucocorticoids/adverse effects , Humans , Methylprednisolone/adverse effects , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Tumor Lysis Syndrome/etiology
7.
Arch. argent. pediatr ; 110(6): e118-e122, dic. 2012. ilus, tab
Article in Spanish | LILACS | ID: lil-662135

ABSTRACT

Una adolescente con leucemia linfoblástica aguda desarrolló un síndrome de lisis tumoral precoz y grave con lesión renal aguda luego de una dosis única y baja de esteroides. La disfunción renal se atribuyó primariamente a una nefropatía por fosfato con nefrocalcinosis debido a elevaciones extremas de este componente en sangre. La alcalinización urinaria probablemente contribuyó a su patogenia. Se utilizó diálisis peritoneal con resolución de la nefrocalcinosis y normalización de la depuración de creatinina.


An adolescent with acute lymphoblastic leukemia developed an early and severe tumor lysis syndrome with acute kidney injury after a low and single dose of steroids. Renal dysfunction was attributed primarily to phosphate nephropathy with nephrocalcinosis due to extreme elevations of phosphate in blood. Urinary alkalinization probably contributed to this development. We used peritoneal dialysis with resolution of nephrocalcinosis and normalization of creatinine clearance.


Subject(s)
Adolescent , Child , Female , Humans , Acute Kidney Injury/etiology , Acute Kidney Injury/therapy , Peritoneal Dialysis , Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications , Tumor Lysis Syndrome/complications , Acute Kidney Injury/urine , Alkalies/urine , Glucocorticoids/adverse effects , Methylprednisolone/adverse effects , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Tumor Lysis Syndrome/etiology
8.
Arch. argent. pediatr ; 110(6): e118-e122, dic. 2012. ilus, tab
Article in Spanish | BINACIS | ID: bin-129068

ABSTRACT

Una adolescente con leucemia linfoblástica aguda desarrolló un síndrome de lisis tumoral precoz y grave con lesión renal aguda luego de una dosis única y baja de esteroides. La disfunción renal se atribuyó primariamente a una nefropatía por fosfato con nefrocalcinosis debido a elevaciones extremas de este componente en sangre. La alcalinización urinaria probablemente contribuyó a su patogenia. Se utilizó diálisis peritoneal con resolución de la nefrocalcinosis y normalización de la depuración de creatinina.(AU)


An adolescent with acute lymphoblastic leukemia developed an early and severe tumor lysis syndrome with acute kidney injury after a low and single dose of steroids. Renal dysfunction was attributed primarily to phosphate nephropathy with nephrocalcinosis due to extreme elevations of phosphate in blood. Urinary alkalinization probably contributed to this development. We used peritoneal dialysis with resolution of nephrocalcinosis and normalization of creatinine clearance.(AU)


Subject(s)
Adolescent , Child , Female , Humans , Acute Kidney Injury/etiology , Acute Kidney Injury/therapy , Peritoneal Dialysis , Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications , Tumor Lysis Syndrome/complications , Acute Kidney Injury/urine , Alkalies/urine , Glucocorticoids/adverse effects , Methylprednisolone/adverse effects , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Tumor Lysis Syndrome/etiology
9.
Nutr J ; 9: 45, 2010 Oct 19.
Article in English | MEDLINE | ID: mdl-20955624

ABSTRACT

BACKGROUND: Increase in the incidence of hyperuricemia associated with gout as well as hypertension, renal diseases and cardiovascular diseases has been a public health concern. We examined the possibility of facilitated excretion of uric acid by change in urine pH by managing food materials. METHODS: Within the framework of the Japanese government's health promotion program, we made recipes which consist of protein-rich and less vegetable-fruit food materials for H+-load (acid diet) and others composed of less protein but vegetable-fruit rich food materials (alkali diet). Healthy female students were enrolled in this consecutive 5-day study for each test. From whole-day collected urine, total volume, pH, organic acid, creatinine, uric acid and all cations (Na+,K+,Ca(2+),Mg(2+),NH4+) and anions (Cl⁻,SO4(2-),PO4⁻) necessary for the estimation of acid-base balance were measured. RESULTS: Urine pH reached a steady state 3 days after switching from ordinary daily diets to specified regimens. The amount of acid generated ([SO4(2-)] +organic acid-gut alkai) were linearly related with those of the excretion of acid (titratable acidity+ [NH4+] - [HCO3⁻]), indicating that H+ in urine is generated by the metabolic degradation of food materials. Uric acid and excreted urine pH retained a linear relationship, where uric acid excretion increased from 302 mg/day at pH 5.9 to 413 mg/day at pH 6.5, despite the fact that the alkali diet contained a smaller purine load than the acid diet. CONCLUSION: We conclude that alkalization of urine by eating nutritionally well-designed food is effective for removing uric acid from the body.


Subject(s)
Acids/urine , Alkalies/urine , Hydrogen-Ion Concentration , Uric Acid/urine , Acid-Base Equilibrium , Creatinine/urine , Diet , Female , Food , Humans , Young Adult
11.
J Nutr ; 134(9): 2355-8, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15333728

ABSTRACT

The mycotoxin ochratoxin A (OA) is a cause of endemic nephropathy in farm animals and humans. Reabsorption of OA along the nephron results from nonionic diffusion and by carrier-mediated mechanisms, indicating that urine alkalinization may help to accelerate OA excretion and thus reduce its toxicity. The aim of the present study was to investigate the effect of a dietary sodium bicarbonate supplementation as a means of increasing urinary pH on the systemic availability and excretion of OA in pigs. Dietary supplementation of 2% sodium bicarbonate significantly increased urinary pH (5.7 +/- 0.2 to 8.3 +/- 0.1) and daily urine volume (1108 +/- 276 to 2479 +/- 912 mL). The systemic availability of OA and its dechloro-analog, Ochratoxin B (OB), calculated as the area under the curve (AUC) was reduced to 75 and 68%, respectively, of the control group (P < 0.05). This effect was due mainly to an accelerated elimination of OA and OB in the urine. The faster renal elimination may be due to reduced reabsorption of the ochratoxins by nonionic diffusion, and other H(+)-dependent mechanisms. Thus, urinary alkalinization may be an efficient means to partially reduce the toxic effects of OA in pigs.


Subject(s)
Alkalies/urine , Kidney/metabolism , Ochratoxins/urine , Swine/urine , Animals , Biological Availability , Dietary Supplements , Hydrogen-Ion Concentration , Mycotoxins/blood , Mycotoxins/urine , Ochratoxins/blood , Sodium Bicarbonate/administration & dosage , Swine/blood , Time Factors
12.
J Toxicol Clin Toxicol ; 42(1): 1-26, 2004.
Article in English | MEDLINE | ID: mdl-15083932

ABSTRACT

This Position Paper was prepared using the methodology agreed by the American Academy of Clinical Toxicology (AACT) and the European Association of Poisons Centres and Clinical Toxicologists (EAPCCT). All relevant scientific literature was identified and reviewed critically by acknowledged experts using set criteria. Well-conducted clinical and experimental studies were given precedence over anecdotal case reports and abstracts were not considered. A draft Position Paper was then produced and presented at the North American Congress of Clinical Toxicology in October 2001 and at the EAPCCT Congress in May 2002 to allow participants to comment on the draft after which a revised draft was produced. The Position Paper was subjected to detailed peer review by an international group of clinical toxicologists chosen by the AACT and the EAPCCT, and a final draft was approved by the boards of the two societies. The Position Paper includes a summary statement (Position Statement) for ease of use, which will also be published separately, as well as the detailed scientific evidence on which the conclusions of the Position Paper are based. Urine alkalinization is a treatment regimen that increases poison elimination by the administration of intravenous sodium bicarbonate to produce urine with a pH > or = 7.5. The term urine alkalinization emphasizes that urine pH manipulation rather than a diuresis is the prime objective of treatment; the terms forced alkaline diuresis and alkaline diuresis should therefore be discontinued. Urine alkalinization increases the urine elimination of chlorpropamide, 2,4-dichlorophenoxyacetic acid, diflunisal, fluoride, mecoprop, methotrexate, phenobarbital, and salicylate. Based on volunteer and clinical studies, urine alkalinization should be considered as first line treatment for patients with moderately severe salicylate poisoning who do not meet the criteria for hemodialysis. Urine alkalinization cannot be recommended as first line treatment in cases of phenobarbital poisoning as multiple-dose activated charcoal is superior. Supportive care, including the infusion of dextrose, is invariably adequate in chlorpropamide poisoning. A substantial diuresis is required in addition to urine alkalinization in the chlorophenoxy herbicides, 2,4-dichlorophenoxyacetic acid, and mecoprop, if clinically important herbicide elimination is to be achieved. Volunteer studies strongly suggest that urine alkalinization increases fluoride elimination, but this is yet to be confirmed in clinical studies. Although urine alkalinization is employed clinically in methotrexate toxicity, currently there is only one study that supports its use. Urine alkalinization enhances diflunisal excretion, but this technique is unlikely to be of value in diflunisal poisoning. In conclusion, urine alkalinization should be considered first line treatment in patients with moderately severe salicylate poisoning who do not meet the criteria for hemodialysis. Urine alkalinization and high urine flow (approximately 600 mL/h) should also be considered in patients with severe 2,4-dichlorophenoxyacetic acid and mecoprop poisoning. Administration of bicarbonate to alkalinize the urine results in alkalemia (an increase in blood pH or reduction in its hydrogen ion concentration); pH values approaching 7.70 have been recorded. Hypokalemia is the most common complication but can be corrected by giving potassium supplements. Alkalotic tetany occurs occasionally, but hypocalcemia is rare. There is no evidence to suggest that relatively short-duration alkalemia (more than a few hours) poses a risk to life in normal individuals or in those with coronary and cerebral arterial disease.


Subject(s)
Alkalies/urine , Poison Control Centers , Practice Guidelines as Topic , Toxicology/methods , Animals , Europe , Humans , Hydrogen-Ion Concentration , Societies, Medical , United States
14.
Urol Res ; 29(5): 295-302, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11762790

ABSTRACT

For many years, urine alkalinization has been one of the cornerstones in the treatment of homozygous cystinuria. Because of the relationship found between the excretion of urinary sodium and cystine, potassium citrate has emerged as the preferred sodium-free alkalizing agent. To evaluate the usefulness of potassium citrate for urine alkalization in cystinuric patients, sodium bicarbonate and potassium citrate were compared in 14 patients (10 on tiopronin treatment and four without treatment with sulfhydryl compounds). The study started with 1 week without the use of any alkalizing agents (Period 0) followed by 2 weeks with sodium bicarbonate (Period 1) and 2 weeks with potassium citrate (Period 2). Urinary pH, volume, excretion of sodium, potassium, citrate and free cystine, as well as the plasma potassium concentration, were recorded. Potassium citrate was shown to be effective as an alkalizing agent and, in this respect, not significantly different from sodium bicarbonate. Even though a normal diet was used, a significant increase in urinary sodium excretion was observed with sodium bicarbonate (Period 1). Urinary potassium and citrate excretion increased with potassium citrate (Period 2). A significant correlation was found between urinary sodium and cystine in the tio-pronin-treated patients. No significant differences in cystine excretion were recorded in Periods 0, 1 and 2. Plasma potassium was significantly higher during Period 2, but only one patient developed a mild hyperkalemia (5.0 mmol/l). The use of potassium citrate for urine alkalization in homozygous cystinuria is effective and can be recommended in the absence of severe renal impairment.


Subject(s)
Alkalies/urine , Cystinuria/drug therapy , Cystinuria/urine , Homozygote , Potassium Citrate/therapeutic use , Sodium Bicarbonate/therapeutic use , Tiopronin/therapeutic use , Adult , Aged , Cystinuria/genetics , Cystinuria/physiopathology , Female , Humans , Hydrogen-Ion Concentration , Male , Middle Aged , Natriuresis/drug effects , Potassium/blood
17.
Urol Radiol ; 9(1): 53-5, 1987.
Article in English | MEDLINE | ID: mdl-3603892

ABSTRACT

A case of alkaline encrusting cystitis is presented, with unusual but characteristic calcifications on plain x-rays and computed tomography. These disappeared completely after adequate treatment.


Subject(s)
Alkalies/urine , Calcinosis/diagnostic imaging , Cystitis/diagnostic imaging , Aged , Humans , Male , Tomography, X-Ray Computed , Urinary Bladder Calculi/therapy
18.
J Pharmacol Exp Ther ; 195(1): 1-7, 1975 Oct.
Article in English | MEDLINE | ID: mdl-241836

ABSTRACT

Two carbonic anhydrase inhibitors, acetazolamide and benzolamide, are capable of increasing the toxicity of sodium salicylate in mice. Beginning at about 2 mg/kg, each of the inhibitors, in combination with a fixed (400 mg/kg) dose of salicylate, generates a dose-mortality curve that reaches a plateau at about 60% deaths at 6 to 8 mg/kg. This effect can be duplicated by 8 to 10% inspired CO2. It appears that the respiratory acidosis secondary to the inhibition of red cell carbonic anhydrase is responsible for the increased toxicity; earlier work by others shows that acidosis increases the concentration of salicylate in the brain. In the treatment of salicylate poisoning by carbonic anhydrase inhibitors, the goal is to alkalinize the urine and increase the excretion of salicylate. With the newer inhibitor, benzolamide, it is possible to dissociate the respiratory acidosis from the renal effect. Maximal alkalinization of the urine is possible with a dose (about 1 mg/kg) below that which generates a respiratory acidosis. With this dose, there is no increase in the early toxicity of salicylate.


Subject(s)
Acetazolamide/toxicity , Benzolamide/pharmacology , Carbon Dioxide/metabolism , Salicylates/toxicity , Thiadiazoles/pharmacology , Acidosis, Respiratory/physiopathology , Alkalies/urine , Animals , Carbon Dioxide/toxicity , Hydrogen-Ion Concentration , Lethal Dose 50 , Male , Mice , Mice, Inbred ICR
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