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1.
Phytochemistry ; 222: 114069, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38548035

ABSTRACT

Seventeen piperidine alkaloids, including 15 previously undescribed 2-substituted-6-(9-phenylnonyl)-piperidine-3,4-diol alkaloids and a previously undescribed 2-substituted-6-(9-phenylnonyl)-piperidine-3-ol alkaloid, were isolated from the leaves of Alocasia macrorrhiza (L.) Schott. Their planar structures and configurations were elucidated based on HR-ESI-MS, 1D and 2D NMR, Snatzke's method, modified Mosher method, single-crystal X-ray crystallography, as well as quantum chemical calculation. It was found that ΔδH5b-H5a can be used to elucidate the relative configuration of 2,3,4,6-tetrasubstituted piperidine, by analyzing the NMR data of 2-substituted-6-(9-phenylnonyl)-piperidine-3,4-diol. Antiproliferative activity was evaluated for all of the alkaloids, and compounds 6-8 showed considerable inhibitory activity against K562 cell line, with the IC50 values of 17.24 ± 1.62, 19.31 ± 0.9 and 18.77 ± 1.09µM, respectively. Furthermore, compounds 6 and 7 exerted an antiproliferative effect by inducing apoptosis.


Subject(s)
Alkaloids , Alocasia , Antineoplastic Agents, Phytogenic , Cell Proliferation , Drug Screening Assays, Antitumor , Piperidines , Plant Leaves , Plant Leaves/chemistry , Alkaloids/pharmacology , Alkaloids/chemistry , Alkaloids/isolation & purification , Humans , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Proliferation/drug effects , Molecular Structure , Piperidines/pharmacology , Piperidines/chemistry , Piperidines/isolation & purification , Alocasia/chemistry , Structure-Activity Relationship , Dose-Response Relationship, Drug , K562 Cells , Crystallography, X-Ray
2.
Chin J Nat Med ; 20(7): 541-550, 2022 Jul.
Article in English | MEDLINE | ID: mdl-35907653

ABSTRACT

The rhizome of giant taro (Alocasia macrorrhiza (L.) Schott), which is a highly adaptable wild plant, is a traditional Chinese herbal medicine. In the current study, the antiproliferative constituents of giant taro were investigated and six new (1-6) and four known piperidine alkaloids (7-10) were isolated from its rhizomes. Their chemical structures and absolute configurations were elucidated using various spectroscopic methods and the Mosher ester method. The isolated alkaloids were screened for the antiproliferative activity through MTT assay. The results indicated that piperidine alkaloids exerted potential antiproliferative activity against HepG2, AGS and MCF-7 tumor cells. Further researches showed that compounds 3-5 dose-dependently decreased the colony formation rate and induced the apoptosis of AGS cells, while compound 4 induced AGS cell death via the proapoptotic pathway. This study demonstrates that the piperidine alkaloids isolated from giant taro exhibit significant antitumor activity, which provides phytochemical evidence for further development and utilization.


Subject(s)
Alkaloids , Alocasia , Alkaloids/analysis , Alkaloids/pharmacology , Alocasia/chemistry , Humans , Piperidines/pharmacology , Plants , Rhizome/chemistry
3.
Article in English | WPRIM (Western Pacific) | ID: wpr-939919

ABSTRACT

The rhizome of giant taro (Alocasia macrorrhiza (L.) Schott), which is a highly adaptable wild plant, is a traditional Chinese herbal medicine. In the current study, the antiproliferative constituents of giant taro were investigated and six new (1-6) and four known piperidine alkaloids (7-10) were isolated from its rhizomes. Their chemical structures and absolute configurations were elucidated using various spectroscopic methods and the Mosher ester method. The isolated alkaloids were screened for the antiproliferative activity through MTT assay. The results indicated that piperidine alkaloids exerted potential antiproliferative activity against HepG2, AGS and MCF-7 tumor cells. Further researches showed that compounds 3-5 dose-dependently decreased the colony formation rate and induced the apoptosis of AGS cells, while compound 4 induced AGS cell death via the proapoptotic pathway. This study demonstrates that the piperidine alkaloids isolated from giant taro exhibit significant antitumor activity, which provides phytochemical evidence for further development and utilization.


Subject(s)
Humans , Alkaloids/pharmacology , Alocasia/chemistry , Piperidines/pharmacology , Plants , Rhizome/chemistry
4.
Molecules ; 25(11)2020 Jun 08.
Article in English | MEDLINE | ID: mdl-32521624

ABSTRACT

Alocasia longiloba, locally known as 'Keladi Candik', has been used traditionally to treat wounds, furuncle and joint inflammations. A. longiloba can be a new source of herbal medicine against hyperuricemia by inhibiting the activity of xanthine oxidase enzyme, the enzyme which is responsible for the development of hyperuricemia in human. Existing xanthine oxidase inhibitors (XOI drugs) show several side effects on gout patients. Therefore, an alternative herbal medicine from plants, with high therapeutic property and free of side effects, are greatly needed. This study was conducted to evaluate XO inhibitory activity, chemical composition, antioxidant activity and GC-MS profile of A. longiloba. Our results showed that ethanolic petiole extract exhibited the highest XO inhibitory activity (70.40 ± 0.05%) with IC50 value of 42.71 µg/mL, followed by ethanolic fruit extracts (61.44 ± 1.24%) with the IC50 value of 51.32 µg/mL. In a parallel study, the phytochemical analysis showed the presence of alkaloid, flavonoid, terpenoids, glycoside and saponin in petiole and fruit extracts, as well as higher total phenolic and flavonoid contents and strong scavenging activity on DPPH and ABTS antioxidant assay. The GC-MS analysis of fruit and petiole extracts revealed the presence of various compounds belonging to different chemical nature, among them are limonen-6-ol, α-DGlucopyranoside, paromomycin, aziridine, phenol, Heptatriacotanol, Phen-1,2,3-dimethyl and Betulin found in ethanolic fruit extract, and Phen-1,4-diol,2,3-dimethyl-, 1-Ethynyl-3,trans(1,1-dimethylethyl), Phenol,2,6-dimethoxy-4-(2-propenyl)- and 7-Methyl-Z-tetradecen-1-olacetate found in ethanolic petiole extract. Some compounds were documented as potent anti-inflammatory and arthritis related diseases by other researchers. In this study, the efficiency of solvents to extract bioactives was found to be ethanol > water, methanol > hexane > chloroform. Together, our results suggest the prospective utilization of fruit and petiole of A. longiloba to inhibit the activity of XO enzyme.


Subject(s)
Alocasia/chemistry , Antioxidants/pharmacology , Enzyme Inhibitors/pharmacology , Gas Chromatography-Mass Spectrometry/methods , Phytochemicals/pharmacology , Plant Extracts/pharmacology , Xanthine Oxidase/antagonists & inhibitors , Phytochemicals/chemistry , Phytochemicals/isolation & purification , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Xanthine Oxidase/metabolism
5.
Int J Radiat Biol ; 95(11): 1529-1542, 2019 11.
Article in English | MEDLINE | ID: mdl-31314632

ABSTRACT

Evaluation of the modulatory effect of ethanolic extract of Alocasia indica tuber (EEAIT) against γ-irradiation induced ovarian and uterine toxicity. Extract preparation was done by 80% hydro-ethanol using Soxhlet apparatus. EEAIT was administered to female Swiss albino mice (n = 5) daily (200 and 400 mg/kg body weight/d) for 7 days before γ-irradiation exposure (2.9 Gy). FSH, LH, estrogen, progesterone, cytokine levels, and oxidative stress parameters were measured after 24 hours of γ-irradiation. Histology, folliculogenesis, viability of granulosa cells, ROS measurement by flow cytometry, western blot of P450scc, P45017A1, 3ß HSD and SF 1 were also performed. In addition, fertility status was assessed by fecundability and fecundity. The results showed that EEAIT exhibit a strong radioprotective activity by reducing the oxidative stress and thereby restored the ovarian and uterine alterations. EEAIT also improved the abnormality in follicle development, restored altered gonadal hormones and cytokines levels, increase the fertility status, reducing ROS level of granulosa cells with increasing granulosa cells viability and steroidogenic enzyme activity as compared to control. So EEAIT showed a radioprotective effect on γ-irradiation induced ovarian and uterine damage. Our results suggested that Alocasia indica tuber can be a potential radioprotector to prevent female infertility.


Subject(s)
Alocasia/chemistry , Ovary/drug effects , Plant Extracts/pharmacology , Radiation Injuries/prevention & control , Radiation-Protective Agents/pharmacology , Uterus/drug effects , Animals , Antioxidants/metabolism , Catalase/metabolism , Cell Survival/radiation effects , Cytokines/metabolism , Ethanol/chemistry , Female , Fertility/radiation effects , Gamma Rays , Granulosa Cells/radiation effects , Malondialdehyde/metabolism , Mice , Nitric Oxide/metabolism , Ovary/radiation effects , Oxidative Stress , Reactive Oxygen Species/metabolism , Superoxide Dismutase/metabolism , Uterus/radiation effects
6.
Int J Biol Macromol ; 113: 681-691, 2018 Jul 01.
Article in English | MEDLINE | ID: mdl-29505868

ABSTRACT

Protease inhibitors from plants play major role in defensive mechanism against various pathogenic organisms. AMTIN from the tubers of Alocasia macrorrhiza has been purified and characterized as multi-functional Kunitz type protease inhibitor. AMTIN is varied from other KTIs by having three different loops specific for binding to trypsin/amylase and subtilisin that are located approximately 30Ǻ away from one another as evidenced from crystallographic efforts. Biochemical studies on AMTIN reveal simultaneous binding of protease/amylase and have been cross validated using in-silico tools to model Amylase - AMTIN - Trypsin complex without any steric clashes. Apart from multi functionality, the remarkable structural and functional stability of AMTIN at high temperature, presence of many phosphorylation, myristoylation and glycosylation sites and molecular docking studies with dengue viral protease (NS2B-NS3) makes this protein interesting. Hence AMTIN can be considered as a template to design effective antivirals against dengue virus.


Subject(s)
Alocasia/chemistry , Plant Extracts/chemistry , Plant Extracts/pharmacology , Protease Inhibitors/chemistry , Protease Inhibitors/pharmacology , Serine Endopeptidases/metabolism , Viral Nonstructural Proteins/antagonists & inhibitors , Amino Acid Sequence , Molecular Docking Simulation , Plant Extracts/metabolism , Protease Inhibitors/metabolism , Protein Conformation , Serine Endopeptidases/chemistry , Viral Nonstructural Proteins/chemistry , Viral Nonstructural Proteins/metabolism
7.
J Ethnopharmacol ; 213: 359-365, 2018 Mar 01.
Article in English | MEDLINE | ID: mdl-29180042

ABSTRACT

Alocasia cucullata, a Chinese herb, has been used as an anticancer treatment in southern China. Phosphatase and tensin (PTEN), is a tumor suppressor gene and the loss of PTEN expression may activate the phosphoinositide-3-kinase (PI3K)/AKT signaling pathway which play a key role in tumors formation and progression. In this study, we evaluated the anti-melanoma effect and the underlying mechanism of 50% ethanolic extract of A. cucullata (EAC) in vitro and in vivo. Using MTT, wound healing, and transwell assays, we found that EAC suppressed the proliferation, migration, and invasion of melanoma cells (B16-F10, A375 and A2058) in a dose-dependent manner. We also found that EAC suppresses B16-F10 tumor growth in a xenografted mouse model. Western blot analysis revealed that the expression level of PTEN was up-regulated, and phosphorylation of PI3K and AKT reduced in B16-F10 cells and tumor tissues after EAC treatment. No significant differences were observed in PI3K and AKT expression. Moreover, immunohistochemistry showed that the number of PTEN-positive cells in tumor tissues increased and that of p-AKT-positive cells decreased with EAC treatment, corroborating the western blot results. Our data reveal that EAC can inhibit malignant melanoma in vitro and in vivo and suggest that its anti-tumor effect is associated with modulation of the PTEN/ PI3K/AKT signaling pathway. In summary, our findings highlight a promising herbal remedy for the treatment of malignant melanoma, which warrants further study.


Subject(s)
Alocasia/chemistry , Drugs, Chinese Herbal/therapeutic use , Melanoma/drug therapy , PTEN Phosphohydrolase/biosynthesis , Phosphatidylinositol 3-Kinases/metabolism , Phytotherapy , Proto-Oncogene Proteins c-akt/metabolism , Animals , Cell Line, Tumor , Cell Movement/drug effects , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Down-Regulation , Humans , Mice , Neoplasm Invasiveness , Phosphorylation , Plant Roots/chemistry , Signal Transduction , Up-Regulation , Xenograft Model Antitumor Assays
8.
Phytochemistry ; 143: 81-86, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28780427

ABSTRACT

Six previously undescribed piperidine alkaloids were isolated from the rhizomes of Alocasia macrorrhiza (L.) Schott. Their structures were elucidated based on 1D and 2D NMR, IR, HR-ESI-MS spectroscopic analysis and the application of a modified Mosher method. All isolated alkaloids were evaluated for cytotoxicity against five human cancer cell lines (CNE-1, Detroit 562, Fadu, MGC-803, and MCF-7) using the MTT method. Only one compound exhibited cytotoxic effects against Detroit 562, Fadu, and MCF-7 cell lines with IC50 values less than 10 µM.


Subject(s)
Alkaloids/isolation & purification , Alocasia/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Drugs, Chinese Herbal/isolation & purification , Piperidines/isolation & purification , Alkaloids/chemistry , Alkaloids/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/pharmacology , Humans , MCF-7 Cells , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Piperidines/chemistry , Piperidines/pharmacology , Rhizome/chemistry
9.
Shokuhin Eiseigaku Zasshi ; 58(1): 32-35, 2017.
Article in Japanese | MEDLINE | ID: mdl-28260730

ABSTRACT

Kuwazuimo (Alocasia odora) and shimakuwazuimo (Alocasia cucullata) are evergreen perennial plants that originated in East Asia. Although inedible, they are occasionally eaten by mistake because they resemble satoimo (Colocasia esculenta), and this has caused food poisoning in Japan. It is not easy to determine the cause of a food poisoning outbreak from the shape or chemical composition when the available sample is small. Therefore, we developed a new primer pair for PCR to identify kuwazuimo and shimakuwazuimo in small samples, based on the internal transcribed spacer (ITS) region of ribosomal DNA. Using PCR with the developed primer pair, we detected all samples of kuwazuimo obtained from the market, while excluding 17 other kinds of crops. The samples were identified as shimakuwazuimo by DNA sequencing of the PCR products. The present PCR method showed high specificity and was confirmed to be applicable to the identification of kuwazuimo and shimakuwazuimo from various crops.


Subject(s)
Alocasia/chemistry , Alocasia/genetics , Food Analysis/methods , Food Contamination/analysis , Polymerase Chain Reaction/methods , Alocasia/poisoning , DNA, Ribosomal , Foodborne Diseases/etiology , Foodborne Diseases/prevention & control , Sensitivity and Specificity , Sequence Analysis, DNA
10.
Fitoterapia ; 117: 126-132, 2017 Mar.
Article in English | MEDLINE | ID: mdl-28161134

ABSTRACT

Five new lignanamides (1-5), and one new monoindole alkaloid (6), along with eight known compounds (7-14) were isolated and identified from the rhizomes of Alocasia macrorrhiza (giant taro). All purified compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells, and the antiproliferative activities against human nasopharyngeal carcinoma epithelial (CNE-1), human gastric carcinoma (MGC-803), and human breast cancer (MCF-7) cell lines by MTT method. Compounds 2, 4, 7 and 8 exhibited significant inhibitory effects on NO production with the IC50 values of 2.35±0.38, 9.20±0.94, 3.45±0.39 and 7.96±0.56µM, respectively. The results suggested the lignanamides and monoindoles might be responsible for the anti-inflammatory activity of giant taro and might be potential anti-inflammatory candidates.


Subject(s)
Alocasia/chemistry , Anti-Inflammatory Agents/chemistry , Indoles/chemistry , Lignans/chemistry , Animals , Anti-Inflammatory Agents/isolation & purification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Line, Tumor , Humans , Indoles/isolation & purification , Lignans/isolation & purification , Lipopolysaccharides , Mice , Molecular Structure , Nitric Oxide/metabolism , RAW 264.7 Cells , Rhizome/chemistry
11.
Z Naturforsch C J Biosci ; 72(1-2): 21-25, 2017 Jan 01.
Article in English | MEDLINE | ID: mdl-27497869

ABSTRACT

An indole alkaloid, 2-(5-hydroxy-1H-indol-3-yl)-2-oxo-acetic acid (1) isolated for the first time from nature, in addition to the nine known compounds 5-hydroxy-1H-indole-3-carboxylic acid methyl ester (2), alocasin B (3), hyrtiosin B (4), α-monopalmitin (5), 1-O-ß-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2(R)-hydroctadecanoyl) amido]-4,8-octadecadiene-1,3-diol (6), 3-epi-betulinic acid (7), 3-epi-ursolic acid (8), ß-sitosterol (9) and ß-sitosterol 3-O-ß-D-glucoside (10) were isolated from the rhizomes of Alocasia macrorrhiza (Araceae). Their structures were elucidated by 1D and 2D NMR spectroscopic data. Of these compounds, 6 exhibited the strongest cytotoxicity against the four tested human cancer cell lines (IC50 of about 10 µM against Hep-2 larynx cancer cells).


Subject(s)
Alocasia/chemistry , Cytotoxins/analysis , Indole Alkaloids/analysis , Alocasia/toxicity , Antineoplastic Agents, Phytogenic/analysis , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Cytotoxins/isolation & purification , Cytotoxins/pharmacology , Drug Screening Assays, Antitumor , Humans , Indole Alkaloids/isolation & purification , Indole Alkaloids/pharmacology , Inhibitory Concentration 50 , Mass Spectrometry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Rhizome/chemistry
12.
J Nat Med ; 70(3): 602-9, 2016 Jul.
Article in English | MEDLINE | ID: mdl-27120176

ABSTRACT

Ten steroids and iridoids were isolated from the tubers of Alocasia cucullata (Lour.) G. Don. Among them, alocasgenin A (1) and alocasgenoside B-C (2-3) were new compounds and the aglycone of compound 1, obtained from the acid hydrolysis of 1, was named alocasgenol (1a). Also, for the first time, tenacigenin B (4), 17ß-tenacigenin-B (5), 3-O-6-deoxy-3-O-methyl-ß-D-allopyranosyl-(1→4)-ß-D-oleandropyranosyl-tenacigenin C (6), marsdenoside A-B (7-8) and tenacigenoside A-B (9-10) were isolated from the genus Alocasia. The chemical structures were elucidated by the extensive analysis of spectral data and compared with the literature. By evaluation of the cytotoxic and tyrosine kinase inhibition, compounds 1-10, 1a and compound 2 showed significant growth inhibition against two tumour cell lines, MGC-803 and HT-29, while compounds 1, 1a, 3, 6 and 8 presented moderate inhibition. Furthermore, compound 2 had the inhibitory property against the enzyme activity biochemically.


Subject(s)
Alocasia/chemistry , Iridoids/chemistry , Protein-Tyrosine Kinases/antagonists & inhibitors , Steroids/chemistry , Cell Line, Tumor , Humans , Molecular Structure
13.
Bioresour Technol ; 217: 252-6, 2016 Oct.
Article in English | MEDLINE | ID: mdl-26897473

ABSTRACT

The study was carried out to investigate the use of a high biomass plant, Pennisetum hydridum, to treat municipal sewage sludge (MSS). An experiment composed of plots with four treatments, soil, fresh sludge, soil-sludge mixture and phyto-treated sludge, was conducted. It showed that the plant could not survive directly in fresh MSS when cultivated from stem cuttings. The experiment transplanting the incubated cutting with nurse medium of P. hydridum in soil and fresh MSS, showed that the plants grew normally in fresh MSS. The pilot experiment of P. hydridum and Alocasia macrorrhiza showed that the total yield and nutrient amount of P. hydridum were 9.2 times and 3.6 times more than that of A. macrorrhiza. After plant treatment, MSS was dried, stabilized and suitable to be landfilled or incinerated, with a calorific value of about 5.6MJ/kg (compared to the initial value of 1.9MJ/kg fresh sludge).


Subject(s)
Alocasia , Biodegradation, Environmental , Biomass , Pennisetum , Sewage/chemistry , Alocasia/chemistry , Alocasia/metabolism , Pennisetum/chemistry , Pennisetum/metabolism
14.
J Nat Prod ; 78(12): 3080-2, 2015 Dec 24.
Article in English | MEDLINE | ID: mdl-26625266

ABSTRACT

Herein is reported a synthesis of alocasin A (1), an alkaloid component of Alocasia macrorrhiza, a herbaceous plant used in folk medicine throughout southern Asia. A double Suzuki-Miyaura cross-coupling reaction between a 3-borylindole and 2,5-dibromopyrazine was used to assemble the heteroaromatic framework of the natural product. Removal of the protecting groups gave a synthetic sample of 1, the spectroscopic data of which matched those in the isolation report of this compound.


Subject(s)
Alkaloids/chemical synthesis , Alocasia/chemistry , Biological Products/chemical synthesis , Alkaloids/chemistry , Biological Products/chemistry , Medicine, Traditional , Molecular Structure
15.
Biomed Res Int ; 2014: 349074, 2014.
Article in English | MEDLINE | ID: mdl-24977149

ABSTRACT

The possible protective role of ethanolic extract of A. indica tuber (EEAIT) in hepatotoxicity and apoptosis of liver caused by alcohol in rats was investigated. Treatment of rats with alcohol (3 g ethanol per kg body weight per day for 15 days intraperitoneally) produced marked elevation of liver biomarkers such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT), and total bilirubin levels which were reduced by EEAIT in a dose-dependent manner. Furthermore, EEAIT improved antioxidant status (MDA, NO, and GSH) and preserved hepatic cell architecture. Simultaneous supplementation with EEAIT significantly restored hepatic catalase (CAT) and superoxide dismutase (SOD) activity levels towards normal. The studies with biochemical markers were strongly supported by the histopathological evaluation of the liver tissue. EEAIT also attenuated apoptosis and necrosis features of liver cell found in immunohistochemical evaluation. HPLC analysis of the extract showed the presence of three major peaks of which peak 2 (RT: 33.33 min) contains the highest area (%) and UV spectrum analysis identified it as flavonoids. It is therefore suggested that EEAIT can provide a definite protective effect against chronic hepatic injury caused by alcohol in rats, which may mainly be associated with its antioxidative effect.


Subject(s)
Alocasia/chemistry , Ethanol/adverse effects , Liver Failure/drug therapy , Liver/drug effects , Plant Extracts/chemistry , Alanine Transaminase/metabolism , Animals , Antioxidants/metabolism , Aspartate Aminotransferases/metabolism , Bilirubin/metabolism , Catalase/metabolism , Chromatography, High Pressure Liquid , Dose-Response Relationship, Drug , Female , Glutathione/metabolism , Immunohistochemistry , Lipid Peroxidation , Liver/enzymology , Liver Failure/chemically induced , Nitric Oxide/metabolism , Plant Tubers/chemistry , Rats , Rats, Wistar , Superoxide Dismutase/metabolism , gamma-Glutamyltransferase/metabolism
16.
Environ Technol ; 34(13-16): 2221-9, 2013.
Article in English | MEDLINE | ID: mdl-24350476

ABSTRACT

Land application of sewage sludge usually leads to increased levels of heavy metals in soil, plants and groundwater. Pre-treatment using plants has been proposed to reduce the contents of heavy metals and water in sludge prior to land application. This study quantified the transfer of Zn, Cd, Pb and major nutrients in a sludge-soil-plant-leachate system during the treatment of sewage sludge. To accomplish this, a two year pot experiment was carried out to collect leachate, mono- and co-cropping of Sedum alfredii and feed crops was conducted in sludge with an under-layer soil support. Sludge phyto-treatment increased Zn and Cd concentrations in the under-layer soil, but not Pb. Specifically, 70%, 70% and 80% of the original Zn, Cd and Pb, respectively, remained in the sludge, while about 40%, 70% and 60% of the original N, P and K remained. Only 3% to 5% of Cd and Zn and < 1% of Pb were transferred into the under-layer soils or leachates, while more than 12% of the N and P were transferred. Co-planting S. alfredii and feed crops led to a significant reduction of heavy metals in leachates when compared with sludge without planting. Overall, sludge leachate is more appropriate than whole sludge for recycling in agriculture since it reduces the chance of heavy metal contamination in the agro-ecosystem; therefore, co-cropping phytotreatment of sludge can be coupled with sludge leachate recycling for crop production and re-collection of the sludge residue for landfilling.


Subject(s)
Biodegradation, Environmental , Metals, Heavy/analysis , Plants/chemistry , Plants/metabolism , Sewage , Soil Pollutants/analysis , Alocasia/chemistry , Alocasia/metabolism , Biomass , Metals, Heavy/metabolism , Sedum/chemistry , Sedum/metabolism , Soil/chemistry , Soil Pollutants/metabolism , Zea mays/chemistry , Zea mays/metabolism
17.
PLoS One ; 8(9): e75328, 2013.
Article in English | MEDLINE | ID: mdl-24086508

ABSTRACT

Chinese herbal medicines have long been used to treat various illnesses by modulating the human immune response. In this study, we investigate the immuno-modulating effect and antitumor activity of Alocasia Cucullata (AC), a Chinese herb traditionally used to treat infection and cancer. We found that the whole water extract of AC roots could significantly attenuate tumor growth in mouse tumor models. The median survival time of the AC-treated mice was 43 days, 16 days longer than that of the control group. Moreover, the AC-treated mice showed substantially higher induction of key antitumor cytokines, such as IL-2, IFN-γ, and TNF-α, indicating that AC may exert antitumor effect by activating antitumor immunity. To further pinpoint the cellular and molecular mechanism of AC, we studied the dose response of a human monocytic cell line, THP-1, to the whole water extract of AC. Treatment of the AC extract significantly induced THP-1 differentiation into macrophage-like cells and the differentiated THP-1 showed expression of specific macrophage surface markers, such as CD11b and CD14, as well as productions of antitumor cytokines, e.g. IFN-γ and TNF-α. Our data thus point to AC as potentially a new, alternative immuno-modulating herbal remedy for anticancer treatment.


Subject(s)
Alocasia/chemistry , Antineoplastic Agents/pharmacology , Drugs, Chinese Herbal/pharmacology , Neoplasms/drug therapy , Phytotherapy/methods , Plant Extracts/pharmacology , Animals , Antineoplastic Agents/immunology , Cell Differentiation/drug effects , Cell Differentiation/immunology , Cell Line , Cytokines/immunology , Dose-Response Relationship, Drug , Drug Discovery , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Humans , Mice , Plant Extracts/immunology , Survival Analysis , Water
18.
J Integr Med ; 11(5): 343-51, 2013 Sep.
Article in English | MEDLINE | ID: mdl-24063782

ABSTRACT

OBJECTIVE: To elucidate potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activities of the ethanol extract of Alocasia indica Schott tuber in different experimental models established in vitro and in vivo. METHODS: In vitro antioxidant activity was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was estimated by using Folin-Ciocalteu's reagent while reducing ability was measured by ferric reducing power assay. In vivo antidiarrheal studies were carried out in mice, and the activity was evaluated in castor oil and magnesium sulfate-induced diarrhea. Disk diffusion assay was utilized to determine antibacterial activity against a number of pathogenic bacterial strains. Acute toxicity test was carried out to measure the safe doses for the extract. RESULTS: In DPPH radical-scavenging assay, the extract exhibited strong radical-scavenging activity with the 50% inhibitory concentration value of 42.66 µg/mL. Total phenolic content was found to be 542.26 mg gallic acid equivalent per 100 g of dried tuber extract, whereas flavonoid content was found to be 4.30 mg quercetin equivalent/g of dried tuber extract. In reducing power assay, the extract showed strong reducing power in a concentration-dependent manner. The extract significantly (P < 0.01) enhanced the latent period and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also lessened gastrointestinal motility in mice. Potential antibacterial activity was exhibited by the extract against all the tested bacterial strains in disk diffusion assay. The 50% lethal concentration against brine shrimp nauplii was 81.09 µg/mL. CONCLUSION: The results demonstrated that the ethanol extract of A. indica has potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activity.


Subject(s)
Alocasia , Plant Extracts/pharmacology , Alocasia/chemistry , Animals , Anti-Bacterial Agents/pharmacology , Antidiarrheals/pharmacology , Antioxidants/pharmacology , Artemia/drug effects , Female , Gastrointestinal Motility/drug effects , Male , Mice , Plant Extracts/toxicity
19.
Chem Pharm Bull (Tokyo) ; 60(5): 670-3, 2012.
Article in English | MEDLINE | ID: mdl-22689406

ABSTRACT

Five new indole alkaloids, alocasins A-E (3-7), together with known hyrtiosin B (1) and hyrtiosulawesin (2) were isolated from Alocasia macrorrhiza (L.) SCHOTT; their structures were elucidated on the basis of spectroscopic data. Compounds 1-7 were in vitro tested for cytostatic activity on human throat cancer (Hep-2), human hepatocarcinoma (Hep-G2), and human nasopharyngeal carcinoma epithelial (CNE) cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method; compounds 2, 3, 6 and 7 showed mild antiproliferative activity against Hep-2 and Hep-G2 whereas compounds 2 and 4 showed gentle antiproliferative activity against CNE.


Subject(s)
Alocasia/chemistry , Antineoplastic Agents, Phytogenic/chemistry , Indole Alkaloids/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/toxicity , Cell Line, Tumor , Cell Proliferation/drug effects , Drug Screening Assays, Antitumor , Hep G2 Cells , Humans , Indole Alkaloids/isolation & purification , Indole Alkaloids/toxicity , Magnetic Resonance Spectroscopy , Rhizome/chemistry
20.
Indian J Exp Biol ; 47(10): 816-21, 2009 Oct.
Article in English | MEDLINE | ID: mdl-20112809

ABSTRACT

Oral administration of hydroalcoholic extract of A. indica (250 and 500 mg/kg) effectively inhibited CCl4 and paracetamol induced changes in the serum marker enzymes, cholesterol, serum protein and albumin in a dose-dependent manner as compared to the normal and the standard drug silymarin-treated groups. Hepatic steatosis, fatty infiltration, hydropic degeneration and necrosis observed in CCl4 and paracetamol-treated groups were completely absent in histology of the liver sections of the animals treated with the extracts. The results suggests that the hydroalcoholic extract of leaves of A. indica possess significant potential as hepatoprotective agent.


Subject(s)
Alocasia/chemistry , Liver/drug effects , Plant Extracts/pharmacology , Plant Leaves/chemistry , Acetaminophen/toxicity , Animals , Ethanol/chemistry , Female , Liver Function Tests , Male , Rats , Rats, Wistar
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