ABSTRACT
Identification of nucleotide sequence of the gene 16S rRNA of cyanobacteria Anabaena variabilis str.21 (Uzb1) exhibits that the strain from root sphere of cotton plants is 99% homologous to a known strain of Anabaena variabilis (EF488831.1). This data confirms the phylogenetic relationship of the strain of cyanobacteria A. variabilis Uzb1 to other described representatives of genus Anabaena as addition to morphological characteristics (presence of akinets (spores) and heterocysts).
Subject(s)
Anabaena variabilis/genetics , Anabaena variabilis/isolation & purification , Genes, Bacterial , RNA, Ribosomal, 16S/genetics , Spores, Bacterial/genetics , Anabaena variabilis/classification , Base Sequence , Gossypium/microbiology , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , RNA, Ribosomal, 16S/classification , RNA, Ribosomal, 16S/isolation & purification , Rhizosphere , Spores, Bacterial/classification , Spores, Bacterial/isolation & purificationABSTRACT
We have identified a homologue of 4-deoxygadusol (core of mycosporine-like amino acids) synthesizing gene (ZP_05036788) from Synechococcus sp. PCC 7335 that was found to have additional functionally unknown N-terminal domain similar to homologues from dinoflagellates based on the ClustalW analysis. Phylogenetic analysis revealed that Synechococcus sp. (ZP_05036788) makes a clade together with dinoflagellates and was closest to the Oxyrrhis marina. This study shows for the first time that N-terminal additional sequences that possess upstream plastid targeting sequence in Heterocapsa triquetra and Karlodinium micrum were already evolved in cyanobacteria, and plastid targeting sequence were evolved later in dinoflagellates after divergence from chloroplast lacking Oxyrrhis marina. Thus, MAAs synthesizing genes were transferred from cyanobacteria to dinoflagellates and possibly Synechococcus sp. PCC 7335 acted as a donor during lateral gene transfer event. In addition, we also tried to mutate 4-deoxygadusol synthesizing gene (YP_324358) of Anabaena variabilis PCC 7937 by homologous recombination, however, all approaches to get complete segregation of the mutants from the wild-type were unsuccessful, showing the essentiality of YP_324358 for A. variabilis PCC 7937.
