Subject(s)
Bacteremia/diagnosis , Gram-Negative Bacterial Infections/diagnosis , Adult , Anaerobiospirillum/drug effects , Anaerobiospirillum/isolation & purification , Anti-Bacterial Agents/pharmacology , Campylobacter/isolation & purification , Drug Resistance, Bacterial , Female , Humans , Lupus Erythematosus, Systemic/complicationsABSTRACT
Anaerobiospirillum succiniciproducens is known as an uncommon cause of diarrhea and bacteremia in humans, usually in immunocompromised hosts. We report four cases of A. succiniciproducens bloodstream infection in different hosts, including a previously healthy man. We describe clinical features, antibiotics susceptibility profile, treatment and outcomes. Strains were identified by 16S rRNA gene sequences which contributed to the extension of our MALDI-TOF MS database.
Subject(s)
Anaerobiospirillum/isolation & purification , Bacteremia/microbiology , Gram-Negative Bacterial Infections/microbiology , Adult , Aged, 80 and over , Anaerobiospirillum/chemistry , Anaerobiospirillum/drug effects , Anaerobiospirillum/genetics , Anti-Bacterial Agents/therapeutic use , Bacteremia/diagnosis , Bacteremia/drug therapy , DNA, Bacterial/genetics , Female , Gram-Negative Bacterial Infections/diagnosis , Gram-Negative Bacterial Infections/drug therapy , Humans , Male , Middle Aged , RNA, Ribosomal, 16S/genetics , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methodsSubject(s)
Anaerobiospirillum/isolation & purification , Anti-Bacterial Agents/therapeutic use , Bacteremia/microbiology , Drug Resistance, Bacterial , Gram-Negative Bacterial Infections/microbiology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Aged, 80 and over , Anaerobiospirillum/drug effects , Bacteremia/drug therapy , Female , Gram-Negative Bacterial Infections/drug therapy , HumansABSTRACT
In order to study the occurrence and co-infection of different species of Campylobacter, enteric Helicobacter and Anaerobiospirillum in dogs and cats and define a possible association between these microrganisms and gastrointestinal disorders, 190 dogs and 84 cats, either healthy or with diarrhea, were sampled between 2002 and 2003. Thirty-three C. upsaliensis, 17 C. jejuni, 2 C. helveticus, 1 C. lari isolates from dogs and 14 C. helveticus, 7 C. jejuni, 6 C. upsaliensis isolates from cats were identified using species-specific PCR and phenotypic tests. Whole cell protein profile analysis, phenotypic tests, PCR-RFLP of gyrB and a phylogenetic study of partial groEL and 16S rRNA sequences were used to identify 37 H. bilis, 22 H. canis and 14 H. cinaedi in dogs and 12 H. canis, 5 H. bilis and 2 H. cinaedi in cats. Whole cell protein profile analysis, phenotypic tests and species-specific PCR of 16S rRNA were used to identify 14 A. succiniciproducens, 12 A. thomasii isolates and one unidentified Anaerobiospirillum sp. isolate in dogs and 3 A. thomasii isolates in cats. Fifty-two animals (19%) were positive for the isolation of more than one genus. No significant statistical correlation was found between any isolates of Campylobacter, Helicobacter or Anaerobiospirillum spp. or the various co-infection rates, and the presence of diarrhea in either dogs or cats. Campylobacter isolates were also tested for antibiotic resistance using the agar dilution method.
