Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 5 de 5
Filter
Add more filters










Database
Language
Publication year range
1.
J Neurosurg ; 89(4): 640-4, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9761060

ABSTRACT

The authors report the first DNA-based diagnosis of Bartonella henselae cultured from a brain lesion in a patient with acquired immune deficiency syndrome. This human immunodeficiency virus-infected patient presented with altered mental status, fever, and diabetes insipidus. Magnetic resonance imaging revealed multifocal parenchymal and leptomeningeal involvement, which was confirmed on studies of tissue biopsy samples. Using the polymerase chain reaction and gene sequencing techniques, the authors definitively demonstrated the presence of B. henselae in the brain tissue biopsy specimen.


Subject(s)
AIDS-Related Opportunistic Infections/diagnosis , Angiomatosis, Bacillary/diagnosis , Bartonella henselae/isolation & purification , Brain Diseases/microbiology , Immunocompromised Host , Polymerase Chain Reaction , AIDS-Related Opportunistic Infections/physiopathology , Adult , Angiomatosis, Bacillary/physiopathology , Arachnoid/microbiology , Bartonella henselae/genetics , Brain Diseases/physiopathology , Cognition/physiology , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Diabetes Insipidus/physiopathology , Fever/physiopathology , Humans , Magnetic Resonance Imaging , Male , Meningitis, Bacterial/diagnosis , Meningitis, Bacterial/physiopathology , Pia Mater/microbiology
3.
Am J Vet Res ; 57(12): 1714-9, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8950424

ABSTRACT

OBJECTIVES: To elucidate kinetics of Bartonella henselae bacteremia and IgG response, evaluate antibiotic therapy, and investigate challenge exposure in cats. ANIMALS: Specific-pathogen-free cats. PROCEDURE: Cats were inoculated with B henselae or B quintana and monitored. Convalescent cats were challenge exposed with B henselae. Amoxicillin, enrofloxacin, erythromycin, and tetracycline HCl were evaluated for effect on B henselae bacteremia. RESULTS: Cats developed B henselae bacteremia within 1 week; bacteremia persisted for longer than 2 months before subsiding spontaneously. IgG antibody titer developed shortly after onset of bacteremia; antibody co-existed with bacteremia for several weeks and remained detectable after bacteremia subsided. Cats inoculated with B quintana remained abacteremic. On challenge exposure to B henselae, cats previously infected with B henselae remained abacteremic; cats previously inoculated with B quintana supported B henselae infection. Tetracycline HCl and erythromycin depressed B henselae bacteremia; however, duration of bacteremia remained similar to that in untreated cats. Obvious signs of illness were not observed. CONCLUSIONS: Long-duration, high-titer B henselae infections were highly reproducible in cats. Convalescent cats were immune to reinfection. B quintana-inoculated cats did not have evidence of infection and were susceptible to B henselae challenge exposure. Antibiotic therapy was incompletely efficacious in terminating cat bacteremia. CLINICAL RELEVANCE: A cat with an inapparent B henselae infection must provisionally be regarded as a possible reservoir for infection for a minimum of 2 to 3 months. Convalescent cats are resistant to reinfection. Usual antibiotic therapy was not completely efficacious. Measurement of IgG antibody can be used to detect past or current infection.


Subject(s)
Angiomatosis, Bacillary/drug therapy , Angiomatosis, Bacillary/veterinary , Anti-Bacterial Agents/therapeutic use , Anti-Infective Agents , Bartonella henselae , Cat Diseases , Fluoroquinolones , Amoxicillin/therapeutic use , Angiomatosis, Bacillary/physiopathology , Animals , Antibodies, Bacterial/blood , Bacteremia/drug therapy , Bacteremia/physiopathology , Bacteremia/veterinary , Bartonella henselae/isolation & purification , Cats , Enrofloxacin , Erythromycin/therapeutic use , Female , Immunoglobulin G/blood , Quinolones/therapeutic use , Tetracycline/therapeutic use , Time Factors
5.
Clin Investig ; 70(2): 89-98, 1992 Feb.
Article in English | MEDLINE | ID: mdl-1600345

ABSTRACT

Vascular disorders comprise a wide range of diverse disease entities. Correspondingly, vessels, and even more so the endothelial which line them, show a remarkable extent of heterogeneous differentiation, e.g. between the blood vascular and lymphatic systems, along the length of the vascular trees, and in the microvascular beds of various organs. The most important morphologic criterion to discriminate between endothelia is continuity (continuous endothelial cell layer and well-formed basement membrane) versus discontinuity (intra- or intercellular gaps and/or reduced or missing basement membrane). Most blood vascular endothelia are of the continuous type, while most sinusoidal and lymphatic endothelia are discontinuous by these criteria. Antigen expression corroborates these morphologic data in that CD31, CD34, and 1F10 antigen are exclusively expressed in continuous endothelia, while MS-1 antigen is preferentially expressed in non-continuous sinusoidal endothelia. In contrast, no specific marker has as yet been described for lymphatic endothelia. Endothelial heterogeneity substantially contributes to the pathogenesis of vascular disorders. For example, in patients with acquired immunodeficiency syndrome the same infectious agent may cause either bacillary angiomatosis (a lobular capillary proliferation) or peliosis (sinusoidal dilatation, endothelial denudation, and development of blood-filled cysts) depending on whether the affected organs have predominantly continuous endothelia or noncontinuous sinusoidal endothelia. Moreover, in Kaposi's sarcoma, it is still an open question of whether the lesion is derived from blood vascular or lymphatic endothelia (Kaposi's sarcoma cells in situ do not express the von Willebrand factor+, PAL-E+, 1F10+ phenotype of mature, resting blood vascular endothelia). It is also unresolved how endothelia of either type may be differentially induced to dedifferentiate and how they are recruited into the lesion. Clearly, knowledge about endothelial heterogeneity is still too incomplete to identify the actual mechanisms and molecules that govern the pathogenesis of vascular disorders (including still others than those mentioned here such as atherosclerosis, diabetic angiopathy, and rheumatoid arthritis) affecting distinct endothelia. Further efforts in antigenic phenotyping and in cell and molecular biology of heterogeneously differentiated endothelia should be made to improve this state of affairs.


Subject(s)
Endothelium, Vascular/physiopathology , HIV Infections/physiopathology , Opportunistic Infections/physiopathology , Vascular Diseases/physiopathology , Angiomatosis, Bacillary/physiopathology , Cell Differentiation/physiology , Humans , Sarcoma, Kaposi/physiopathology
SELECTION OF CITATIONS
SEARCH DETAIL
...