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1.
Plant Sci ; 309: 110935, 2021 Aug.
Article in English | MEDLINE | ID: mdl-34134842

ABSTRACT

Eggplant is rich in anthocyanins, which are thought to be highly beneficial for human health. There is no study on weighted gene co-expression network analysis (WGCNA) of anthocyanin biosynthesis in eggplant. Here, transcriptome data of 33 eggplant pericarp samples treated with light were used for WGCNA to identify significant modules. Total 13000 DEGs and 12 modules were identified, and the most significant module was associated with the secondary metabolites pathways. In addition, the hub gene SmWRKY44 with high connectivity was selected and its function was verified. The expression of SmWRKY44 showed a significant correlation with anthocyanin accumulation in the eggplant peels, leaves, and flowers. SmWRKY44-OE Arabidopsis significantly increased the accumulation of anthocyanins. Yeast two-hybrid and BiFC assays showed that SmWRKY44 could interact with SmMYB1, and it was also found that they could jointly promote the biosynthesis of anthocyanins in eggplant leaves through transient expression analysis. Our work provides a new direction for studying the molecular mechanism of light-induced anthocyanin biosynthesis in eggplant.


Subject(s)
Anthocyanins/biosynthesis , Gene Expression Regulation, Plant , Plant Proteins/metabolism , Solanum melongena/genetics , Transcriptome , Anthocyanins/radiation effects , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Flowers/genetics , Flowers/metabolism , Flowers/radiation effects , Fruit/genetics , Fruit/metabolism , Fruit/radiation effects , Gene Expression , Gene Regulatory Networks , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Leaves/radiation effects , Plant Proteins/genetics , Solanum melongena/metabolism , Solanum melongena/radiation effects , Transcription Factors/genetics , Transcription Factors/metabolism
2.
J Plant Physiol ; 254: 153277, 2020 Nov.
Article in English | MEDLINE | ID: mdl-32979788

ABSTRACT

The absorption of Photosynthetically Active Radiation (PAR) by different foliar pigments defines the amount of energy available for photosynthesis and also the need for photoprotection. Both characteristics reveal essential information about productivity, development, and stress acclimation of plants. Here we present an approach for the estimation of the efficiency by three foliar pigment groups (chlorophylls, carotenoids, and anthocyanins) at capturing light, via the absorption coefficient derived from leaf reflectance spectra. The absorption coefficient (and hence light capture efficiency) of the pigment is quantitatively related to the ratio of light absorbed by each pigment group over the total amount of light absorbed by the leaf. The proposed approach allows discerning the contribution of pigment groups to the overall light absorption, despite the strong interference by other pigments with overlapping absorption spectra. For photosynthetic pigments, like chlorophylls, this is indicative of the energy captured for photosynthesis and hence of potential plant productivity. For photoprotective pigments, like anthocyanins or secondary carotenoids, it gives information about the spectral ranges where their optical screening works best and their screening capacity. In addition, the approach allows the selection of optimal spectral bands where different pigments operate. Such information improves our understanding of the phenological, physiological and photosynthetic dynamics of plants over space and through time, useful for developing better monitoring and management strategies.


Subject(s)
Anthocyanins/radiation effects , Carotenoids/radiation effects , Chlorophyll/radiation effects , Plant Leaves/radiation effects , Anthocyanins/metabolism , Carotenoids/metabolism , Chlorophyll/metabolism , Light , Photosynthesis/drug effects , Plant Leaves/metabolism , Plants/metabolism , Plants/radiation effects
3.
Gene ; 728: 144284, 2020 Feb 20.
Article in English | MEDLINE | ID: mdl-31838251

ABSTRACT

The color of berry skin is an important economic trait of grape, which is determined by the composition and concentration of anthocyanins. The anthocyanin accumulation of grape berry skin is affected by light. In order to further explore the mechanisms of light regulation on anthocyanin accumulation in grape, we detected anthocyanin by UPLC-MS and performed transcriptomic analysis using red grape Vitis vinifera cv. 'Red Globe' as material. In our study, 6 kinds of anthocyanins were detected in the berry skin of 'Red Globe'. The high expression of F3'H genes and the low expression of F3'5'H genes led to the accumulation of dihydroxylated anthocyanins which account for 95% of total anthocyanins. After cluster bagging, the expression of key genes which were related to anthocyanin accumulation was down-regulated, and the concentration of total anthocyanins significantly decreased in 'Red Globe'. However, the anthocyanin composition was not changed. A series of candidate genes which were annotated as HY5, UVR8, PHY, CRY and COL may play important roles in the response and transmission of light signals in grape. And multiple transcription factors genes (1 MYB, 3 bHLH, 2 NAC and 1 ERF) were selected which may be involved in the regulation of light-induced anthocyanin accumulation in grape. The results demonstrated that 'Red Globe' is a typical light-depended grape variety whose anthocyanin synthesis in the berry skin is induced by light. Light-induced anthocyanin synthesis is a complex process involving multiple genes. This investigation provided useful insights into further studies on light-induced anthocyanin accumulation in grape berry skin.


Subject(s)
Anthocyanins/metabolism , Gene Expression Profiling , Gene Expression Regulation, Plant/radiation effects , Light , Plant Proteins/genetics , Vitis/growth & development , Vitis/genetics , Anthocyanins/radiation effects , Color , Vitis/radiation effects
4.
Nat Prod Res ; 32(3): 322-326, 2018 Feb.
Article in English | MEDLINE | ID: mdl-28670916

ABSTRACT

The effects of gamma irradiation (GR) on total phenol, anthocyanin and antioxidant activity were investigated in three different Persian pistachio nuts at doses of 0, 1, 2 and 4 kGy. The antioxidant activity, as determined by FRAP and DPPH methods, revealed a significant increase in the 1-2 kGy dose range. Total phenol content (TPC) revealed a similar pattern or increase in this range. However, when radiation was increased to 4 kGy, TPC in all genotypes decreased. A radiation dose of 1 kGy had no significant effect on anthocyanin content of Kale-Ghouchi (K) and Akbari (A) genotypes, while it significantly increased the anthocyanin content in the Ghazvini (G) genotype. In addition, increasing the radiation to 4 kGy significantly increased the anthocyanin content of K and G genotypes. To conclude, irradiation could increase the phenolic content, anthocyanin and antioxidant activity of pistachio nuts.


Subject(s)
Gamma Rays , Nuts/chemistry , Nuts/radiation effects , Pistacia/chemistry , Pistacia/radiation effects , Anthocyanins/analysis , Anthocyanins/radiation effects , Antioxidants/analysis , Antioxidants/pharmacology , Antioxidants/radiation effects , Phenol/analysis , Phenol/radiation effects , Phenols/analysis , Phenols/radiation effects
5.
Planta ; 246(6): 1069-1082, 2017 Dec.
Article in English | MEDLINE | ID: mdl-28801823

ABSTRACT

MAIN CONCLUSION: Up to 40% of incident light was screened in red Berberis leaves in vivo by anthocyanins, resulting also in up to 40% reduction of light-limited photosynthesis. The biological function of anthocyanins in leaves has been strongly discussed, but the hypothesis of a screening function is favored by most authors. For an evaluation of the function as photoprotective pigments, a quantification of their screening of the mesophyll is important. Here, chlorophyll fluorescence excitation of leaves of a red and a green variety of Berberis thunbergii was used to estimate the extent of screening by anthocyanins at 545 nm and over the whole photosynthetically active wavelength range. Growth at high light (430 µmol m-2 s-1) resulted in 90% screening at 545 nm corresponding to 40-50% screening over the whole wavelength range, depending on the light source. The concomitant reduction of photosynthetic quantum yield was of the same size as the calculated reduction of light reaching the chloroplasts. The induction of anthocyanins in the red variety also enhanced the epoxidation state of the violaxanthin cycle under growth conditions, indicating that red leaves were suffering less from excessive irradiance. Pool sizes of violaxanthin cycle carotenoids indicated a shade acclimation of the light harvesting complexes in red leaves. The observed reduction of internal light in anthocyanic leaves has by necessity a photoprotective effect.


Subject(s)
Anthocyanins/metabolism , Berberis/physiology , Anthocyanins/radiation effects , Berberis/radiation effects , Chlorophyll/metabolism , Chlorophyll/radiation effects , Fluorescence , Light , Photosynthesis/physiology , Photosynthesis/radiation effects , Plant Leaves/physiology , Plant Leaves/radiation effects , Xanthophylls/metabolism , Xanthophylls/radiation effects
6.
Sci Rep ; 7(1): 63, 2017 03 03.
Article in English | MEDLINE | ID: mdl-28246400

ABSTRACT

Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. An annotation analysis revealed several pathways involved in the different responsive stages. The presence of LONG HYPOCOTLY 5, CRY-DASH and a CONSTANS-like transcription factors among the early light-responsive genes indicated the pivotal role of light, especially blue light, in the biological changes that occurred after bag removal. Other light-responsive transcription factors were also identified from the three light-responsive stages. In addition, the light-responsive pattern of anthocyanin biosynthetic genes differed among the biosynthetic steps. Although yeast-one hybrid assay showed that most of the structural genes were regulated by PpMYB10, their different temporal expressive pattern suggested that besides PpMYB10, other light-responsive transcriptional factors were also involved in the regulation of anthocyanin biosynthesis. In summary, our transcriptome analysis provides knowledge of the transcriptional regulatory network operating during light responses, which results in anthocyanin accumulation and other significant physiological changes in red Chinese sand pear peels after bag removal.


Subject(s)
Anthocyanins/biosynthesis , Fruit/metabolism , Light , Pyrus/metabolism , Anthocyanins/radiation effects , Color , Crop Production , Gene Expression Profiling , Pyrus/genetics , Pyrus/radiation effects
7.
Acta Biol Hung ; 67(4): 403-411, 2016 Dec.
Article in English | MEDLINE | ID: mdl-28000505

ABSTRACT

The impact of short-term UV-B treatment on the content of individual flavonoids and photosynthetic pigments in cotyledons and the growth of common buckwheat (Fagopyrum esculentum Moench) seedlings was investigated. Seeds of four common buckwheat cultivars were germinated in darkness over a period of 4 days and acclimatized for 2 days under a 16/8 h light/dark photoperiod at 24/18 °C day/night, and exposure to 100-120 µmol ∙ m-2 ∙ s-1 of photosynthetically active radiation (PAR). Seedlings were divided into three batches, including two batches subjected to different doses of UV-B (5 W ∙ m-2 and 10 W ∙ m-2, one hour per day) for 5 days, and a control group exposed to PAR only. Exposure to UV-B increased anthocyanin levels in the cotyledons of all examined cultivars, it inhibited hypocotyl elongation, but did not affect the content of photosynthetic pigments. Flavone concentrations increased in cv. Red Corolla and Kora, remained constant in cv. Panda and decreased in cv. Hruszowska. Exposure to UV-B decreased rutin levels in cv. Hruszowska, but not in the remaining cultivars. Cultivars Hruszowska, Panda and Kora appeared to be less resistant to UV-B than Red Corolla. Higher resistance to UV-B radiation in Red Corolla can probably be attributed to its higher content of anthocyanins and rutin in comparison with the remaining cultivars.


Subject(s)
Anthocyanins/radiation effects , Carotenoids/radiation effects , Cotyledon/radiation effects , Fagopyrum/radiation effects , Flavones/radiation effects , Flavonols/radiation effects , Seedlings/radiation effects , Ultraviolet Rays , Anthocyanins/metabolism , Carotenoids/metabolism , Chlorophyll/metabolism , Chlorophyll/radiation effects , Cotyledon/metabolism , Fagopyrum/growth & development , Fagopyrum/metabolism , Flavones/metabolism , Flavonoids/metabolism , Flavonoids/radiation effects , Flavonols/metabolism , Hypocotyl/growth & development , Hypocotyl/radiation effects , Phenols/metabolism , Phenols/radiation effects , Rutin/metabolism , Rutin/radiation effects , Seedlings/growth & development
8.
Plant Physiol Biochem ; 103: 120-32, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26990403

ABSTRACT

Light is one of the key environmental factors that affect anthocyanin biosynthesis. However, the underlying molecular mechanism remains unclear, and many problems regarding phenotypic change and corresponding gene regulation have not been solved. In the present study, comparative analyses of light-induced anthocyanin accumulation and gene expression between the ray florets and leaves were performed in Chrysanthemum × morifolium 'Purple Reagan'. After contrasting the variations in the flower color phenotype and relative pigment content, as well as expression patterns of structural and regulator genes responsible for anthocyanin biosynthesis and photoreceptor between different plant organs under light and dark conditions, we concluded that (1) both the capitulum and foliage are key organs responding to light for chrysanthemum coloration; (2) compared with flavones, shading makes a greater decrease on the anthocyanins accumulation; (3) most of the structural and regulatory genes in the light-induced anthocyanin pathway specifically express in the ray florets; and (4) CmCHS, CmF3H, CmF3'H, CmANS, CmDFR, Cm3GT, CmMYB5-1, CmMYB6, CmMYB7-1, CmbHLH24, CmCOP1 and CmHY5 are key genes for light-induced anthocyanin biosynthesis in chrysanthemum ray florets, while on the transcriptional level, the expressions of CmPHYA, CmPHYB, CmCRY1a, CmCRY1b and CmCRY2 are insignificantly changed. Moreover, the inferred comprehensive effect of multiple signals on the accumulation of anthocyanins and transmission channel of light signal that exist between the leaves and ray florets were further discussed. These results further our understanding of the relationship between the gene expression and light-induced anthocyanin biosynthesis, and lay foundations for the promotion of the molecular breeding of novel flower colors in chrysanthemums.


Subject(s)
Anthocyanins/metabolism , Chrysanthemum/genetics , Gene Expression Regulation, Plant , Plant Proteins/genetics , Anthocyanins/radiation effects , Chrysanthemum/metabolism , Chrysanthemum/radiation effects , Color , Flowers/genetics , Flowers/metabolism , Flowers/radiation effects , Light , Organ Specificity , Photoreceptors, Plant/genetics , Photoreceptors, Plant/metabolism , Pigmentation , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Leaves/radiation effects , Plant Proteins/metabolism , Plants, Genetically Modified
9.
Planta ; 243(1): 23-41, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26335854

ABSTRACT

MAIN CONCLUSION: Light exclusion reduces the concentration and modifies the composition of grape anthocyanins, by altering the expression of genes involved in anthocyanin biosynthesis and transport, in a cultivar- and tissue-specific manner. Unlike most grapes, teinturier grapes accumulate anthocyanins both in skin and flesh. However, the concentration and composition of anthocyanins in both tissues differ, providing a valuable system to study tissue-specific regulation of anthocyanin synthesis. Furthermore, little is known about the mechanisms controlling the sensitivity of anthocyanin accumulation to light. Here, light was excluded from Gamay (white-fleshed) and Gamay Fréaux (teinturier mutant) berries throughout berry development. Under light-exposed conditions, the skin of Gamay Fréaux accumulated the highest level of anthocyanins, followed by the skin of Gamay, while the pulp of Gamay Fréaux had much lower anthocyanins than the skins. Network analysis revealed the same order on the number of significant correlations among metabolites and transcripts in the three colored tissues, indicating a higher connectivity that reflects a higher efficiency of the anthocyanin pathway. Compared to light conditions, light exclusion reduced the total amount of anthocyanins, most severely in the skin of Gamay and to a lesser extent in the flesh and skin of Gamay Fréaux. Coordinated decrease in the transcript abundance of structural, regulatory and transporter genes by light exclusion correlated with the reduced anthocyanin concentration in a cultivar- and tissue-specific manner. Moreover, light exclusion increased the ratio of dihydroxylated to trihydroxylated anthocyanins, in parallel with F3'H and F3'5'H transcript amounts. Sugars and ABA only play a limited role in the control of anthocyanin synthesis in the berries, in contrast with what has been described in cell suspensions. This study provides novel insights into the regulation of anthocyanin in wild type and teinturier cultivars.


Subject(s)
Anthocyanins/radiation effects , Fruit/radiation effects , Gene Expression Regulation, Plant/radiation effects , Vitis/radiation effects , Anthocyanins/analysis , Anthocyanins/biosynthesis , Fructose/analysis , Fruit/genetics , Fruit/metabolism , Glucose/analysis , Light , Plant Proteins/genetics , Plant Proteins/metabolism , Secondary Metabolism , Vitis/genetics , Vitis/metabolism
10.
BMC Plant Biol ; 14: 377, 2014 Dec 16.
Article in English | MEDLINE | ID: mdl-25511869

ABSTRACT

BACKGROUND: Light is one of the most significant environmental factors affecting to the accumulation of flavonoids in fruits. The composition of the light spectrum has been shown to affect the production of phenolic compounds during fruit ripening. However, specific information on the biosynthesis of flavonoids in fruits in response to different wavelengths of light is still scarce. In the present study bilberry (Vaccinium myrtillus L.) fruits, which are known to be rich with anthocyanin compounds, were illuminated with blue, red, far-red or white light during the berry ripening process. Following the illumination, the composition of anthocyanins and other phenolic compounds was analysed at the mature ripening stage of fruits. RESULTS: All the three monochromatic light treatments had significant positive effect on the accumulation of total anthocyanins in ripe fruits compared to treatment with white light or plants kept in darkness. The elevated levels of anthocyanins were mainly due to a significant increase in the accumulation of delphinidin glycosides. A total of 33 anthocyanin compounds were detected in ripe bilberry fruits, of which six are novel in bilberry (cyanidin acetyl-3-O-galactose, malvidin acetyl-3-O-galactose, malvidin coumaroyl-3-O-galactose, malvidin coumaroyl-3-O-glucose, delphinidin coumaroyl-3-O-galactose, delphinidin coumaroyl-3-O-glucose). CONCLUSIONS: Our results indicate that the spectral composition of light during berry development has significant effect on the flavonoid composition of ripe bilberry fruits.


Subject(s)
Anthocyanins/metabolism , Flavonoids/biosynthesis , Fruit/radiation effects , Light , Phenols/metabolism , Vaccinium myrtillus/radiation effects , Anthocyanins/radiation effects , Chromatography, High Pressure Liquid , Flavonoids/genetics , Flavonoids/radiation effects , Fruit/growth & development , Fruit/metabolism , Phenols/radiation effects , Polymerase Chain Reaction , Tandem Mass Spectrometry , Vaccinium myrtillus/growth & development , Vaccinium myrtillus/metabolism
11.
J Agric Food Chem ; 61(2): 373-8, 2013 Jan 16.
Article in English | MEDLINE | ID: mdl-23249282

ABSTRACT

Understanding the factors that contribute to the degradation of bioactive compounds during microwave treatment is meaningful for the practical application of this novel technology. The influence of microwave power, energy density, temperature, pH value, and initial concentration of anthocyanins (Acys) on the degradation behavior of malvidin-3-glucoside (Mv-3-glu) and malvidin-3,5-diglucoside (Mv-3,5-diglu) was investigated in this study. Results showed that the degradation of both Acys was accelerated with the increase of microwave power, energy density, temperature, pH value, and initial concentration of Acys. The degradation process of both Acys followed the first-order kinetics model (R² > 0.94), whereas the relationship between Acys degradation and energy density fitted to the logistic model well (R² > 0.98). In addition, Mv-3-glu was more susceptible to the microwave treatment than Mv-3,5-diglu. Compared with heating in a 98 ± 2 °C water bath, both Acys degraded more rapidly under microwave treatment at 100 °C, indicating the occurrence of microwave effect. The results provide a guide for the scientific application of microwave treatment.


Subject(s)
Anthocyanins/radiation effects , Anti-Inflammatory Agents, Non-Steroidal/radiation effects , Anticarcinogenic Agents/radiation effects , Glucosides/radiation effects , Microwaves/adverse effects , Models, Chemical , Dietary Supplements/radiation effects , Food Handling , Food Preservation , Kinetics
12.
J Food Sci ; 76(3): C447-53, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21535813

ABSTRACT

UNLABELLED: Davidson's plum (Davidsonia pruriens, F. Muell.), a native to Australian rainforests, large, crimson-red fruit, which superficially resembles plum, has been commercially cultivated in Australia since 1990s. The current production volume exceeds market demands therefore this study was designed to evaluate the suitability of Davidson's plum extract as a source of anthocyanin-based food colorant. The stability of the Davidson's plum extract towards heat treatment at 95 °C was higher than that of commercial mulberry colorant, but inferior to colorants derived from red cabbage and purple sweetpotato. An addition of a variety of phenolic acids significantly increased color intensity indicating the formation of copigmentation complexes. Commercial chlorogenic acid as well as extract from a native Australian herb rich in chlorogenic acid, Tasmannia pepper leaf (Tasmannia lanceolata, R. Br.), were both tested in model soft drink solutions subjected to light irradiation and heat treatment. In both cases, the addition of the copigment resulted in a lasting increase in color intensity. In conclusion, Davidson's plum extract can successfully be utilized as a source of natural food color. Extract from Tasmania pepper leaf can be used as a co-pigment for Davidson's plum anthocyanins. PRACTICAL APPLICATION: The color properties of an anthocyanin colorant derived from the native Australian fruit Davidson's plum are comparable to those of mulberry, which is currently applied as a food colorant in Australian food products. Utilization of Davidson's plum fruit as a source of natural color will allow the industry to increase the range of natural pigments and will create new opportunities for the emerging native food industry.


Subject(s)
Anthocyanins/analysis , Ferns/chemistry , Food Coloring Agents/analysis , Fruit/chemistry , Pigments, Biological/analysis , Plant Leaves/chemistry , Winteraceae/chemistry , Anthocyanins/chemistry , Anthocyanins/radiation effects , Carbonated Beverages/analysis , Cinnamates/analysis , Cinnamates/chemistry , Food Additives/chemistry , Food Coloring Agents/chemistry , Food Coloring Agents/radiation effects , Hot Temperature/adverse effects , Kinetics , Light/adverse effects , Osmolar Concentration , Phenols/analysis , Phenols/chemistry , Pigmentation , Pigments, Biological/chemistry , Pigments, Biological/radiation effects , Plant Extracts/chemistry , Prunus/chemistry
13.
J Food Sci ; 75(6): E323-9, 2010 Aug 01.
Article in English | MEDLINE | ID: mdl-20722916

ABSTRACT

This study was conducted to evaluate the effect of pulsed electric field (PEF) treatment on anthocyanin extraction from red cabbage using water as a solvent. Mashed cabbage was placed in a batch treatment chamber and subjected to PEF (2.5 kV/cm electric field strength; 15 micros pulse width and 50 pulses, specific energy 15.63 J/g). Extracted anthocyanin concentrations (16 to 889 microg/mL) were determined using HPLC. Heat and light stabilities of the control and PEF-treated samples, having approximately the same initial concentrations, were studied. PEF treatments enhanced total anthocyanin extraction in water from red cabbage by 2.15 times with a higher proportion of nonacylated forms than the control (P < 0.05). The heat and light stabilities of the PEF-treated samples and control samples were not significantly different (P > 0.05). Practical Application: An innovative pretreatment technology, pulsed electric field processing, enhanced total anthocyanin extraction in water from red cabbage by 2.15 times. Manufacturers of natural colors can use this technology to extract anthocyanins from red cabbage efficiently.


Subject(s)
Anthocyanins/isolation & purification , Antioxidants/isolation & purification , Brassica/chemistry , Food Coloring Agents/isolation & purification , Food Technology/methods , Plant Leaves/chemistry , Acylation , Anthocyanins/analysis , Anthocyanins/chemistry , Anthocyanins/radiation effects , Antioxidants/analysis , Antioxidants/chemistry , Antioxidants/radiation effects , Chromatography, High Pressure Liquid , Electrochemical Techniques/instrumentation , Food Coloring Agents/analysis , Food Coloring Agents/chemistry , Food Coloring Agents/radiation effects , Light/adverse effects , Mass Spectrometry , Time Factors
14.
J R Soc Interface ; 6(41): 1193-205, 2009 Dec 06.
Article in English | MEDLINE | ID: mdl-19324677

ABSTRACT

The scientific literature describing the effects of weak magnetic fields on living systems contains a plethora of contradictory reports, few successful independent replication studies and a dearth of plausible biophysical interaction mechanisms. Most such investigations have been unsystematic, devoid of testable theoretical predictions and, ultimately, unconvincing. A recent study, of magnetic responses in the model plant Arabidopsis thaliana, however, stands out; it has a clear hypothesis-that seedling growth is magnetically sensitive as a result of photoinduced radical-pair reactions in cryptochrome photoreceptors-tested by measuring several cryptochrome-dependent responses, all of which proved to be enhanced in a magnetic field of intensity 500 muT. The potential importance of this study in the debate on putative effects of extremely low-frequency electromagnetic fields on human health prompted us to subject it to the 'gold standard' of independent replication. With experimental conditions chosen to match those of the original study, we have measured hypocotyl lengths and anthocyanin accumulation for Arabidopsis seedlings grown in a 500 microT magnetic field, with simultaneous control experiments at 50 microT. Additionally, we have determined hypocotyl lengths of plants grown in 50 microT, 1 mT and approximately 100 mT magnetic fields (with zero-field controls), measured gene (CHS, HY5 and GST) expression levels, investigated blue-light intensity effects and explored the influence of sucrose in the growth medium. In no case were consistent, statistically significant magnetic field responses detected.


Subject(s)
Arabidopsis/metabolism , Cryptochromes/chemistry , Anthocyanins/radiation effects , Arabidopsis/radiation effects , Dose-Response Relationship, Radiation , Electromagnetic Fields , Equipment Design , Hypocotyl/radiation effects , Light , Magnetics , Models, Biological , Models, Statistical , Plant Physiological Phenomena , Seedlings/radiation effects
15.
J Ethnopharmacol ; 120(3): 413-8, 2008 Dec 08.
Article in English | MEDLINE | ID: mdl-18930798

ABSTRACT

AIM OF THE STUDY: A survey was conducted in Lubumbashi city (Democratic Republic of Congo) in order to: (a) identify medicinal plants used by traditional healers in the management of sickle cell anaemia, (b) verify their antisickling activity in vitro, (c) determine the most active plants, and (d) verify if anthocyanins are responsible of the bioactivity and study their photodegradation effect. MATERIALS AND METHODS: The Emmel test was used in vitro, for the antisickling activity assays of aqueous and ethanolic extracts of different parts of these plants when a UV lamp and solar irradiations were used to induce the photodegradation effect. RESULTS: The survey revealed that 13 medicinal plants are used in the treatment of drepanocytosis among which 12 plants exhibited the in vitro antisickling activity for at least one of the used parts or extracts. These plants are Bombax pentadrum, Bougainvillea sp., Byarsocarpus orientalis, Dalberigia bochmintaub, Diplorrhynbchus condolocarpus, Euphorbia heterophylla, Ficus capensis, Harungana madagascariensis, Parinari mobola, Pothmania witfchidii, Syzygium guineense, Temnocalys verdickii and Ziziphus mucronata of which four (Bombax pentadrum, Ficus capensis, Parinari mobola and Ziziphus mucronata) revealed a high antisickling activity. The biological activity of three of these plants is due to anthocyanins. The antisickling activity and photodegradation effect of anthocyanins extracts were studied and minimal concentration of normalization determined. The biological activity of Bombax pentadrum anthocyanins decreased to half of its value after 40 min of irradiation under a lamp emitting at a wavelength of 365 nm and after about 10h of solar irradiation. For Ziziphus mucronata and Ficus capensis, the antisickling activity decreased to half after about 6h under a lamp exposition and after about 50h of solar exposition. CONCLUSION: In vitro Antisickling activity justifies the use of these plants by traditional healers and this activity would be due to anthocyanins. But these natural pigments are instable towards UV-Visible irradiations. The conservation of these plants should then be performed in a shield from the sun radiation.


Subject(s)
Anemia, Sickle Cell/drug therapy , Anthocyanins/therapeutic use , Antisickling Agents/therapeutic use , Photolysis , Phytotherapy , Plant Extracts/therapeutic use , Adolescent , Anemia, Sickle Cell/blood , Anthocyanins/pharmacology , Anthocyanins/radiation effects , Antisickling Agents/pharmacology , Antisickling Agents/radiation effects , Bombax/chemistry , Bombax/radiation effects , Democratic Republic of the Congo , Erythrocytes, Abnormal/drug effects , Ficus/chemistry , Ficus/radiation effects , Humans , Medicine, African Traditional , Plant Extracts/pharmacology , Plant Extracts/radiation effects , Plants, Medicinal/chemistry , Plants, Medicinal/radiation effects , Sunlight/adverse effects , Ziziphus/chemistry , Ziziphus/radiation effects
16.
Biotechnol Lett ; 25(14): 1131-5, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12966999

ABSTRACT

Jasmonic acid altered the accumulation of major anthocyanins in Vitis vinifera cell culture. Peonidin 3-glucoside content at day three was increased from 0.3 to 1.7 mg g(-1) dry cell wt while other major anthocyanins were increased by smaller increments. By day 14, the content of methylated and acylated anthocyanins (peonidin 3-p-coumaroylglucoside and malvidin 3-p-coumaroylglucoside) was 6.3 mg g(-1) DCW, in response to treatment with jasmonic acid, and comprising approximately 45% (w/w) of total anthocyanins. In comparison, the untreated control culture contained 1.2 mg g(-1) DCW which made up approximately 32% (w/w) of total anthocyanins. Light further enhanced anthocyanin accumulation induced by jasmonic acid elicitation. The content of peonidin 3-glucoside at day 3 was 6.6 mg g(-1) DCW, 22-fold higher than control cultures while the content in response to light irradiation alone was 0.6 mg g(-1) DCW. When a highly pigmented cell line was elicited with jasmonic acid total anthocyanins increased from 9.2 to 20.7 mg g(-1) DCW, but there was no change in the anthocyanin composition.


Subject(s)
Anthocyanins/biosynthesis , Cyclopentanes/pharmacology , Glucosides/chemistry , Light , Vitis/chemistry , Anthocyanins/chemistry , Anthocyanins/radiation effects , Cell Culture Techniques , Cell Line , Chromatography, High Pressure Liquid , Glucosides/biosynthesis , Glucosides/isolation & purification , Glucosides/radiation effects , Oxylipins , Pigments, Biological/radiation effects , Vitis/drug effects , Vitis/metabolism , Vitis/radiation effects
17.
Plant Cell Physiol ; 44(6): 565-72, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12826621

ABSTRACT

We report the characterization of a semi-dominant mutation fin5-1 (far-red insensitive 5-1) of Arabidopsis, which was isolated from genetic screening of phytochrome A (phyA) signaling components. Plants with the fin5-1 mutation exhibited a long hypocotyl phenotype when grown under far-red (FR) light, but not under red light. Physiological analyses implied that FIN5 might be differentially involved in diverse responses that are regulated by phyA under continuous FR light. Anthocyanin accumulation, gravitropic response of hypocotyl growth, and FR light-preconditioned blocking of greening were also impaired in the fin5-1 mutant, whereas photoperiodic floral induction was not, if at all, significantly affected. Moreover, light-regulated expression of the CHS, PORA and PsbS genes was attenuated in fin5-1 mutant plants, while the light-induced expression of CAB was normal. The mutation exhibited semi-dominance regarding control of hypocotyl growth in FR light. We suggest that FIN5 defines a novel branch in the network of phyA signaling in Arabidopsis.


Subject(s)
Acyltransferases , Arabidopsis Proteins , Arabidopsis/growth & development , Arabidopsis/radiation effects , Light-Harvesting Protein Complexes , Light , Mutation/genetics , Mutation/radiation effects , Photosystem II Protein Complex , Phytochrome/genetics , Phytochrome/radiation effects , Plant Proteins , Adaptation, Ocular , Anthocyanins/metabolism , Anthocyanins/radiation effects , Arabidopsis/genetics , Flowers/growth & development , Flowers/radiation effects , Gene Expression Regulation, Plant/genetics , Gene Expression Regulation, Plant/radiation effects , Hypocotyl/growth & development , Hypocotyl/radiation effects , Photic Stimulation , Photosynthetic Reaction Center Complex Proteins/genetics , Photosynthetic Reaction Center Complex Proteins/metabolism , Photosynthetic Reaction Center Complex Proteins/radiation effects , Phytochrome A , Proteins/metabolism , Proteins/radiation effects , Signal Transduction/genetics , Signal Transduction/radiation effects
18.
Planta ; 215(5): 745-53, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12244439

ABSTRACT

Mutations in a component of phytochrome A (phyA)-specific light signal transduction, SPA1, result in enhanced responsiveness of Arabidopsis seedlings to red and far-red light. Here, we have examined the effects of spa1 mutations on the two known modes of phyA function, the high-irradiance responses (HIRs) to continuous irradiation with far-red light and the very-low-fluence responses (VLFRs) to inductive pulses of light that establish only a small proportion of active phyA. spa1 mutants exhibited an enhanced VLFR under hourly pulses of far-red light for hypocotyl growth inhibition, cotyledon unfolding, anthocyanin accumulation, block of greening in subsequent white light and negative regulation of phyB signaling. We provide evidence that the phenotype of spa1 mutants in red light is also caused by an increase in the VLFR. Taken together, our results indicate that light-induced hypocotyl growth inhibition in spa1 mutants is primarily due to a VLFR. While wild-type seedlings required hourly pulses of far-red light to induce a VLFR, infrequent irradiation with far-red pulses (every 12 h) was sufficient to induce a strong VLFR of hypocotyl elongation in spa1 mutants. This shows that the effect of the VLFR was more persistent in spa1 mutants than in the wild type. We, therefore, propose that SPA1 has an important function in reducing the persistence of phyA signaling. spa1 mutations also enhanced the HIRs of anthocyanin accumulation and of phyA-mediated responsivity amplification towards phyB. Thus, our results suggest that spa1 mutations amplify both the phyA-mediated VLFR and the HIR.


Subject(s)
Arabidopsis Proteins/metabolism , Photoreceptor Cells , Phytochrome/metabolism , Signal Transduction/physiology , Transcription Factors , Anthocyanins/metabolism , Anthocyanins/radiation effects , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis/radiation effects , Arabidopsis Proteins/genetics , Gene Expression Regulation, Plant/radiation effects , Hypocotyl/genetics , Hypocotyl/growth & development , Hypocotyl/radiation effects , Light , Mutation , Phenotype , Phytochrome/genetics , Phytochrome A , Phytochrome B , Signal Transduction/genetics , Signal Transduction/radiation effects
19.
Planta ; 215(4): 557-64, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12172837

ABSTRACT

Several mutants with altered phytochrome A (phyA) signalling have been identified in screenings under continuous far-red light (FR). The latter protocol could preclude the identification of mutants affected in the signalling pathway that operates even under transient phyA activation, compared to the high-irradiance response (HIR) pathway that requires continuous FR. Since some photomorphogenic mutants show shoot-height phenotypes, the screening was conducted on dwarf mutants of Arabidopsis thaliana (L.) Heynh. from the ABRC stocks grown under hourly FR pulses. The dwarf mutant cp3 (compacta 3) showed normal hypocotyl length and folded cotyledons in darkness but enhanced hypocotyl-growth inhibition and cotyledon unfolding under pulsed FR. The HIR and the response mediated by phyB were not affected. Under pulsed FR, seed germination and blocking of greening upon transfer to white light were enhanced in cp3. PHYA levels were normal in cp3. The phenotype under pulsed FR but not the adult phenotype required phyA. We propose that CP3 is involved in the negative regulation of the signalling pathway that saturates with transient activation of phyA.


Subject(s)
Arabidopsis/growth & development , Photoreceptor Cells , Phytochrome/metabolism , Signal Transduction/physiology , Transcription Factors , Adaptation, Physiological/genetics , Anthocyanins/biosynthesis , Anthocyanins/radiation effects , Arabidopsis/genetics , Arabidopsis/radiation effects , Arabidopsis Proteins , Chlorophyll/biosynthesis , Chlorophyll/radiation effects , Cotyledon/genetics , Cotyledon/growth & development , Cotyledon/radiation effects , Darkness , Gene Expression Regulation, Plant/radiation effects , Hypocotyl/genetics , Hypocotyl/growth & development , Hypocotyl/radiation effects , Light , Mutation , Phenotype , Photoperiod , Photosynthesis/genetics , Photosynthesis/physiology , Photosynthesis/radiation effects , Phytochrome A , Phytochrome B , Seeds/genetics , Seeds/growth & development , Seeds/radiation effects , Signal Transduction/radiation effects
20.
Plant Physiol ; 128(3): 1077-86, 2002 Mar.
Article in English | MEDLINE | ID: mdl-11891262

ABSTRACT

We investigated the role of transcription factors (R, SN, C1, and PL) in the regulation of anthocyanin biosynthesis by different light qualities (white, red, blue, and ultraviolet) and by cytokinin in maize (Zea mays). We analyzed anthocyanin accumulation, structural gene expression, and regulatory gene expression in the seed aleurone and the seedling mesocotyl. In the mesocotyl, white, blue, and ultraviolet-B light strongly induced anthocyanin accumulation and expression of two key structural genes. In contrast, red light had little effect. Cytokinin enhanced the response to light but was not sufficient to induce anthocyanin accumulation in darkness. Plants with the pl-bol3 allele showed high levels of anthocyanin accumulation in response to light, whereas those with the pl-W22 allele did not, demonstrating the importance of pl1 in the light response. The expression of the pl-bol3 gene, encoding an MYB-related transcription factor, was induced by light and enhanced by cytokinin in a very similar manner to the structural genes and anthocyanin accumulation. Expression of the bHLH (basic helix-loop-helix) Sn1-bol3 gene was stimulated by several light qualities, but not enhanced by cytokinin, and was less well correlated with the induction of anthocyanin biosynthesis. In the aleurone, white, red, and blue light were effective in stimulating anthocyanin accumulation and expression of the MYB-related gene C1. The bHLH R gene was constitutively expressed. We conclude that specific members of the MYB-related c1/pl1 gene family play important roles in the regulation of anthocyanin synthesis in maize in response to different light qualities and cytokinin.


Subject(s)
Adenine/analogs & derivatives , Cytokinins/pharmacology , Plant Stems/genetics , Proto-Oncogene Proteins c-myb , Seeds/genetics , Transcription Factors/genetics , Zea mays/genetics , Adenine/pharmacology , Anthocyanins/biosynthesis , Anthocyanins/radiation effects , Benzyl Compounds , DNA-Binding Proteins/genetics , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/radiation effects , Genes, Regulator/genetics , Genes, myb/genetics , Helix-Loop-Helix Motifs/genetics , Kinetin , Light , Multigene Family , Plant Growth Regulators/pharmacology , Plant Proteins/genetics , Plant Stems/metabolism , Purines , Seeds/metabolism , Zea mays/metabolism
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