ABSTRACT
The pyrrolo[2,1-c][1,4]benzodiazepines (PBDs) are a family of sequence-selective DNA minor-groove binding agents that form a covalent aminal bond between their C11-position and the C2-NH2 groups of guanine bases. The first example of a PBD monomer, the natural product anthramycin, was discovered in the 1960s, and the best known PBD dimer, SJG-136 (also known as SG2000, NSC 694501 or BN2629), was synthesized in the 1990s and has recently completed Phase II clinical trials in patients with leukaemia and ovarian cancer. More recently, PBD dimer analogues are being attached to tumor-targeting antibodies to create antibody-drug conjugates (ADCs), a number of which are now in clinical trials, with many others in pre-clinical development. This Review maps the development from anthramycin to the first PBD dimers, and then to PBD-containing ADCs, and explores both structure-activity relationships (SARs) and the biology of PBDs, and the strategies for their use as payloads for ADCs.
Subject(s)
Anthramycin/pharmacology , Antibiotics, Antineoplastic/pharmacology , Antibodies/pharmacology , Benzodiazepines/pharmacology , Leukemia/drug therapy , Ovarian Neoplasms/drug therapy , Pyrroles/pharmacology , Anthramycin/chemical synthesis , Anthramycin/chemistry , Antibiotics, Antineoplastic/chemical synthesis , Antibiotics, Antineoplastic/chemistry , Antibodies/chemistry , Benzodiazepines/chemical synthesis , Benzodiazepines/chemistry , Cell Proliferation/drug effects , Female , Humans , Leukemia/pathology , Molecular Structure , Ovarian Neoplasms/pathology , Pyrroles/chemical synthesis , Pyrroles/chemistryABSTRACT
New anthramycin-type analogues, designated usabamycin A-C (1, 2 and 3), have been isolated from cultures of Streptomyces sp. NPS853, a bacterium found in marine sediments. The structures of the new compounds were established on the basis of extensive spectroscopic analyses including 1D- and 2D-NMR ((1)H-(1)H COSY, HSQC, and HMBC) experiments. The usabamycins show weak inhibition of HeLa cell growth and selective inhibition of serotonin (5-hydroxytrypamine) 5-HT(2B) uptake.
Subject(s)
Actinobacteria/chemistry , Anthramycin/analogs & derivatives , Anthramycin/chemistry , Anthramycin/pharmacology , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Cell Proliferation/drug effects , HeLa Cells , Humans , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Molecular Structure , Serotonin Antagonists/chemical synthesis , Serotonin Antagonists/chemistry , Serotonin Antagonists/pharmacologyABSTRACT
The pyrrolobenzodiazepine monomer DRH-417 is a member of the anthramycin group of anti-tumor antibiotics that bind covalently to the N2 of guanine within the minor groove of DNA. DRH-417 emerged from the EORTC-Drug Discovery Committee and NCI 60 cell line in vitro screening programs as a potent antiproliferative agent with differential sensitivity towards certain cancer types such as melanoma, breast and renal cell carcinoma (mean IC(50) = 3 nM). DRH-417 was therefore tested for in vivo activity. The maximum tolerated dose (MTD) was established as 0.5 mg/kg given i.p. Marked anti-tumor activity was seen in two human renal cell cancers, one breast cancer and a murine colon tumor model (p<0.01). A selective HPLC (LC/MS) analytical method was developed and plasma pharmacokinetics determined. At a dose of 0.5 mg kg(-1), the plasma AUC was 540 nM h (197.1 ng h ml(-1)) and the peak plasma concentration (171 nM [62.4 ng ml(-1)]) occurred at 30 min., reaching doses levels well above those needed for in vitro antiproliferative activity. Genomic profiling of in vivo sensitive tumors revealed that the latter have an activated insulin-like growth factor signaling pathway.
Subject(s)
Antibiotics, Antineoplastic/pharmacology , Benzodiazepines/pharmacology , Pyrroles/pharmacology , Animals , Anthramycin/pharmacology , Antibiotics, Antineoplastic/analysis , Antibiotics, Antineoplastic/pharmacokinetics , Benzodiazepines/analysis , Benzodiazepines/therapeutic use , Cell Proliferation/drug effects , Chromatography, High Pressure Liquid , Gene Expression Profiling , Humans , Mass Spectrometry , Mice , Neoplasm Transplantation , Neoplasms, Experimental/drug therapy , Neoplasms, Experimental/metabolism , Pyrroles/analysis , Pyrroles/therapeutic use , Transplantation, HeterologousABSTRACT
A new 7,8-methylenedioxy analogue (4) of (+)-porothramycin B (2) and its water-soluble sodium bisulfite derivative (15) have been synthesized in high yields and have been shown to exhibit high cytotoxic activities against several tumor cell lines. The new pyrrolo[2,1-c][1,4]benzodiazepine 4 was as effective against the resistant cell lines as against the doxorubicin-sensitive cell lines tested.
Subject(s)
Anthramycin/chemical synthesis , Antineoplastic Agents/chemical synthesis , Doxorubicin/pharmacology , Anthramycin/analogs & derivatives , Anthramycin/chemistry , Anthramycin/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Drug Resistance, Neoplasm , Drug Screening Assays, Antitumor , Humans , Stereoisomerism , Structure-Activity Relationship , Tumor Cells, CulturedABSTRACT
Recent work on a number of different classes of anticancer agents that alkylate DNA in the minor groove is reviewed. There has been much work with nitrogen mustards, where attachment of the mustard unit to carrier molecules can change the normal patterns of both regio- and sequence-selectivity, from reaction primarily at most guanine N7 sites in the major groove to a few adenine N3 sites at the 3'-end of poly(A/T) sequences in the minor groove. Carrier molecules discussed for mustards are intercalators, polypyrroles, polyimidazoles, bis(benzimidazoles), polybenzamides and anilinoquinolinium salts. In contrast, similar targeting of pyrrolizidine alkylators by a variety of carriers has little effect of their patterns of alkylation (at the 2-amino group of guanine). Recent work on the pyrrolobenzodiazepine and cyclopropaindolone classes of natural product minor groove binders is also reviewed.
Subject(s)
Alkylating Agents/chemistry , Alkylating Agents/metabolism , DNA/metabolism , Netropsin/analogs & derivatives , Alkylating Agents/pharmacology , Animals , Anthramycin/chemistry , Anthramycin/metabolism , Anthramycin/pharmacology , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/metabolism , Antibiotics, Antineoplastic/pharmacology , Benzofurans/chemistry , Benzofurans/metabolism , Benzofurans/pharmacology , Bisbenzimidazole/chemistry , Bisbenzimidazole/metabolism , Chlorambucil/chemistry , Chlorambucil/metabolism , Cyclohexanecarboxylic Acids/chemistry , Cyclohexanecarboxylic Acids/metabolism , Cyclohexanecarboxylic Acids/pharmacology , Cyclohexenes , DNA/chemistry , DNA/drug effects , Distamycins/chemistry , Distamycins/metabolism , Duocarmycins , Humans , Indoles/chemistry , Indoles/metabolism , Indoles/pharmacology , Netropsin/chemistry , Netropsin/metabolism , Nitrogen Mustard Compounds/chemistry , Nitrogen Mustard Compounds/metabolism , Nitrogen Mustard Compounds/pharmacology , Pyrroles/chemistry , Pyrroles/metabolism , Structure-Activity RelationshipABSTRACT
Interduplex crosslinks by a bifunctional anthramycin DNA crosslinker produced triradial and quadriradial chromosomes. The crosslinker alkylates guanine at N-2. Bovine chromosomes contain GC-rich density satellite DNAs at the centromeric heterochromatin and is the basis for the formation of triradial and quadriradial chromosomes at the centromeres. The in situ crosslinking of interphase chromosomes indicates that the distance between centromeres is 17.5 A. We conclude that the nuclear matrix associated DNA in the centromeric heterochromatin of interphase chromosomes are positioned close enough for crosslinking to occur. We propose a model for the generation of triradial and quadriradial chromosomes based upon the number of interduplex crosslinks between two chromosomes.
Subject(s)
Anthramycin/pharmacology , Chromosome Aberrations , Cross-Linking Reagents/pharmacology , DNA, Satellite/drug effects , Animals , Cattle , Cell Line , In Situ Hybridization, Fluorescence , KaryotypingABSTRACT
It is generally accepted that neoplastic transformation is related to genes alteration or oncogene activation. In particular, DNA minor groove binding drugs have been extensively studied through the years in order to influence the regulation of gene expression by means of specific interactions with DNA bases moieties. Pyrrolo[2,1-c],[1,4].benzodiazepines (PBDs), CC-1065 and distamycins are three classes of minor groove binders which showed interesting cytotoxicity profiles, refined through already reviewed processes of SAR studies. Among the modifications to the three families of antitumor compounds, heterocyclic substitutions have been extensively applied by many groups in order to either modify the reactivity profile or introduce extra interactions within the minor groove, thus changing the binding site or modulating the binding sequence. The updated material related to these modifications has been rationalised and ordered to offer an overview of the argument.
Subject(s)
Alkylating Agents/chemistry , Anthramycin/analogs & derivatives , Antineoplastic Agents/chemistry , Drug Design , Heterocyclic Compounds, 3-Ring/chemistry , Alkylating Agents/metabolism , Alkylating Agents/pharmacology , Anthramycin/chemistry , Anthramycin/pharmacology , Antineoplastic Agents/metabolism , Antineoplastic Agents/pharmacology , Binding Sites , Chemistry, Pharmaceutical , DNA Adducts , Heterocyclic Compounds, 3-Ring/metabolism , Heterocyclic Compounds, 3-Ring/pharmacology , Molecular Structure , Structure-Activity RelationshipABSTRACT
The interactions of several pyrrolo[2, 1-c][1,4]benzodiazepine (PBD) antitumor antibiotics with linearized plasmid pGEM-2-N-ras DNA have been analyzed by quantitative in vitro transcription (QIVT) and in vitro transcription footprinting (IVTF) methods. A concentration-dependent inhibitory effect of the PBDs on transcription is observed using both techniques. The rank order for overall inhibition of transcription by the QIVT method is found to be: sibiromycin > tomaymycin > anthramycin > DC-81 > neothramycin, whereas the IVTF experiments show a different ranking: sibiromycin > anthramycin > neothramycin > tomaymycin. In addition, stimulation of transcription was observed at low PBD concentrations in both the QIVT and IVTF experiments. These results demonstrate unequivocally that the formation of PBD-DNA adducts at AGA-5' base sequences on the transcribed strand results in transcription blockage for all PBDs examined. Furthermore, the sequence of flanking base pairs appears to influence the degree of blocking, with the sequences ACAGAAA-5', AAAGATG-5', AGAGATA-5', and CAAGAAC-5' providing the most pronounced blocks for all PBDs studied in this system. Neothramycin and tomaymycin cause additional blocks at some GGA-5' and TGA-5' sequences. Parallel MPE-Fe(II) footprinting studies have revealed PBD binding sites on both the transcribing and nontranscribing strands, although all transcription blocks determined from the IVTF assays are due to drug bound on the transcribing DNA template strand.
Subject(s)
Bacteriophage T7/enzymology , Benzodiazepinones/pharmacology , DNA-Directed RNA Polymerases/antagonists & inhibitors , Transcription, Genetic/drug effects , Anthramycin/pharmacology , Bacteriophage T7/drug effects , Bacteriophage T7/genetics , Base Sequence , DNA Footprinting , DNA-Directed RNA Polymerases/genetics , Edetic Acid/analogs & derivatives , Iron Chelating Agents , Molecular Sequence DataABSTRACT
Ecteinascidin 743 is one of several related marine alkaloids isolated from the Caribbean tunicate Ecteinascidia turbinata. It is remarkably active and potent in a variety of in vitro and in vivo systems and has been selected for development as an anticancer agent. The present study investigates the interactions of ecteinascidin 743 with DNA. Ecteinascidin 743 retarded the electrophoretic migration of both supercoiled and relaxed simian virus 40 DNA even in the presence of sodium dodecyl sulfate and after ethanol precipitation, consistent with covalent DNA modifications. Similar results were obtained in a DNA oligonucleotide derived from ribosomal DNA. However, DNA denaturation reversed the DNA modifications. The homopolymeric oligonucleotide dG/dC was modified while neither the dI/dC nor the dA/dT oligonucleotides were, consistent with covalent attachment of ecteinascidin 743 to the exocyclic amino group at position 2 of guanine. Ecteinascidin 743 was then compared to another known DNA minor groove alkylating agent, anthramycin, which has also been shown to alkylate guanine N2. Footprinting analyses with DNase I and 1,10-phenanthroline-copper and exonuclease III digestions showed that ecteinascidin 743 covers three to five bases of DNA and exhibits a different sequence specificity than anthramycin in the Escherichia coli tyrosine tRNA promoter (tyrT DNA). The binding of ecteinascidin to DNA was abolished when guanines were substituted with inosines in this promoter. A band shift assay was designed to evaluate the influence of the bases flanking a centrally located guanine in an oligonucleotide containing inosines in place of guanines elsewhere. Ecteinascidin 743 and anthramycin showed similarities as well as differences in sequence selectivity. Ecteinascidin 743-guanine adducts appeared to require at least one flanking guanine and were strongest when the flanking guanine was 3' to the targeted guanine. These data indicate that ecteinascidin 743 is a novel DNA minor groove, guanine-specific alkylating agent.
Subject(s)
Antineoplastic Agents, Alkylating/pharmacology , DNA/chemistry , DNA/drug effects , Dioxoles/pharmacology , Isoquinolines/pharmacology , Alkylation , Animals , Anthramycin/chemistry , Anthramycin/pharmacology , Antineoplastic Agents, Alkylating/chemistry , Base Sequence , Binding Sites , DNA/genetics , DNA Footprinting , Dioxoles/chemistry , Guanine/chemistry , Isoquinolines/chemistry , Molecular Sequence Data , Molecular Structure , Tetrahydroisoquinolines , Trabectedin , UrochordataSubject(s)
Anthramycin/pharmacology , Antibiotics, Antineoplastic/pharmacology , Cross-Linking Reagents/pharmacology , DNA/drug effects , DNA/metabolism , Leucomycins/pharmacology , Anthramycin/chemistry , Anthramycin/toxicity , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/toxicity , Base Sequence , Benzodiazepines/chemistry , Benzodiazepines/pharmacology , Benzodiazepines/toxicity , Cross-Linking Reagents/chemistry , Cross-Linking Reagents/toxicity , DNA Adducts/biosynthesis , Duocarmycins , Humans , Indoles/chemistry , Indoles/pharmacology , Indoles/toxicity , Leucomycins/chemistry , Leucomycins/toxicity , Molecular Sequence Data , Nucleic Acid ConformationABSTRACT
The repair of anthramycin-DNA adducts by the UVR proteins in Escherichia coli follows two pathways: the adducts may be incised by the combined actions of UVRA, UVRB, and UVRC, or alternatively, the anthramycin may be removed by UVRA and UVRB in the absence of UVRC and with no DNA strand incision. To assess the competition between these two competing pathways, the rate of UVRABC-mediated excision repair of anthramycin-N2-guanine DNA adducts and the rate of UVRAB-mediated removal of the adduct were measured with single end-labeled DNAs under identical reaction conditions. UVR protein concentrations of 15 nM UVRA, 100 nM UVRB, and 10 nM UVRC protein were chosen to mimic in vivo concentrations. With these UVR protein concentrations and anthramycin-DNA concentrations of 1-2 nM the incision reaction and the release reactions are described by first-order kinetics. The rate of the UVRABC reaction, measured as the increase in incised fragments, was six to seven times faster than the rate of the UVRAB reaction, measured as the decrease in incised fragments. The UVRABC incision rate on anthramycin-modified linear DNA was four to five times the incision rate measured on the same DNA irradiated with ultraviolet light. We also investigated the role of the ATPase function of UVRB in UVRAB-mediated anthramycin removal. We found that a UVRB analogue with alanine at arginine 51, which retains near wild type ATPase activity, supported removal of anthramycin in the presence of UVRA, whereas a UVRB analogue with alanine at lysine 45, which abolishes the ATPase activity, did not. UVRB*, a specific proteolytic cleavage product of UVRB which retains the ATPase activity, did support removal of anthramycin in the presence of UVRA.
Subject(s)
Anthramycin/metabolism , Anthramycin/pharmacology , DNA Adducts , DNA Repair , DNA, Bacterial/genetics , DNA/metabolism , Endodeoxyribonucleases/metabolism , Escherichia coli Proteins , Escherichia coli/genetics , Guanine , DNA, Bacterial/drug effects , DNA, Bacterial/radiation effects , Drug Combinations , Endodeoxyribonucleases/genetics , Escherichia coli/enzymology , Escherichia coli/radiation effects , Genes, Bacterial , Kinetics , Plasmids , Time FactorsABSTRACT
Various kinds of DNA damage block the 3' to 5' exonuclease action of both E. coli exonuclease III and T4 DNA polymerase. This study shows that a variety of DNA damage likewise inhibits DNA digestion by lambda exonuclease, a 5' to 3' exonuclease. The processive degradation of DNA by the enzyme is blocked if the substrate DNA is treated with ultraviolet irradiation, anthramycin, distamycin, or benzo[a]-pyrene diol epoxide. Furthermore, as with the 3' to 5' exonucleases, the enzyme stops at discrete sites which are different for different DNA damaging agents. On the other hand, digestion of treated DNA by lambda exonuclease is only transiently inhibited at guanine residues alkylated with the acridine mustard ICR-170. The enzyme does not bypass benzo[a]-pyrene diol epoxide or anthramycin lesions even after extensive incubation. While both benzo[a]-pyrene diol epoxide and ICR-170 alkylate the guanine N-7 position, only benzo[a]-pyrene diol epoxide also reacts with the guanine N-2 position in the minor groove of DNA. Anthramycin and distamycin bind exclusively to sites in the minor groove of DNA. Thus lambda exonuclease may be particularly sensitive to obstructions in the minor groove of DNA; alternatively, the enzyme may be blocked by some local helix distortion caused by these adducts, but not by alkylation at guanine N-7 sites.
Subject(s)
Aminoacridines , DNA Damage , DNA/metabolism , Exodeoxyribonucleases/metabolism , Anthramycin/pharmacology , Base Sequence , Benzopyrenes/pharmacology , Cisplatin/pharmacology , DNA/drug effects , DNA/radiation effects , Distamycins/pharmacology , Molecular Sequence Data , Nitrogen Mustard Compounds/pharmacology , Ultraviolet Rays , Viral ProteinsABSTRACT
A mutant murine cell line has previously been reported to be resistant to the AT-specific DNA minor groove ligand 2',5'-bi-1H-benzimidazole, 2',(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl), trichloride (Ho33342), due to an enhanced capacity to remove ligand molecules from cellular DNA via a pathway which can be blocked by DNA topoisomerase poisons. We have studied the relationship between ligand resistance and DNA topoisomerase II activity. The cross-sensitivity patterns of the mutant were examined for covalently (anthramycin) and non-covalently (distamycin A) binding minor groove ligands, and DNA intercalating [adriamycin, mitoxantrone and 4'-(9-acridinylamino)methanesulphon-m-anisidide (mAMSA)] and non-intercalating (VP16-213) topoisomerase II poisons. The mutant was cross-resistant to distamycin A alone. The mutant showed no abnormality in: (i) the in vitro decatenation activity of topoisomerase II, (ii) VP16-213 or mAMSA induced protein-DNA cross-linking activities in nuclear extracts, (iii) 'cleavable complex' generation (or DNA strand scisson) in intact cells exposed to topoisomerase poisons. Ho33342 and the topoisomerase II inhibitor novobiocin were found to disrupt both the in vitro binding of nuclear extracted proteins, from mutant and parental cells, to plasmid DNA and the formation of drug-induced cleavable complexes in vitro. Unexpectedly, Ho33342 induced significant levels of DNA-protein crosslinking in both parental and mutant cells. We conclude that: (i) resistance of the mutant is limited to non-covalently binding minor groove ligands, (ii) Ho33342 can block the trapping of DNA topoisomerase II by enzyme poisons in vitro, (iii) Ho33342 can induce a novel form of DNA-protein cross-link in intact cells, and (iv) the resistance of the mutant is not dependent upon some abnormality in topoisomerase II function.
Subject(s)
Anthramycin/pharmacology , Benzimidazoles/pharmacology , Benzodiazepinones/pharmacology , DNA Damage , DNA Topoisomerases, Type II/metabolism , DNA/drug effects , Distamycins/pharmacology , Pyrroles/pharmacology , Amsacrine/analogs & derivatives , Amsacrine/pharmacology , Animals , Cell Line , Cell Survival , DNA/metabolism , Doxorubicin/pharmacology , Drug Resistance/genetics , Intercalating Agents/pharmacology , Ligands , Mice , Mitoxantrone/pharmacology , Mutation , Nuclear Proteins/metabolismABSTRACT
Anthramycin (ATM) which is a product of some streptomyces micro-organisms was shown to antagonize the central effects of cholecystokinin (CCK) such as antinociception and satiety and to displace CCK bound to the slices from the brains of mice. Sulfated octapeptide CCK (CCK8) was administered intracisternally to mice at doses of 1 microgram/mouse for inducing antinociception and 200 ng/mouse for satiety. ATM was administered intraperitoneally to mice at doses such as 0.3 and 0.5 mg/kg. CCK8-induced antinociception and satiety were significantly reversed by ATM in those doses. [125I]CCK8 binding to the brain slices was observed autoradiographically. The autoradiograms from the slices were converted to false color images by using a microcomputer. The radioactivity in the autoradiograms was expressed by color spectra in the false color images. Comparison of the binding of [125I]CCK8 to the brain slices in the presence and the absence of ATM revealed that ATM (10(-6) M) clearly displaced the CCK8 binding in the various regions, especially in the cortex, of the brain. These findings suggest that ATM acts as an potent antagonist of CCK in the central nervous system in mice.
Subject(s)
Anthramycin/pharmacology , Benzodiazepines/pharmacology , Benzodiazepinones/pharmacology , Cholecystokinin/physiology , Feeding Behavior/drug effects , Pain/metabolism , Sensory Thresholds/drug effects , Animals , Anthramycin/metabolism , Benzodiazepines/metabolism , Cholecystokinin/metabolism , MiceABSTRACT
A new antitumor antibiotic porothramycin was produced by a new strain of Streptomyces albus. The antibiotic was isolated in two active forms, the natural free hydroxyl form (porothramycin A) or the crystalline methyl ether form (porothramycin B) depending upon the isolation process used. Structural studies established that porothramycin is a new member of the pyrrolo[1,4]benzodiazepine group antibiotics having only one substituent on the benzene ring. The antibiotic exhibited antimicrobial activity against Gram-positive bacteria and anaerobes and significantly prolonged the survival times of mice implanted with experimental tumors.
Subject(s)
Anthramycin/isolation & purification , Antibiotics, Antineoplastic/isolation & purification , Benzodiazepinones/isolation & purification , Animals , Anthramycin/analogs & derivatives , Anthramycin/pharmacology , Antibiotics, Antineoplastic/pharmacology , Chemical Phenomena , Chemistry , Gram-Positive Bacteria/drug effects , Hydrolysis , Leukemia L1210/drug therapy , Leukemia P388/drug therapy , Magnetic Resonance Spectroscopy , Melanoma, Experimental/drug therapy , Mice , Microbial Sensitivity Tests , Streptomyces/metabolismABSTRACT
Effects of diazepam (DZP), a synthetic benzodiazepine drug, and anthramycin (ATM), a benzodiazepine antitumor antibiotic produced by a certain species of streptomyces, on the uptake of 2-deoxy-D-[14C]-glucose (2-DG) in mouse brain neurons with or without cholecystokinin were examined. 2-DG uptake in neurons was evaluated by using an autoradiographic technique. The sulfated octapeptide CCK (CCK8) was injected intracisternally; DZP and ATM, intraperitoneally; and 2-DG, intravenously to mice. Autoradiograms prepared from the slices of the brain were converted to false color images. CCK8 (1 microgram/mouse) markedly stimulated the 2-DG uptake in neurons in the various regions of the brain, but the stimulative effects of CCK8 was almost completely suppressed after an intraperitoneal administration of 1.0 mg/kg of DZP or 0.5 mg/kg of ATM. Since it has been previously shown that these doses of DZP and ATM almost completely reversed the antinociception produced by 1 microgram/mouse of CCK8, the present results on the 2-DG uptake in the mouse brain are considered to further support the antagonism between CCK8 and DZP or ATM in the central nervous system.
Subject(s)
Anthramycin/pharmacology , Benzodiazepinones/pharmacology , Brain/drug effects , Cholecystokinin/antagonists & inhibitors , Diazepam/pharmacology , Animals , Autoradiography , Biological Transport, Active/drug effects , Brain/metabolism , Deoxyglucose/pharmacokinetics , Male , MiceABSTRACT
Antibiotics exhibiting immunomodulatory activities were found among antitumor antibiotics. These antibiotics had antileukemic activity. Neothramycin and mazethramycin, which are classified as anthramycin-group antibiotics, activated macrophages so that they became antitumor effector cells. Aclacinomycin and oxanosine inhibited generation of suppressor cells in tumor-bearing mice and oxanosine enhanced antitumor effector cells. Therapy using spergualin produced specific antitumor immunity in cured mice. The immunomodulatory activities of auromomycin and bactobolin were also reported.