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1.
Viruses ; 13(2)2021 01 21.
Article in English | MEDLINE | ID: mdl-33494175

ABSTRACT

Zika virus (ZIKV) RNA has been found to remain in human semen for up to one year after infection, but the presence of Flavivirus antigens in the different compartments of semen has been largely unexplored. Following the introduction of ZIKV in Nicaragua (2016), a prospective study of patients with clinical symptoms consistent with ZIKV was conducted in León to investigate virus shedding in different fluids. ZIKV infection was confirmed in 16 male subjects (≥18 years of age) by RT-qPCR in either blood, saliva or urine. Of these, three provided semen samples at 7, 14, 21, 28, 60 and 180 days postsymptom onset (DPSO) for Flavivirus antigens and RNA studies. These cases were compared with 19 asymptomatic controls. Flavivirus antigens were examined by immunofluorescence (IF) using the 4G2 Mabs, and confocal microscopy was used to explore fluorescence patterns. The three (100%) symptomatic subjects and 3 (16%) of the 19 asymptomatic subjects had Flavivirus antigens and viral RNA in the spermatozoa fraction. The percentage of IF Flavivirus-positive spermatozoa cells ranged from 1.9% to 25% in specimens from symptomatic subjects, as compared with 0.8% to 3.8% in specimens from asymptomatic controls. A marked IF-pattern in the cytoplasmic droplets and tail of the spermatozoa was observed. The sperm concentrations (45 × 106/mL vs. 63.5 × 106/mL, p = 0.041) and the total motility percentage (54% vs. 75%, p = 0.009) were significantly lower in specimens from ZIKV-positive than in those of ZIKV-negative. In conclusion, this study demonstrated the presence of Flavivirus antigens and RNA within a time frame of 28 DPSO in sperm cells of symptomatic and asymptomatic subjects during the ZIKV epidemic. These findings have implications for public health, in terms of nonarthropod-born, silent transmission facilitated by sperm cells and potential transmission from asymptomatic males to pregnant women, with consequences to the fetus.


Subject(s)
Antigens, Viral/analysis , Flavivirus/isolation & purification , RNA, Viral/analysis , Spermatozoa/virology , Zika Virus Infection/virology , Zika Virus/isolation & purification , Adult , Antigens, Viral/blood , Antigens, Viral/urine , Flavivirus/genetics , Fluorescent Antibody Technique , Humans , Male , RNA, Viral/blood , RNA, Viral/urine , Real-Time Polymerase Chain Reaction , Saliva/virology , Semen/virology , Spermatozoa/chemistry , Virus Shedding , Young Adult , Zika Virus/genetics
2.
J Infect ; 78(3): 232-240, 2019 03.
Article in English | MEDLINE | ID: mdl-30659856

ABSTRACT

OBJECTIVES: Hepatitis E virus genotype 3 (HEV3) is responsible for acute and chronic liver disease in solid organ transplant (SOT) recipients. HEV was recently found in the urine of some acutely and chronically genotype 4-infected patients. METHODS: We examined the urinary excretion of HEV3 by 24 consecutive SOT recipients at the acute phase of HEV hepatitis and characterized the excreted virus. RESULTS: Urinary HEV RNA was detected in 12 (50%) of the 24 transplanted patients diagnosed with HEV hepatitis. Urinary HEV antigen (Ag) was detected in all but one of the patients (96%). The density of RNA-containing HEV particles in urine was low (1.11-1.12 g/cm3), corresponding to lipid-associated virions. The urinary HEV RNA/Ag detected was not associated with impaired kidney function or de novo proteinuria. Finally, there was more HEV Ag in the serum at the acute phase of HEV infection in SOT recipients whose infection became chronic. CONCLUSIONS: HEV3 excreted via the urine of SOT recipients at the acute phase of HEV hepatitis has a lipid envelope. Renal function was not impaired. While urinary HEV Ag was a sensitive indicator of HEV infection, only acute phase serum HEV Ag indicated the development of a chronic infection.


Subject(s)
Hepatitis E/diagnosis , Immunocompromised Host , Viral Proteins/blood , Viral Proteins/urine , Acute Disease , Adult , Antigens, Viral/blood , Antigens, Viral/urine , Female , Genotype , Hepatitis E/blood , Hepatitis E/urine , Hepatitis E virus/genetics , Hepatitis E virus/isolation & purification , Humans , Male , Middle Aged , RNA, Viral/genetics , RNA, Viral/urine , Transplant Recipients
3.
Clin Lab ; 61(3-4): 299-306, 2015.
Article in English | MEDLINE | ID: mdl-25974996

ABSTRACT

BACKGROUND: Urine is an important source for the detection of infections caused by CMV in stem cell transplant patients. Currently, there is no agreement about the type of urine specimen. In order to investigate which is the better specimen type for quantitative detection of CMV, we compared the results from urine supernatant and sediment from the same patients. METHODS: Seventy urine specimens were collected from patients with hematological disorders or solid tumors. After performing shell vial culture, residual urine specimens were centrifuged. Then, 10 mL of each urine supernatant and sediment were taken and immediately frozen at -70 degrees C. Afterwards, archived urine specimens were thawed at room temperature and CMV-quantitative PCR was performed on both the supernatant and sediment fraction of urine. The results from each patient were reviewed for CMV antigenemia, blood shell vial culture, CMV-IgM or IgG, and clinical symptoms. RESULTS: CMV-qPCR results for the urine sediment fraction revealed a significant difference (p = 0.012) between the active CMV infection group and the latent CMV infection group. In addition, receiver operating characteristic curves for active CMV infection revealed that CMV-qPCR using urine sediment produced more accurate results than urine supernatant. CONCLUSIONS: These findings suggest that the sediment fraction of urine is a more suitable specimen in CMV-qPCR testing.


Subject(s)
Cytomegalovirus Infections/urine , Cytomegalovirus/isolation & purification , Stem Cell Transplantation/adverse effects , Urinalysis/methods , Viremia/urine , Adolescent , Adult , Antibodies, Viral/urine , Antigens, Viral/urine , Child , Child, Preschool , Cytomegalovirus/genetics , Female , Hematologic Neoplasms/therapy , Hematologic Neoplasms/urine , Humans , Immunoglobulin G/urine , Immunoglobulin M/urine , Infant , Male , Middle Aged , Neoplasms/therapy , Neoplasms/urine , Real-Time Polymerase Chain Reaction , Specimen Handling , Young Adult
4.
J Int AIDS Soc ; 17: 19040, 2014.
Article in English | MEDLINE | ID: mdl-25109284

ABSTRACT

BACKGROUND: Detection of subclinical cryptococcal disease using cryptococcal antigen screening among HIV-positive individuals presents a potential opportunity for prevention of both clinical disease and death if patients with detectable cryptococcal antigen are identified and treated pre-emptively. Recently developed point-of-care cryptococcal antigen tests may be useful for screening, particularly in resource-limiting settings, but few studies have assessed their utility. METHODOLOGY: The objectives of this study were to determine the prevalence and factors associated with cryptococcal antigenemia in HIV-positive patients with CD4(+) T-cell counts ≤200 cells/µL who were initiating ART, and also to evaluate the utility of the point-of-care urine lateral flow assay (LFA) cryptococcal antigen test using two different diluents, compared to gold standard serum antigen testing, as a screening tool. Urine and serum of outpatients initiating antiretroviral therapy at two hospitals in Mwanza were tested for cryptococcal antigen, and demographic and clinical characteristics were obtained using structured questionnaires and patients' files. Patients with asymptomatic cryptococcal antigenemia received oral fluconazole in accordance with World Health Organization recommendations. RESULTS: Among 140 patients screened, 10 (7.1%) had asymptomatic cryptococcal antigenemia with a positive serum cryptococcal antigen. Four of these ten patients had CD4 counts between 100 and 200 cells/µL. The prevalence of cryptococcal antigen detected in urine using a standard (older) and a test (newer) diluent were 44 (31.4%) and 19 (13.6%), with Kappa coefficients compared to serum of 0.28 and 0.51 (p<0.001 for both). Compared to the new LFA diluent for urine cryptococcal antigen, the standard diluent had higher sensitivity (100% versus 80%) but lower specificity (74% versus 92%) using serum cryptococcal antigen as a gold standard. CONCLUSIONS: Our findings suggest that HIV-positive outpatients with CD4 counts <200 cells/µL, rather than 100, should be screened for asymptomatic cryptococcal antigenemia given its association with mortality if untreated. Agreement of the urine LFA with the serum LFA was not sufficient to recommend routine screening with urine LFA.


Subject(s)
Antigens, Viral/blood , Antigens, Viral/urine , Chromatography, Affinity/methods , Cryptococcosis/diagnosis , Cryptococcus/immunology , Diagnostic Tests, Routine/methods , HIV Infections/complications , Adult , Asymptomatic Infections/epidemiology , Cross-Sectional Studies , Cryptococcosis/epidemiology , Cryptococcus/isolation & purification , Female , Humans , Male , Outpatients , Point-of-Care Systems , Prevalence , Risk Factors , Serum/microbiology , Tanzania/epidemiology , Urine/microbiology
5.
Clin Infect Dis ; 54 Suppl 2: S180-9, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22403234

ABSTRACT

We conducted a prospective pilot study over a 1-year period in New Caledonia in preparation for the Pneumonia Research for Child Health (PERCH) project. The pathogens associated with hospitalized lower respiratory infections in children were identified through the use of culture of induced sputum and blood, urinary antigen detection, polymerase chain reaction (PCR) on respiratory specimens, and serology on paired sera. Respiratory viruses were detected on respiratory specimens by immunofluorescence and PCR, and by serology on paired sera. Pathogens were detected in 87.9% of the 108 hospitalized cases. Viruses represented 81.6% of the 152 pathogens detected. Respiratory syncytial virus and rhinovirus were the most frequent, accounting for 32.2% and 24.3% of the pathogens identified, respectively. Only 26.3% of 99 induced sputum specimens collected were determined to be of good quality, which may be a consequence of the collection method used.


Subject(s)
Child, Hospitalized/statistics & numerical data , Pneumonia/etiology , Respiratory Syncytial Viruses/isolation & purification , Respiratory Tract Infections/epidemiology , Adolescent , Antigens, Viral/urine , Bacteria/isolation & purification , Bacteria/pathogenicity , Bacterial Infections/blood , Bacterial Infections/epidemiology , Bacterial Infections/virology , Case-Control Studies , Child , Child, Preschool , Fluorescent Antibody Technique , Humans , Infant , New Caledonia/epidemiology , Picornaviridae Infections/blood , Picornaviridae Infections/epidemiology , Picornaviridae Infections/virology , Pilot Projects , Prospective Studies , Respiratory Syncytial Virus Infections/blood , Respiratory Syncytial Virus Infections/epidemiology , Respiratory Syncytial Virus Infections/virology , Respiratory Syncytial Viruses/pathogenicity , Respiratory Tract Infections/microbiology , Respiratory Tract Infections/virology , Rhinovirus/isolation & purification , Rhinovirus/pathogenicity , Serologic Tests , Specimen Handling/methods , Sputum/microbiology , Sputum/virology
7.
J Virol ; 83(10): 5046-55, 2009 May.
Article in English | MEDLINE | ID: mdl-19279096

ABSTRACT

Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.


Subject(s)
Antigens, Viral/urine , Hantavirus Pulmonary Syndrome/urine , Orthohantavirus/immunology , beta 2-Glycoprotein I/immunology , Animals , Antibodies, Monoclonal/immunology , Antibodies, Viral/blood , Antibodies, Viral/immunology , Antigens, Viral/blood , Antigens, Viral/immunology , Chlorocebus aethiops , Enzyme-Linked Immunosorbent Assay , Hantavirus Pulmonary Syndrome/blood , Hantavirus Pulmonary Syndrome/immunology , Humans , Immunoglobulin G/blood , Immunoglobulin G/immunology , Microspheres , RNA, Viral/analysis , Vero Cells
8.
Acta Cytol ; 52(2): 191-5, 2008.
Article in English | MEDLINE | ID: mdl-18499992

ABSTRACT

OBJECTIVE: To compare the diagnostic value of cytology and immunohistochemistry staining (IHS) of urine samples for polyomavirus reactivation diagnosis. STUDY DESIGN: Sixty-eight urine samples collected from 18 immunosuppressed patients were analyzed by Papanicolaou and IHS with a JC/BK virus-specific monoclonal antibody. RESULTS: Overall, polyomavirus BK (BKV) was positive in 11 of 18 patients (61.1%) (3 of whom developed hemorrhagic cystitis) and in 23 of 68 urine samples (28%). Of 23 samples, 4 (17%) were positive by 1 of the 2 techniques, only. Of 23 samples, 19 (83%) were positive by both methods. In matching urine samples from the same patient, the number of BKV-infected positive cells detected by IHS in urine slides was higher than those detected by Papanicolaou staining (71.3%). CONCLUSION: The main advantage of LHS is that it allowed confirmation of BKV infection diagnosis in urine samples. IHS detected more BKV-infected cells in samples with few positive urothelial cells, which would have gone undetected if only Papanicolaou staining had been used as the BKV screening method. Urine samples testing for BKV by both techniques will improve diagnosis in asymptomatic patients, allowing early therapeutic intervention and a better clinical outcome.


Subject(s)
Antigens, Viral/urine , BK Virus/immunology , Cord Blood Stem Cell Transplantation , Immunohistochemistry , Immunosuppressive Agents/adverse effects , JC Virus/immunology , Kidney Transplantation , Polyomavirus Infections/virology , Adult , Antibodies, Monoclonal , BK Virus/pathogenicity , False Positive Reactions , Female , Humans , JC Virus/pathogenicity , Male , Middle Aged , Polyomavirus Infections/urine , Predictive Value of Tests , Reproducibility of Results , Staining and Labeling/methods , Urine/cytology , Urine/virology , Urothelium/pathology , Urothelium/virology
9.
J Infect Dis ; 196 Suppl 2: S193-8, 2007 Nov 15.
Article in English | MEDLINE | ID: mdl-17940949

ABSTRACT

Between the months of April and June 2004, an Ebola hemorrhagic fever (EHF) outbreak was reported in Yambio county, southern Sudan. Blood samples were collected from a total of 36 patients with suspected EHF and were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G and M antibodies, antigen ELISA, and reverse-transcription polymerase chain reaction (PCR) of a segment of the Ebolavirus (EBOV) polymerase gene. A total of 13 patients were confirmed to be infected with EBOV. In addition, 4 fatal cases were classified as probable cases, because no samples were collected. Another 12 patients were confirmed to have acute measles infection during the same period that EBOV was circulating. Genetic analysis of PCR-positive samples indicated that the virus was similar to but distinct from Sudan EBOV Maleo 1979. In response, case management, social mobilization, and follow-up of contacts were set up as means of surveillance. The outbreak was declared to be over on 7 August 2004.


Subject(s)
Ebolavirus/isolation & purification , Hemorrhagic Fever, Ebola/diagnosis , Hemorrhagic Fever, Ebola/epidemiology , Adolescent , Adult , Antigens, Viral/blood , Antigens, Viral/urine , Child , Disease Outbreaks , Ebolavirus/immunology , Enzyme-Linked Immunosorbent Assay , Female , Filtration , Hemorrhagic Fever, Ebola/blood , Hemorrhagic Fever, Ebola/urine , Humans , Immunoassay , Infant , Male , Sensitivity and Specificity , Sudan/epidemiology
10.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 38(6): 969-72, 2007 Nov.
Article in Chinese | MEDLINE | ID: mdl-18095598

ABSTRACT

OBJECTIVE: To explore the relationship between respiratory virus infection and the episode of steroid responsive simple nephrotic syndrome (SRSNS). METHODS: Thirty eight children with SRSNS were recruited (28 in the active stage, 10 in the remission stage). Sixty four children (18 with nephritic nephrosis, 16 with bronchiolitis, 15 with secondary glomerular diseases and 15 without diagnosed diseases) served as controls. Reverse transcriptase-PCR (RT-PCR) and alkaline phosphoesterase-anti alkaline phosphoesterase enzyme-linked assay (APAAP) were employed to detect the viral genes and antigens in the urines respectively. The viral antigens in the renal tissues of two children with active SRSNS were also examined by APAAP. RESULTS: The viruses were more often detected in the urines of children with active SRSNS than those with remission SRSNS and the controls. The respiratory syncytial virus (RSV) was the most common virus detected in the urines of children with active SRSNS. The appearance of the viruses gene and antigens was not influenced by the use of steroid. The same antigens were found in the renal tissues of the two children with active SRSNS. CONCLUSION: Respiratory tract viruses may play an important role of triggering the SRSNS.


Subject(s)
Antigens, Viral/urine , Kidney/virology , Nephrotic Syndrome/virology , Respiratory Syncytial Virus Infections/virology , Respiratory Syncytial Virus, Human/isolation & purification , Adolescent , Child , Child, Preschool , Humans , Kidney/pathology
11.
Arch Dis Child ; 92(12): 1109-12, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17652319

ABSTRACT

INTRODUCTION: The aim of this study was to assess the clinical significance and prognosis of a prolonged isolated elevation of serum aminotransferases without cholestasis (>3 months) in infants and young children, investigated for a variety of conditions, and to determine a protocol for their follow-up and investigation. METHODS: A combined prospective-retrospective analysis of apparently healthy babies and young children with isolated elevation of serum aminotransferases of at least 1.5 times above the norm for age which persisted for at least 3 months and whose creatine phosphokinase (CK), gamma glutamyltransferase (GGT), alkaline phosphatase and bilirubin levels remained normal throughout the study duration. The children underwent the following investigations: abdominal ultrasound and infectious, metabolic and/or immunological investigation depending on the duration of the abnormality. RESULTS: Six children were eliminated following the finding of positive cytomegalovirus (CMV) antigen in the urine. 72 children were investigated (47 males and 25 females). The duration of serum aminotransferases elevation was 3-36 months (average 12.4, median 11.5 months). The initial, maximal and final alanine aminotransferase (ALT) values were 85.5, 140.5 and 39.8 IU/l, respectively. Of seven children who had liver biopsies performed, three (42.8%) were suspected of having a glycogen storage disease which was not confirmed enzymatically. Four biopsies revealed non-specific histological changes. CONCLUSIONS: Isolated elevation of serum aminotransferases in healthy looking young children is mostly a benign condition that usually resolves within a year. If no pathology is found during routine investigation, these children can be followed conservatively. Liver biopsy does not contribute much to the diagnosis and is probably unnecessary.


Subject(s)
Transaminases/blood , Alanine Transaminase/blood , Antigens, Viral/urine , Aspartate Aminotransferases/blood , Biomarkers/blood , Biopsy , Breast Feeding , Child, Preschool , Cytomegalovirus/isolation & purification , Female , Glycogen Storage Disease/diagnosis , Humans , Infant , Infant, Newborn , Liver/pathology , Male , Prognosis , Prospective Studies , Retrospective Studies , Unnecessary Procedures
12.
Vopr Virusol ; 50(1): 9-14, 2005.
Article in Russian | MEDLINE | ID: mdl-15747864

ABSTRACT

31 prematures with signs of the cytomegalovirus infection (CMV) were examined. The blood and urine samples were tested for direct viral markers, i.e. for infectious CMV by the rapid culture method (RCM) and for viral DNA by quantitative PCR. Besides, the parameters of the specific immune response were studied in the babies. CMV was detected by RCM and/or PCR in 25 of the 31 examined babies during their 1st life week. The highest content of CMV within the investigated samples, i.e. 100 antigen-containing cells per 2.5 x 10(5) culture cells and above 2000 copies/ml of viral DNA was detected in 8 (32%) children. The quantity of viral DNA did not exceed 1000 copies/ml and one to three of stained cells was detected by PCR in 13 (42%) children. A study of anti-CMV in sera revealed high-titer of AT IgG in all 30 children. High avidity of anti-CMV-IgG was demonstrated to correlate with a low viral load and a low CMV infection activity in the newborns. According to the results, at least 3 laboratory diagnosis tools should be used in the diagnosis, they are PCR, RCM and determination of the anti-CMV avidity.


Subject(s)
Cytomegalovirus Infections/diagnosis , Cytomegalovirus/isolation & purification , Infant, Premature , Antibodies, Viral/blood , Antibody Affinity , Antigens, Viral/blood , Antigens, Viral/urine , Cells, Cultured , Cytomegalovirus/genetics , Cytomegalovirus/immunology , Cytomegalovirus Infections/blood , Cytomegalovirus Infections/urine , DNA, Viral/blood , DNA, Viral/urine , Humans , Immunoglobulin G/blood , Infant, Newborn , Infant, Premature/blood , Infant, Premature/urine , Polymerase Chain Reaction , Virus Cultivation
15.
J Neurovirol ; 6 Suppl 2: S101-8, 2000 May.
Article in English | MEDLINE | ID: mdl-10871796

ABSTRACT

Two features of the biology of JC virus make it a particularly suitable candidate for an agent in MS-like disease: its neurotropic capability targeting glial cells as evidenced in progressive multifocal leukoencephalopathy lesions, and its capacity for latency and persistence as illustrated by its behaviour in the kidney. JC virus is chronically or intermittently excreted in the urine by some 40% of the population. The existence of JC virus in multiple coding-region genotypes provides a unique approach to the study of JC virus-induced neurological disease. We have previously shown that a genotype originating in Asia but also present in Europe and the US, called Type 2B, is more frequently found in PML brain than expected based on its prevalence in urine samples from a control population. In contrast, we find that the excretion of JCV in MS patients is similar in both genotype and frequency to that of control individuals, and appears to be regulated by factors unrelated to those that control CNS disease activity.


Subject(s)
CCAAT-Enhancer-Binding Proteins , JC Virus/genetics , Leukoencephalopathy, Progressive Multifocal/virology , Multiple Sclerosis, Chronic Progressive/virology , Multiple Sclerosis, Relapsing-Remitting/virology , Transcription Factors , Adjuvants, Immunologic/administration & dosage , Antigens, Viral/cerebrospinal fluid , Antigens, Viral/urine , Cohort Studies , DNA-Binding Proteins/genetics , Demyelinating Diseases/virology , Disease Progression , Female , Genes, Viral/genetics , Genotype , Humans , Interferon beta-1a , Interferon beta-1b , Interferon-beta/administration & dosage , JC Virus/isolation & purification , Leukoencephalopathy, Progressive Multifocal/drug therapy , Leukoencephalopathy, Progressive Multifocal/ethnology , Male , Multiple Sclerosis, Chronic Progressive/drug therapy , Multiple Sclerosis, Chronic Progressive/ethnology , Multiple Sclerosis, Relapsing-Remitting/drug therapy , Multiple Sclerosis, Relapsing-Remitting/ethnology , NFI Transcription Factors , Neuroglia/virology , Nuclear Proteins , Regulatory Sequences, Nucleic Acid , Risk Factors , Y-Box-Binding Protein 1
16.
Dtsch Med Wochenschr ; 125(15): 445-51, 2000 Apr 14.
Article in German | MEDLINE | ID: mdl-10800441

ABSTRACT

OBJECTIVE: Cytomegalovirus (CMV) infection is the most common viral infection in the early period after heart transplantation (HTX) and causes a significant morbidity and mortality. Although controversial, CMV is related to acute and chronic allograft rejection and to the development of graft vascular disease. It therefore plays an important role in the long-time outcome after solid organ transplantation. PATIENTS AND METHODS: 45 patients received a new heart between 1.1.97 and 31.12.1998. All of them were enrolled postoperatively in three-month antiviral prophylaxis (Cymevene). Only those patients were excluded from prophylaxis who were seronegative for CMV and received hearts from seronegative donors (n = 6). The pp65 antigenaemia assay and the murex hybrid capture CMV DNA assay on peripheral blood as well as the early antigen detection in the urine were used for CMV detection and also for monitoring. RESULTS: A total number of 580 assays were analysed (12.9 assays/patient). 561 tests (96.7%) were negative, 19 (3.3%) were positive. For CMV testing the pp65 antigenemia assay was used in 64.1%, the murex hybrid capture CMV DNA assay in 18.4% and the urine early antigen detection in 17.4%. Three patients (6.7%) developed viraemia during the first 3 postoperative months. Two patients (4.4%) suffered from CMV infection 8 and 9 months after heart transplantation and had to be treated with antiviral agents. Three patients (6.7%) died early after transplantation, but none had a CMV infection. CONCLUSION: Prevention of CMV disease was successful with three months of antiviral CMV prophylaxis after HTX. Asymptomatic viraemia during the prophylaxis period did not lead to tissue invasive disease. It is possible to carry out rapid CMV detection and CMV monitoring with the commercially available antigenaemia assays.


Subject(s)
Antiviral Agents/therapeutic use , Cytomegalovirus Infections/prevention & control , Ganciclovir/therapeutic use , Heart Transplantation , Postoperative Complications/prevention & control , Adolescent , Adult , Aged , Antigens, Viral/blood , Antigens, Viral/urine , Cytomegalovirus/genetics , Cytomegalovirus/immunology , Cytomegalovirus/isolation & purification , Cytomegalovirus Infections/complications , Cytomegalovirus Infections/diagnosis , DNA, Viral/analysis , Female , Graft Occlusion, Vascular/etiology , Graft Rejection/etiology , Humans , Male , Middle Aged , Retrospective Studies , Tissue Donors/statistics & numerical data , Viremia/prevention & control
18.
Rinsho Ketsueki ; 39(6): 460-2, 1998 Jun.
Article in Japanese | MEDLINE | ID: mdl-9695676

ABSTRACT

We report on a case of adenovirus hemorrhagi cystitis that developed in a 49-year-old woman during intensive chemotherapy for adult T-cell leukemia. Although rapid etiologic diagnosis is essential for the effective management of viral hemorrhagic cystitis, the isolation of adenovirus from urine is often too time-cousuming. We detected the adenovirus antigen in the patient's urine using an Adenoclone direct sandwich ELISA kit (Cambridge Bio Science), which is commonly employed for the diagnosis of adenovirus conjunctivitis. Treatment consisted of intravenous vidarabine and bladder irrigation, which resulted in prompt clinical improvement. The Adenoclone kit was useful for the rapid etiologic diagnosis of adenovirus hemmorrhagic cystitis.


Subject(s)
Adenoviridae Infections/diagnosis , Cystitis/diagnosis , Leukemia, T-Cell/complications , Antigens, Viral/urine , Enzyme-Linked Immunosorbent Assay , Female , Humans , Middle Aged , Reagent Kits, Diagnostic
19.
J Virol Methods ; 73(1): 41-52, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9705173

ABSTRACT

Rapid, sensitive and specific assays are required for the diagnosis of CMV infection following transplantation. We describe our experience in developing assays for detecting CMV in urine. Conventional preparation of probes cloned after amplification in E. coli led to contamination with E. coli nucleic acids; these hybridised to E. coli DNA present in urine and produced false positive results. Two CMV probes (Hind III and gL) hybridised to human DNA despite high stringency; these probes were thus unsuitable for detecting viral nucleic acids in clinical samples. A PCR derived probe from the immediate early gene of CMV detected dot-blotted CMV DNA specifically. Optimal preparation of urine for detection of CMV DNA was as follows; four freeze/thaw cycles and ultracentrifugation before in vitro proteinase K/SDS treatment, phenol:chloroform extraction, heat denaturation and direct application onto a nylon membrane. However, dot-blot hybridisation was a poor test for CMV in urine; it had low sensitivity and specificity compared with virus isolation and DEAFF. Single round PCR of a 293 bp region of CMV DNA was sensitive and specific to CMV targets. However, undiluted urine contained PCR inhibitors that could only be partly removed by using PEG precipitation. PCR of CMV DNA from urine was specific but was insensitive compared to conventional culture and DEAFF. A significant proportion of urine samples were toxic in conventional culture and DEAFF tests but, PCR of CMV DNA from urine is insensitive and despite its specificity is unlikely to be advantageous in clinical practice even when DEAFF or culture prove unreliable.


Subject(s)
Cytomegalovirus/isolation & purification , DNA, Viral/urine , Nucleic Acid Hybridization/methods , Polymerase Chain Reaction/methods , Antigens, Viral/urine , Cells, Cultured , Cytomegalovirus/genetics , DNA Probes , Escherichia coli/genetics , False Positive Reactions , Fluorescent Antibody Technique, Direct , Humans , Sensitivity and Specificity
20.
Rev Clin Esp ; 198(1): 3-6, 1998 Jan.
Article in Spanish | MEDLINE | ID: mdl-9534340

ABSTRACT

OBJECTIVE: To prospectively and comparatively study the usefulness of urine (viruria) and blood (antigenemia pp65 and culture) (viremia) for the diagnosis of cytomegalovirus (CMV) infection in renal transplant (RT) recipients. MATERIAL AND METHODS: All RT recipients at our hospital were studied from January 1995 to December 1996. After decontamination, urine specimens were inoculated into two MRC-5 cell line vials. Polymorphonuclear cells were extracted from peripheral blood by sedimentation in saline dextran and were used for antigenemia pp65 test and for culture in shell-vial. RESULTS: A total of 1,335 specimens from 43 patients were studied. CMV was recovered from 110 out of the 913 (12%) urine specimens and from 101 out of the 422 (23.9%) blood specimens (antigenemia and/or viremia). CMV detection was first obtained by a positive blood test in 23 patients (88.4%), whereas the urine specimen was the first positive test in only three (11.6%) patients (p = 0.0001). A positive result in blood preceded a positive result in urine by a mean of 10.3 days (range: 2-73 days). Antigenemia and viremia were simultaneously positive in 61.5% of patients. In three patients a positive antigenemia preceded viremia by 14 days. In seven patients (26.9%) only the shell-vial culture was positive. Culture preceded antigenemia by a mean of 7.6 days. In the 26 patients, the time elapsed until the first positive blood specimen for CMV was 37.3 days (range: 11-88 days). CONCLUSION: According to the results obtained we believe that blood (antigenemia pp65 and/or viremia) should be considered as the only really useful specimen for the diagnosis of infection/disease caused by CMV in RT recipients. The urine specimen lacks a diagnostic and clinical usefulness and therefore should not be used in these patients.


Subject(s)
Cytomegalovirus Infections/diagnosis , Cytomegalovirus/isolation & purification , Kidney Transplantation , Opportunistic Infections/diagnosis , Antigens, Viral/blood , Antigens, Viral/urine , Cytomegalovirus Infections/blood , Cytomegalovirus Infections/urine , Humans , Opportunistic Infections/blood , Opportunistic Infections/urine , Prospective Studies , Viremia
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