ABSTRACT
BACKGROUND: Apolipoprotein E (apoE) is closely involved in the pathogenesis of apoE-related diseases, such as Alzheimer's disease and cardiovascular disease. The redox modulation of cysteine-thiols in a protein is involved in various pathophysiological regulations; however, that of apoE has not been studied in detail. Herein, we devised an analytical method to determine the redox status of serum apoE and assessed its relation to serum cholesterol levels and apoE phenotype. METHODS: The present method was based on a band shift assay, using a photocleavable maleimide-conjugated polyethylene glycol. RESULTS: The basic characteristics of the present method were found to be satisfactory to determine the redox status of serum apoE quantitatively. Serum apoE was separated into its reduced-form (r-), non-reduced-form (nr-), apoE-AII complex, and homodimer using this method. R-apoE could be detected as a 40-kDa band, whereas nr-apoE remained as monomeric apoE. R-apoE displayed a preference for VLDL; however, the levels showed the correlation with HDL-cholesterol levels (p<0.005). Redox status of serum apoE was significantly different among apoE phenotypes. The quantitative ratios of nr-apoE to total apoE in serum from subjects with apoE4/E3 were higher than in serum from subjects with apoE3/E3 (p<0.0001) and apoE3/E2 (p<0.001). CONCLUSION: The redox status of serum apoE might be related to the synthesis of HDL. The information concerning the redox status of serum apoE provided by the present method may be a potent indicator to evaluate various apoE-related diseases.
Subject(s)
Apolipoproteins E/blood , Cholesterol, HDL/blood , Apolipoprotein A-II/blood , Apolipoprotein A-II/chemistry , Apolipoprotein A-II/isolation & purification , Apolipoprotein E2/blood , Apolipoprotein E2/chemistry , Apolipoprotein E2/isolation & purification , Apolipoprotein E3/blood , Apolipoprotein E3/chemistry , Apolipoprotein E3/isolation & purification , Apolipoprotein E4/blood , Apolipoprotein E4/chemistry , Apolipoprotein E4/isolation & purification , Apolipoproteins E/chemistry , Apolipoproteins E/isolation & purification , Cholesterol, HDL/chemistry , Cysteine/chemistry , Diamide/chemistry , Dimerization , Dithiothreitol/chemistry , Electrophoretic Mobility Shift Assay , HEK293 Cells , Humans , Indicators and Reagents/chemistry , Molecular Weight , Oxidation-Reduction , Photochemical Processes , Polyethylene Glycols/chemistry , Solubility , Sulfhydryl Reagents/chemistry , Ultraviolet RaysABSTRACT
Since the discovery of the association of apolipoprotein E (apoE) 4 with Alzheimer's disease 17 years ago, numerous in vitro experiments with the apoE isoforms (apoE2, apoE3, and apoE4) have been performed to try to understand the basis for this association. The majority of these studies used commercial sources for apoE, but some used recombinant protein. In either case, these studies were most often conducted without considering the ramifications of the structural and biophysical differences among the three isoforms or without adequate quality control of the preparations. Here, we present a protocol for producing recombinant apoE that we have used successfully in our laboratory for the last 20 years. We also review the considerations that are critical for obtaining reliable and interpretable results with the end product.
Subject(s)
Apolipoproteins E/isolation & purification , Apolipoproteins E/metabolism , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Alzheimer Disease/metabolism , Apolipoprotein E2/genetics , Apolipoprotein E2/isolation & purification , Apolipoprotein E2/metabolism , Apolipoprotein E3/genetics , Apolipoprotein E3/isolation & purification , Apolipoprotein E3/metabolism , Apolipoprotein E4/genetics , Apolipoprotein E4/isolation & purification , Apolipoprotein E4/metabolism , Apolipoproteins E/genetics , Bacteria/genetics , Bacteria/metabolism , Chromatography, Gel , Electrophoresis, Polyacrylamide Gel , Recombinant Proteins/geneticsABSTRACT
Apolipoprotein E (apoE) is an exchangeable apolipoprotein that plays an important role in lipid/lipoprotein metabolism and cardiovascular diseases. Recent evidence indicates that apoE is also critical in several other important biological processes, including Alzheimer's disease, cognitive function, immunoregulation, cell signaling, and infectious diseases. Although the X-ray crystal structure of the apoE N-terminal domain was solved in 1991, the structural study of full-length apoE is hindered by apoE's oligomerization property. Using protein-engineering techniques, we generated a monomeric, biologically active, full-length apoE. Cross-linking experiments indicate that this mutant is nearly 95-100% monomeric even at 20 mg/mL. CD spectroscopy and guanidine hydrochloride denaturation demonstrate that the structure and stability of the monomeric mutant are identical to wild-type apoE. Monomeric and wild-type apoE display similar lipid-binding activities in dimyristoylphosphatidylcholine clearance assays and formation of reconstituted high-density lipoproteins. Furthermore, the monomeric and wild-type apoE proteins display an identical LDL receptor binding activity. Availability of this monomeric, biologically active, full-length apoE allows us to collect high quality NMR data for structural determination. Our initial NMR data of lipid-free apoE demonstrates that the N-terminal domain in the full-length apoE adopts a nearly identical structure as the isolated N-terminal domain, whereas the C-terminal domain appears to become more structured than the isolated C-terminal domain fragment, suggesting a weak domain-domain interaction. This interaction is confirmed by NMR examination of a segmental labeled apoE, in which the N-terminal domain is deuterated and the C-terminal domain is double-labeled. NMR titration experiments further suggest that the hinge region (residues 192-215) that connects apoE's N- and C-terminal domains may play an important role in mediating this domain-domain interaction.
