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1.
Molecules ; 26(22)2021 Nov 19.
Article in English | MEDLINE | ID: mdl-34834086

ABSTRACT

Diverse medicinal plants such as those from the genus Artemisia have been employed globally for centuries by individuals belonging to different cultures. Universally, Artemisia species have been used to remedy various maladies that range from simple fevers to malaria. A survey conducted by the World Health Organization (WHO) demonstrated that 80% of the global population is highly reliant on herbal medicine for their primary healthcare. WHO recommends artemisinin-based combination therapies (ACT) for the treatment of global diseases such as malaria. Artemisinin is a bioactive compound derived from Artemisia annua leaves. It is a sesquiterpene endoperoxide with potent antimalarial properties. This review strives to instill natural products to chemists and others in diverse fields with a heterogeneous set of knowledge compiled from multifaceted researchers and organizations in literature. In particular, the various Artemisia species and effective extraction, isolation, and characterization methodologies are discussed in detail. An in-depth investigation into the literature reveals that divergent species of Artemisia exhibit a vast array of biological activities such as antimalarial, antitumor, and anti-inflammatory activities. There is substantial potential for bioactive compounds from Artemisia to provide significant relief from differing human ailments, but more meticulous research in this field is needed.


Subject(s)
Artemisia annua/chemistry , Artemisinins , Malaria/drug therapy , Phytochemicals , Plants, Medicinal/chemistry , Artemisinins/chemistry , Artemisinins/isolation & purification , Artemisinins/therapeutic use , Humans , Phytochemicals/chemistry , Phytochemicals/isolation & purification , Phytochemicals/therapeutic use
2.
Curr Drug Metab ; 22(11): 858-869, 2021.
Article in English | MEDLINE | ID: mdl-33511947

ABSTRACT

BACKGROUND: As parasite resistance to the main artemisinin drugs has emerged in Southern Asia, the traditional herb Artemisia annua L. (AAL) from which artemisinin (QHS) isolated was found to overcome resistance to QHS. However the component and metabolite profiles of AAL remain unclear. OBJECTIVE: In this study, component profiling of marker compounds in AAL (amorphane sesquiterpene lactones and flavonoids) was performed and their subsequent metabolism was investigated in rats. METHODS: For efficient component classification and structural characterization, an improved liquid chromatography- tandem high-resolution mass spectrometry (HRMS)-based analytical strategy was applied, i.e., background subtraction (BS) followed by ring-double-bond (RDB) filter in tandem with repeated BS processing. Structures of detected components/metabolites were characterized based on integrated information including their HRMSn patterns, RDB values, the established component/metabolite network, the biosynthesis pathways of AAL, and/or NMR data. RESULTS: A total of 38 amorphane sesquiterpene lactones and 35 flavonoids were found in AAL as prototype compounds, among which 26 components were previously undescribed. Major compounds were identified by comparing them with reference standards. Among 73 AAL prototypes administered, 38 were absorbed in the circulation as the prototype. Moreover, 20 metabolites of amorphane sesquiterpene lactones and 10 metabolites of flavonoids were detected in rats. The major metabolic pathways included oxidation, methylation, glucuronidation and sulfation. CONCLUSION: The component and metabolite network were established for marker components in AAL, which will be valuable to understand the synergistic antimalarial potency of QHS in A. annua L. The analytical strategy can also be applied to other herbal medicines.


Subject(s)
Artemisia annua/chemistry , Gas Chromatography-Mass Spectrometry/methods , Sesquiterpenes/pharmacokinetics , Animals , Artemisinins/isolation & purification , Artemisinins/metabolism , Artemisinins/pharmacokinetics , Lactones/isolation & purification , Lactones/metabolism , Lactones/pharmacokinetics , Male , Rats , Rats, Wistar , Sesquiterpenes/isolation & purification , Sesquiterpenes/metabolism
3.
J Ethnopharmacol ; 268: 113638, 2021 Mar 25.
Article in English | MEDLINE | ID: mdl-33271239

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia annua has a long history of use in Southeast Asia where it was used to treat "fever", and A. afra has a similar history in southern Africa. Since their discovery, A. annua use, in particular, has expanded globally with millions of people using the plant in therapeutic tea infusions, mainly to treat malaria. AIM OF THE STUDY: In this study, we used in vitro studies to query if and how A. annua and A. afra tea infusions being used across the globe affect asexual Plasmodium falciparum parasites, and their sexual gametocytes. MATERIALS AND METHODS: P. falciparumstrain NF54 was grown in vitro, synchronized, and induced to form gametocytes using N-acetylglucosamine. Cultures during asexual, early, and late stage gametocytogenesis were treated with artemisinin, methylene blue, and A. annua and A. afra tea infusions (5 g DW/L) using cultivars that contained 0-283 µM artemisinin. Asexual parasitemia and gametocytemia were analyzed microscopically. Gametocyte morphology also was scored. Markers of early (PfGEXP5) and late stage (Pfs25) gametocyte gene expression also were measured using RT-qPCR. RESULTS: Both A. annua and A. afra tea infusions reduced gametocytemia in vitro, and the effect was mainly artemisinin dependent. Expression levels of both marker genes were reduced and also occurred with the effect mainly attributed to artemisinin content of four tested Artemisia cultivars. Tea infusions of both species also inhibited asexual parasitemia and although mainly artemisinin dependent, there was a weak antiparasitic effect from artemisinin-deficient A. afra. CONCLUSIONS: These results showed that A. annua and to a lesser extent, A. afra, inhibited parasitemia and gametocytemia in vitro.


Subject(s)
Artemisia , Artemisinins/pharmacology , Plant Extracts/pharmacology , Plasmodium falciparum/drug effects , Tea , Artemisinins/isolation & purification , Germ Cells/drug effects , Germ Cells/physiology , Plant Extracts/isolation & purification , Plasmodium falciparum/physiology
4.
Pharmacol Ther ; 216: 107658, 2020 12.
Article in English | MEDLINE | ID: mdl-32777330

ABSTRACT

As the first-line antimalarial drugs, artemisinins gained wide acceptance after the emergence of resistance to chloroquine in the 1950s. Artemisinin-based drugs have saved lives, especially in developing countries. The discovery of artemisinin was unique, timely, and fascinating, and the benefits of artemisinin were with far-reaching implications. Herein, we will give a brief description of various aspects of the development of artemisinin and discuss the position and perspectives of artemisinin-based drugs.


Subject(s)
Antimalarials/therapeutic use , Artemisia annua , Artemisinins/therapeutic use , Malaria/drug therapy , Plasmodium/drug effects , Animals , Antimalarials/chemistry , Antimalarials/isolation & purification , Artemisia annua/chemistry , Artemisinins/chemistry , Artemisinins/isolation & purification , Humans , Malaria/parasitology , Molecular Structure , Plasmodium/pathogenicity , Structure-Activity Relationship
5.
Carbohydr Polym ; 246: 116590, 2020 Oct 15.
Article in English | MEDLINE | ID: mdl-32747249

ABSTRACT

Artemisinin is mainly derived from Artemisia annua L. Since the leaves composition is complex, artemisinin purification faces great challenges. In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity. The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification. Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process. The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g. In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied. The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.


Subject(s)
Artemisinins/isolation & purification , Chitosan/chemistry , Plant Leaves/chemistry , Plant Oils/isolation & purification , Silanes/chemistry , Solid Phase Extraction/methods , Adsorption , Artemisia annua/chemistry , Crystallization , Humans , Hydrogen Bonding , Hydrolysis , Hydrophobic and Hydrophilic Interactions , Kinetics , Membranes, Artificial , Plant Extracts/chemistry , Plant Oils/chemistry , Static Electricity , Waxes/chemistry
6.
Plant Cell Rep ; 39(1): 101-117, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31576412

ABSTRACT

KEY MESSAGE: Blue and yellow light affected metabolism and the morphology. Blue and red promote the DOXP/MEP pathway. ADS gene expression was increased in plants cultivated under blue, promoting artemisinin content. Artemisinin-based combination therapies are the most effective treatment for highly lethal malaria. Artemisinin is produced in small quantities in the glandular trichomes of Artemisia annua L. Our aim was to evaluate the effect of light quality in A. annua cultivated in vitro under different light qualities, considering anatomical and morphological changes, the volatile composition, artemisinin content and the expression of two key enzymes for artemisinin biosynthesis. Yellow light is related to the increase in the number of glandular trichomes and this seemed to positively affect the molecular diversity in A. annua. Yellow light-stimulated glandular trichome frequency without triggered area enhancement, whereas blue light stimulated both parameters. Blue light enhanced the thickness of the leaf epidermis. The B-promoting effect was due to increased cell size and not to increased cell numbers. Green and yellow light positively influenced the volatile diversity in the plantlets. Nevertheless, blue and red light seemed to promote the DOXP/MEP pathway, while red light stimulates MVA pathway. Amorpha-4,11-diene synthase gene expression was significantly increased in plants cultivated under blue light, and not red light, promoting artemisinin content. Our results showed that light quality, more specifically blue and yellow light, positively affected secondary metabolism and the morphology of plantlets. It seemed that steps prior to the last one in the artemisinin biosynthesis pathway could be strongly influenced by blue light. Our work provides an alternative method to increase the amount of artemisinin production in A. annua without the use of transgenic plants, by the employment of blue light.


Subject(s)
Artemisia annua/metabolism , Artemisinins/metabolism , Plant Leaves/chemistry , Plant Leaves/metabolism , Artemisinins/isolation & purification , Biosynthetic Pathways , Gene Expression Regulation, Plant , Light , Plant Leaves/ultrastructure , Plant Proteins/genetics , Secondary Metabolism , Trichomes/metabolism
7.
Future Med Chem ; 11(12): 1443-1459, 2019 06.
Article in English | MEDLINE | ID: mdl-31298579

ABSTRACT

Artemisinin (ART) is an endoperoxide sesquiterpene lactone, commonly used in the treatment of malaria. Although it was isolated from Artemisia annuaL., a plant widely applied in Chinese Traditional Medicine, its mechanism of action remains uncertain and its clinical use is still limited due to its low solubility, its poor bioavailability and short in vivo half-life. Over time, several studies have been aimed towards the discovery of potent ART derivatives that could overcome clinical drawbacks. In this review, we focus on the multifaced aspects of ART and on the efforts spent to improve its pharmacological profile that so far culminated in the discovery of more effective drugs. Lastly, we outline the new perspectives in the ART-derivatives scenario.


Subject(s)
Antimalarials/chemical synthesis , Artemisia/chemistry , Artemisinins/chemical synthesis , Malaria/drug therapy , Medicine, Chinese Traditional , Antimalarials/chemistry , Antimalarials/isolation & purification , Antimalarials/therapeutic use , Artemisinins/chemistry , Artemisinins/isolation & purification , Artemisinins/therapeutic use , Humans , Molecular Structure
8.
Phytochem Anal ; 30(5): 564-571, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31238388

ABSTRACT

INTRODUCTION: Artemisia annua is a small herbaceous plant belonging to the Asteraceae family declared therapeutic by the World Health Organisation, in particular for its artemisinin content, an active ingredient at the base of most antimalarial treatments, used every year by over 300 million people. In the last years, owing to low artemisinin content, research of new ways to increase the yield of the plant matrix has led to the use of the total extract taking advantage from the synergic and stabilising effects of the other components. OBJECTIVE: In this work we evaluated and compared the content of artemisinin and scopoletin in extracts of A. annua collected in the Campania Region (southern Italy), by two different extraction processes. METHODOLOGY: Artemisia annua plants were extracted by traditional maceration (TM) in hydroalcoholic solution as a mother tincture prepared according to the European Pharmacopeia and by pressurised cyclic solid-liquid (PCSL) extraction, a new generation method using the Naviglio extractor. RESULTS: The results showed that the PCSL extraction technique is more effective than traditional methods in extracting both phytochemicals, up to 15 times more, reducing the extraction times, without using solvents or having risks for the operators, the environment and the users of the extracts. CONCLUSION: The Naviglio extractor provides extracts with an artemisinin and scopoletin content eight times higher than the daily therapeutic dose, which should be evaluated for its stability over time and biological properties for possible direct use for therapeutic purposes.


Subject(s)
Artemisia annua/chemistry , Artemisinins/isolation & purification , Liquid-Liquid Extraction/methods , Plant Extracts/chemistry , Scopoletin/isolation & purification , Solid Phase Extraction/methods , Pressure
9.
Ticks Tick Borne Dis ; 9(5): 1115-1119, 2018 07.
Article in English | MEDLINE | ID: mdl-29693549

ABSTRACT

The present study evaluated whether a natural dietary additive, dried Artemisia annua leaves, may be useful to control Rhipicephalus (Boophilus) microplus on naturally infested cattle. Twenty heifers of the Canchim breed, weighing around 250 kg, were divided into two equally sized experimental groups: 1) control animals and 2) animals receiving 200 g/day of dried A. annua leaves for two months. Before treatment began, the animals were homogeneously distributed in control and treatment groups based on their pre-treatment weight and tick infestation level. Counts of engorged female ticks then occurred weekly during the two-month experimental period. We also monitored cattle weight gain and packed cell volume (PCV). Artemisinin (0.96%) was quantified in the plant material by high-performance liquid chromatography with refractive index detector (HPLC-IR). No statistical differences between the control and treatment groups were observed for engorged female counts (log averages of 1.3 ticks and 1.4 ticks per animal, respectively), daily cattle weight gain (0.910 kg and 0.888 kg, respectively) or PCV (33.5% and 33.0%, respectively). We conclude that the oral supplementation of cattle feed with dried A. annuna leaves did not control natural infestation of R. (B.) microplus. The hypothesis of artemisinin's action on cattle ticks by ingestion through the animals' blood was not confirmed at the evaluated dose.


Subject(s)
Artemisia annua/anatomy & histology , Artemisinins/administration & dosage , Cattle Diseases/diet therapy , Food Additives/administration & dosage , Rhipicephalus/drug effects , Tick Infestations/veterinary , Acaricides/therapeutic use , Animal Feed/analysis , Animals , Artemisia annua/chemistry , Artemisinins/analysis , Artemisinins/isolation & purification , Cattle , Female , Food, Preserved , Male , Plant Leaves/chemistry , Tick Infestations/diet therapy , Weight Gain/drug effects
10.
Mol Biotechnol ; 60(2): 169-183, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29290031

ABSTRACT

Plant secondary metabolism evolved in the context of highly organized and differentiated cells and tissues, featuring massive chemical complexity operating under tight environmental, developmental and genetic control. Biotechnological demand for natural products has been continuously increasing because of their significant value and new applications, mainly as pharmaceuticals. Aseptic production systems of plant secondary metabolites have improved considerably, constituting an attractive tool for increased, stable and large-scale supply of valuable molecules. Surprisingly, to date, only a few examples including taxol, shikonin, berberine and artemisinin have emerged as success cases of commercial production using this strategy. The present review focuses on the main characteristics of plant specialized metabolism and their implications for current strategies used to produce secondary compounds in axenic cultivation systems. The search for consonance between plant secondary metabolism unique features and various in vitro culture systems, including cell, tissue, organ, and engineered cultures, as well as heterologous expression in microbial platforms, is discussed. Data to date strongly suggest that attaining full potential of these biotechnology production strategies requires being able to take advantage of plant specialized metabolism singularities for improved target molecule yields and for bypassing inherent difficulties in its rational manipulation.


Subject(s)
Biological Products/metabolism , Biotechnology/methods , Metabolic Engineering/methods , Phytochemicals/biosynthesis , Plant Cells/metabolism , Plants/metabolism , Artemisinins/isolation & purification , Artemisinins/metabolism , Axenic Culture , Berberine/isolation & purification , Berberine/metabolism , Biological Products/isolation & purification , Cell Culture Techniques , Naphthoquinones/isolation & purification , Naphthoquinones/metabolism , Paclitaxel/biosynthesis , Paclitaxel/isolation & purification , Phytochemicals/isolation & purification , Plant Cells/chemistry , Plants/chemistry , Plants/genetics , Secondary Metabolism , Tissue Culture Techniques
11.
Chem Pharm Bull (Tokyo) ; 66(3): 319-326, 2018 Mar 01.
Article in English | MEDLINE | ID: mdl-29311435

ABSTRACT

In order to make full use of artemisinin production waste and thus to reduce the production cost of artemisinin, we developed an efficient and scalable method to isolate high-purity dihydroartemisinic acid from artemisinin production waste by combining anion-exchange resin with silica-gel column chromatography. The adsorption and desorption characteristics of dihydroartemisinic acid on 10 types of anion-exchange resin were investigated, and the results showed that the 717 anion-exchange resin exhibited the highest capacity of adsorption and desorption to dihydroartemisinic acid. Adsorption isotherms were established for the 717 anion-exchange resin and they fitted well with both Langmuir and Freundlich model. Dynamic adsorption and desorption properties of 717 anion-exchange resin were characterized to optimize the chromatographic conditions. Subsequently, the silica-gel column chromatography was performed and dihydroartemisinic acid with a purity of up to 98% (w/w) was obtained. Finally, the scale-up experiments validated the preparative separation of high-purity dihydroartemisinic acid from industrial waste developed in the present work. This work presented for the first time an isolation of dihydroartemisinic acid with a purity of 98% from Artemisia annua (A. annua) by-product, which adds more value to this crop and has the potential to lower the prices of anti-malarial drugs.


Subject(s)
Antimalarials/chemistry , Antimalarials/isolation & purification , Artemisinins/chemistry , Artemisinins/isolation & purification , Adsorption , Artemisia annua/chemistry , Chromatography, Liquid , Kinetics , Medical Waste , Solvents
12.
Anal Chem ; 90(5): 3109-3117, 2018 03 06.
Article in English | MEDLINE | ID: mdl-29381342

ABSTRACT

A fast, simple, efficient, and high-throughput analytical protocol using deep eutectic solvents (DES) for mechanochemical extraction (MCE) combined with direct analysis in real time mass spectrometry (DART-MS) was developed to quantify heat-labile bioactive compounds artemisinin (AN), arteannuin B, and artemisinic acid from Aretemisia annua. MCE is performed at room temperature, and target analytes are released into DESs within seconds; this method demonstrated multiple advantages over traditional extraction methods and organic solvents. DART-MS was then used for the structure confirmation and quantification for the three artemisinin major components extracted from plants of five locations. Liquid chromatography (LC) measurements were performed as well for results verification and comparison, and the amounts obtained were consistent between the two techniques. DART-MS showed advantages in simplicity, low limit of detection (5-15 ng mL-1), and superior speed (10-20 s), but with slightly higher relative standard deviation (0.7-10.8%). The entire protocol can be accomplished in a few minutes from raw materials to quantitative results. This study aims to establish a methodology combining high-efficiency sample pretreatment and rapid chemical analysis from complex matrixes, where the time-consuming separation procedure can be eliminated. Additionally, the use of toxic organic solvents needed in the process of chemical extraction and analysis is largely avoided. In general, this investigation provides a robust analytical procedure that can be widely used in many areas of research and industrial activities.


Subject(s)
Artemisinins/analysis , Mass Spectrometry/methods , Solid Phase Extraction/methods , Solvents/chemistry , Artemisia annua/chemistry , Artemisinins/isolation & purification , Limit of Detection , Solvents/chemical synthesis
13.
J Ethnopharmacol ; 207: 86-91, 2017 Jul 31.
Article in English | MEDLINE | ID: mdl-28642094

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: The chemical matrix of the herb Artemisia annua L. (A. annua), from which artemisinin (QHS) is isolated, can enhance both the bioavailability and efficacy of QHS. However, the exact mechanism of this synergism remains unknown. The biotransformation of QHS and potential "enzyme inhibitors" in plant matrix could be of great importance in understanding the improved efficacy of QHS in A. annua, which has been limited to the synergism with flavonoid components. AIM OF THE STUDY: To investigate the component in A. annua extracts (MAE) leading to enhanced antiplasmodial potency of QHS via regulation of its metabolism. The efficacy of QHS in combination with the synergistic component was also evaluated. MATERIALS AND METHODS: The total MAE extract and its three MAE fractions (MAE-I eluted using 3% methanol, MAE-II eluted using 50% methanol and MAE-III eluted using 85% methanol) were obtained from dry plant materials and prepared after lyophilization. The pharmacokinetic profiles of QHS and its major phase I metabolite monohydroxylated artemisinin (QHS-M) were investigated in healthy rats after a single oral administration of QHS in each MAE extract. Major components isolated from the target MAE fraction were evaluated for their enzyme inhibition. The antimalarial activity of QHS in combination with the potential synergistic component against Plasmodium falciparum was studied in vivo (murine Plasmodium yoelii). The recrudescence and survival time of infected mice were also recorded after drug treatment. RESULTS: Compared to pure QHS, a 2-fold increase in QHS exposure (AUC and Cmax) was found in healthy rats after a single oral dose of QHS in the total MAE extract or its fraction MAE-III. In addition, metabolic biotransformation of QHS to the metabolite QHS-M (mediated by CYP3A) was inhibited by MAE or MAE-III. Among nine major components isolated from MAE-III (five sesquiterpenenes, three flavonoids and one phenolic acid), only arteannuin B (AB) showed an inhibition of CYP3A4 (IC50 1.2µM). The synergism between QHS and AB was supported using in vivo antiplasmodial assay and a pharmacokinetic study in mice. Unfortunately, the synergism cannot reduce the rate of recrudescence. CONCLUSIONS: AB was one of main contributors in A. annua leading to enhanced antiplasmodial potency of QHS via regulation of its metabolism. The final recrudescence indicated the careful use of A. annua for malaria treatment unless additional contributing components or antiplasmodial mechanism were found.


Subject(s)
Antimalarials/pharmacology , Artemisia annua/chemistry , Artemisinins/pharmacology , Plant Extracts/pharmacology , Animals , Antimalarials/isolation & purification , Antimalarials/pharmacokinetics , Area Under Curve , Artemisinins/isolation & purification , Artemisinins/pharmacokinetics , Biological Availability , Drug Synergism , Flavonoids/isolation & purification , Flavonoids/pharmacology , Inhibitory Concentration 50 , Malaria, Falciparum/drug therapy , Male , Mice , Mice, Inbred ICR , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Plasmodium falciparum/drug effects , Rats , Rats, Wistar
14.
Chem Pharm Bull (Tokyo) ; 65(8): 746-753, 2017 Aug 01.
Article in English | MEDLINE | ID: mdl-28566563

ABSTRACT

Malaria is the most devastating parasitic disease worldwide. Artemisinin is the only drug that can cure malaria that is resistant to quinine-derived drugs. After the commercial extraction of artemisinin from Artemisia annua, the recovery of dihydroartemisinic acid (DHAA) from artemisinin extraction by-product has the potential to increase artemisinin commercial yield. Here we describe the development and optimization of an ultrasound-assisted alkaline procedure for the extraction of DHAA from artemisinin production waste using response surface methodology. Our results using this methodology established that NaOH at 0.36%, extraction time of 67.96 min, liquid-solid ratio of 5.89, and ultrasonic power of 83.9 W were the optimal conditions to extract DHAA from artemisinin production waste. Under these optimal conditions, we achieved a DHAA yield of 2.7%. Finally, we conducted a validation experiment, and the results confirmed the prediction generated by the regression model developed in this study. This work provides a novel way to increase the production of artemisinin per cultivated area and to reduce artemisinin production costs by recycling its commercial waste to obtain DHAA, an immediate precursor of artemisinin. The use of this technology may reduce the costs of artemisinin-based antimalarial medicines.


Subject(s)
Artemisia annua/chemistry , Artemisinins/isolation & purification , Ultrasonics , Artemisinins/chemistry , Hydrogen-Ion Concentration , Regression Analysis , Sodium Hydroxide/chemistry , Surface Properties
15.
Ultrason Sonochem ; 34: 310-316, 2017 01.
Article in English | MEDLINE | ID: mdl-27773251

ABSTRACT

This study proposes an ultrasound-horn system for the extraction of a natural active compound "artemisinin" from Artemisia annua L. leaves as an alternative to hot maceration technique. Ultrasound leaching improves artemisinin recovery at all temperatures where only ten minutes is required to recover 70% (4.42mgg-1) compared to 60min of conventional hot leaching for the same yield. For instance, ultrasound treatment at 30°C produced a higher yield than the one obtained by conventional maceration at 40°C. Kinetic study suggests that the extraction pattern can be assimilated, during the first ten minutes, to a first order steady state, from which activation energy calculations revealed that each gram of artemisinin required 7.38kJ in ultrasound versus 10.3kJ in the conventional system. Modeling results indicate the presence of two extraction stages, a faster stage with a diffusion coefficient of 19×10-5cm2min-1 for ultrasound technique at 40°C, seven times higher than the conventional one; and a second deceleration stage similar for both techniques with diffusion coefficient ranging from 1.7 to 3.1×10-5cm2min-1. It is noted that the efficient ultrasound extraction potential implies extraction of higher amount of co-metabolites so low artemisinin crystal purity is engendered but a combination with a purification step using activated charcoal and celite adsorbents produced crystals with comparable purity for conventional and ultrasound samples.


Subject(s)
Artemisia annua/chemistry , Artemisinins/isolation & purification , Chemical Fractionation/methods , Plant Leaves/chemistry , Ultrasonic Waves , Adsorption , Artemisinins/chemistry , Temperature
16.
Genet Mol Res ; 15(3)2016 Aug 26.
Article in English | MEDLINE | ID: mdl-27706602

ABSTRACT

The anti-malarial drug, artemisinin, is quite expensive as a result of its slow content in Artemisia annua. Recent investigations have suggested that genetic engineering of A. annua is a promising approach to improve the yield of artemisinin. In this study, the transgenic A. annua strain GYR, which has high artemisinin content, was evaluated in an environmental release trial. First, GYR plants were compared with the wild-type variety NON-GYR, with regard to phenotypic characters (plant height, crown width, stem diameter, germination rate, leaf dry weight, 1000-seed weight, leave shape). Second, stress resistance in the two varieties (salt, drought, herbicide, and cold resistance) was evaluated under different experimental conditions. Finally, gene flow was estimated. The results indicated that there were significant differences in several agronomic traits (plant height, stem diameter, and leave dry weight) between the transgenic GYR and NON-GYR plants. Salt stress in transgenic and control plants was similar, except under high NaCl concentrations (1.6%, w/w). Leaf water, proline, and MDA content (increased significantly) were significantly different. Transgenic A. annua GYR plants did not grow better than NON-GYR plants with respect to drought and herbicide resistance. The two varieties maintained vitality through the winter. Third, gene flow was studied in an environmental risk trial for transgenic GYR. The maximum gene flow frequency was 2.5%, while the maximum gene flow distance was 24.4 m; gene flow was not detected at 29.2 m at any direction. Our findings may provide an opportunity for risk assessment in future commercialization of transgenic A. annua varieties.


Subject(s)
Antimalarials/metabolism , Artemisia annua/genetics , Artemisinins/metabolism , Gene Expression Regulation, Plant , Plant Leaves/genetics , Plants, Genetically Modified , Adaptation, Physiological/genetics , Antimalarials/isolation & purification , Artemisia annua/metabolism , Artemisinins/isolation & purification , Cold Temperature , Droughts , Gene Flow , Genetic Engineering , Germination/genetics , Hot Temperature , Malondialdehyde/metabolism , Phenotype , Plant Leaves/metabolism , Proline/metabolism , Salinity , Stress, Physiological
17.
Metab Eng ; 38: 159-169, 2016 11.
Article in English | MEDLINE | ID: mdl-27421621

ABSTRACT

Our lack of full understanding of transport and sequestration of the heterologous products currently limit metabolic engineering in plants for the production of high value terpenes. For instance, although all genes of the artemisinin/arteannuin B (AN/AB) biosynthesis pathway (AN-PW) from Artemisia annua have been identified, ectopic expression of these genes in Nicotiana benthamiana yielded mostly glycosylated pathway intermediates and only very little free (dihydro)artemisinic acid [(DH)AA]. Here we demonstrate that Lipid Transfer Protein 3 (AaLTP3) and the transporter Pleiotropic Drug Resistance 2 (AaPDR2) from A. annua enhance accumulation of (DH)AA in the apoplast of N. benthamiana leaves. Analysis of apoplast and cell content and apoplast exclusion assays show that AaLTP3 and AaPDR2 prevent reflux of (DH)AA from the apoplast back into the cells and enhances overall flux through the pathway. Moreover, AaLTP3 is stabilized in the presence of AN-PW activity and co-expression of AN-PW+AaLTP3+AaPDR2 genes yielded AN and AB in necrotic N. benthamiana leaves at 13 days post-agroinfiltration. This newly discovered function of LTPs opens up new possibilities for the engineering of biosynthesis pathways of high value terpenes in heterologous expression systems.


Subject(s)
Artemisia annua/physiology , Artemisinins/metabolism , Biosynthetic Pathways/physiology , Carrier Proteins/metabolism , Metabolic Engineering/methods , Nicotiana/physiology , Plant Proteins/metabolism , Artemisinins/isolation & purification , Carrier Proteins/genetics , Genetic Enhancement/methods , Metabolic Networks and Pathways/physiology , Plant Proteins/genetics
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