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1.
Acta Pharmacol Sin ; 41(8): 1073-1084, 2020 Aug.
Article in English | MEDLINE | ID: mdl-32139897

ABSTRACT

Serum- and glucocorticoid-inducible kinease-1 (SGK1) is a serine/threonine kinase regulated by hypotonic stimuli, which is involved in regulation of cell cycle and apoptosis. Our previous study shows that activation of volume-regulated Cl- channels (VRCCs) protects rat basilar artery smooth muscle cells (BASMCs) against hydrogen peroxide (H2O2)-induced apoptosis. In the present study, we investigated whether SGK1 was involved in the protective effect of VRCCs in BASMCs. We showed that hypotonic challenge significantly reduced H2O2-induced apoptosis, and increased SGK1 phosphorylation, but did not affect SGK1 protein expression. The protective effect of hypotonic challenge against H2O2-induced apoptosis was mediated through inhibiting mitochondria-dependent apoptotic pathway, evidenced by increased Bcl-2/Bax ratio, stabilizing mitochondrial membrane potential (MMP), decreased cytochrome c release from the mitochondria to the cytoplasm, and inhibition of the activation of caspase-9 and caspase-3. These protective effects of hypotonic challenge against H2O2-induced apoptosis was diminished and enhanced, respectively, by SGK1 knockdown and overexpression. We further revealed that SGK1 activation significantly increased forkhead box O3a (FOXO3a) phosphorylation, and then inhibited the translocation of FOXO3a into nucleus and the subsequent expression of Bcl-2 interacting mediator of cell death (Bim). In conclusion, SGK1 mediates the protective effect of VRCCs against H2O2-induced apoptosis in BASMCs via inhibiting FOXO3a/Bim signaling pathway. Our results provide compelling evidences that SGK1 is a critical link between VRCCs and apoptosis, and shed a new light on the treatment of vascular apoptosis-associated diseases, such as vascular remodeling, angiogenesis, and atherosclerosis.


Subject(s)
Apoptosis/drug effects , Chloride Channels/physiology , Hydrogen Peroxide/pharmacology , Immediate-Early Proteins/physiology , Osmotic Pressure/physiology , Protein Serine-Threonine Kinases/physiology , Signal Transduction/physiology , Animals , Basilar Artery/cytology , Bcl-2-Like Protein 11/metabolism , Down-Regulation , Forkhead Box Protein O3/metabolism , Male , Myocytes, Smooth Muscle , Rats, Sprague-Dawley
2.
J Cell Mol Med ; 21(9): 1826-1834, 2017 09.
Article in English | MEDLINE | ID: mdl-28294531

ABSTRACT

The present study was designed to investigate whether large conductance Ca2+ -activated K+ (BK) channels were regulated by epidermal growth factor (EGF) receptor (EGFR) tyrosine kinase. BK current and channel tyrosine phosphorylation level were measured in BK-HEK 293 cells expressing both functional α-subunits and the auxiliary ß1-subunits using electrophysiology, immunoprecipitation and Western blotting approaches, respectively, and the function of rat cerebral basilar arteries was determined with a wire myography system. We found that BK current in BK-HEK 293 cells was increased by the broad spectrum protein tyrosine kinase (PTK) inhibitor genistein and the selective EGFR tyrosine kinase inhibitor AG556, one of the known tyrphostin. The effect of genistein or AG556 was antagonized by the protein tyrosine phosphatase (PTP) inhibitor orthovanadate. On the other hand, orthovanadate or EGF decreased BK current, and the effect was counteracted by AG556. The tyrosine phosphorylation level of BK channels (α- and ß1-subunits) was increased by EGF and orthovanadate, while decreased by genistein and AG556, and the reduced tyrosine phosphorylation of BK channels by genistein or AG556 was reversed by orthovanadate. Interestingly, AG556 induced a remarkable enhancement of BK current in rat cerebral artery smooth muscle cells and relaxation of pre-contracted rat cerebral basilar arteries with denuded endothelium, and these effects were antagonized by the BK channel blocker paxilline or orthovanadate. These results demonstrate that tyrosine phosphorylation of BK channels by EGFR kinase decreases the channel activity, and inhibition of EGFR kinase by AG556 enhances the channel activity and dilates rat cerebral basilar arteries.


Subject(s)
ErbB Receptors/antagonists & inhibitors , Large-Conductance Calcium-Activated Potassium Channels/metabolism , Tyrphostins/pharmacology , Animals , Basilar Artery/cytology , Cell Separation , Epidermal Growth Factor/pharmacology , ErbB Receptors/metabolism , Genistein/pharmacology , HEK293 Cells , Humans , Indoles/pharmacology , Ion Channel Gating/drug effects , Male , Myocytes, Smooth Muscle/drug effects , Myocytes, Smooth Muscle/metabolism , Phosphorylation/drug effects , Phosphotyrosine/metabolism , Protein Subunits/metabolism , Rats, Sprague-Dawley , Vanadates/pharmacology , Vasodilation/drug effects
3.
Mol Med Rep ; 13(4): 3691-9, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26955761

ABSTRACT

Calcium activated chloride channels (CaCCs) are critical in vascular smooth muscle function as they regulate proliferation/apoptosis of smooth muscle cells (SMCs) and vascular tone. Transmembrane protein 16A (TMEM16A) was demonstrated to encode CaCCs in basilar artery SMCs (BASMCs) and participate in basilar artery remodeling during hypertension. In addition, TMEM16A has recently been illustrated to contribute to pressure­induced myogenic response in cerebral vasculature. However, whether TMEM16A is involved in cerebral vasoconstriction that is stimulated by other vasoconstrictors remains unclear. The aim of the present study was to establish whether TMEM16A is involved in the progression of angiotensin II (Ang II)­induced basilar artery constriction and elucidate its potential role during hypertension. The study demonstrated that the specific inhibitor of TMEM16A, T16A­inhA01 attenuated Ang II­induced constriction in rat basilar arteries, and that this effect was weakened in parallel with the decline of TMEM16A expression in basilar arteries of 2­kidney, 2­clip hypertensive rats. Furthermore, it was found that 100 nM Ang II evoked a chloride current in cultured BASMCs with a basal 100­nM intracellular Ca2+ ([Ca2+]i) level. In addition, the current could be abolished by TMEM16A small interfering RNA pretreatment and Ang II receptor type 1 (AT1) receptor blocker, losartan, while Ang II failed to cause a further increase to Ca2+­dependent Cl­ currents activated by 500 nM [Ca2+]i. In addition, in cultured BASMCs, Ang II induced phosphorylation of myosin phosphatase­targeting subunit 1, and myosin light chains were significantly enhanced by TMEM16A overexpression, which were reversed by Rho­associated protein kinase (ROCK) inhibitor, Y­27632, while TMEM16A silencing demonstrated an opposing result. Furthermore, Ang II­induced RhoA activation was enhanced by TMEM16A overexpression. In conclusion, the present study revealed that Ang II elicited a TMEM16A­mediated current and TMEM16A participated in Ang II­induced basilar constriction via the RhoA/ROCK signaling pathway.


Subject(s)
Angiotensin II/toxicity , Chloride Channels/metabolism , Muscle, Smooth, Vascular/metabolism , Signal Transduction/drug effects , rho-Associated Kinases/metabolism , rhoA GTP-Binding Protein/metabolism , Amides/pharmacology , Angiotensin Receptor Antagonists/pharmacology , Animals , Anoctamin-1 , Basilar Artery/cytology , Cells, Cultured , Chloride Channels/antagonists & inhibitors , Chloride Channels/genetics , Disease Models, Animal , Evoked Potentials/drug effects , Hypertension/etiology , Hypertension/pathology , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Protein Phosphatase 1/metabolism , Pyridines/pharmacology , Rats , Rats, Sprague-Dawley , Vasoconstriction/drug effects , rho-Associated Kinases/antagonists & inhibitors , rhoA GTP-Binding Protein/antagonists & inhibitors
4.
Neurotoxicology ; 53: 282-289, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26945730

ABSTRACT

Mercury (Hg) has many harmful vascular effects by increasing oxidative stress, inflammation and vascular/endothelial dysfunction, all of which may contribute to cerebrovascular diseases development. We aimed to explore the effects of chronic low-mercury concentration on vascular function in cerebral arteries and the mechanisms involved. Basilar arteries from control (vehicle-saline solution, im) and mercury chloride (HgCl2)-treated rats for 30 days (first dose 4.6µg/kg, subsequent dose 0.07µg/kg/day, im, to cover daily loss) were used. Vascular reactivity, protein expression, nitric oxide (NO) levels and superoxide anion (O2(-)) production were analyzed. HgCl2 exposure increased serotonin contraction and reduced the endothelium-dependent vasodilatation to bradykinin. After NO synthase inhibition, serotonin responses were enhanced more in control than in mercury-treated rats while bradykinin-induced relaxation was abolished. NO levels were greater in control than Hg-treated rats. Tiron and indomethacin reduced vasoconstriction and increased the bradykinin-induced relaxation only in HgCl2-treated rats. Vascular O2(-) production was greater in mercury-treated when compared to control rats. Protein expressions of endothelial NO synthase, copper/zinc (Cu/Zn), Manganese (Mn) and extracellular-superoxide dismutases were similar in cerebral arteries from both groups. Results suggest that Hg treatment increases cerebrovascular reactivity by reducing endothelial negative modulation and NO bioavailability; this effect seems to be dependent on increased reactive oxygen species and prostanoids generation. These findings show, for the first time, that brain vasculature are also affected by chronic mercury exposure and offer further evidence that even at small concentration, HgCl2 is hazardous and might be an environmental risk factor accounting for cerebral vasospasm development.


Subject(s)
Cerebrovascular Disorders/chemically induced , Cerebrovascular Disorders/pathology , Endothelial Cells/drug effects , Mercuric Chloride/pharmacology , Vasoconstriction/drug effects , Analysis of Variance , Animals , Basilar Artery/cytology , Body Weight/drug effects , Bradykinin/pharmacology , Dose-Response Relationship, Drug , Drinking/drug effects , Eating/drug effects , Hydrazines/pharmacology , Male , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide/metabolism , Nitric Oxide Donors/pharmacology , Rats , Rats, Wistar , Serotonin/pharmacology , Superoxides/metabolism , Vasodilator Agents/pharmacology
5.
Br J Pharmacol ; 173(3): 529-44, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26562480

ABSTRACT

BACKGROUND AND PURPOSE: Angiotensin II (AngII) induces migration and growth of vascular smooth muscle cell (VSMC), which is responsible for vascular remodelling in some cardiovascular diseases. Ang II also activates a Cl(-) current, but the underlying mechanism is not clear. EXPERIMENTAL APPROACH: The A10 cell line and primary cultures of VSMC from control, ClC-3 channel null mice and WT mice made hypertensive with AngII infusions were used. Techniques employed included whole-cell patch clamp, co-immunoprecipitation, site-specific mutagenesis and Western blotting, KEY RESULTS: In VSMC, AngII induced Cl(-) currents was carried by the chloride ion channel ClC-3. This current was absent in VSMC from ClC-3 channel null mice. The AngII-induced Cl(-) current involved interactions between ClC-3 channels and Rho-kinase 2 (ROCK2), shown by N- or C-terminal truncation of ClC-3 protein, ROCK2 siRNA and co-immunoprecipitation assays. Phosphorylation of ClC-3 channels at Thr(532) by ROCK2 was critical for AngII-induced Cl(-) current and VSMC migration. The ClC-3 T532D mutant (mutation of Thr(532) to aspartate), mimicking phosphorylated ClC-3 protein, significantly potentiated AngII-induced Cl(-) current and VSMC migration, while ClC-3 T532A (mutation of Thr(532) to alanine) had the opposite effects. AngII-induced cell migration was markedly decreased in VSMC from ClC-3 channel null mice that was insensitive to Y27632, an inhibitor of ROCK2. In addition, AngII-induced cerebrovascular remodelling was decreased in ClC-3 null mice, possibly by the ROCK2 pathway. CONCLUSIONS AND IMPLICATIONS: ClC-3 protein phosphorylation at Thr(532) by ROCK2 is required for AngII-induced Cl(-) current and VSMC migration that are involved in AngII-induced vascular remodelling in hypertension.


Subject(s)
Angiotensin II/physiology , Chloride Channels/physiology , Myocytes, Smooth Muscle/physiology , Threonine/metabolism , rho-Associated Kinases/physiology , Animals , Basilar Artery/cytology , Cell Line , Cell Movement , Cells, Cultured , Chloride Channels/genetics , Male , Mice, Knockout , Muscle, Smooth, Vascular/cytology , Phosphorylation , Rats
6.
J Cardiovasc Pharmacol Ther ; 20(6): 563-71, 2015 Nov.
Article in English | MEDLINE | ID: mdl-25736282

ABSTRACT

Basilar vascular smooth muscle cells (BASMCs) hyperplasia is a prominent feature of cerebrovascular remodeling and stroke during the development of hypertension. Tanshinone IIA (Tan) has been reported to exhibit a protective effect against the pathological features of hypertension. Previous studies have shown that phosphoinostitide-3 kinase (PI3K)/3'-phosphoinostitide dependent kinase (PDK1)/AKT pathway is involved in the regulation of proliferation of various cell types. Therefore, there may be a crosstalk between Tan antihypertension processes and PI3K/PDK1/AKT proliferative effect in BASMCs. To test this hypothesis, we used a 2-kidney, 2-clip hypertension model to examine the effect of Tan on PI3K/PDK1/AKT pathway by cellular, molecular, and biochemical approaches. Our results revealed that the abundance of PDK1 in plasma was paralleled with an increase in blood pressure and the cross-sectional area of basilar artery in hypertensive rats. Tan decreased blood pressure and hypertension-induced PDK1 phosphorylation but produced no effect on the phosphorylation of PI3K. Moreover, Tan attenuated endothelin 1 induced the activation of PDK1/AKT pathway in rat BASMCs. Tan could inhibit cell cycle transition by regulating the expression of cyclin D1 and p27, in turn, prevent proliferation of BASMCs. Our study provides a novel mechanism by which Tan prevents cerebrovascular cell proliferation during hypertension, and thus Tan may be a potential therapeutic agent for cerebrovascular remodeling and stroke.


Subject(s)
Basilar Artery/cytology , Benzofurans/pharmacology , Cell Proliferation/drug effects , Hypertension/pathology , Muscle, Smooth, Vascular/drug effects , Myocytes, Smooth Muscle/drug effects , Protein Serine-Threonine Kinases/antagonists & inhibitors , Animals , Basilar Artery/pathology , Blood Pressure , Cell Cycle/drug effects , Cyclin D1/biosynthesis , Cyclin D1/genetics , Cyclin-Dependent Kinase Inhibitor p27/biosynthesis , Cyclin-Dependent Kinase Inhibitor p27/genetics , Endothelin-1/blood , Hypertension, Renovascular/pathology , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/pathology , Myocytes, Smooth Muscle/pathology , Phosphorylation , Pyruvate Dehydrogenase Acetyl-Transferring Kinase , Rats , Rats, Sprague-Dawley
7.
J Huazhong Univ Sci Technolog Med Sci ; 34(4): 482-490, 2014 Aug.
Article in English | MEDLINE | ID: mdl-25135715

ABSTRACT

Spontaneous, rhythmical contractions, or vasomotion, can be recorded from cerebral vessels under both normal physiological and pathophysiological conditions. We investigated the cellular mechanisms underlying vasomotion in the cerebral basilar artery (BA) of Wistar rats. Pressure myograph video microscopy was used to study the changes in cerebral artery vessel diameter. The main results of this study were as follows: (1) The diameters of BA and middle cerebral artery (MCA) were 314.5±15.7 µm (n=15) and 233.3±10.1 µm (n=12) at 10 mmHg working pressure (P<0.05), respectively. Pressure-induced vasomotion occurred in BA (22/28, 78.6%), but not in MCA (4/31, 12.9%) from 0 to 70 mmHg working pressure. As is typical for vasomotion, the contractile phase of the response was more rapid than the relaxation phase; (2) The frequency of vasomotion response and the diameter were gradually increased in BA from 0 to 70 mmHg working pressure. The amplitude of the rhythmic contractions was relatively constant once stable conditions were achieved. The frequency of contractions was variable and the highest value was 16.7±4.7 (n=13) per 10 min at 60 mmHg working pressure; (3) The pressure-induced vasomotion of the isolated BA was attenuated by nifedipine, NFA, 18ß-GA, TEA or in Ca(2+)-free medium. Nifedipine, NFA, 18ß-GA or Ca(2+)-free medium not only dampened vasomotion, but also kept BA in relaxation state. In contrasts, TEA kept BA in contraction state. These results suggest that the pressure-induced vasomotion of the isolated BA results from an interaction between Ca(2+)-activated Cl(-) channels (CaCCs) currents and K(Ca) currents. We hypothesize that vasomotion of BA depends on the depolarizing of the vascular smooth muscle cells (VSMCs) to activate CaCCs. Depolarization in turn activates voltage-dependent Ca(2+) channels, synchronizing contractions of adjacent cells through influx of extracellular calcium and the flow of calcium through gap junctions. Subsequent calcium-induced calcium release from ryanodine-sensitive stores activates K(Ca) channels and hyperpolarizes VSMCs, which provides a negative feedback loop for regenerating the contractile cycle.


Subject(s)
Basilar Artery/metabolism , Chloride Channels/metabolism , Muscle, Smooth, Vascular/metabolism , Myocytes, Smooth Muscle/metabolism , Potassium Channels, Calcium-Activated/metabolism , Vasoconstriction/physiology , Vasodilation/physiology , Animals , Basilar Artery/cytology , Basilar Artery/physiology , Female , Male , Membrane Potentials/physiology , Muscle, Smooth, Vascular/cytology , Myocytes, Smooth Muscle/cytology , Rats , Rats, Wistar
8.
Apoptosis ; 19(9): 1317-29, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24999019

ABSTRACT

Cystic fibrosis transmembrane conductance regulator (CFTR) acts as a cAMP-dependent chloride channel, has been studied in various types of cells. CFTR is abundantly expressed in vascular smooth muscle cells and closely linked to vascular tone regulation. However, the functional significance of CFTR in basilar vascular smooth muscle cells (BASMCs) remains elusive. Accumulating evidence has shown the direct role of CFTR in cell apoptosis that contributes to several main pathological events in CF, such as inflammation, lung injury and pancreatic insufficiency. We therefore investigated the role of CFTR in BASMC apoptotic process induced by hydrogen peroxide (H2O2). We found that H2O2-induced cell apoptosis was parallel to a significant decrease in endogenous CFTR protein expression. Silencing CFTR with adenovirus-mediated CFTR specific siRNA further enhanced H2O2-induced BASMC injury, mitochondrial cytochrome c release into cytoplasm, cleaved caspase-3 and -9 protein expression and oxidized glutathione levels; while decreased cell viability, the Bcl-2/Bax ratio, mitochondrial membrane potential, total glutathione levels, activities of superoxide dismutase and catalase. The pharmacological activation of CFTR with forskolin produced the opposite effects. These results strongly suggest that CFTR may modulate oxidative stress-related BASMC apoptosis through the cAMP- and mitochondria-dependent pathway and regulating endogenous antioxidant defense system.


Subject(s)
Apoptosis/drug effects , Basilar Artery/cytology , Cystic Fibrosis Transmembrane Conductance Regulator/metabolism , Hydrogen Peroxide/pharmacology , Myocytes, Smooth Muscle/drug effects , Animals , Basilar Artery/metabolism , Caspases/metabolism , Catalase/metabolism , Cell Survival/drug effects , Colforsin/pharmacology , Cyclic AMP/metabolism , Cystic Fibrosis Transmembrane Conductance Regulator/genetics , Cytochromes c/metabolism , Endothelial Cells/cytology , Endothelial Cells/drug effects , Endothelial Cells/metabolism , Glutathione/metabolism , Male , Membrane Potential, Mitochondrial/drug effects , Mitochondria/metabolism , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle/metabolism , Oxidative Stress/drug effects , Proto-Oncogene Proteins c-bcl-2/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Rats, Sprague-Dawley , Superoxide Dismutase/metabolism
9.
Sheng Li Xue Bao ; 66(3): 289-94, 2014 Jun 25.
Article in Chinese | MEDLINE | ID: mdl-24964845

ABSTRACT

The purposes of this study were to investigate the effect of emodin on expression of BKCa channel ß1 subunit in basilar artery smooth muscle cells (BASMCs) and electrophysiological characteristics of vascular smooth muscle cells in spontaneously hypertensive rats (SHR). Tail artery pressure measurement instrument was used to measure the change of SHR systolic blood pressure before and after emodin intervention. Single vascular smooth muscle cell was electrically recorded by whole-cell patch-clamp technique. Immunohistochemistry and Western blotting were used to study the distribution and expression of the BKCa channel ß1 subunit. The results showed that emodin decreased blood pressure of SHR from (223 ± 16) mmHg to (127 ± 12) mmHg (P < 0.01). There was no difference of blood pressure between emodin-treated SHR and Wistar rats. Emodin significantly increased outward currents of smooth muscle cells in SHR (P < 0.05), and this effect could be reversed by specific inhibitor of BKCa channel, IbTX. Emodin also up-regulated BKCa channel ß1 subunit expression in BASMCs. These results suggest that emodin relaxes cerebral basilar artery by enhancing BKCa current via increasing ß1 subunit expression in BASMCs.


Subject(s)
Emodin/pharmacology , Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/metabolism , Myocytes, Smooth Muscle/metabolism , Animals , Basilar Artery/cytology , Blood Pressure , Patch-Clamp Techniques , Rats , Rats, Inbred SHR , Rats, Wistar , Vasodilation , Vasodilator Agents/pharmacology
10.
J Neuroinflammation ; 11: 90, 2014 May 16.
Article in English | MEDLINE | ID: mdl-24886705

ABSTRACT

BACKGROUND: Cerebral ischemia induces transcriptional upregulation of inflammatory genes in the brain parenchyma and in cerebral arteries, thereby contributing to the infarct development. The present study was designed to evaluate the involvement of calcium-calmodulin-dependent protein kinase (CaMKII) II and extracellular signal-regulated kinase1/2 (ERK1/2) on inflammatory mediators in rat cerebral arteries using organ culture as a method for inducing ischemic-like vascular wall changes. METHODS: Rat basilar arteries were cultured in serum-free medium for 0, 3, 6 or 24 hours in the presence or absence of the CaMKII inhibitor KN93 or the MEK1/2 inhibitor U0126. Protein expression of activated CaMKII, ERK1/2, and inflammatory-associated protein kinases and mediators were examined with western blot and immunohistochemistry. Caspase-3 mRNA levels in basilar arteries were studied with real-time PCR. RESULTS: Western blot evaluation showed that organ culture induced a significant increase in phosphorylated ERK1/2 at 3, 6 and 24 hours, while CaMKII was found to be already activated in fresh non-incubated arteries and to decrease with incubation time. The addition of U0126 or KN93 decreased levels of phosphorylated c-Jun N-terminal kinase and p-p38, as evaluated by immunohistochemistry. KN93 affected the increase in caspase-3 mRNA expression only when given at the start of incubation, while U0126 had an inhibitory effect when given up to six hours later. Tumor necrosis factor receptor 1 was elevated after organ culture. This inflammatory marker was reduced by both of the two different protein kinase inhibitors. CONCLUSIONS: The novel findings of the present study are that the cross-talk between the two protein kinases and the inhibition of CaMKII or MEK1/2 in a time-dependent manner attenuates inflammatory-associated protein kinases and mediators, suggesting that they play a role in cerebrovascular inflammation.


Subject(s)
Basilar Artery/enzymology , Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , Culture Media, Serum-Free/pharmacology , MAP Kinase Kinase Kinase 1/metabolism , MAP Kinase Kinase Kinase 2/metabolism , Animals , Basilar Artery/cytology , Basilar Artery/drug effects , Benzylamines/pharmacology , Butadienes/pharmacology , Caspase 3/genetics , Caspase 3/metabolism , Cytokines/genetics , Cytokines/metabolism , Enzyme Inhibitors/pharmacology , Gene Expression/drug effects , Male , Muscle, Smooth/drug effects , Muscle, Smooth/metabolism , Nitriles/pharmacology , Organ Culture Techniques , Rats , Rats, Sprague-Dawley , Receptors, Tumor Necrosis Factor, Type I/genetics , Receptors, Tumor Necrosis Factor, Type I/metabolism , Signal Transduction/drug effects , Sulfonamides/pharmacology , Time Factors
11.
Naunyn Schmiedebergs Arch Pharmacol ; 387(7): 697-702, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24833299

ABSTRACT

Our previous in vitro study demonstrated that bradykinin (BK) induced relaxation and contraction of porcine basilar artery (PBA) mediated via activation of endothelial B2 receptors. The main relaxing and contracting factors appeared to be nitric oxide (NO) and prostaglandin (PG) H2, respectively, but not thromboxane A2. After obtaining these findings, we succeeded in cultivating endothelial cells isolated from the PBA. Although PGH2 has different functionally active isoforms, including PGD2, PGE2, and PGF2α, we have not yet clarified which of them is responsible for BK-induced contraction. Therefore, we attempted to quantify NO and PG production from cultured porcine basilar arterial endothelial cells (PBAECs) and to identify which of the PGs was involved in this contraction. The cultured PBAECs produced NO spontaneously, and BK enhanced this production in a concentration-dependent manner. The NO synthase inhibitor Nω-nitro-L-arginine (L-NNA) and the B2 receptor antagonist HOE-140, but not the B1 receptor antagonist des-Arg(9), [Leu(8)]-BK, completely abolished it. In a functional study, PGD2, PGE2, and PGF2α induced concentration-dependent contractions in isolated porcine basilar arterial rings, the order of maximum contraction being PGF2α > PGE2 > PGD2. The cultured PBAECs produced PGD2, PGE2, and PGF2α spontaneously, and BK significantly enhanced the production of PGF2α, but not that of PGD2 and PGE2. The B2, but not B1, antagonist completely abolished the BK-enhanced production of PGF2α. These results suggest that BK induces production of NO and PGF2α simultaneously from PBAECs via B2 receptor activation.


Subject(s)
Bradykinin/metabolism , Dinoprost/metabolism , Endothelial Cells/metabolism , Nitric Oxide/metabolism , Receptor, Bradykinin B2/metabolism , Animals , Basilar Artery/cytology , Cells, Cultured , Female , In Vitro Techniques , Isometric Contraction , Male , Swine
12.
Apoptosis ; 18(5): 556-65, 2013 May.
Article in English | MEDLINE | ID: mdl-23468120

ABSTRACT

Bestrophin 3 (Best-3) is expressed in a variety of tissues, such as cardiac, smooth muscle and renal tissues, and it is highly expressed in rat basilar arterial smooth muscle cells (BASMCs). Lee et al. (Biochim Biophys Acta 1823:1864-1876, 2012) reported that Best-3 prevented apoptotic cell death induced by endoplasmic reticulum stress. In the present study, we used small interference RNA (siRNA) and bestrophin 3 cDNA transfection strategy to investigate whether Best-3 can provide a protective effect on apoptosis induced by hydrogen peroxide (H2O2) in BASMCs and studied the underlying mechanisms. We found that silencing of Best-3 with siRNA resulted in an increased H2O2-induced apoptosis and a decreased cell viability, whereas overexpression of Best-3 significantly prevented the apoptotic cell death and increased the cell viability. Overexpression of Best-3 could stabilize the mitochondrial membrane potential, increase the ratio of Bcl-2/Bax, and decrease cytochrome c release and caspase-3 activation. In contrast, silencing of Best-3 produced the opposite effects. Our present data strongly suggest that Best-3 inhibits apoptosis induced by H2O2 in BASMCs through mitochondria dependent pathway.


Subject(s)
Basilar Artery/metabolism , Chloride Channels/genetics , Mitochondria/metabolism , Myocytes, Smooth Muscle/metabolism , Animals , Apoptosis/drug effects , Basilar Artery/cytology , Basilar Artery/drug effects , Bestrophins , Caspase 3/genetics , Caspase 3/metabolism , Cell Survival/drug effects , Chloride Channels/antagonists & inhibitors , Chloride Channels/metabolism , Cytochromes c/metabolism , Gene Expression , Gene Silencing , Hydrogen Peroxide/pharmacology , Male , Membrane Potential, Mitochondrial/drug effects , Mitochondria/drug effects , Mitochondria/genetics , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle/drug effects , Primary Cell Culture , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Rats , Rats, Sprague-Dawley , Signal Transduction , bcl-2 Homologous Antagonist-Killer Protein/genetics , bcl-2 Homologous Antagonist-Killer Protein/metabolism , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism
13.
Morfologiia ; 142(5): 39-43, 2012.
Article in Russian | MEDLINE | ID: mdl-23330436

ABSTRACT

The present study was conducted to determine the morphological and morphometric features of the arterial wall structure at the bifurcation of blood vessels of the cerebral arterial circle (CAC) of Willis in people of different age (from birth till 65 years). Material obtained from 80 people was stained with hematoxylin-eosin, Van Gieson stain, with orcein by Unna-Taenzer's method and with sudan. The proliferative activity of the cells in tunica intima and tunica media at the site of bifurcation of the internal carotid and basilar arteries was studied immunohistochemically using monoclonal antibodies against Ki-67. It was found that intimal thickenings (cushions) appeared immediately after birth, initially only in a few places of CAC vessel branching; by 8-10 years they were detected in all the bifurcations of the circle. With aging, the thickening of intimal cushions with a thinning of the underlying tunica media was found. Ki-67 protein expression was noted in both the intimal cushions and underlying tunica media, indicating the activity of atherosclerosis process.


Subject(s)
Aging , Circle of Willis/cytology , Tunica Intima/cytology , Tunica Media/cytology , Adolescent , Adult , Aged , Atherosclerosis/metabolism , Atherosclerosis/physiopathology , Basilar Artery/cytology , Basilar Artery/metabolism , Cell Proliferation , Child , Child, Preschool , Circle of Willis/metabolism , Endothelium, Vascular/cytology , Endothelium, Vascular/metabolism , Humans , Immunohistochemistry , Infant , Ki-67 Antigen/metabolism , Middle Aged , Tunica Intima/metabolism , Tunica Media/metabolism
14.
Apoptosis ; 17(2): 113-20, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22076303

ABSTRACT

Our previous studies showed that ginsenoside-Rd, a purified component from Panax notoginseng, inhibited cell proliferation and reversed basilar artery remodeling. The aim of this study was to investigate whether ginsenoside- Rd influences H(2)O(2)-induced apoptosis in basilar artery smooth muscle cells (BASMCs). The results showed that ginsenoside-Rd significantly potentiated H(2)O(2)-induced cell death and cell apoptosis. This resulted in a concentration-dependent reduction of the cell viability. Ginsenoside-Rd further increased cytochrome C release and caspase-9/caspase-3 activations, and reduced the stability of mitochondrial membrane potential (MMP) and the ratio of Bcl-2/Bax. Cyclosporine A, an inhibitor of mitochondrial-permeability transition, inhibited alteration of mitochondrial permeability induced by H(2)O(2) and reversed the effect of ginsenoside-Rd on MMP. Our data strongly suggest that ginsenoside-Rd potentiated H(2)O(2)-induced apoptosis of BASMCs through the mitochondria-dependent pathway.


Subject(s)
Apoptosis/drug effects , Gene Expression Regulation/drug effects , Ginsenosides/administration & dosage , Animals , Basilar Artery/cytology , Basilar Artery/metabolism , Cell Survival/drug effects , Dose-Response Relationship, Drug , Hydrogen Peroxide/administration & dosage , Metabolic Networks and Pathways/drug effects , Mitochondria/metabolism , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle/metabolism , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , Rats, Sprague-Dawley , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism
15.
Apoptosis ; 16(5): 468-77, 2011 May.
Article in English | MEDLINE | ID: mdl-21373935

ABSTRACT

ClC-3 Cl(-) channel plays an important role in cell volume regulation and cell cycle. In vascular smooth muscle cells, we have found that ClC-3 was involved in ET-1 induced cell proliferation. The present study was designed to further investigate the role of ClC-3 Cl(-) channel in H(2)O(2)-induced apoptosis and its underlying mechanisms in rat basilar arterial smooth muscle cell (BASMCs). By using ClC-3 cDNA and small interference RNA (siRNA) transfection strategy, it was found that overexpression of ClC-3 significantly decreased the apoptotic rate of H(2)O(2)-treated BASMCs and increased the cell viability, whereas silencing of ClC-3 with siRNA produced opposite effects and increased the apoptotic rate. ClC-3 overexpression decreased cytochrome C release and caspase-3 activation, and increased both the stability of mitochondrial membrane potential and the ratio of Bcl-2/Bax, whereas silencing of ClC-3 produced opposite effect. Furthermore, we demonstrated that overexpression of ClC-3 attenuated, whereas silencing of ClC-3 facilitated, the degradation of LaminA, one of the structural matrix proteins, in BASMCs. Our data suggest that ClC-3 Cl(-) channel can modulate H(2)O(2)-induced apoptosis in BASMCs via the intrinsic, mitochondrial pathway.


Subject(s)
Apoptosis , Basilar Artery/cytology , Chloride Channels/metabolism , Mitochondria/metabolism , Myocytes, Smooth Muscle/physiology , Animals , Apoptosis/drug effects , Basilar Artery/drug effects , Cell Survival/drug effects , Cells, Cultured , Chloride Channels/genetics , Chlorides/metabolism , Chlorides/pharmacology , Hydrogen Peroxide/pharmacology , Male , Membrane Potential, Mitochondrial/drug effects , Membrane Potential, Mitochondrial/physiology , Metabolic Networks and Pathways , Myocytes, Smooth Muscle/drug effects , Myocytes, Smooth Muscle/metabolism , Rats , Rats, Sprague-Dawley
16.
J Pharm Pharmacol ; 63(2): 253-60, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21235590

ABSTRACT

OBJECTIVES: We have previously found that volume-regulated chloride current (VRCC) is involved in cell cycle progression and cell proliferation. This study was to examine the effect of Tween 80, a nonionic surfactant, on VRCC and cell proliferation in rat basilar artery smooth muscle cells (BASMCs). METHODS: VRCC was recorded using a whole-cell patch clamp. Cell proliferation and cell cycle were determined by CCK-8, cell count and flow cytometry. KEY FINDINGS: The results showed that endothelin-1 promotes cell cycle transition from the G0/G1 phase to the S phase and significantly increases VRCC in BASMCs. The effect of Tween 80 on VRCC is reversible and concentration dependent. However, this chemical has no effect on the calcium-activated chloride channel. Tween 80 also concentration-dependently inhibits BASMCs proliferation and arrests cells in the G1/S checkpoint. The antiproliferative effect is paralleled with the inhibitory effect on VRCC. CONCLUSION: Our study demonstrates that the inhibitory effect of Tween 80 on VRCC contributes importantly to arrest of the cell cycle and prevention of cell proliferation.


Subject(s)
Chloride Channels/antagonists & inhibitors , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle/drug effects , Polysorbates/pharmacology , Animals , Basilar Artery/cytology , Basilar Artery/drug effects , Basilar Artery/metabolism , Cell Cycle/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Chloride Channels/metabolism , Chlorides/metabolism , Endothelin-1/pharmacology , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , Myocytes, Smooth Muscle/metabolism , Rats , Rats, Sprague-Dawley
17.
Kaohsiung J Med Sci ; 27(12): 538-43, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22208536

ABSTRACT

This study investigated whether KMUP-1, a xanthine-based derivative, inhibits L-type Ca(2+) currents (I(Ca,L)) in rat basilar artery smooth muscle cells (RBASMCs). We used whole cell patch-clamp recording to monitor Ba(2+) currents (I(Ba)) through L-type Ca(2+) channels (LTCCs). Under voltage-clamp conditions, holding at -40 mV, KMUP-1 (1, 3, 10 µM) inhibited I(Ba) in a concentration-dependent manner and its IC(50) value was 2.27 ± 0.45 µM. A high concentration of KMUP-1 (10 µM) showed without modifying the I(Ba) current-voltage relationship. On the other hand, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 1 µM) increase I(Ba) was inhibited by KMUP-1. Pretreatment with the PKC inhibitor chelerythrine (5 µM) intensified KMUP-1-inhibited I(Ba). However, the Rho kinase inhibitor Y-27632 (30 µM) failed to affect the I(Ba) inhibition by KMUP-1. In light of these results, we suggest that KMUP-1 inhibition of LTCCs in concentration- and voltage-dependent manners in RBASMCs may be due, at least in part, to its modulation of the PKC pathway.


Subject(s)
Basilar Artery/cytology , Calcium Channel Blockers/pharmacology , Calcium Channels, L-Type/metabolism , Myocytes, Smooth Muscle/drug effects , Piperidines/pharmacology , Protein Kinase C/metabolism , Xanthines/pharmacology , Animals , Barium Compounds/pharmacology , Benzophenanthridines/pharmacology , Chlorides/pharmacology , Female , Membrane Potentials/drug effects , Myocytes, Smooth Muscle/enzymology , Myocytes, Smooth Muscle/metabolism , Patch-Clamp Techniques , Protein Kinase C/antagonists & inhibitors , Rats , Rats, Sprague-Dawley , Signal Transduction , rho-Associated Kinases/metabolism
18.
Circ Res ; 107(8): 1002-10, 2010 Oct 15.
Article in English | MEDLINE | ID: mdl-20798361

ABSTRACT

RATIONALE: Reactive oxygen species (ROS) are implicated in the development of cardiovascular disease, and oxidants are important signaling molecules in many cell types. Recent evidence suggests that localized subcellular compartmentalization of ROS generation is an important feature of ROS signaling. However, mechanisms that transduce localized subcellular changes in redox status to functionally relevant changes in cellular processes such as Ca(2+) influx are poorly understood. OBJECTIVE: To test the hypothesis that ROS regulate L-type Ca(2+) channel activity in cerebral arterial smooth muscle. METHODS AND RESULTS: Using a total internal reflection fluorescence imaging-based approach, we found that highly localized subplasmalemmal generation of endogenous ROS preceded and colocalized with sites of enhanced L-type Ca(2+) channel sparklet activity in isolated cerebral arterial smooth muscle cells. Consistent with this observation and our hypothesis, exogenous ROS increased localized L-type Ca(2+) channel sparklet activity in isolated arterial myocytes via activation of protein kinase Cα and when applied to intact cerebral arterial segments, exogenous ROS increased arterial tone in an L-type Ca(2+) channel-dependent fashion. Furthermore, angiotensin II-dependent stimulation of local L-type Ca(2+) channel sparklet activity in isolated cells and contraction of intact arteries was abolished following inhibition of NADPH oxidase. CONCLUSIONS: Our data support a novel model of local oxidative regulation of Ca(2+) influx where vasoconstrictors coupled to NAPDH oxidase (eg, angiotensin II) induce discrete sites of ROS generation resulting in oxidative activation of adjacent protein kinase Cα molecules that in turn promote local sites of enhanced L-type Ca(2+) channel activity, resulting in increased Ca(2+) influx and contraction.


Subject(s)
Calcium Channels, L-Type/physiology , Calcium Signaling/physiology , Muscle, Smooth, Vascular/physiology , Reactive Oxygen Species/metabolism , Angiotensin II/pharmacology , Animals , Basilar Artery/cytology , Calcium Signaling/drug effects , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , NADPH Oxidases/metabolism , Patch-Clamp Techniques , Protein Kinase C-alpha/metabolism , Rats , Rats, Sprague-Dawley , Vasoconstriction/drug effects , Vasoconstriction/physiology , Vasoconstrictor Agents/pharmacology
19.
Pesqui. vet. bras ; 30(8): 685-688, ago. 2010. ilus, tab
Article in Portuguese | LILACS | ID: lil-559904

ABSTRACT

A busca pela compreensão do funcionamento do sistema nervoso resultou em um aumento de estudos morfológicos sobre a vascularização encefálica em diferentes espécies animais. As artérias encefálicas são a principal rota para transporte de sangue para o cérebro e, portanto têm um papel essencial na manutenção de atividade normal do mesmo, o que desperta, devido sua importância funcional, o interesse pela realização de vários estudos. A artéria caroticobasilar foi investigada em animais da ordem Perissodactyla, de espécies da família dos eqüídeos. Pesquisou-se a frequência da artéria caroticobasilar em 30 encéfalos de equinos adultos e mestiços, do sexo masculino. As observações realizadas mostraram que este importante vaso apresentou as seguintes frequências absolutas e percentuais: presente em 15(50 por cento) dos encéfalos examinados sendo 4 (13,3 por cento) no antímero direito, 6 (20 por cento) no antímero esquerdo e 5 (16,7 por cento) em ambos os antímeros.


The search for the functional understanding of the nervous system has resulted in an increase of morphological studies about encephalic vascularization in different animal species. The encephalic arteries are the main rote for blood transport to the brain and therefore have an essential role in the maintenance of normal brain activity. The functional importance of these vessels has led to various studies. The caroticobasilar artery was investigated in animals of order Perissodactyla, in species of Equidae family. The frequency of caroticobasilar artery was studied in 30 adult male crossbreed brain horses. The observations showed that this important vessel presented the following absolute and percentual frequencies: present in 15 (50 percent) brains 4 (13.3 percent) in the right antimere, 6 (20 percent) in the left antimere and 5 (16.7 percent) on both antimeres.


Subject(s)
Animals , Male , Adult , Basilar Artery/cytology , Cerebral Arteries/anatomy & histology , Carotid Artery, Internal , Horses/surgery , Central Nervous System/anatomy & histology , Central Nervous System/blood supply
20.
J Neurosurg ; 113(4): 870-80, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20225918

ABSTRACT

OBJECT: The L-type Ca++ channel antagonists like nimodipine have limited efficacy against vasospasm after subarachnoid hemorrhage (SAH). The authors tested the hypothesis that this is because SAH alters these channels, rendering them less responsible for contraction. METHODS: Basilar artery smooth muscle cells were isolated 4, 7, and 21 days after SAH in dogs, and Ca++ channel currents were recorded in 10-mmol/L barium. Proteins for α1 subunits of L-type Ca++ channels were measured by immunoblotting and isometric tension recordings done on rings of the basilar artery. RESULTS: High voltage-activated (HVA) Ca++ channel currents were significantly decreased and low voltage-activated (LVA) currents increased during vasospasm 4, 7, and 21 days after SAH (p < 0.05). Vasospasm was associated with a significant decrease in the number of cells with negligible LVA current while the number of cells in which the LVA current formed greater than 50% of the maximal current increased (p < 0.01). Window currents through LVA and HVA channels were significantly reduced. All changes correlated with the severity of vasospasm. There was an increase in protein for Ca(v)3.1 and Ca(v)3.3 α1 subunits that comprise T-type Ca++ channels, a decrease in L-type (Ca(v)1.2 and Ca(v)1.3) and an increase in R-type (Ca(v)2.3) Ca++ channel α1 subunits. Functionally, however, isometric tension studies showed vasospastic arteries still relaxed with nimodipine. CONCLUSIONS: Voltage-dependent Ca++ channels are altered in cerebral arteries after SAH. While decreased L-type channels may account for the lack of efficacy of nimodipine clinically, there may be other reasons such as inadequate dose, effect of nimodipine on other cellular targets, and mechanisms of vasospasm other than smooth muscle contraction mediated by activation of L-type Ca++ channels.


Subject(s)
Basilar Artery/physiopathology , Calcium Channels, L-Type/physiology , Subarachnoid Hemorrhage/physiopathology , Algorithms , Animals , Basilar Artery/cytology , Basilar Artery/drug effects , Blotting, Western , Calcium Channel Blockers/pharmacology , Calcium Channels, L-Type/drug effects , Cell Separation , Cerebral Angiography , Dogs , Electrophysiology , In Vitro Techniques , Isometric Contraction/drug effects , Isometric Contraction/physiology , Models, Neurological , Muscle Contraction/physiology , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/physiology , Patch-Clamp Techniques , Subarachnoid Hemorrhage/complications , Vasospasm, Intracranial/etiology , Vasospasm, Intracranial/physiopathology
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