Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 5 de 5
Filter
Add more filters










Database
Language
Publication year range
1.
Food Funct ; 10(12): 8286-8297, 2019 Dec 11.
Article in English | MEDLINE | ID: mdl-31723956

ABSTRACT

This study evaluated the potential impacts of supplementation with betalain-rich extracts of foods on some atherosclerotic risk factors in coronary artery disease patients. During an acute phase, 48 male patients received about 50 mg betalain/betacyanin, and their blood and urine samples were collected at 3, 8, and 24 hours after supplementations. Also, in a pilot randomized crossover trial, these participants were allocated to two-week interventions (a betacyanin-rich supplement of Opuntia stricta, a betalain-rich supplement of red beetroot and a placebo) with two-week washout periods. Then, their plasma samples were collected at the baseline after a two-week period. The concentrations of betanin in plasma and urine samples were determined using HPLC. Also, homocysteine and glucose levels, lipid profile, and blood pressure were analyzed. Additionally, quality of life and dietary intake were assessed. After these interventions, minimal amounts of betanin were found in plasma and about 0.13-0.93% in urine. Also, both supplements significantly decreased the concentration of homocysteine, glucose, total cholesterol, triglyceride, and LDL. Also, betalain-rich supplements lowered both systolic and diastolic blood pressures. Nevertheless, the clinically meaningful changes were only found in the case of Hcy, LDL, and non-HDL-c concentrations. It seems that food sources of betalains can be considered as functional foods because they improve the lipid profile and levels of homocysteine, glucose, blood pressure, and quality of life to some extent.


Subject(s)
Atherosclerosis/metabolism , Betalains/metabolism , Plant Extracts/metabolism , Atherosclerosis/blood , Atherosclerosis/physiopathology , Beta vulgaris/chemistry , Beta vulgaris/metabolism , Betalains/blood , Blood Pressure , Cholesterol/blood , Dietary Supplements/analysis , Homocysteine/blood , Humans , Lipoproteins, LDL/blood , Male , Middle Aged , Opuntia/chemistry , Opuntia/metabolism , Pilot Projects , Plant Extracts/blood , Risk Factors , Triglycerides/blood
2.
J Agric Food Chem ; 66(16): 4155-4163, 2018 Apr 25.
Article in English | MEDLINE | ID: mdl-29638119

ABSTRACT

The aim of this study was to determine profile and content of betalains in volunteers' plasma and urine after long-term exposure to fermented red beet juice. During 6 weeks, 24 healthy volunteers consumed juice with a dose of 0.7 mg betalains/kg body weight. Betalains were analyzed by means of micro-HPLC-MS/MS. Twelve betalain derivatives were found in blood plasma and urine after juice intake. The highest betalains level in blood plasma (87.65 ± 15.71 nmol/L) and urine (1.14 ± 0.12 µmol) was found after the first and second week of juice intake, respectively. During juice consumption, the contribution of betalain metabolites was higher than that of native betalains, and interindividual variability in profile and content of betalains was observed. Summarizing, it was observed that long-term and regular consumption of the juice causes stabilization of profile and content of betalains in physiological fluids of volunteers, which include native compounds and their decarboxylated and dehydrogenated metabolites.


Subject(s)
Beta vulgaris/metabolism , Betalains/blood , Betalains/urine , Fruit and Vegetable Juices/analysis , Adult , Beta vulgaris/chemistry , Female , Fermentation , Humans , Male , Plant Roots/chemistry , Plant Roots/metabolism , Volunteers
3.
Molecules ; 22(12)2017 Dec 04.
Article in English | MEDLINE | ID: mdl-29207522

ABSTRACT

The objective of this study was to develop a simple and reproducible method for the qualitative and quantitative analysis of betalains in plasma samples, based on Solid Phase Extraction (SPE) and micro-high performance liquid chromatography coupled with mass spectrometry (micro-HPLC-MS/MS). The eight betalain compounds detected and quantified were characterized in the fortified rat blood plasma samples. The developed method showed a good coefficient of determination (R² = 0.999), good recovery, precision, and appropriate limits of detection (LOD) and quantification (LOQ) for these compounds. Application of this method for the treatment of rat plasma samples collected after the betalain preparation administration, for the first time, revealed the presence of native betalains and their metabolites in plasma samples. Moreover, among them, betanin (2.14 ± 0.06 µmol/L) and isobetanin (3.28 ± 0.04 µmol/L) were found at the highest concentration. The results indicated that the combination of an SPE method with a micro-HPLC-MS/MS analysis may be successfully applied for the determination of betalains in the blood plasma.


Subject(s)
Beta vulgaris/chemistry , Betalains/blood , Chromatography, High Pressure Liquid/methods , Solid Phase Extraction/methods , Tandem Mass Spectrometry/methods , Animals , Male , Rats , Rats, Wistar
4.
Eur J Nutr ; 56(3): 1245-1254, 2017 Apr.
Article in English | MEDLINE | ID: mdl-26873098

ABSTRACT

PURPOSE: To evaluate the plasma bioavailability of betanin and nitric oxide (NOx) after consuming beetroot juice (BTJ) and whole beetroot (BF). BTJ and BF were also analysed for antioxidant capacity, polyphenol content (TPC) and betalain content. METHODS: Ten healthy males consumed either 250 ml of BTJ, 300 g of BF or a placebo drink, in a randomised, crossover design. Venous plasma samples were collected pre (baseline), 1, 2, 3, 5 and 8 h post-ingestion. Betanin content in BTJ, BF and plasma was analysed with reverse-phase high-performance liquid chromatography (HPLC) and mass spectrometry detection (LCMS). Antioxidant capacity was estimated using the Trolox equivalent antioxidant capacity (TEAC) and polyphenol content using Folin-Ciocalteu colorimetric methods [gallic acid equivalents (GAE)] and betalain content spectrophotometrically. RESULTS: TEAC was 11.4 ± 0.2 mmol/L for BTJ and 3.4 ± 0.4 µmol/g for BF. Both BTJ and BF contained a number of polyphenols (1606.9 ± 151 mg/GAE/L and 1.67 ± 0.1 mg/GAE/g, respectively), betacyanins (68.2 ± 0.4 mg/betanin equivalents/L and 19.6 ± 0.6 mg/betanin equivalents/100 g, respectively) and betaxanthins (41.7 ± 0.7 mg/indicaxanthin equivalents/L and 7.5 ± 0.2 mg/indicaxanthin equivalents/100 g, respectively). Despite high betanin contents in both BTJ (~194 mg) and BF (~66 mg), betanin could not be detected in the plasma at any time point post-ingestion. Plasma NOx was elevated above baseline for 8 h after consuming BTJ and 5 h after BF (P < 0.05). CONCLUSIONS: These data reveal that BTJ and BF are rich in phytonutrients and may provide a useful means of increasing plasma NOx bioavailability. However, betanin, the major betalain in beetroot, showed poor bioavailability in plasma.


Subject(s)
Beta vulgaris/chemistry , Betalains/pharmacokinetics , Nitrates/pharmacokinetics , Adult , Antioxidants/administration & dosage , Antioxidants/pharmacokinetics , Betacyanins/administration & dosage , Betacyanins/blood , Betacyanins/pharmacokinetics , Betalains/administration & dosage , Betalains/blood , Betaxanthins/administration & dosage , Betaxanthins/blood , Betaxanthins/pharmacokinetics , Biological Availability , Chromatography, High Pressure Liquid , Cross-Over Studies , Fruit and Vegetable Juices , Humans , Male , Nitrates/administration & dosage , Nitrates/blood , Nitric Oxide/administration & dosage , Nitric Oxide/blood , Nitric Oxide/pharmacokinetics , Plant Roots/chemistry , Polyphenols/administration & dosage , Polyphenols/blood , Polyphenols/pharmacokinetics , Pyridines/administration & dosage , Pyridines/blood , Pyridines/pharmacokinetics , Young Adult
5.
BMJ ; 331(7531): 1501, 2005 Dec 24.
Article in English | MEDLINE | ID: mdl-16373728
SELECTION OF CITATIONS
SEARCH DETAIL
...