ABSTRACT
OBJECTIVE: To study the alkaloids from Corydalis impatiens. METHODS: The alkaloids were isolated and purified by chromatography and their structures were identified by spectral data and others methods. RESULTS: Seven alkaloids were isolated and identified as bicuculline(1), ochotensine(2), ochotensimine(3), ochrobirine(4), tetrahydrothalifendine(5), norochotensimine(6), N-methylactinodaphnine(7). CONCLUSION: All these compounds are isolated from this plant for the first time.
Subject(s)
Alkaloids/isolation & purification , Corydalis/chemistry , Dioxolanes/isolation & purification , Plants, Medicinal/chemistry , Alkaloids/chemistry , Bicuculline/chemistry , Bicuculline/isolation & purification , Chromatography, Thin Layer , Dioxolanes/chemistry , Molecular Structure , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Roots/chemistryABSTRACT
pH-zone-refining counter-current chromatography was successfully applied to the separation of alkaloids from a crude extract of Corydalis decumbens (Thunb.) Pers. using a multilayer coil planet centrifuge (CPC). The experiment was performed with a two-phase solvent system composed of methyl tert-butyl ether (MtBE)-acetonitrile-water (2:2:3, v/v) where triethylamine (5-10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5-10 mM) to the aqueous mobile phase as an eluter. From 3.1 g of the crude extract, 495 mg protopine, 626 mg tetrahydropalmatine and 423 mg bicuculline were obtained each with a purity of over 93% as determined by high performance liquid chromatography. The structures of the isolated compounds were identified by electron ionization mass spectrometry (EI-MS), high-performance liquid chromatography (HPLC)-electrospray ionisation-mass spectrometry (ESI-MS) and 1H NMR.
Subject(s)
Alkaloids/isolation & purification , Corydalis/chemistry , Countercurrent Distribution/methods , Acetonitriles , Benzophenanthridines , Berberine Alkaloids/isolation & purification , Bicuculline/isolation & purification , Chromatography, High Pressure Liquid , Ethylamines , Hydrochloric Acid , Hydrogen-Ion Concentration , Methyl Ethers , Spectrometry, Mass, Electrospray IonizationABSTRACT
The isoquinoline alkaloids protopine, cryptopine, sinactine, stylopine, bicuculline, adlumine, parfumine, fumariline, fumarophycine, fumaritine, dihydrofumariline, parfumidine and dihydrosanguinarine have been determined and identified by gas chromatography-mass spectrometry in Fumaria agraria, F. bastardii, F. capreolata, F. sepium, F. densiflora, F. faurei, F. officinalis subsp. officinalis, F. parviflora, F. petteri subsp. calcarata and F. macrosepala. The chemotaxonomic significance of the results is discussed.
