[Fractional analysis of urinary alpha-amylase as a probe-free method of diagnosis of biliary dyskinesia in children]. / Fraktsionnoe opredelenie alpha-amilazy mochi kak bezzondovyi metod diagnostiki diskinezii zhelchevyvodiashchikh putei v detskom vozraste.

To diagnose biliary dyskinesia (BD) in children, a method of urine alpha-amylase measurement is offered. It is based on the presence of a direct and close correlation between amylase activity and the amount of endogenous cholecystokinin-pancreozymin in the same portion of the urine. It is concluded that fractional measurement of urine alpha-amylase can be used for diagnosing BD in childhood as a tentative test. It is advisable that it may be used for examining younger children and in cases where the employment of other methods of examination is not feasible.

Effect of bile duct obstruction on pancreatic enzyme secretion and intestinal proteolytic enzyme activity in the rat.

The role of bile in regulation of intestinal proteolytic activity in rats was investigated by studying the effects of bile diversion and bile duct obstruction on pancreatic protease secretion and on recovery of protease from the intestine. Diversion of bile and pancreatic juice from the intestine caused a large increase in pancreatic enzyme secretion; replacement of bile partially suppressed this response. Bile duct obstruction resulted (3-4 days postobstruction) in a threefold increase in pancreatic juice chymotrypsin but caused a large decreases in intestinal trypsin and chymotrypsin activities and total proteolytic activity. Recovery of pancreatic juice protein (labeled with 14C) from intestinal contents was markedly decreased in bile duct obstruction, indicating a more rapid rate of degradation and absorption of pancreatic jucie protein. The evidence suggests that interruption of bile flow results in an accelerated rate of degradation of pancreatic proteolytic enzymes, and that the increase in pancreatic enzyme secretion is an adaptation to decreased intestinal proteolytic activity.