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1.
Effective method for the heat inactivation of Blastomyces dermatitidis.
Brown, Elizabeth M; McTaggart, Lisa R; Low, Donald E; Richardson, Susan E.
Med Mycol ; 52(7): 766-9, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25049039

ABSTRACT

Manipulation of Blastomyces dermatitidis requires the use of containment level 3 (CL3) practices. However, access to CL3 laboratories is limited and working conditions are restrictive. We describe the validation of a "heat-killing" method to inactivate B. dermatitidis, thus allowing cellular material to be removed from the CL3 laboratory for subsequent DNA isolation that is suitable for genetic applications.


Subject(s)
Blastomyces/radiation effects , Disinfection/methods , Microbial Viability/radiation effects , Safety Management/methods , Animals , Blastomyces/genetics , Containment of Biohazards , Genetics, Microbial/methods , Hot Temperature , Humans , Molecular Biology/methods
2.
[Are, during radiotherapy for genital cancer in the female, side-effect influencing the existing vaginal blastomyces flora--especially the appearance of resitant mutants to be feared?]. / Sind bei der Strahlentherapie von Genitalkarzinomen der Frau Nebenwirkungen auf eine vorhandene vaginale Sprosspilzflora--insbesondere das Auftreten resistenter Mutanten--zu befürchten?
Haller, I; Mendling, W.
Mykosen ; 21(9): 313-7, 1978 Sep.
Article in German | MEDLINE | ID: mdl-360060

Subject(s)
Blastomyces/radiation effects , Genital Neoplasms, Female/radiotherapy , Mutation/radiation effects , Candida albicans/radiation effects , Female , Humans , Vagina/microbiology
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