1.
J Gen Virol
; 88(Pt 11): 3130-3132, 2007 Nov.
Article
in English
| MEDLINE
| ID: mdl-17947539
ABSTRACT
We previously showed that mouse adaptation of cDNA-derived Borna disease virus (BDV) strain He/80(FR) was associated exclusively with mutations in the viral polymerase complex. Interestingly, independent mouse adaptation of non-recombinant He/80 was correlated with different alterations in the polymerase and mutations in the viral glycoprotein. We used reverse genetics to demonstrate that changes in the polymerase which improve enzymatic activity represent the decisive host range mutations. The glycoprotein mutations did not confer replication competence in mice, although they slightly improved viral performance if combined with polymerase mutations. Our findings suggest that the viral polymerase restricts the host range of BDV.
Subject(s)
Borna disease virus/genetics , DNA-Directed RNA Polymerases/genetics , DNA-Directed RNA Polymerases/metabolism , Virus Replication , Animals , Borna Disease/mortality , Borna Disease/virology , Borna disease virus/enzymology , Borna disease virus/metabolism , Cell Line , Mice , Mutation , Viral Proteins/genetics , Viral Proteins/metabolism
2.
Vet Rec
; 135(12): 283-4, 1994 Sep 17.
Article
in English
| MEDLINE
| ID: mdl-7817508