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Food Chem ; 223: 89-95, 2017 May 15.
Article in English | MEDLINE | ID: mdl-28069129

ABSTRACT

A novel continuous microwave-assisted enzymatic digestion (cMAED) method is proposed for the digestion of protein from Scomberomorus niphonius to obtain potential antioxidant peptides. In this study, bromelain was found to have a high capacity for the digestion of the Scomberomorus niphonius protein. The following cMAED conditions were investigated: protease species, microwave power, temperature, bromelain content, acidity of the substrate solution, and incubation time. At 400W, 40°C, 1500U·g-1 bromelain, 20% substrate concentration, pH 6.0 and 5min incubation, the degree of hydrolysis and total antioxidant activity of the hydrolysates were 15.86% and 131.49µg·mL-1, respectively. The peptide analyses showed that eight of the potential antioxidant peptide sequences, which ranged from 502.32 to 1080.55Da with 4-10 amino acid residues, had features typical of well-known antioxidant proteins. Thus, the new cMAED method can be useful to obtain potential antioxidant peptides from protein sources, such as Scomberomorus niphonius.


Subject(s)
Antioxidants/analysis , Bromelains/analysis , Fish Proteins/analysis , Microwaves , Peptide Fragments/analysis , Animals , Antioxidants/metabolism , Antioxidants/radiation effects , Bromelains/metabolism , Bromelains/radiation effects , Fish Proteins/metabolism , Fishes , Hydrolysis/radiation effects , Oxidation-Reduction/radiation effects , Peptide Fragments/metabolism , Peptide Fragments/radiation effects
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