ABSTRACT
An aerobic, Gram-negative, non-pigmented, non-motile bacterium, KMM 3858(T), was isolated from a sea-ice sample collected from Peter the Great Bay of the Sea of Japan, Russia, and subjected to a phenotypic and phylogenetic study. Comparative analyses based on the 16S rRNA and recA gene sequences placed strain KMM 3858(T) within the genus Pseudochrobactrum. The major chemotaxonomic characteristics were found to be the presence of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminolipid and phosphatidylcholine, major fatty acids C(18 : 1)omega7c and C(19 : 0) cyclo, and ubiquinone Q-10, confirming the affiliation of strain KMM 3858(T) to the genus Pseudochrobactrum. On the basis of the phylogenetic analysis and the physiological and biochemical characterization, strain KMM 3858(T) should be classified as representing a novel species of the genus Pseudochrobactrum, for which the name Pseudochrobactrum glaciei sp. nov. is proposed. The type strain is strain Pi26(T) (=KMM 3858(T)=NRIC 0733(T)=JCM 15115(T)).
Subject(s)
Brucellaceae/classification , Brucellaceae/genetics , Ice Cover/microbiology , Water Microbiology , Bacterial Proteins/genetics , Brucellaceae/chemistry , Brucellaceae/isolation & purification , DNA, Bacterial/genetics , Fatty Acids/chemistry , Genes, Bacterial , Genes, rRNA , Glycerophospholipids/chemistry , Molecular Sequence Data , Phenotype , Phylogeny , RNA, Ribosomal, 16S/genetics , Rec A Recombinases/genetics , Russia , Seawater/microbiology , Sequence Analysis, DNAABSTRACT
A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K107(T), was isolated from sludge collected from a textile dye works in Korea and its taxonomic position was investigated by means of a polyphasic analysis. Strain K107(T) contained Q-10 as the predominant ubiquinone. The major fatty acids (>10% of total fatty acids) were C(18:1)omega7c and C(18:1) 2-OH. The DNA G+C content was 57.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain K107(T) was closely related to the genera Mycoplana, Brucella and Ochrobactrum. Strain K107(T) exhibited 16S rRNA gene sequence similarity values of 96.3-97.1% with respect to the type strains of two Mycoplana species and 94.8-96.8% with respect to members of the genera Brucella and Ochrobactrum. A phylogenetic analysis based on recA gene sequences showed that strain K107(T) forms a distinct phylogenetic lineage within the Alphaproteobacteria. The recA gene sequence of strain K107(T) showed similarity values of 84.5% with respect to type strains of Brucella species and values of 77.6-83.1% with respect to members of the genera Pseudochrobactrum, Ochrobactrum and Mycoplana. Strain K107(T) could be differentiated from phylogenetically related genera by differences in phenotypic properties and fatty acid profiles. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain K107(T) represents a novel genus and species, for which the name Daeguia caeni gen. nov., sp. nov. is proposed. The type strain of Daeguia caeni is strain K107(T) (=KCTC 12981(T) =CCUG 54520(T)).
