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1.
Ecol Food Nutr ; 59(6): 598-614, 2020 Nov 01.
Article in English | MEDLINE | ID: mdl-32397821

ABSTRACT

Pika Pamoja (Cook Together) is an eight-session cooking curriculum for Burundian and Congolese refugee families, culturally adapted from the evidence-based iCook 4-H curriculum to address dietary acculturation barriers to and facilitators of food security. The goal of this study was to determine the feasibility and acceptability of implementing Pika Pamoja. Researchers and a multilingual community aid implemented Pika Pamoja in a pre-post pilot intervention with randomized control (n = 5)/treatment (n = 5) dyads (youth/mother). Feasibility (recruitment/retention, implementation, fidelity testing, and assessment procedures) and acceptability (process and program evaluations) measures were collected. All 10 dyads (control and treatment) were retained throughout the study. All fidelity measures were 91% or above. The final youth assessment instrument included scales for cooking skills (α = 0.93), cooking self-efficacy (α = 0.90), openness to new foods (α = 0.81), and eating (α = 0.68), playing (α = 0.90), and setting healthful goals (α = 0.88) together as a family. The final adult instrument included scales for cooking, eating, and playing together (α = 0.68), kitchen proficiency (α = 0.89), and food security (α = 0.79). Participant feedback was uniformly positive. Based on these results, Pika Pamoja was feasible to implement and was accepted by the priority population. Larger scale studies to measure the effectiveness of Pika Pamoja to increase food security among refugee families are needed.


Subject(s)
Acculturation , Cooking , Curriculum , Diet, Healthy , Health Promotion/methods , Program Evaluation , Refugees , Adult , Burundi/ethnology , Child , Democratic Republic of the Congo/ethnology , Exercise , Feasibility Studies , Feeding Behavior , Female , Food Security , Health Behavior , Humans , Male , Middle Aged , Patient Acceptance of Health Care , Pediatric Obesity/prevention & control , Play and Playthings , Refugees/education , Southeastern United States
2.
Ecol Food Nutr ; 58(3): 247-264, 2019.
Article in English | MEDLINE | ID: mdl-30947542

ABSTRACT

Sub-Saharan African refugees in the US have reported food security rates seven times below the national average. Dietary acculturation issues may be a contributing factor. Criterion-specific sample (n = 18) was recruited using network then snowball sampling methods. Semi-structured interviews were facilitated with the aid of a culturally and linguistically appropriate interpreter. An iterative, two cycle coding analytic process was completed within NVivo 11 by two coders who sought inter-rater reliability. Codes were organized into hierarchical maps and coding matrices for direct content analysis, and pattern and theme detection. Saturation was achieved and validated with an additional two interviews. Participants were primarily Burundian (67% vs. 33% Congolese), married (72%), held no high school degree (72%), unemployed (56%) and reported limited English proficiency (72%). Barriers and facilitators to food security across all levels of the Socio-Ecological Model (SEM) were noted. Emerging themes included difficulty with language, cooking, and shopping; transportation; social network support; orientation services; reliance on nutrition assistance programs; limited culturally relevant food and land access; and program policy miscomprehension. The complex relationship between dietary acculturation barriers and facilitators at various SEM levels demonstrates the need for a multi-level intervention to improve food security among refugees.


Subject(s)
Acculturation , Diet, Healthy/ethnology , Food Supply , Refugees , Adolescent , Adult , Burundi/ethnology , Communication Barriers , Congo/ethnology , Female , Food Assistance , Humans , Middle Aged , Qualitative Research , Social Support , Socioeconomic Factors , United States , Young Adult
3.
PLoS One ; 13(10): e0204321, 2018.
Article in English | MEDLINE | ID: mdl-30273385

ABSTRACT

The community of Pygmies settled in Vyegwa-Gika provides an exceptional case study to test the role of trust in the evolution of altruism. The Vyegwa-Gika Pygmies were forced to migrate from rainforests to the savanna, changing quickly their environment, culture, and socio-economic situation. Despite the high level of poverty they suffer in this new settlement, we found evidence of strong altruistic attitudes toward trustees when playing an economic game. In addition, Vyegwa-Gika Pygmies keep small personal trust networks despite the fact they share frequent social interactions within the community. These results indicate the great effectiveness of personal trust in fostering altruism, even if the circumstances make it difficult to establish such kind of affective bonds. A theory of the evolution of altruism should therefore also account for the evolution of psychology of trust, as a key element in the process.


Subject(s)
Altruism , Ethnicity/psychology , Trust , Burundi/ethnology , Cooperative Behavior , Humans
4.
Pan Afr Med J ; 28: 54, 2017.
Article in English | MEDLINE | ID: mdl-29184606

ABSTRACT

INTRODUCTION: A Salmonella typhi outbreak was reported in a Burundian refugee camp in Rwanda in October 2015. Transmission persisted despite increased hygiene promotion activities and hand-washing facilities instituted to prevent and control the outbreak. A knowledge, attitude and practice (KAP) study was carried out to assess the effectiveness of ongoing typhoid fever preventive interventions. METHODS: A cross-sectional survey was conducted in Mahama Refugee Camp of Kirehe District, Rwanda from January to February 2016. Data were obtained through administration of a structured KAP questionnaire. Descriptive, bivariate and multivariate analysis was performed using STATA software. RESULTS: A total of 671 respondents comprising 264 (39.3%) males and 407 (60.7%) females were enrolled in the study. A comparison of hand washing practices before and after institution of prevention and control measures showed a 37% increase in the proportion of respondents who washed their hands before eating and after using the toilet (p < 0.001). About 52.8% of participants reported having heard about typhoid fever, however 25.9% had received health education. Only 34.6% and 38.6% of the respondents respectively knew how typhoid fever spreads and is prevented. Most respondents (98.2%) used pit latrines for disposal of feces. Long duration of stay in the camp, age over 35 years and being unemployed were statistically associated with poor hand washing practices. CONCLUSION: The findings of this study underline the need for bolstering up health education and hygiene promotion activities in Mahama and other refugee camp settings.


Subject(s)
Health Knowledge, Attitudes, Practice , Hygiene/standards , Sanitation/standards , Typhoid Fever/prevention & control , Adolescent , Adult , Burundi/ethnology , Cross-Sectional Studies , Disease Outbreaks/prevention & control , Female , Hand Disinfection/standards , Health Education/methods , Humans , Male , Middle Aged , Refugee Camps , Rwanda/epidemiology , Salmonella typhi/isolation & purification , Toilet Facilities/standards , Typhoid Fever/epidemiology , Young Adult
5.
Transcult Psychiatry ; 54(1): 66-85, 2017 02.
Article in English | MEDLINE | ID: mdl-28121243

ABSTRACT

A pilot study and two intensive studies were conducted to document the local vocabularies used by Burundians to describe mental health problems and their understandings about the causes. The pilot study-in which 14 different large groups of community members awaiting appointments at a village health clinic were engaged in open-ended discussions of the local terminology and causal beliefs about mental health problems-suggested three key syndromes: akabonge (a set of depression-like symptoms), guhahamuka (a set of trauma-related symptoms), and ibisigo (a set of psychosis-like symptoms). In Study 1 ( N = 542), individual interviews or surveys presented participants with the names of these syndromes and asked what they considered to be the symptoms and causes of them. Study 2 ( N = 143) cross-validated these terms with a different sample (also in individual interviews/surveys), by presenting the symptom clusters and asking what each would be called and about their causes. Findings of both studies validated this set of terms and yielded a rich body of data about causal beliefs. The influence of education level and gender on familiarity with these terms was also assessed. Implications for the development of mental health services and directions for future research are discussed.


Subject(s)
Depression/ethnology , Health Knowledge, Attitudes, Practice/ethnology , Mental Health/ethnology , Psychological Trauma/ethnology , Psychotic Disorders/ethnology , Terminology as Topic , Adult , Burundi/ethnology , Female , Humans , Male , Middle Aged , Pilot Projects
6.
Clin Interv Aging ; 11: 1129-39, 2016.
Article in English | MEDLINE | ID: mdl-27601889

ABSTRACT

BACKGROUND: Recent cross-cultural comparisons between Asian and Western cultures have shown that ageism arises more from the lack of availability of social and economic resources for older adults than from the culture itself. We tested this assumption by conducting a survey among people living in a least developed country compared with those living in a developed country. PARTICIPANTS AND METHODS: Twenty-seven Belgians living in Belgium, 29 Burundians living in Belgium, and 32 Burundians living in Burundi were included in this study. Their attitudes toward older adults were assessed using several self-reported measures. RESULTS: Statistical analyses confirmed that older people are more negatively perceived by Burundians living in Burundi than by Burundians and Belgians living in Belgium, whose attitudes did not differ from each other. CONCLUSION: Consistent with our hypothesis, our results suggest that the level of development of a country and more particularly the lack of government spending on older people (pension and health care systems) may contribute to their younger counterparts perceiving them more negatively.


Subject(s)
Ageism/ethnology , Aging/psychology , Attitude/ethnology , Cross-Cultural Comparison , Adult , Belgium/ethnology , Burundi/ethnology , Female , Humans , Male , Middle Aged , Self Report , Socioeconomic Factors , Young Adult
7.
Aust J Prim Health ; 20(1): 92-7, 2014.
Article in English | MEDLINE | ID: mdl-23482062

ABSTRACT

Reforms to the Australian health system aim to ensure that services are accessible, clinically and culturally appropriate, timely and affordable. During the reform consultation process there were urgent calls from stakeholders to specifically consider the health needs of the thousands of refugees who settle here each year, but little is known about what is needed from the refugee perspective. Access to health services is a basic requirement of achieving the quality use of medicines, as outlined in Australia's National Medicines Policy. This study aimed to identify the barriers to accessing primary health care services and explore medicine-related issues as experienced by refugee women in South Australia. Thirty-six women participated in focus groups with accredited and community interpreters and participants were from Sudan, Burundi, Congo, Burma, Afghanistan and Bhutan who spoke English (as a second language), Chin, Matu, Dari and Nepali. The main barrier to accessing primary health care and understanding GPs and pharmacists was not being able to speak or comprehend English. Interpreter services were used inconsistently or not at all. To implement the health reforms and achieve the quality use of medicines, refugees, support organisations, GPs, pharmacists and their staff require education, training and support.


Subject(s)
Communication Barriers , Cultural Competency , Cultural Diversity , Health Services Accessibility , Language , Primary Health Care , Afghanistan , Bhutan/ethnology , Burundi/ethnology , Congo/ethnology , Female , Focus Groups , Humans , Myanmar/ethnology , Refugees , South Australia , Sudan/ethnology
8.
BMC Nephrol ; 12: 40, 2011 Aug 24.
Article in English | MEDLINE | ID: mdl-21864389

ABSTRACT

BACKGROUND: Since little is known about chronic kidney disease (CKD) among people living with HIV/AIDS (PLWHA) in Sub-Saharan Africa, the prevalence and nature of CKD were assessed in Burundi through a multicenter cross-sectional study. METHODS: Patients underwent assessments at baseline and 3 months later. Glomerular Filtration Rate (GFR) was estimated using abbreviated 4-variable Modification of Diet in Renal Diseases (MDRD) and Cockroft-Gault estimation methods. Patients were classified at month 3 into various CKD stages using the National Kidney Foundation (NKF) definition, which combines GFR and urinary abnormalities. Risk factors for presence of proteinuria (PRO) and aseptic leukocyturia (LEU) were further analyzed using multiple logistic regression. RESULTS: Median age of the patients in the study (N = 300) was 40 years, 70.3% were female and 71.7% were on highly active antiretroviral therapy. Using the MDRD method, CKD prevalence in patients was 45.7%, 30.2% of whom being classified as stage 1 according to the NKF classification, 13.5% as stage 2 and 2% as stage 3. No patient was classified as stage 4 or 5. Among CKD patients with urinary abnormality, PRO accounted for 6.1% and LEU for 18.4%. Significant associations were found between LEU and non-steroidal anti-inflammatory drug (NSAID) use, previous history of tuberculosis, low body mass index and female gender and between PRO and high viral load. CONCLUSION: Our study, using a very sensitive definition for CKD evaluation, suggests a potentially high prevalence of CKD among PLWHA in Burundi. Patients should be regularly monitored and preventative measures implemented, such as monitoring NSAID use and adjustment of drug dosages according to body weight. Urine dipsticks could be used as a screening tool to detect patients at risk of renal impairment.


Subject(s)
Acquired Immunodeficiency Syndrome/ethnology , HIV-1 , Kidney Failure, Chronic/ethnology , Acquired Immunodeficiency Syndrome/diagnosis , Adult , Burundi/ethnology , Cross-Sectional Studies , Female , HIV Infections/diagnosis , HIV Infections/ethnology , Humans , Kidney Failure, Chronic/diagnosis , Male , Middle Aged , Prevalence
9.
Int Dent J ; 61(2): 109-15, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21554280

ABSTRACT

OBJECTIVE: To analyse the demographics surrounding and the sustainability of a course in Emergency Dental Care and Health Promotion developed and taught by a team of dentists from the United States to refugee camp health-care workers in two long-term refugee camps in Western Tanzania. METHODS: Refugee camp dental patient log books from Mtabila and Nyarugusu camps Kigoma, Tanzania were analysed and demographic data collected on each patient visit from the programme inception in November 2007 until August 2009. Data collection included information relevant to 1961 patient visits. Data were entered into SPSS Statistics 17.0 using the Freq application. OUTCOMES: Patient visit data included demographics involving both the resident camp populations and the surrounding communities. The distribution of patients treated by nationality was: 58% Burundian (Mtabila), 14% Congolese (Nyarugusu), and 28% Tanzanian citizens residing near both camps. Extractions accounted for 95.5% of procedures performed. Recorded incidences of post-operative complications were 1> % of patient visits. Patient visits were steady over time and a referral system was implemented for complex cases. Health promotion sessions were held in both camps. CONCLUSION: This dental programme has been self-sustaining and is providing some access to care where none existed previously. Programmes such as this may be one solution to the access to dental care problem in long-term refugee camps.


Subject(s)
Dental Care , Health Services Accessibility , Refugees , Adolescent , Adult , Burundi/ethnology , Child , Child, Preschool , Community Health Workers/education , Congo/ethnology , Dental Care/statistics & numerical data , Dental Caries/therapy , Emergency Treatment/statistics & numerical data , Health Promotion , Health Services Accessibility/statistics & numerical data , Humans , Medical Missions , Postoperative Complications/therapy , Program Development , Program Evaluation , Referral and Consultation/statistics & numerical data , Refugees/statistics & numerical data , Tanzania , Tooth Extraction/statistics & numerical data , United States , Young Adult
10.
Fam Process ; 50(1): 27-46, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21361922

ABSTRACT

The purpose of this study was to understand the secondary migration and relocation of African refugees resettled in the United States. Secondary migration refers to moves out of state, while relocation refers to moves within state. Of 73 recently resettled refugee families from Burundi and Liberia followed for 1 year through ethnographic interviews and observations, 13 instances of secondary migration and 9 instances of relocation were identified. A family ecodevelopmental framework was applied to address: Who moved again, why, and with what consequences? How did moving again impact family risk and protective factors? How might policies, researchers, and practitioners better manage refugees moving again? Findings indicated that families undertook secondary migration principally for employment, affordable housing, family reunification, and to feel more at home. Families relocated primarily for affordable housing. Parents reported that secondary migration and relocation enhanced family stability. Youth reported disruption to both schooling and attachments with peers and community. In conclusion, secondary migration and relocation were family efforts to enhance family and community protective resources and to mitigate shortcomings in resettlement conditions. Policymakers could provide newly resettled refugees jobs, better housing and family reunification. Practitioners could devise ways to better engage and support those families who consider moving.


Subject(s)
Emigration and Immigration , Refugees , Social Environment , Acculturation , Adolescent , Adult , Aged , Black People , Burundi/ethnology , Child , Housing , Humans , Liberia/ethnology , Longitudinal Studies , Middle Aged , Policy , Social Adjustment , Socioeconomic Factors , Time Factors , United States
11.
MMWR Morb Mortal Wkly Rep ; 57(32): 869-72, 2008 Aug 15.
Article in English | MEDLINE | ID: mdl-18701876

ABSTRACT

Recent immigrants and refugees constitute a substantial proportion of malaria cases in the United States, accounting for nearly one in 10 imported malaria cases involving persons with known resident status in 2006. This report describes three cases of Plasmodium falciparum malaria and two cases of Plasmodium ovale malaria that occurred during June 27-October 15, 2007 in King County, Washington. The infections were diagnosed in Burundian refugees who had recently arrived in the United States from two refugee camps in Tanzania. Since 2005, CDC has recommended presumptive malaria treatment with artemisinin-based combination therapy (ACT) (e.g., artemether-lumefantrine) for refugees from sub-Saharan Africa before their departure for the United States (2). Rising levels of resistance to the previous mainstays of treatment, chloroquine and sulfadoxine-pyrimethamine, prompted CDC to make this recommendation. Implementation has been delayed in some countries, including Tanzania, where predeparture administration of presumptive ACT for refugees started in July 2007. The cases in this report highlight the need for health-care providers who care for recently arrived Burundian and other refugee populations to be vigilant for malaria, even among refugees previously treated for the disease.


Subject(s)
Malaria/epidemiology , Refugees , Burundi/ethnology , Child , Child, Preschool , Female , Humans , Male , Tanzania/ethnology , Washington/epidemiology
15.
J Infect Dis ; 187 Suppl 1: S58-62, 2003 May 15.
Article in English | MEDLINE | ID: mdl-12721892

ABSTRACT

From March 2000 to May 2001, four camps in Kibondo District, Tanzania, hosting refugees from Burundi reported 1062 cases of measles, a highly infectious and potentially lethal disease. Of 1062 case-patients, 225 (21%) were <9 months old, 286 (27%) were 9 months to 5 years, 324 (31%) were 6-15 years, and 227 (21%) were >/=16 years old. No deaths were reported. Although, in accordance with Sphere Project guidelines for humanitarian emergencies, camp policy was to vaccinate all new arrivals aged 6 months to 15 years against measles, 152 (72%) of 210 newly arrived refugees in this age group were unvaccinated; 143 (94%) of the 152 had lived in the camp >/=1 month before rash onset. This investigation supports Sphere Project recommendations for wide age group vaccination and suggests that in some circumstances vaccination of refugees >15 years old may be beneficial.


Subject(s)
Disease Outbreaks , Measles Vaccine/administration & dosage , Measles/epidemiology , Refugees , Vaccination/methods , Adolescent , Burundi/ethnology , Child , Child, Preschool , Humans , Infant , Population Surveillance , Tanzania/epidemiology , Vaccination/standards
16.
JAMA ; 283(3): 397-402, 2000 Jan 19.
Article in English | MEDLINE | ID: mdl-10647805

ABSTRACT

CONTEXT: Little is known about pregnancy outcomes among the approximately 11 million refugees worldwide, 25% of whom are women of reproductive age. OBJECTIVE: To estimate incidence of and determine risk factors for poor pregnancy outcomes and to calculate the contribution of mortality from neonatal and maternal deaths to overall mortality in a refugee camp. DESIGN: Cross-sectional review of records and survey, conducted in February and March 1998. SETTING: Mtendeli refugee camp, Tanzania. PARTICIPANTS: For the overall assessment, 664 Burundi women who had a pregnancy outcome during a recent 5-month period (September 1, 1997-January 31, 1998) and their 679 infants; 538 women (81%) completed the survey. MAIN OUTCOME MEASURES: Incidence of fetal death (fetus born > or =500 g or > or =22 weeks' gestation with no signs of life), low birth weight (<2500 g), neonatal death (death <28 days of life), and maternal death (deaths during or within 42 days of pregnancy from any cause related to or aggravated by the pregnancy or its management). RESULTS: The fetal death rate was 45.6 per 1000 births, the neonatal mortality rate was 29.3 per 1000 live births, and 22.4% of all live births were low birth weight. Compared with women without poor pregnancy outcome, those with poor pregnancy outcome were more likely to report prior high socioeconomic status (adjusted odds ratio [OR], 1.6; 95% confidence interval [CI], 1.1-2.4), having a first or second pregnancy (OR, 2.2; 95% CI, 1.4-3.4), and having 3 or more episodes of malaria during pregnancy (OR, 2.0; 95% CI, 1.4-3.1). Neonatal and maternal deaths accounted for 16% of all deaths during the period studied. CONCLUSIONS: Poor pregnancy outcomes were common in this refugee setting, and neonatal and maternal deaths, 2 important components of reproductive health-related deaths, contributed substantially to overall mortality.


Subject(s)
Pregnancy Outcome , Refugees , Adult , Burundi/ethnology , Female , Fetal Death , Humans , Infant Mortality , Infant, Newborn , Maternal Mortality , Pregnancy , Pregnancy Complications/epidemiology , Refugees/statistics & numerical data , Risk Factors , Tanzania/epidemiology
17.
J Travel Med ; 6(3): 204-6, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10467157

ABSTRACT

Hepatitis A virus (HAV) circulation in a given area is closely related to socioeconomic standards. Following the improvement of living conditions, HAV seroprevalence rates in the population have decreased steadily during the last decades in many Western European countries, including Italy, thereby leading to a shift of risk of disease towards older age groups. Since the severity of the disease closely parallels age, a higher incidence of symptomatic cases in adults is now reported in Europe and the United States, being travel-related to a large extent. Intrafamilial person-to-person spread is also an important source of infection and transmission from children to parents may occur due to the lack of immunity in the general population. In the last two decades, Italy has been the destination of an increasing number of migrants from developing countries, where HAV is highly endemic. Furthermore, international adoption programmes cause pediatric populations from HAV endemic countries to increase in low endemic areas, possibly leading to secondary cases in close contacts.7 The aim of this paper is to report the epidemic HAV outbreak which occurred among the voluntary nursing staff of a pediatric Rwandan refugee community hosted in a village of the Brescia Province, in northern Italy.


Subject(s)
Disease Outbreaks/statistics & numerical data , Emigration and Immigration , Hepatitis A/etiology , Hepatitis A/transmission , Infectious Disease Transmission, Patient-to-Professional , Nursing Staff , Occupational Diseases/etiology , Orphanages , Refugees , Adult , Age Distribution , Burundi/ethnology , Child , Child, Preschool , Developing Countries , Disease Outbreaks/prevention & control , Hepatitis A/epidemiology , Humans , Infant , Infection Control/methods , Italy/epidemiology , Mass Screening/methods , Middle Aged , Occupational Diseases/epidemiology , Risk Factors , Seroepidemiologic Studies
18.
Image J Nurs Sch ; 31(1): 57-63, 1999.
Article in English | MEDLINE | ID: mdl-10081214

ABSTRACT

PURPOSE: To explore how two groups of children who grew up amid violence "make sense" of their experience. As violence has become a common element of some societies, growing numbers of children have been forced to live in dangerous environments. Although considerable research has been conducted with children who are abused or neglected, the needs of those who witness violence have been largely overlooked. DESIGN: Critical narrative and descriptive. Data were collected, 1995-1996, in Canada from a convenience sample of 16 refugee children of war and 16 children of battered women. METHODS: Participants were asked open-ended questions about the violence in their lives, their feelings about what occurred, their thoughts about the reasons for violence, and their ways of surviving and growing. Common themes were identified and validated. FINDINGS: Despite differences in the children's stories, many parallels were evident. Both groups of youngsters endured pain, suffering, and feelings of betrayal. Both used creative strategies to survive. The way the children endured growing up amid violence was mediated by social, political, and cultural constructions of violence. Because these meanings were unique, violence was experienced in several ways. CONCLUSIONS: Although the children showed remarkable strength and insight, they face many challenges. Despite a common perception that children are unable to talk about deeply troubling experiences, this research demonstrated that children not only want to discuss their experience, but also welcome the opportunity to do so.


Subject(s)
Battered Women/psychology , Child Development , Psychology, Adolescent , Psychology, Child , Refugees/psychology , Violence/psychology , Warfare , Adolescent , Bosnia and Herzegovina/ethnology , Burundi/ethnology , Canada , Child , Female , Humans , Interview, Psychological/methods , Liberia/ethnology , Male , Somalia/ethnology , Violence/ethnology
19.
J Clin Microbiol ; 37(3): 596-9, 1999 Mar.
Article in English | MEDLINE | ID: mdl-9986818

ABSTRACT

Body lice are vectors of three bacteria which cause human disease: Rickettsia prowazekii, the agent of epidemic typhus; Bartonella quintana, the agent of trench fever; and Borrelia recurrentis, the agent of relapsing fever. A recrudescence of body lice is being observed as the numbers of individuals living under social conditions which predispose individuals to infestation have increased. Because this phenomenon may lead to the reemergence of infections transmitted by body lice, we aimed to assess the occurrence and prevalence of the three agents described above in more than 600 body lice collected from infested individuals in the African countries of Congo, Zimbabwe, and Burundi, in France, in Russia, and in Peru. The presence of the three bacteria in each louse was determined by specific PCR amplification, and the identities of the organisms detected were confirmed by determination of the nucleotide base sequences of the amplification products. Using this approach, we were able to confirm the presence of R. prowazekii in lice collected from refugees in Burundi, among whom typhus was epidemic, and the presence of B. quintana in lice collected from all locations except the Congo. B. recurrentis was never found. Molecular approaches are convenient tools for the detection and identification of bacterial DNA in body lice and for the epidemiological study of louse-borne bacteria from countries where no medical and biological laboratory facilities are available.


Subject(s)
Bartonella quintana/isolation & purification , Borrelia/isolation & purification , Lice Infestations/epidemiology , Pediculus/microbiology , Population Surveillance/methods , Relapsing Fever/epidemiology , Rickettsia prowazekii/isolation & purification , Trench Fever/epidemiology , Typhus, Epidemic Louse-Borne/epidemiology , Animals , Burundi/ethnology , Congo/ethnology , France/epidemiology , Ill-Housed Persons , Humans , Insect Vectors , Lice Infestations/complications , Peru/epidemiology , Polymerase Chain Reaction , Refugees , Relapsing Fever/prevention & control , Russia/epidemiology , Trench Fever/prevention & control , Typhus, Epidemic Louse-Borne/prevention & control , Zimbabwe/ethnology
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