Regulated partitioning of fixed carbon (14C), sodium (Na+), potassium (K+) and glycine betaine determined salinity stress tolerance of gamma irradiated pigeonpea [Cajanus cajan (L.) Millsp].

Soil salinity is a major constraint that limits legume productivity. Pigeonpea is a salt sensitive crop. Seed gamma irradiation at a very low dose (2.5 Gy) is known to enhance seedling establishment, plant growth and yield of cereals and other crops. The present study conducted using two genetically diverse varieties of pigeonpea viz., Pusa-991 and Pusa-992 aimed at establishing the role of pre-sowing seed gamma irradiation at 0, 0.0025, 0.005, 0.01, 0.02, 0.05 and 0.1 kGy on plant growth, seed yield and seed quality under salt stress at 0, 80 and 100 mM NaCl (soil solution EC equivalent 1.92, 5.86 and 8.02 dS/m, respectively) imposed right from the beginning of the experiment. Changes in carbon flow dynamics between shoot and root and concentration of osmolyte, glycine betaine, plant uptake and shoot and root partitioning of Na+ and K+ and activity of protein degrading enzyme protease were measured under the combined effect of gamma irradiation and salt stress. Positive affect of pre-sowing exposure of seed to low dose of gamma irradiation (<0.01 kGy) under salt stress was evident in pigeonpea. Pigeonpea variety, Pusa-992 showed a better salt tolerance response than Pusa-991 and that the radiated plants performed better than the unirradiated plants even at increasing salinity level. Seed yield and seed protein and iron content were also positively affected by the low dose gamma irradiation under NaCl stress. Multiple factors interacted to determine physiological salt tolerance response of pigeonpea varieties. Gamma irradiation caused a favourable alteration in the source-sink (shoot-root) partitioning of recently fixed carbon (14C) under salt stress in pigeonpea. Gamma irradiation of seeds prior to sowing enhanced glycine betaine content and reduced protease activity at 60-day stage under various salt stress regimes. Lower partitioning of Na+and relatively higher accumulation of K+ under irradiation treatment was the other important determinants that differentiated between salt-tolerant and salt-susceptible variety of pigeonpea. The study provides evidence and physiological basis for exploring exploitation of pre-sowing exposure of seeds with low-dose gamma ray for enhancing the salt tolerance response of crop plants.

UV-induced changes of active components and antioxidant activity in postharvest pigeon pea [Cajanus cajan (L.) Millsp.] leaves.

In this study, the effect of UV irradiation (UV-A, UV-B, and UV-C) on phytochemicals, total phenolics, and antioxidant activity of postharvest pigeon pea leaves was evaluated. The response of pigeon pea leaves to UV irradiation was phytochemical specific. UV-B and UV-C induced higher levels of phytochemicals, total phenolics, and antioxidant activity in pigeon pea leaves compared with UV-A. Furthermore, UV-B irradiation proved to possess a long-lasting effect on the levels of phenolics and antioxidant activity. After adapting for 48 h at 4 °C following 4 h UV-B irradiation, total phenolics and antioxidant activity were approximately 1.5-fold and 2.2-fold increased from 39.4 mg GAE/g DM and 15.0 µmol GAE/g DM to 59.1 mg GAE/g DM and 32.5 µmol GAE/g DM, respectively. These results indicate that UV irradiation of pigeon pea leaves can be beneficial in terms of increasing active components and antioxidant activity.