New species and new records of camallanid nematodes (Nematoda, Camallanidae) from marine fishes and sea snakes in New Caledonia.

Recent examinations of camallanid nematodes (Camallanidae) from marine fishes off New Caledonia, collected in the years 2003-2011, revealed the presence of the following five new species of Procamallanus Baylis, 1923, all belonging to the subgenus Spirocamallanus Olsen, 1952: Procamallanus (Spirocamallanus) dispar n. sp. from the common ponyfish Leiognathus equulus (type host) and the striped ponyfish Aurigequula fasciata (both Leiognathidae, Perciformes); Procamallanus (Spirocamallanus) bothi n. sp. from the leopard flounder Bothus pantherinus (Bothidae, Pleuronectiformes); Procamallanus (Spirocamallanus) hexophtalmatis n. sp. from the speckled sandperch Parapercis hexophtalma (Pinguipedidae, Perciformes); Procamallanus (Spirocamallanus) synodi n. sp. from the sand lizardfish Synodus dermatogenys (Synodontidae, Aulopiformes); and Procamallanus (Spirocamallanus) thalassomatis n. sp. from the yellow-brown wrasse Thalassoma lutescens (Labridae, Perciformes). These are described based on light and scanning electron microscopical (SEM) studies. An additional three congeneric nematodes unidentifiable to species are reported from perciform fishes and a shark: Procamallanus (Spirocamallanus) sp. 3 of Moravec et al., 2006, Procamallanus (Spirocamallanus) sp. 1, and Procamallanus (Spirocamallanus) sp. 2. Ten fish species are recorded as new hosts for Camallanus carangis Olsen, 1954. Two camallanids, Procamallanus (Spirocamallanus) sp. 3 (subgravid female) and Camallanus carangis (fourth-stage larva) were also found in the digestive tract of the New Caledonian sea krait Laticauda saintgironsi, serving apparently as postcyclic and paratenic hosts, respectively, for these fish nematodes.

TITLE: Nouvelles espèces et nouveaux signalements de nématodes camallanidés (Nematoda, Camallanidae) provenant de poissons marins et de serpents de mer en Nouvelle-Calédonie. ABSTRACT: L'étude récente de nématodes camallanidés (Camallanidae) de poissons marins capturés au large de la Nouvelle-Calédonie, recueillis de 2003 à 2011, a révélé la présence des cinq nouvelles espèces suivantes de Procamallanus Baylis, 1923, toutes appartenant au sous-genre Spirocamallanus Olsen, 1952 : Procamallanus (Spirocamallanus) dispar n. sp. de Leiognathus equulus (hôte-type) et de Aurigequula fasciata (tous deux Leiognathidae, Perciformes) ; Procamallanus (Spirocamallanus) bothi n. sp. de Bothus pantherinus (Bothidae, Pleuronectiformes) ; Procamallanus (Spirocamallanus) hexophtalmatis n. sp. de Parapercis hexophtalma (Pinguipedidae, Perciformes) ; Procamallanus (Spirocamallanus) synodi n. sp. de Synodus dermatogenys (Synodontidae, Aulopiformes) ; et Procamallanus (Spirocamallanus) thalassomatis n. sp. de Thalassoma lutescens (Labridae, Perciformes). Ces espèces sont décrites sur la base d'études réalisées au microscope électronique à balayage et au microscope photonique. Trois autres nématodes congénères non identifiables au niveau spécifique sont signalés chez des poissons perciformes et un requin : Procamallanus (Spirocamallanus) sp. 3 de Moravec et al., 2006, Procamallanus (Spirocamallanus) sp. 1 et Procamallanus (Spirocamallanus) sp. 2. Dix espèces de poissons sont rapportées comme nouveaux hôtes pour Camallanus carangis Olsen, 1954. Deux camallanidés, Procamallanus (Spirocamallanus) sp. 3 (femelles subgravides) et Camallanus carangis (larve du quatrième stade) ont également été trouvés dans le tube digestif du serpent marin de Nouvelle-Calédonie, Laticauda saintgironsi, qui semble servir respectivement d'hôte post-cyclique et d'hôte paraténique pour ces nématodes de poissons.

New information on morphology and molecular data of camallanid nematodes parasitising Xenopus laevis (Anura: Pipidae) in South Africa.

Three species of nematodes from the Camallanidae that are known to infect Xenopus laevis Daudin (Anura: Pipidae) were collected from several localities across South Africa. New data on morphology, partial 28S and cox1 genes, infection levels and distribution are presented herein. The most common species, Batrachocamallanus slomei Southwell et Kirshner, 1937, from the stomach and less often oesophagus, was found in eight localities. Camallanus kaapstaadi Southwell et Kirshner, 1937, also from the oesophagus, was found in two localities and C. xenopodis Jackson et Tinsley, 1995, from the intestine, at a single locality. New localities for both C. kaapstaadi and C. xenopodis provide a geographical range extension. Males of C. xenopodis are described for the first time herein. The existence of a left spicule in the males of both the species of Camallanus Railliet and Henry, 1915 is confirmed and measurements are provided. Although C. xenopodis is distinguished from C. mazabukae Kung, 1948 in the present study, we suggest greater sampling effort in other African amphibians to confirm the species status of the latter taxon. Finally, the new molecular data showed distant relationships between collected species of Camallanus and species parasitising fish and freshwater turtles.

A new species of camallanidae from Ageneiosus ucayalensis (Pisces: Siluriformes) from Pará State, Brazil.

Procamallanus (Spirocamallanus) belenensis n. sp. is described using light and scanning electron microcopy, based on specimens taken from Ageneiosus ucayalensis, Castelnau, 1855 (Pisces: Siluriforme), a freshwater fish commonly called mandubé, in Pard State, Brazil; Procamallanus (Spirocamallanus) rarus is recorded from the same host. To our knowledge there are no previous reports of nematodes from this fish species. Procamallanus (S.) belenensis is characterized by the presence of 8-13 cuticular spiral thickenings coating 2/3 of posterior region of the buccal capsule of males and females (the anterior third is smooth); small sub-equal spicules; 3 and 4-5 pairs of pre- and post-cloacal papillae, respectively; the proportion of muscular and glandular esophagus is 1:1.5-2; males present 2 spike-like projections at the tail tip, which are absent in females by light microscopy. The most closely related species are Procamallanus (Spirocamallanus) inopinatus and Procamallanus (Spirocamallanus) pintoi. The new species differs from the most closely related species by the presence of 8-10 cuticular spiral thickenings in the buccal capsule of males and 9-13 in the females, 8 pairs of male clocal papillae (3 pre- and 5 post-cloacal, respectively), and only males bearing 2 minute, spike-like projections at the posterior end.

Genetic variation of fish parasite populations in historically connected habitats: undetected habitat fragmentation effect on populations of the nematode Procamallanus fulvidraconis in the catfish Pelteobagrus fulvidraco.

Habitat fragmentation may have some significant effects on population genetic structure because geographic distance and physical barriers may impede gene flow between populations. In this study, we investigated whether recent habitat fragmentation affected genetic structure and diversity of populations of the nematode Procamallanus fulvidraconis in the yellowhead catfish, Pelteobagrus fulvidraco. The nematode was collected from 12 localities in 7 floodplain lakes of the Yangtze River. Using 11 intersimple sequence repeat markers, analysis of molecular variance showed that genetic diversity occurred mainly within populations (70.26%). Expected heterozygosity (He) of P. fulvidraconis was barely different between connected (0.2105) and unconnected lakes (0.2083). Population subdivision (Fst) between connected lakes (0.2177) was higher than in unconnected lakes (0.1676). However, the connected and unconnected lakes did not cluster into 2 clades. A Mantel test revealed significant positive correlation between genetic and geographic distances (R = 0.5335, P < 0.01). These results suggest that habitat fragmentation did not cause genetic differentiation among populations or a reduction of diversity in isolated populations of P. fulvidraconis. At least 2 factors may increase the dispersal range of the nematode, i.e., flash flooding in summer and other species of fish that may serve as the definitive hosts. Moreover, lake fragmentation is probably a recent process; population size of the nematode in these lakes is large enough to maintain population structure.

Molecular characteristics of Camallanus Spp. (Spirurida: Camallanidae) in fishes from China based on its rDNA sequences.

In the paper, we explored the intra- and interspecific evolutionary variation among species of Camallanus collected from different fish species in various regions of China. We determined the internal transcribed spacers of ribosomal DNA (ITS rDNA) sequences of these nematodes. The divergence (uncorrected p-distance) of ITS1, ITS2, and ITS rDNA data sets confirmed 2 valid species of Camallanus in China, i.e., C. cotti and C. hypophthalmichthys. The 2 species were distinguished not only by their different morphologies and host ranges but also by a tetranucleotide microsatellite (TTGC)n present in the ITS1 region of C. cotti. Phylogenetic analyses of the nematodes disclosed 2 main clades, corresponding to different individuals of C. cotti and C. hypophthalmichthys from different fish species in various geographical locations, although the interior nodes of each clade received poor support.

Camallanus maculatus n. sp. (Nematoda: Camallanidae) in an ornamental fish Xiphophorus maculatus (Osteichthyes: Poeciliidae) cultivated in São Paulo State, Brazil

The present study describes Camallanus maculatus n. sp. (Nematoda: Camallanidae) from Xiphophorus maculatus (Osteichthyes: Poecilidae) collected in an ornamental fish farm in São Paulo State, Brazil. Thirty-three out of 40 examined fish were parasitized (82 percent prevalence) with mean intensity of 2.8 (1-20). Male specimens were 3,500µm total length and females 7,100µm total length, provided with orange-brown buccal capsule and two developed tridents. Males showed two unequal spicules, gubernaculum absent and females with vulva situated in the middle of the body. The main characteristics were the sleekly longitudinal ridges in the buccal capsule in both sexes, enlargement at the anterior end of the smaller spicule, the number of genital papillae, longer muscular and glandular portion of esophagus and round tail in female

Descreve-se Camallanus maculatus n. sp. (Nematoda: Camallanidae) em Xiphophorus maculatus (Osteichthyes: Poecilidae) capturado em uma piscicultura ornamental no estado de São Paulo. De 40 peixes examinados, 33 estavam parasitados (prevalência de 82 por cento) cuja intensidade média foi de 2,8 (1-20). Os machos apresentaram 3.500µm e as fêmeas com 7,100µm de comprimento total, providos de uma cápsula bucal laranja-castanho e dois tridentes. Observaram-se machos com espículos desiguais, gubernáculo ausente e fêmeas com vulva situada na metade do corpo. As principais características foram as estrias longitudinais lisas em cada valva da cápsula bucal em ambos os sexos, o espículo menor com alargamento na extremidade anterior, o número de papilas genitais, as porções muscular e glandular do esôfago longas e a cauda longa e arredondada na fêmea

Procamallanus (Spirocamallanus) anguillae sp. n. (Camallanidae) and some other nematodes from the Indonesian shortfin eel Anguilla bicolor in Thailand.

A new species of parasitic nematode, Procamallanus (Spirocamallanus) anguillae sp. n. (family Camallanidae), is described based on specimens recovered from the intestine of the Indonesian eel Anguilla bicolor McClelland (type host) from southern Thailand (type locality Phuket Island). It is characterized mainly by the presence of 10-13 spiral ridges in the buccal capsule, length of spicules (366-372 microm and 198-216 microm), presence of a gubernaculum, arrangement of caudal papillae, and by the broad female tail with a digit-like projection bearing two cuticular spikes. In addition, two species of larval nematodes, Physalopteridae gen. sp. and Anisakis cf. simplex (Rudolphi 1809), were recorded from A. bicolor. All species are briefly described and illustrated.

Redescription of Camallanus hypophthalmichthys Dogel and Akhmerov, 1959 (Nematoda: Camallanidae) and its first record from fishes in China.

The nematode Camallanus hypophthalmichthys Dogel and Akhmerov, 1959 is redescribed from specimens collected from the intestine of the bighead carp Aristichthys nobilis, from Liangzihu Lake (Yangtze River basin), Hubei Province, central China. The light and scanning electron microscopical examination made it possible to study in detail the morphology of this so far little-known species and to confirm its validity. The main specific features of C. hypophthalmichthys distinguishing it from the most similar Camallanus spp. is the presence of 3 small caudal processes on the male tail tip, 13-16 longitudinal ridges on the inner surface of the valve of the buccal capsule, and the arrangement of preanal and postanal genital papillae in the male. This finding represents a new host record, the first record of this parasite in the Yangtze River basin, and the first documented record of C. hypophthalmichthys from China. Camallanus hypophthalmichthys is considered a specific intestinal parasite of fishes of the cyprinid Hypophthalmichthyinae.

Camallanus carangis Olsen, 1954 (Nematoda: Camallanidae) reported from French Polynesia and Hawai'i with a redescription of the species.

The marine fish parasite Camallanus carangis Olsen, 1954 (Nematoda: Camallanidae) is redescribed from the honeycomb grouper Epinephelus merra (Serranidae) in the Tuamotu Islands of French Polynesia and the glasseye Heteropriacanthus cruentatus (Priacanthidae) in Hawai'i, both coral reef fishes. Camallanus carangis is reported for the first time from French Polynesia and Hawai'i. Camallanus marinus Schmidt and Kuntz, 1969 is designated a synonym of C. carangis and Camallanus paracarangis Velasquez, 1980 is regarded as a species inquirenda. Additionally, the genus Oncophora may need to be redefined to accommodate Camallanus aotea Slankis and Korotaeva, 1974, which bears more resemblance to Oncophora than Camallanus.

Procamallanus diacanthum n. sp from the intestine of marine fish johnius diacanthus, India

Se describe una nueva especie de nematode de la familia camallanidae railliet et Henry, 1915 del intestino del pez de mar johnius diacanthus (lacépede) de la costa de kalingapatnam. Estos ejemplares no concuerdan con la descripción de las especies del género descritas hasta ahora. Por lo tanto, una descripción detallada y posición taxonómica se dan en este artículo. La nueva especie se caracteriza por sus medidas corporales, estructura de los engrosamientos espirales, posición del anillo nervioso y poro excretorio, ausencia de gubernaculum, número y disposición de las papilas caudales, largo de las espículas y del extremo terminal de la cola. Así, puede ser considerado una nueva especie, procamallanus diacanthum

Description of a new species of the genus camallanus Railliet et Henry 1915 from freshwater fish barbus ticto from Krishna River, Andhra Pradesh India

Se describe una nueva especie de un nematode de la familia camallanidae railliet et Henry, 1915 del ovario del pez de agua dulce barbus (Puntius) ticto (Günther). La descripción se basa en dos ejemplares machos y dos ejemplares hembras, de un gran número de huéspedes examinados. Los ejemplares no concuerdan con descripciones de especies conocidas del género camallanus, en consecuencia, una nueva especie camallanus praveeni ha sido nominada para ubicar los presentes ejemplares. La nueva especie podría ser distinguida por las medidas del cuerpo, la naturaleza de los tridentes, posición del anillo nervioso y del poro excretorio, posición y largo de las espículas, número y posición de las papilas caudales posición de la vulva y forma de extremo de la cola