Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
1.
Adv Exp Med Biol ; 1452: 97-105, 2024.
Article in English | MEDLINE | ID: mdl-38805126

ABSTRACT

Ovarian cancer is the fifth-leading cause of cancer deaths among women due to the absence of available screening methods to identify early disease. Thus, prevention and early disease detection investigations are of high priority, surrounding a critical window of opportunity to better understand important pathogenic mechanisms of disease progression. Microorganisms modulate molecular interactions in humans that can influence states of health and disease, including ovarian cancer. While the mechanisms of infectious microbial invasion that trigger the immune-inflammatory axis are well studied in cancer research, the complex interactions that promote the transition of noninfectious healthy microbes to pathobiont expansion are less understood. As traditional research has focused on the influences of infectious pathogens on ovarian cancer development and progression, the impact of noninfectious microbes has gained scientific attention. The objective of this chapter is to summarize current evidence on the role of microbiota in epithelial ovarian cancer throughout disease.


Subject(s)
Carcinoma, Ovarian Epithelial , Microbiota , Ovarian Neoplasms , Humans , Female , Ovarian Neoplasms/microbiology , Carcinoma, Ovarian Epithelial/microbiology , Carcinoma, Ovarian Epithelial/pathology , Microbiota/physiology
2.
Gene ; 811: 146083, 2022 Feb 15.
Article in English | MEDLINE | ID: mdl-34856363

ABSTRACT

Nearly three hundred thousand female patients are diagnosed with ovarian cancer in the world annually, and this number shows an increasing trend. However, characteristic symptoms caused by ovarian cancer are so few that early diagnosis remains challenging, and an effective screening method has not yet been established. Here, we conducted a case-control study in Japan to analyze the association between cervicovaginal microbiome and ovarian cancer, using 16S rRNA amplicon sequencing. Analysis of DNA extracted from cervical smear samples revealed Lactobacillus-dominant and Lactobacillus-deficient, highly-diversified bacterial communities in premenopausal and postmenopausal healthy controls, respectively, as reported for vaginal microbiota previously. We found that cervicovaginal microbiota in ovarian cancer patients, regardless of their menopausal status, were frequently a diversified community and similar to those in healthy subjects at postmenopausal ages. The diverse microbiota was associated with the major histotypes of epithelial ovarian cancer, including serous ovarian cancer and ovarian clear cell cancer. The present study implies the potential of a cervicovaginal microbiome biomarker in screening ovarian cancer in premenopausal women.


Subject(s)
Carcinoma, Ovarian Epithelial/microbiology , Cervix Uteri/microbiology , Microbiota , Ovarian Neoplasms/microbiology , Vagina/microbiology , Adult , Aged , Aged, 80 and over , Bacterial Typing Techniques/methods , Biomarkers , Case-Control Studies , DNA, Bacterial , Female , Humans , Japan , Lactobacillus/classification , Lactobacillus/genetics , Metagenome , Middle Aged , Postmenopause , Premenopause , RNA, Ribosomal, 16S , Young Adult
3.
PLoS One ; 15(1): e0227707, 2020.
Article in English | MEDLINE | ID: mdl-31917801

ABSTRACT

Epithelial ovarian cancer (OC) is the most deadly cancer of the female reproductive system. To date, there is no effective screening method for early detection of OC and current diagnostic armamentarium may include sonographic grading of the tumor and analyzing serum levels of tumor markers, Cancer Antigen 125 (CA-125) and Human epididymis protein 4 (HE4). Microorganisms (bacterial, archaeal, and fungal cells) residing in mucosal tissues including the gastrointestinal and urogenital tracts can be altered by different disease states, and these shifts in microbial dynamics may help to diagnose disease states. We hypothesized that the peritoneal microbial environment was altered in patients with OC and that inclusion of selected peritoneal microbial features with current clinical features into prediction analyses will improve detection accuracy of patients with OC. Blood and peritoneal fluid were collected from consented patients that had sonography confirmed adnexal masses and were being seen at SIU School of Medicine Simmons Cancer Institute. Blood was processed and serum HE4 and CA-125 were measured. Peritoneal fluid was collected at the time of surgery and processed for Next Generation Sequencing (NGS) using 16S V4 exon bacterial primers and bioinformatics analyses. We found that patients with OC had a unique peritoneal microbial profile compared to patients with a benign mass. Using ensemble modeling and machine learning pathways, we identified 18 microbial features that were highly specific to OC pathology. Prediction analyses confirmed that inclusion of microbial features with serum tumor marker levels and control features (patient age and BMI) improved diagnostic accuracy compared to currently used models. We conclude that OC pathogenesis alters the peritoneal microbial environment and that these unique microbial features are important for accurate diagnosis of OC. Our study warrants further analyses of the importance of microbial features in regards to oncological diagnostics and possible prognostic and interventional medicine.


Subject(s)
Ascitic Fluid/microbiology , CA-125 Antigen/blood , Carcinoma, Ovarian Epithelial/diagnosis , Membrane Proteins/blood , Microbiota/genetics , Ovarian Neoplasms/diagnosis , WAP Four-Disulfide Core Domain Protein 2/analysis , Aged , Carcinoma, Ovarian Epithelial/blood , Carcinoma, Ovarian Epithelial/microbiology , Carcinoma, Ovarian Epithelial/surgery , Cross-Sectional Studies , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Female , Humans , Hysterectomy , Laparoscopy , Machine Learning , Middle Aged , Models, Biological , Ovarian Neoplasms/blood , Ovarian Neoplasms/microbiology , Ovarian Neoplasms/surgery , Ovariectomy , Pilot Projects , Preoperative Period , Prognosis , RNA, Ribosomal, 16S/genetics
4.
Lancet Oncol ; 20(8): 1171-1182, 2019 08.
Article in English | MEDLINE | ID: mdl-31300207

ABSTRACT

BACKGROUND: Various factors-including age, family history, inflammation, reproductive factors, and tubal ligation-modulate the risk of ovarian cancer. In this study, our aim was to establish whether women with, or at risk of developing, ovarian cancer have an imbalanced cervicovaginal microbiome. METHODS: We did a case-control study in two sets of women aged 18-87 years in the Czech Republic, Germany, Italy, Norway, and the UK. The ovarian cancer set comprised women with epithelial ovarian cancer and controls (both healthy controls and those diagnosed with benign gynaecological conditions). The BRCA set comprised women with a BRCA1 mutation but without ovarian cancer and controls who were wild type for BRCA1 and BRCA2 (both healthy controls and those with benign gynaecological conditions). Cervicovaginal samples were gathered from all participants with the ThinPrep system and then underwent 16S rRNA gene sequencing. For each sample, we calculated the proportion of lactobacilli species (ie, Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, and Lactobacillus jensenii), which are essential for the generation of a protective low vaginal pH, in the cervicovaginal microbiota. We grouped samples into those in which lactobacilli accounted for at least 50% of the species present (community type L) and those in which lactobacilli accounted for less than 50% of the species present (community type O). We assessed the adjusted association between BRCA1 status and ovarian cancer status and cervicovaginal microbiota community type, using a logistic regression model with a bias reduction method. FINDINGS: Participants were recruited between Jan 2, 2016, and July 21, 2018. The ovarian cancer set (n=360) comprised 176 women with epithelial ovarian cancer, 115 healthy controls and 69 controls with benign gynaecological conditions. The BRCA set (n=220) included 109 women with BRCA1 mutations, 97 healthy controls wild type for BRCA1 and BRCA2 and 14 controls with a benign gynaecological condition wild type for BRCA1 and BRCA2. On the basis of two-dimensional density plots, receiver-operating characteristic curve analysis, and age thresholds used previously, we divided the cohort into those younger than 50 years and those aged 50 years or older. In the ovarian cancer set, women aged 50 years or older had a higher prevalence of community type O microbiota (81 [61%] of 133 ovarian cancer cases and 84 [59%] of 142 healthy controls) than those younger than 50 years (23 [53%] of 43 cases and 12 [29%] of 42 controls). In the ovarian cancer set, women younger than 50 years with ovarian cancer had a significantly higher prevalence of community type O microbiota than did age-matched controls under a logistic regression model with bias correction (odds ratio [OR] 2·80 [95% CI 1·17-6·94]; p=0·020). In the BRCA set, women with BRCA1 mutations younger than 50 years were also more likely to have community type O microbiota than age-matched controls (OR 2·79 [95% CI 1·25-6·68]; p=0·012), after adjustment for pregnancy (ever). This risk was increased further if more than one first-degree family member was affected by any cancer (OR 5·26 [95% CI 1·83-15·30]; p=0·0022). In both sets, we noted that the younger the participants, the stronger the association between community type O microbiota and ovarian cancer or BRCA1 mutation status (eg, OR for community type O for cases aged <40 years in the ovarian cancer set 7·00 [95% CI 1·27-51·44], p=0·025; OR for community type O for BRCA1 mutation carriers aged <35 years in the BRCA set 4·40 [1·14-24·36], p=0·031). INTERPRETATION: The presence of ovarian cancer, or factors known to affect risk for the disease (ie, age and BRCA1 germline mutations), were significantly associated with having a community type O cervicovaginal microbiota. Whether re-instatement of a community type L microbiome by using, for example, vaginal suppositories containing live lactobacilli, would alter the microbiomial composition higher up in the female genital tract and in the fallopian tubes (the site of origin of high-grade serous ovarian cancer), and whether such changes could translate into a reduced incidence of ovarian cancer, needs to be investigated. FUNDING: EU Horizon 2020 Research and Innovation Programme, EU Horizon 2020 European Research Council Programme, and The Eve Appeal.


Subject(s)
Carcinoma, Ovarian Epithelial/microbiology , Cervix Uteri/microbiology , Ovarian Neoplasms/microbiology , Vagina/microbiology , Adolescent , Adult , Aged , Aged, 80 and over , BRCA1 Protein/genetics , Carcinoma, Ovarian Epithelial/genetics , Case-Control Studies , Female , Genetic Predisposition to Disease , Humans , Microbiota , Middle Aged , Ovarian Neoplasms/genetics , Risk Factors , Young Adult
5.
Br J Cancer ; 120(8): 855-860, 2019 04.
Article in English | MEDLINE | ID: mdl-30894687

ABSTRACT

BACKGROUND: Sexually transmitted infections (STIs) are associated with pelvic inflammatory disease and tubal pathologies. Given the tubal origin of a proportion of ovarian cancers, STIs may be relevant in their aetiology. METHODS: Antibodies indicating past infection with Chlamydia trachomatis, Mycoplasma genitalium, herpes simplex virus type 2, and against human papillomavirus oncogenes (L1 and E6+E7 oncoproteins of types 16, 18, 45) were measured in prediagnosis plasma samples in a nested case-control study in the Nurses' Health Studies (n = 337 cases 1:1 matched to controls). Logistic regression was used to estimate multivariable-adjusted relative risks (RRs) and 95% confidence intervals [CIs] comparing women seropositive vs. seronegative among all cases (invasive and borderline), invasive (n = 257), and invasive serous ovarian cancers; n = 170), and borderline ovarian tumours (n = 80). RESULTS: C. trachomatis seropositivity was associated with higher risk of ovarian cancer overall (RR = 2.07 [1.25-3.43]); results were similar for invasive, invasive serous, and borderline tumours. We observed no associations for the other STIs. Relative to women seronegative to all infections, strongest associations were observed for seropositivity to C. trachomatis plus another STI (2.74 [1.20-6.27]; C. trachomatis alone, 1.88 [1.03-3.42]; all cases); however, the RRs were not significantly different. CONCLUSIONS: C. trachomatis infection may increase ovarian cancer risk; additional studies are required.


Subject(s)
Carcinoma, Ovarian Epithelial/epidemiology , Neoplasm Invasiveness/genetics , Oncogene Proteins/genetics , Sexually Transmitted Diseases/epidemiology , Carcinoma, Ovarian Epithelial/complications , Carcinoma, Ovarian Epithelial/microbiology , Carcinoma, Ovarian Epithelial/virology , Chlamydia trachomatis/pathogenicity , Female , Herpesvirus 2, Human/pathogenicity , Human papillomavirus 16/pathogenicity , Human papillomavirus 18/pathogenicity , Humans , Mycoplasma genitalium/pathogenicity , Neoplasm Invasiveness/pathology , Papillomaviridae/pathogenicity , Pelvic Inflammatory Disease , Risk Factors , Sexually Transmitted Diseases/complications , Sexually Transmitted Diseases/microbiology , Sexually Transmitted Diseases/virology
6.
BMC Infect Dis ; 18(1): 646, 2018 Dec 12.
Article in English | MEDLINE | ID: mdl-30541469

ABSTRACT

INTRODUCTION: Mycobacterium mucogenicum is a rare but emerging cause of infections, especially in immunocompromised patients. CASE PRESENTATION: We describe a new case of M. mucogenicum catheter-related bloodstream infection in a 34-year-old woman with ovarian cancer. M. mucogenicum was at first considered as a contaminant, and susceptibility testing was not performed. Usual susceptibility of M. mucogenicum motivated prescription of clarithromycin and moxifloxacin. Finally, our isolate was confirmed susceptible to both drugs. Clinical outcome was favorable with no relapse of infection after antibiotics discontinuation despite concomitant chemotherapy. CONCLUSION: Our case illustrates the need for a clinician-microbiologist dialogue in case of suspected M. mucogenicum infection to avoid delaying appropriate management.


Subject(s)
Bacteremia/diagnosis , Catheter-Related Infections/diagnosis , Clinical Laboratory Techniques , Mycobacterium Infections/diagnosis , Nontuberculous Mycobacteria/isolation & purification , Professional Role , Adult , Anti-Bacterial Agents/therapeutic use , Bacteremia/complications , Bacteremia/drug therapy , Bacteremia/microbiology , Carcinoma, Ovarian Epithelial/complications , Carcinoma, Ovarian Epithelial/drug therapy , Carcinoma, Ovarian Epithelial/microbiology , Catheter-Related Infections/complications , Catheter-Related Infections/drug therapy , Clinical Laboratory Techniques/standards , Diagnosis, Differential , Female , Humans , Immunocompromised Host , Microbial Sensitivity Tests , Mycobacterium Infections/complications , Mycobacterium Infections/drug therapy , Nontuberculous Mycobacteria/drug effects , Ovarian Neoplasms/complications , Ovarian Neoplasms/drug therapy , Ovarian Neoplasms/microbiology
SELECTION OF CITATIONS
SEARCH DETAIL
...