ABSTRACT
Cholangitis arising from biliary infection dominates the prognosis in Caroli's disease. To clarify the influences of bacterial infection on the biliary cystogenesis, in vivo and in vitro studies were performed using the polycystic kidney (PCK) rat as an animal model of Caroli's disease. Cholangitis became a frequent histological finding in aged PCK rats, and neovascularization around the bile ducts also increased in aged PCK rats. Immunohistochemistry revealed that expression of vascular endothelial growth factor (VEGF) was increased in PCK rat biliary epithelium. In vitro, PCK cholangiocytes overexpressed VEGF, and the supernatant of cultured PCK cholangiocytes significantly increased the proliferative activity, migration, and tube formation of cultured rat vascular endothelial cells. Stimulation with lipopolysaccharide (LPS) further induced VEGF expression in PCK cholangiocytes, which might be mediated by signaling pathways involving phosphatidylinositol 3-kinase (PI3K)-Akt and c-Jun N-terminal kinase (JNK). Both LPS and VEGF increased cell proliferative activity in PCK cholangiocytes, and siRNA against VEGF significantly reduced LPS-induced cell proliferation. Thus, LPS-induced overexpression of VEGF in the biliary epithelium may lead to hypervascularity around the bile ducts; concurrently, LPS and VEGF act as cell proliferation factors for cholangiocytes. Biliary infection may thus exacerbate biliary cystogenesis in PCK rats.
Subject(s)
Bacterial Infections/complications , Bile Ducts, Intrahepatic/cytology , Caroli Disease/microbiology , Epithelial Cells/metabolism , Polycystic Kidney Diseases/microbiology , Vascular Endothelial Growth Factor A/biosynthesis , Animals , Caroli Disease/metabolism , Caroli Disease/pathology , Cell Proliferation/drug effects , Cells, Cultured , Cholangitis/metabolism , Cholangitis/microbiology , Cholangitis/pathology , Disease Models, Animal , Endothelial Cells/metabolism , JNK Mitogen-Activated Protein Kinases/metabolism , Lipopolysaccharides/pharmacology , Liver/blood supply , Microvessels/pathology , Neovascularization, Pathologic/pathology , Phosphatidylinositol 3-Kinases/metabolism , Polycystic Kidney Diseases/metabolism , Polycystic Kidney Diseases/pathology , Rats , Receptors, Vascular Endothelial Growth Factor/metabolism , Signal Transduction , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The results of surgical treatment of 7 patients, in whom disease of Karoly type I was diagnosed, were analyzed. Ultrasonographic examination, computeric tomography (CT) and retrograde pancreatocholangiography constitute highly informative diagnostic procedures in this disease. The choose of sugical treatment method of the patients depends on volumic cystic affection of intrahepatic biliary ducts and presence of strictured portions in them.
Subject(s)
Caroli Disease/diagnosis , Caroli Disease/surgery , Cholecystectomy/methods , Choledochostomy/methods , Adult , Aged , Caroli Disease/microbiology , Cholangiography , Cholangiopancreatography, Magnetic Resonance , Female , Humans , Male , Middle Aged , Monitoring, Intraoperative , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
Long-term antibiotic treatment offers a rare opportunity to study the evolution of bacteria within the same individual. The appearance of new variants has been suggested to take place via the selection of enhanced resistance in compartments of the body in which the antibiotic concentration is low. Laboratory models of protected compartments have elegantly demonstrated their potential in selecting novel variants. However, comparable data from patients have been rare. In this study, extended antibiotic therapy in a single patient suffering from multiple infected liver cysts has provided the opportunity to observe and analyse the molecular evolution of antibiotic resistance. Each isolate has the same basic ompC gene sequence that is distinct from other Escherichia coli isolates, which suggests that they derive from the same founder population. However, the isolates differ in their auxotrophic markers, in the pI values of their dominant beta-lactamase activities and in the mutations in the promoter region of the ampC gene leading to increased expression of the AmpC enzyme. The data provide strong evidence for a single focal infection expanding via parallel pathways of evolution to give a range of antibiotic-resistant isolates. These data suggest that the infected cysts provide numerous protected environments that are the foci for the separate development of distinct variants.
