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J Microbiol Methods ; 52(3): 389-93, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12531508

ABSTRACT

Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH(4)(SO(4))(2) during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.


Subject(s)
DNA, Bacterial/isolation & purification , Polymerase Chain Reaction/methods , Soil Microbiology , Alum Compounds/metabolism , Calcium Chloride/metabolism , Cations/agonists , Chlorides , Electrophoresis, Agar Gel/methods , Ferric Compounds/metabolism , Flocculation , Magnesium Chloride/metabolism
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