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1.
Immunobiology ; 220(8): 954-63, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25747940

ABSTRACT

IL-33 targeting ST2 receptor (T1/ST2), expressed on Th2 cell surface, regulates the production of cytokines like IL-17A and IL-31. We studied the role of IL-33/ST2 axis in IL-31 and IL-17A production in patients with allergic rhinitis (AR) and with concomitant allergic asthma and rhinitis (AAR). 20 healthy control subjects (HC), 14 AR and 17 AAR subjects were recruited and blood samples collected. IL-33, soluble ST2 (sST2), IL-17A and IL-31 plasma concentrations were measured by ELISA method. T1/ST2, IL-31 and IL-17A cellular expression were studied in peripheral blood mononuclear cells (PBMC) from HC, AR and AAR (n=6 for each group) by flow-cytometry. In vitro, we also evaluated the effect of beclomethasone dipropionate (BDP) on T1/ST2, IL-31 and IL-17A expression in CD3(+)T-cells from PBMC of AAR (n=6). Plasma levels of IL-33, IL-31 and IL-17A were significantly higher and sST2 was lower in patients with AR and AAR than in HC. IL-31 and IL-17A intracellular levels significantly increased, whereas T1/ST2 expression was significantly lower, in CD3(+)T-cells from AR and AAR compared to HC. Positive correlations were observed between plasmatic components of IL-33/ST2 axis and IL-31 in both AR and AAR and IL-17A in AAR. In vitro IL-31 and IL-17A intracellular levels decreased after BDP treatment, whereas T1/ST2 expression increased in cultured CD3(+)T-cells obtained from AAR. IL-33/ST2 axis is involved in Th2/IL-31 and Th17 immune response during the progression of allergic airway disease. In vitro BDP is able to control Th2/IL-31 and Th17 immune response in PBMC from allergic patients.


Subject(s)
Asthma/immunology , Interleukin-17/metabolism , Interleukin-33/immunology , Interleukins/metabolism , Receptors, Cell Surface/metabolism , Rhinitis, Allergic, Perennial/immunology , Th17 Cells/immunology , Th2 Cells/immunology , Adult , Caveolae , Caveolins/immunology , Cell Membrane/immunology , Eosinophils/immunology , Female , Humans , Interleukin-1 Receptor-Like 1 Protein , Male , Middle Aged , Protein Isoforms/immunology
2.
Cardiovasc Res ; 86(2): 219-25, 2010 May 01.
Article in English | MEDLINE | ID: mdl-20202978

ABSTRACT

Caveolae are specialized lipid rafts that form flask-shaped invaginations of the plasma membrane. They are involved in cell signalling and transport and have been shown critically regulate vascular reactivity and blood pressure. The organization and functions of caveolae are mediated by coat proteins (caveolins) and support or adapter proteins (cavins). The caveolins, caveolin-1, -2, and -3, form the structural backbone of caveolae. These proteins are also highly integrated into caveolae function and have their own activity independent of caveolae. The cavins, cavins 1-4, are involved in regulation of caveolae and modulate the function of caveolins by promoting the membrane remodelling and trafficking of caveolin-derived structures. The relationships between these different proteins are complex and intersect with many aspects of cell function. Caveolae have also been implicated in chronic inflammatory conditions and other pathologies including atherosclerosis, inflammatory bowel disease, muscular dystrophy, and generalized dyslipidaemia. The pathogenic role of the caveolins is an emerging area, however, the roles of cavins in disease is just beginning to be explored. This review will examine the relationship between caveolins and cavins and explore the role of caveolae in inflammatory signalling mechanisms.


Subject(s)
Caveolae/immunology , Caveolins/immunology , Endothelium, Vascular/immunology , Inflammation Mediators/immunology , Inflammation/immunology , Intracellular Signaling Peptides and Proteins/immunology , Signal Transduction , Animals , Endothelium, Vascular/physiopathology , Humans , Inflammation/physiopathology
3.
Methods Mol Biol ; 400: 459-68, 2007.
Article in English | MEDLINE | ID: mdl-17951752

ABSTRACT

Lipid rafts and their related membrane vesicular structures, caveolae, are cholesterol- and sphingolipid-rich microdomains of the plasma membrane that have attracted considerable interest because of their ability to concentrate numerous signaling proteins. Efforts to define the proteins that reside in lipid rafts and caveolae as well as investigations into the functional role of these microdomains in signaling, endocytosis, and other cellular processes have led to the hypothesis that they compartmentalize or prearrange molecules involved in regulating these pathways. This chapter describes biochemical approaches for defining lipid rafts and caveolae. Included are detergent- and nondetergent-based fractionations on sucrose-density gradients that isolate buoyant lipid rafts and caveolae as well as caveolin antibody-based immunoisolation of detergent-insoluble membranes that selectively isolates caveolae and not lipid rafts. Also, a general method to disrupt lipid rafts and caveolae using beta-cyclodextrin that is useful for probing the role of these microdomains in cellular processes is described. The advantages and disadvantages of the respective approaches are discussed. Taken together, these methods are useful for defining the role of lipid rafts and caveolae in cell signaling.


Subject(s)
Caveolae/chemistry , Animals , Antibodies/chemistry , Antibodies/immunology , Caveolae/immunology , Caveolae/metabolism , Caveolins/chemistry , Caveolins/immunology , Caveolins/metabolism , Cell Line , Detergents/chemistry , Humans , Signal Transduction/physiology , Sucrose/chemistry , Ultracentrifugation , beta-Cyclodextrins/chemistry
4.
J Neuroimmunol ; 165(1-2): 11-20, 2005 Aug.
Article in English | MEDLINE | ID: mdl-15925413

ABSTRACT

The expression of caveolin-1, -2, and -3 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE) was analyzed. Western blot analysis showed that three isotypes of caveolins including caveolin-1, -2 and -3 increased significantly in the spinal cords of rats during the early stage of EAE, as compared with the levels in control animals (p<0.05); the elevated level of each caveolin persisted during the peak and recovery stage of EAE. Immunohistochemistry demonstrated that caveolin-1 and -2 were expressed constitutively in the vascular endothelial cells and ependymal cells of the normal rat spinal cord, whereas caveolin-3 was almost exclusively localized in astrocytes. In EAE lesions, the immunoreactivity of caveolin-1 was increased in the ependymal cells, some astrocytes, and some inflammatory cells of the spinal cord, while that of caveolin-2 showed an intense immunoreactivity. Caveolin-3 was expressed constitutively in some astrocytes, but not in endothelial cells; its immunoreactivity was increased in reactive astrocytes in EAE lesions. The results of the Western blot analysis largely confirmed the observations obtained with immunohistochemistry. Taking all the findings into consideration, we postulate that the expression levels of each caveolin begin to increase when EAE is initiated, possibly contributing to the modulation of signal transduction pathways in the affected cells.


Subject(s)
Caveolins/biosynthesis , Encephalomyelitis, Autoimmune, Experimental/metabolism , Spinal Cord/metabolism , Animals , Caveolin 1 , Caveolin 2 , Caveolin 3 , Caveolins/immunology , Caveolins/metabolism , Encephalomyelitis, Autoimmune, Experimental/immunology , Encephalomyelitis, Autoimmune, Experimental/pathology , Female , Immune Sera , Immunohistochemistry , Immunophenotyping , Neuroglia/immunology , Neuroglia/metabolism , Neuroglia/pathology , Neurons/chemistry , Neurons/metabolism , Neurons/pathology , Protein Isoforms/biosynthesis , Protein Isoforms/immunology , Protein Isoforms/metabolism , Rats , Rats, Inbred Lew , Receptors, Antigen, T-Cell, alpha-beta/biosynthesis , Spinal Cord/pathology , Up-Regulation
5.
Biochemistry ; 43(43): 13694-706, 2004 Nov 02.
Article in English | MEDLINE | ID: mdl-15504032

ABSTRACT

Caveolin-2 is an accessory molecule and the binding partner of caveolin-1. Previously, we showed that c-Src expression leads to the tyrosine phosphorylation of Cav-2 at position 19. To further investigate the tyrosine phosphorylation of Cav-2, we have now generated a novel phospho-specific antibody directed against phospho-Cav-2 (pY27). Here, we show that Cav-2 is phosphorylated at both tyrosines 19 and 27. We reconstituted this phosphorylation event by recombinantly coexpressing c-Src and Cav-2. We generated a series of Cav-2 constructs harboring the mutation of each tyrosine to alanine, singly or in combination, i.e., Cav-2 Y19A, Y27A, and Y19A/Y27A. Recombinant expression of these mutants in Cos-7 cells demonstrated that neither tyrosine is the unique phosphorylation site, and that double mutation of tyrosines 19 and 27 to alanine abrogates Cav-2 tyrosine phosphorylation. Immunofluorescence analysis of NIH 3T3 cells revealed that the two tyrosine-phosphorylated forms of Cav-2 exhibited some distinct properties. Phospho-Cav-2 (pY19) is concentrated at cell edges and at cell-cell contacts, whereas phospho-Cav-2 (pY27) is distributed in a dotlike pattern throughout the cell surface and cytoplasm. Further functional analysis revealed that tyrosine phosphorylation of Cav-2 has no effect on its targeting to lipid rafts, but clearly disrupts the hetero-oligomerization of Cav-2 with Cav-1. In an attempt to identify upstream mediators, we investigated Cav-2 tyrosine phosphorylation in an endogenous setting. We found that in A431 cells, EGF stimulation is sufficient to induce Cav-2 phosphorylation at tyrosines 19 and 27. However, the behavior of the two phosphorylated forms of Cav-2 diverges upon EGF stimulation. First, phospho-Cav-2 (pY19) and phospho-Cav-2 (pY27) display different localization patterns. In addition, the temporal response to EGF stimulation appears to be different. Cav-2 is phosphorylated at tyrosine 19 in a rapid and transient fashion, whereas phosphorylation at tyrosine 27 is sustained over time. Three SH2 domain-containing proteins, c-Src, Nck, and Ras-GAP, were found to associate with Cav-2 in a phosphorylation-dependent manner. However, phosphorylation at tyrosine 27 appears to be more critical than phosphorylation at tyrosine 19 for this binding to occur. Taken together, these results suggest that, in addition to the common characteristics that these two sites appear to share, phospho-Cav-2 (pY19) and phospho-Cav-2 (pY27) may each possess a set of unique functional roles.


Subject(s)
Caveolins/metabolism , Phosphotyrosine/metabolism , Adaptor Proteins, Signal Transducing , Amino Acid Substitution/genetics , Animals , Antibodies, Monoclonal/biosynthesis , Antibodies, Monoclonal/metabolism , Antibody Specificity , Binding Sites, Antibody , COS Cells , Caveolin 2 , Caveolins/genetics , Caveolins/immunology , Cell Line, Transformed , Cell Line, Tumor , Epidermal Growth Factor/pharmacology , Humans , Membrane Microdomains/metabolism , Mice , Molecular Weight , NIH 3T3 Cells , Oncogene Proteins/metabolism , Phosphorylation , Phosphotyrosine/genetics , Protein Binding , Proto-Oncogene Proteins pp60(c-src)/metabolism , Proto-Oncogene Proteins pp60(c-src)/physiology , Subcellular Fractions/metabolism , Transfection , ras GTPase-Activating Proteins/metabolism , src Homology Domains
6.
Proc Natl Acad Sci U S A ; 101(39): 14186-91, 2004 Sep 28.
Article in English | MEDLINE | ID: mdl-15353589

ABSTRACT

CD26 is a T cell costimulatory molecule with dipeptidyl peptidase IV activity in its extracellular region. We previously reported that recombinant soluble CD26 enhanced T cell proliferation induced by the recall antigen tetanus toxoid (TT). However, the mechanism involved in this enhancement is not yet elucidated. We now demonstrate that CD26 binds Caveolin-1 on antigen-presenting cells, and that residues 201-211 of CD26 along with the serine catalytic site at residue 630 contribute to binding to caveolin-1 scaffolding domain. In addition, after CD26-caveolin-1 interaction on TT-loaded monocytes, caveolin-1 is phosphorylated, which links to activate NF-kappaB, followed by up-regulation of CD86. Finally, reduced caveolin-1 expression on monocytes inhibits CD26-mediated CD86 up-regulation and abrogates CD26 effect on TT-induced T cell proliferation. Taken together, these results strongly suggest that CD26-caveolin-1 interaction plays a role in the up-regulation of CD86 on TT-loaded monocytes and subsequent engagement with CD28 on T cells, leading to antigen-specific T cell activation.


Subject(s)
Antigen-Presenting Cells/metabolism , Antigens, CD/biosynthesis , Caveolins/metabolism , Dipeptidyl Peptidase 4/physiology , Membrane Glycoproteins/biosynthesis , Up-Regulation/immunology , Animals , Antigen-Presenting Cells/immunology , B7-2 Antigen , COS Cells , Caveolin 1 , Caveolins/deficiency , Caveolins/genetics , Caveolins/immunology , Cell Line , Chlorocebus aethiops , Dipeptidyl Peptidase 4/drug effects , Humans , Immunoprecipitation , Lymphocyte Activation/immunology , Monocytes/cytology , Monocytes/immunology , Monocytes/metabolism , NF-kappa B/metabolism , Phosphorylation , RNA, Small Interfering/pharmacology , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/metabolism , T-Lymphocytes/cytology , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Tetanus Toxoid/immunology
7.
Mol Biol Rep ; 31(2): 67-84, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15293782

ABSTRACT

Caveolae appear in a multitude of processes encompassing growth regulation and trafficking. We demonstrate the abundant presence of ESA/reggie-1/flotillin-2, ATP synthase beta subunit and annexin V in endothelial caveolae by immunopurification of caveolae from vascular endothelial membrane. Five proteins are abundant in a caveolin-1 protein complex, analyzed by sucrose gradient velocity sedimentation following octyl-beta-D-glucopyranoside extraction. Caveolin-1 alpha interacts with caveolin-1beta, caveolin-2, actin, the microsomal form of NADH cytochrome B5 reductase and ESA/reggie-1/flotillin-2 as shown by co-immunoprecipitation. We propose the concept that ATP biosynthesis in caveolae regulates mechanosignaling and is induced by membrane depolarization and a proton gradient. Pressure stimuli and metabolic changes may trigger gene regulation in endothelial cells, involving a nuclear conformer of caveolin-1, shown here with an epitope-specific caveolin-1 antibody, and immediate response of ion channel activity, regulated by ESA/reggie-1/flotillin-2.


Subject(s)
Caveolae/chemistry , Membrane Proteins/analysis , Animals , Annexin A5/analysis , Annexin A5/immunology , Annexin A5/metabolism , Caveolae/immunology , Caveolae/metabolism , Caveolin 1 , Caveolin 2 , Caveolins/analysis , Caveolins/immunology , Caveolins/metabolism , Cell Line , Cytochrome-B(5) Reductase/immunology , Cytochrome-B(5) Reductase/metabolism , Endothelial Cells/immunology , Endothelial Cells/metabolism , Immunoprecipitation , Lung/cytology , Lung/immunology , Membrane Proteins/immunology , Membrane Proteins/metabolism , Mice , Nerve Tissue Proteins/analysis , Nerve Tissue Proteins/immunology , Nerve Tissue Proteins/metabolism , Proton-Translocating ATPases/analysis , Proton-Translocating ATPases/immunology , Proton-Translocating ATPases/metabolism , Rats
9.
Biochem J ; 377(Pt 3): 741-7, 2004 Feb 01.
Article in English | MEDLINE | ID: mdl-14570588

ABSTRACT

Although studies in recombinant cells indicate that scavenger receptor class B, type I (SR-BI) can promote cholesterol efflux, investigations in transgenic mice overexpressing or deficient in SR-BI endorse its physiological function as selectively sequestering cholesteryl esters from high-density lipoproteins (HDLs). Less clear is the role of SR-BII, a splice variant of the SR-B gene that differs only in the C-terminal cytoplasmic domain. Here, we identify several putative signalling motifs in the C-terminus of human SR-BII, which are absent from SR-BI, and hypothesize that these motifs interact with signalling molecules to mobilize stored cholesteryl esters and/or promote the efflux of intracellular free cholesterol. 'Pull-down' assays using a panel of tagged SH3 (Src homology 3) domains showed that cytoplasmic SR-BII, but not cytoplasmic SR-BI, bound the SH3 domain of phospholipase C-gamma1; this interaction was not, however, detected under more physiological conditions. Specific anti-peptide antisera identified SR-BII in human monocyte/macrophage THP-1 cells and, in recombinant cells, revealed receptor localization to caveolae, a plasma membrane microdomain that concentrates signal-transducer molecules and acts as a conduit for cholesterol flux between cells and lipoproteins. Consistent with its caveolar localization, expression of human SR-BII in recombinant Chinese hamster ovary cells (CHO-SR-BII) was associated with increased HDL-mediated cholesterol efflux. Nevertheless, when CHO-SR-BII cells were pre-loaded with cholesteryl [(3)H]oleate and incubated with HDL, cholesteryl ester stores were not reduced compared with control cells. We conclude that although human SR-BII is expressed by macrophages, contains cytoplasmic signalling motifs and localizes to caveolae, its ability to stimulate cholesterol efflux does not reflect enhanced hydrolysis of stored cholesteryl esters.


Subject(s)
CD36 Antigens/metabolism , Cholesterol/metabolism , Membrane Proteins , Receptors, Lipoprotein , Sialoglycoproteins , Amino Acid Sequence , Animals , CD36 Antigens/chemistry , CD36 Antigens/physiology , CHO Cells , Carcinoma, Hepatocellular/chemistry , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Caveolin 1 , Caveolins/immunology , Caveolins/metabolism , Cell Line , Cell Line, Tumor , Cholesterol Esters/metabolism , Cricetinae , Cytoplasm/chemistry , Humans , Liver Neoplasms/complications , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Lysosomal Membrane Proteins , Macrophages/chemistry , Macrophages/metabolism , Molecular Sequence Data , Monocytes/chemistry , Monocytes/metabolism , Peptides/physiology , Precipitin Tests , Protein Structure, Tertiary/physiology , Receptors, Scavenger , Scavenger Receptors, Class B , Signal Transduction/physiology
10.
J Pathol ; 200(3): 396-405, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12845636

ABSTRACT

Caveolae and their proteins, the caveolins, transport macromolecules; compartmentalize signalling molecules; and are involved in various repair processes. There is little information regarding their role in the pathogenesis of significant renal syndromes such as acute renal failure (ARF). In this study, an in vivo rat model of 30 min bilateral renal ischaemia followed by reperfusion times from 4 h to 1 week was used to map the temporal and spatial association between caveolin-1 and tubular epithelial damage (desquamation, apoptosis, necrosis). An in vitro model of ischaemic ARF was also studied, where cultured renal tubular epithelial cells or arterial endothelial cells were subjected to injury initiators modelled on ischaemia-reperfusion (hypoxia, serum deprivation, free radical damage or hypoxia-hyperoxia). Expression of caveolin proteins was investigated using immunohistochemistry, immunoelectron microscopy, and immunoblots of whole cell, membrane or cytosol protein extracts. In vivo, healthy kidney had abundant caveolin-1 in vascular endothelial cells and also some expression in membrane surfaces of distal tubular epithelium. In the kidneys of ARF animals, punctate cytoplasmic localization of caveolin-1 was identified, with high intensity expression in injured proximal tubules that were losing basement membrane adhesion or were apoptotic, 24 h to 4 days after ischaemia-reperfusion. Western immunoblots indicated a marked increase in caveolin-1 expression in the cortex where some proximal tubular injury was located. In vitro, the main treatment-induced change in both cell types was translocation of caveolin-1 from the original plasma membrane site into membrane-associated sites in the cytoplasm. Overall, expression levels did not alter for whole cell extracts and the protein remained membrane-bound, as indicated by cell fractionation analyses. Caveolin-1 was also found to localize intensely within apoptotic cells. The results are indicative of a role for caveolin-1 in ARF-induced renal injury. Whether it functions for cell repair or death remains to be elucidated.


Subject(s)
Acute Kidney Injury/metabolism , Caveolins/physiology , Acute Kidney Injury/immunology , Animals , Apoptosis/physiology , Blotting, Western/methods , Caveolin 1 , Caveolins/immunology , Cell Adhesion/immunology , Cell Membrane/immunology , Cell Membrane/metabolism , Cytosol/metabolism , Disease Models, Animal , Immunohistochemistry/methods , Ischemia/immunology , Ischemia/physiopathology , Kidney/blood supply , Male , Membrane Proteins/analysis , Membrane Proteins/immunology , Microscopy, Confocal/methods , Microscopy, Electron/methods , Rats , Rats, Sprague-Dawley
11.
Cell Tissue Res ; 311(1): 31-45, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12483282

ABSTRACT

Human alveolar type II cells were isolated from lung tissue and cultured for several days. The morphology of cells was investigated at different time points postseeding and the synthesis of alveolar cell-type specific proteins was analyzed using different methods. The rationale of the study was to characterize a primary cell culture of human alveolar cells for the development of an in vitro model studying pulmonary drug delivery. In vitro test systems based on human cells are attracting increasing interest as important alternatives to animal-derived models because possible interspecies differences in alveolar cell biology and transport mechanisms cannot be excluded. In our study, both morphological characterization and marker protein synthesis of human alveolar cells in culture indicate the differentiation of isolated alveolar type II cells into epithelial monolayers consisting of alveolar type I-like and alveolar type II-like cells, which corresponds to the composition of the alveolar epithelium of the donor tissue. By using flow cytometry, immunofluorescence, immunoblotting and reverse transcriptase polymerase chain reaction (RT-PCR), we observed a shift in the synthesis of important marker proteins. Early cultures were characterized by low caveolin-1 and high Sp-C levels. In comparison, the protein biosynthesis of alveolar cells switched with time of culture to high caveolin-1 and low Sp-C levels. Based on the similarity between human alveolar epithelium and the development of our primary alveolar cell culture, we suggest that the culture may serve as a suitable model to study epithelial transport or cell biological processes in human alveolar cells.


Subject(s)
Caveolins/genetics , Epithelial Cells/metabolism , Pulmonary Alveoli/cytology , Pulmonary Surfactant-Associated Protein C/analysis , Respiratory Mucosa/cytology , Antibodies , Biological Transport , Biomarkers , Caveolin 1 , Caveolins/analysis , Caveolins/immunology , Cell Differentiation , Cells, Cultured , Epithelial Cells/chemistry , Epithelial Cells/ultrastructure , Flow Cytometry , Humans , Microscopy, Electron, Scanning , Microscopy, Immunoelectron , Pulmonary Alveoli/metabolism , Pulmonary Surfactant-Associated Protein C/immunology , RNA, Messenger/analysis , Respiratory Mucosa/metabolism , Reverse Transcriptase Polymerase Chain Reaction
12.
Mol Cell Neurosci ; 19(1): 18-31, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11817895

ABSTRACT

It is still largely unclear how cell adhesion molecule (CAM)-mediated signaling evokes responses from the growth cone cytoskeleton. Here we used TX-114 extraction of growth cones followed by equilibrium gradient centrifugation to isolate subfractions of detergent-resistant microdomains (DRMs) that could be structurally and functionally distinguished on the basis of localization and activation of components of CAM-mediated signaling pathways. DRMs enriched in cholesterol, caveolin, NCAM140, GPI-linked NCAM120, fyn, and GAP-43, all conventional markers of microdomains or rafts, were located in areas 2 and 3 of the gradient. Coimmunoprecipitation of specific components of CAM signaling pathways by GAP-43 then identified distinct subpopulations of DRMs. GAP-43 from area 2 DRMs coprecipitated GPI-linked NCAM120 and was inactive, i.e., PKC phosphorylation had not been stimulated. In contrast the GAP-43 from area 3 DRMs coprecipitated both transmembrane NCAM140 and caveolin and was active, i.e., highly phosphorylated by PKC. A different subset of DRMs from both area 2 and area 3 contained fyn that could not be coprecipitated with GAP-43 antibodies. In this case area 2 DRMs contained activated fyn that was phosphorylated on Y415. In contrast area 3 DRMs contained inactive fyn. Hence fyn and GAP-43, both targets of NCAM signaling, are located in distinct populations of DRMs, and their activated forms are reciprocally distributed on the gradient. A detergent-resistant membrane fraction recovered from area 4 was enriched in NCAM140, phosphorylated GAP-43, and actin, but not cholesterol, caveolin, or fyn. Immunoelectron microscopy revealed that phosphorylated GAP-43 was localized where the membranes and F-actin interacted. Our results provide evidence for NCAM-mediated signaling in DRMs and suggest that the DRMs responsible for fyn and PKC/GAP-43-mediated NCAM signaling are structurally distinct and differentially distributed in growth cones.


Subject(s)
Growth Cones/physiology , Neural Cell Adhesion Molecules/chemistry , Neural Cell Adhesion Molecules/isolation & purification , Signal Transduction/physiology , Actins/isolation & purification , Animals , Antibody Specificity , Brain Chemistry/physiology , Caveolin 1 , Caveolins/immunology , Caveolins/isolation & purification , Cell Fractionation/methods , Cell Membrane/chemistry , Cell Membrane/ultrastructure , Centrifugation, Density Gradient , Cytoskeleton/chemistry , Cytoskeleton/ultrastructure , Detergents , GAP-43 Protein/immunology , GAP-43 Protein/isolation & purification , Growth Cones/chemistry , Microscopy, Immunoelectron , Neural Cell Adhesion Molecules/immunology , Octoxynol , Polyethylene Glycols , Precipitin Tests , Protein Structure, Tertiary , Rats , Sucrose
13.
FEBS Lett ; 508(1): 49-52, 2001 Nov 09.
Article in English | MEDLINE | ID: mdl-11707266

ABSTRACT

Caveolins are scaffolding proteins able to collect on caveolae a large number of signalling proteins bearing a caveolin-binding motif. The proteins of the striatin family, striatin, SG2NA, and zinedin, are composed of several conserved, collinearly aligned, protein-protein association domains, among which a putative caveolin-binding domain [Castets et al. (2000) J. Biol. Chem. 275, 19970-19977]. They are associated in part with membranes. These proteins are mainly expressed within neurons and thought to act both as scaffolds and as Ca(2+)-dependent signalling proteins [Bartoli et al. (1999) J. Neurobiol. 40, 234-243]. Here, we show that (1) rat brain striatin, SG2NA and zinedin co-immunoprecipitate with caveolin-1; (2) all are pulled down by glutathione-S-transferase (GST)-caveolin-1; (3) a fragment of recombinant striatin containing the putative caveolin-binding domain binds GST-caveolin-1. Hence, it is likely that the proteins of the striatin family are addressed to membrane microdomains by their binding to caveolin, in accordance with their putative role in membrane trafficking [Baillat et al. (2001) Mol. Biol. Cell 12, 663-673].


Subject(s)
Calmodulin-Binding Proteins/metabolism , Calmodulin/metabolism , Caveolins/metabolism , Membrane Proteins/metabolism , Nerve Tissue Proteins/metabolism , Animals , Autoantigens/metabolism , Caveolin 1 , Caveolins/immunology , Cholic Acids/chemistry , Detergents/chemistry , Precipitin Tests , Protein Binding , Rats , Rats, Wistar , Recombinant Fusion Proteins/metabolism
14.
Cell Tissue Res ; 305(1): 99-106, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11512676

ABSTRACT

To search for caveolar proteins, mice were immunised with rat adipocyte membranes. Hybridoma supernatants were screened for antibodies to proteins on the cytosolic face of caveolae by indirect immunoelectron microscopy of immunogold-labelled adipocyte plasma membrane sheets adsorbed on electron-microscope (EM) grids. One of the hybridoma supernatants (2F11) produced a specific labelling of caveolae which was much more intense than that obtained with caveolin-1 antibodies. In Western blots of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) separated proteins in crude membrane fractions from different rat tissues, 2F11 labelled a band corresponding to 60 kDa. The intensity of 2F11 labelling was high in adipose tissue and in other tissues varied in parallel to caveolin- labelling. In blots of plasma membrane (PM) and light-microsomal (LM) fractions from a homogenate of adipocytes, prior insulin stimulation of the adipocytes translocated GLUT-4 from the LM to the PM fraction, but was without effect on the distribution of the 60-kDa protein labelled by 2F11. Digestion with endoproteinase lys-C produced the same pattern of immunoreactive fragments of the protein in the vesicular PM and LM fractions, indicating similar membrane topology of the 2F11-reactive, 60-kDa protein in vesicles of PM and LM fractions.


Subject(s)
Adipocytes/chemistry , Caveolins/analysis , Muscle Proteins , Adipocytes/drug effects , Adipocytes/ultrastructure , Animals , Antibodies, Monoclonal , Blotting, Western , Caveolin 1 , Caveolins/immunology , Cell Fractionation , Cell Membrane/chemistry , Glucose Transporter Type 4 , Hybridomas , Hypoglycemic Agents/pharmacology , Insulin/pharmacology , Mice , Microscopy, Electron , Microscopy, Immunoelectron , Microsomes/chemistry , Monosaccharide Transport Proteins/analysis , Monosaccharide Transport Proteins/immunology , Rats , Rats, Wistar
15.
Am J Pathol ; 158(3): 833-9, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11238032

ABSTRACT

Caveolae are plasma membrane microdomains that have been implicated in the regulation of several intracellular signaling pathways. Previous studies suggest that caveolin-1, the main structural protein of caveolae, could function as a tumor suppressor. Caveolin-1 is highly expressed in terminally differentiated mesenchymal cells including adipocytes, endothelial cells, and smooth muscle cells. To study whether caveolin-1 is a possible tumor suppressor in human mesenchymal tumors, we have analyzed the expression using immunohistochemistry in normal mesenchymal tissues, 22 benign and 79 malignant mesenchymal tumors. Caveolin-1 was found to be expressed in fibromatoses, leiomyomas, hemangiomas, and lipomas at high levels comparable to normal mesenchymal tissues. The expression of caveolin-1 was slightly reduced in four of six well-differentiated liposarcomas and strongly reduced or lost in three of three fibrosarcomas, 17 of 20 leiomyosarcomas, 16 of 16 myxoid/round cell/pleomorphic liposarcomas, five of eight angiosarcomas, 15 of 18 malignant fibrous histiocytomas, and eight of eight synovial sarcomas. The immunohistochemical findings were confirmed by Western blot analysis in a number of tumors. High levels of both the 24-kd [alpha]- and the 21-kd [beta]-isoform of caveolin-1 were detected in the nontumorigenic human fibroblast cell line IMR-90. In contrast, in HT-1080 human fibrosarcoma cells, caveolin-1 is strongly down-regulated. We show that the [alpha]-isoform of caveolin-1 is potently up-regulated in HT-1080 cells by inhibition of the mitogen-activated protein kinase-signaling pathway with the specific inhibitor PD 98059, whereas the specific inhibitor of DNA methylation 5-aza-2'-deoxycytidine only marginally up-regulates caveolin-1. In addition, re-expression of caveolin-1 in HT-1080 fibrosarcoma cells potently inhibited colony formation. From these we conclude that caveolin-1 is likely to act as a tumor suppressor gene in human sarcomas.


Subject(s)
Caveolins/genetics , Sarcoma/genetics , Blotting, Western , Caveolin 1 , Caveolins/immunology , Caveolins/metabolism , Cell Division , Cell Line , Down-Regulation/drug effects , Enzyme Inhibitors/pharmacology , Flavonoids/pharmacology , Genes, Tumor Suppressor , Humans , Immunohistochemistry , Neoplasms, Adipose Tissue/metabolism , Neoplasms, Fibrous Tissue/metabolism , Neoplasms, Muscle Tissue/metabolism , Neoplasms, Vascular Tissue/metabolism , Sarcoma/metabolism , Sarcoma/pathology , Transfection , Tumor Cells, Cultured
16.
Mol Endocrinol ; 14(11): 1750-75, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11075810

ABSTRACT

Caveolin-1 was first identified as a phosphoprotein in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. Tyrosine 14 is now thought to be the principal site for recognition by c-Src kinase; however, little is known about this phosphorylation event. Here, we generated a monoclonal antibody (mAb) probe that recognizes only tyrosine 14-phosphorylated caveolin-1. Using this approach, we show that caveolin-1 (Y14) is a specific tyrosine kinase substrate that is constitutively phosphorylated in Src- and Abl-transformed cells and transiently phosphorylated in a regulated fashion during growth factor signaling. We also provide evidence that tyrosine-phosphorylated caveolin-1 is localized at the major sites of tyrosine-kinase signaling, i.e. focal adhesions. By analogy with other signaling events, we hypothesized that caveolin-1 could serve as a docking site for pTyr-binding molecules. In support of this hypothesis, we show that phosphorylation of caveolin-1 on tyrosine 14 confers binding to Grb7 (an SH2-domain containing protein) both in vitro and in vivo. Furthermore, we demonstrate that binding of Grb7 to tyrosine 14-phosphorylated caveolin-1 functionally augments anchorage-independent growth and epidermal growth factor (EGF)-stimulated cell migration. We discuss the possible implications of our findings in the context of signal transduction.


Subject(s)
Caveolins/metabolism , Growth Substances/metabolism , Proteins/metabolism , Tyrosine/metabolism , src-Family Kinases/metabolism , 3T3 Cells , Adipocytes/drug effects , Amino Acid Sequence , Animals , Antibodies, Monoclonal/pharmacology , Caveolae/metabolism , Caveolin 1 , Caveolins/genetics , Caveolins/immunology , Cell Adhesion/physiology , Cell Division/physiology , Cell Movement/physiology , Epidermal Growth Factor/pharmacology , ErbB Receptors/metabolism , Female , GRB7 Adaptor Protein , Humans , Insulin/metabolism , Insulin/pharmacology , Lipid Metabolism , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Phosphorylation/drug effects , Signal Transduction , Vanadates/pharmacology
17.
J Cell Sci ; 113 Pt 19: 3509-17, 2000 Oct.
Article in English | MEDLINE | ID: mdl-10984441

ABSTRACT

The relationship between caveolin-1 isoforms alpha and beta and caveolar ultrastructure was studied. By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled by an antibody recognizing both isoforms (anti-alphabeta) were not labeled by another antibody specific for the alpha isoform (anti-alpha). Immunogold electron microscopy of freeze-fracture replicas revealed caveolae of different depths, and indicated that anti-alpha labeled deep caveolae preferentially over shallow ones, whereas anti-alphabeta labeled both forms with an equivalent frequency and intensity. The presence of the beta isoform in deep caveolae was confirmed by labeling epitope-tagged beta-caveolin. When made to be expressed in HepG2 cells lacking endogenous caveolins, the alpha isoform formed caveolar depressions efficiently, but the beta isoform hardly did so. Caveolae were also formed in cells expressing the two isoforms, but their frequency was variable among cells of the same clone. Coexpression of caveolin-1 and caveolin-2 caused more efficient formation of deep caveolae than caveolin-1 alone. The result indicates that the two isoforms of caveolin-1 have a different potential for forming caveolae structure, and more importantly, that deep and shallow caveolae may be diversified in their molecular composition.


Subject(s)
Caveolae/metabolism , Caveolae/ultrastructure , Caveolins/metabolism , Adult , Animals , Blotting, Western , CHO Cells , Caveolae/chemistry , Caveolin 1 , Caveolins/chemistry , Caveolins/genetics , Caveolins/immunology , Cell Extracts , Cells, Cultured , Cricetinae , DNA, Complementary/metabolism , Fibroblasts , Freeze Fracturing , Humans , Immunohistochemistry , Mice , Microscopy, Fluorescence , Microscopy, Immunoelectron , Protein Isoforms , RNA/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Transfection , Tumor Cells, Cultured
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