ABSTRACT
Three types of human odontogenic tumors histologically classified as compound composite odontoma, ossifying fibroma, and Pindborg tumor were characterized using mid-infrared spectroscopy (mid-IR) and solid-state nuclear magnetic resonance (ssNMR). For comparison, human jawbone and dental mineralized tissues such as dentin, enamel, and dental cement were also characterized. The studies focused on the structural properties and chemical composition of pathological tissues versus histochemically related tissues. All analyzed tumors were composed of organic and mineral parts and water. Apatite was found to be the main constituent of the mineral part. Various components (water, structural hydroxyl groups, carbonate ions (CO(3)(2-)), and hydrogen phosphate ions (HPO(4)(2-))) and physicochemical parameters (index of apatite maturity and crystallinity) were examined. The highest organic/mineral ratio was observed in fibrocementoma, a finding that can be explained by the fibrous character of the tumor. The lowest relative HPO(4)(2-) content was found in odontoma. This tumor is characterized by the highest mineral crystallinity index and content of structural hydroxyl groups. The Pindborg tumor mineral portion was found to be poorly crystalline and rich in HPO(4)(2-). The relative CO(3)(2-) content was similar in all samples studied. The results of spectroscopic studies of odontogenic tumors were consistent with the standard histochemical analysis. It was shown that the various techniques of ssNMR and elaborate analysis of the mid-IR spectra, applied together, provide valuable information about calcified benign odontogenic tumors.
Subject(s)
Apatites/analysis , Bone Neoplasms/chemistry , Fibroma, Ossifying/chemistry , Nuclear Magnetic Resonance, Biomolecular/methods , Odontogenic Tumors/chemistry , Spectroscopy, Fourier Transform Infrared/methods , Adolescent , Adult , Bone Neoplasms/classification , Bone Neoplasms/pathology , Carbonates/analysis , Cementoma/chemistry , Cementoma/pathology , Child , Dental Cementum/chemistry , Dental Enamel/chemistry , Dentin/chemistry , Fibroma/chemistry , Fibroma/pathology , Fibroma, Ossifying/classification , Fibroma, Ossifying/pathology , Humans , Jaw/chemistry , Middle Aged , Molar/chemistry , Odontogenic Cyst, Calcifying/chemistry , Odontogenic Cyst, Calcifying/pathology , Odontogenic Tumors/classification , Odontogenic Tumors/pathology , Odontoma/chemistry , Odontoma/pathology , Phosphates/analysis , Water/analysisABSTRACT
OBJECTIVE: To clarify the role of immunohistochemistry in the diagnosis of psammomatoid ossifying fibroma (PSOF), conventional cemento-ossifying fibroma (COF), and psammomatous meningioma (PM) of the craniofacial skeleton. STUDY DESIGN: The histology and immunohistochemistry of 4 PSOFs, 6 COFs, and 7 PMs was studied. Antibodies included EMA, cytokeratins, smooth muscle actin (SMA), desmin, vimentin, CD34, CD10, S-100 protein, and glial fibrillary acidic protein (GFAP). RESULTS: All PSOFs showed multiple round ossicles homogeneously distributed within a fibroblastic stroma. Psammomatous meningiomas had meningothelial features. All tumors, except 1 COF, were positive for EMA. All of them expressed vimentin, and none showed cytokeratins. Staining for SMA and S-100 protein was variable. CD10 was positive in all cases except 2 meningiomas. CD34 and GFAP stained only 1 case of meningioma each. CONCLUSIONS: The diagnosis of PSOF should rest on histologic features. An incorrect diagnosis of meningioma based on the expression of EMA should be avoided.
Subject(s)
Bone Neoplasms/pathology , Ethmoid Sinus/pathology , Fibroma, Ossifying/pathology , Paranasal Sinus Neoplasms/pathology , Sphenoid Sinus/pathology , Actins/analysis , Adolescent , Adult , Antigens, CD34/analysis , Bone Neoplasms/chemistry , Cementoma/chemistry , Cementoma/diagnosis , Child , Diagnosis, Differential , Female , Fibroma, Ossifying/chemistry , Humans , Immunoenzyme Techniques , Jaw Neoplasms/chemistry , Jaw Neoplasms/diagnosis , Male , Meningeal Neoplasms/chemistry , Meningeal Neoplasms/diagnosis , Meningioma/chemistry , Meningioma/diagnosis , Middle Aged , Mucin-1/analysis , Paranasal Sinus Neoplasms/chemistry , Vimentin/analysisABSTRACT
OBJECTIVE: Differential diagnosis of cementifying fibroma, ossifying fibroma and fibrous dysplasia by histological evaluation is often difficult. The aim of this study was to examine the immunoreactivities for keratan sulfate (KS) and chondroitin-4-sulfate (C4S) glycosaminoglycans of the histological samples obtained from mandibles of patients with these diseases. MATERIALS AND METHODS: The samples were collected from three patients with cementifying fibroma, two with ossifying fibroma and three with fibrous dysplasia and were subjected to immunohistochemical analyses. RESULTS: The results demonstrated that a significant immunoreactivity for KS was found in lacunae housing cells in the cementum-particles of cementifying fibromas, while both ossifying fibromas and fibrous dysplasias showed no significant immunoreactivity for KS. For C4S, while the former showed little immunoreactivity, the latter two cases exhibited intensive immunostaining in the pre- and poorly mineralized matrices. CONCLUSIONS: These results suggest that cementifying fibromas could be distinguished from these fibro-osseous tumors by using immunohistochemical analysis for KS and C4S.
