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1.
Life Sci Alliance ; 2(5)2019 10.
Article in English | MEDLINE | ID: mdl-31649152

ABSTRACT

Differences in immune responses across species can contribute to the varying permissivity of species to the same viral pathogen. Understanding how our closest evolutionary relatives, nonhuman primates (NHPs), confront pathogens and how these responses have evolved over time could shed light on host range barriers, especially for zoonotic infections. Here, we analyzed cell-intrinsic immunity of primary cells from the broadest panel of NHP species interrogated to date, including humans, great apes, and Old and New World monkeys. Our analysis of their transcriptomes after poly(I:C) transfection revealed conservation in the functional consequences of their response. In mapping reads to either the human or the species-specific genomes, we observed that with the current state of NHP annotations, the percent of reads assigned to a genetic feature was largely similar regardless of the method. Together, these data provide a baseline for the cell-intrinsic responses elicited by a potent immune stimulus across multiple NHP donors, including endangered species, and serve as a resource for refining and furthering the existing annotations of NHP genomes.


Subject(s)
Cercopithecidae/genetics , Gene Expression Profiling/methods , Hominidae/genetics , Immunity, Cellular/drug effects , Platyrrhini/genetics , Poly I-C/administration & dosage , Sequence Analysis, RNA/methods , Animals , Cells, Cultured , Cercopithecidae/immunology , Conserved Sequence , Evolution, Molecular , Female , Gene Expression Regulation/drug effects , Gene Regulatory Networks/drug effects , Hominidae/immunology , Humans , Male , Mice , Molecular Sequence Annotation , Platyrrhini/immunology , Poly I-C/pharmacology
2.
Front Immunol ; 9: 2862, 2018.
Article in English | MEDLINE | ID: mdl-30568659

ABSTRACT

Viral infections trigger robust secretion of interferons and other antiviral cytokines by infected and bystander cells, which in turn can tune the immune response and may lead to viral clearance or immune suppression. However, aberrant or unrestricted cytokine responses can damage host tissues, leading to organ dysfunction, and even death. To understand the cytokine milieu and immune responses in infected host tissues, non-human primate (NHP) models have emerged as important tools. NHP have been used for decades to study human infections and have played significant roles in the development of vaccines, drug therapies and other immune treatment modalities, aided by an ability to control disease parameters, and unrestricted tissue access. In addition to the genetic and physiological similarities with humans, NHP have conserved immunologic properties with over 90% amino acid similarity for most cytokines. For example, human-like symptomology and acute respiratory syndrome is found in cynomolgus macaques infected with highly pathogenic avian influenza virus, antibody enhanced dengue disease is common in neotropical primates, and in NHP models of viral hepatitis cytokine-induced inflammation induces severe liver damage, fibrosis, and hepatocellular carcinoma recapitulates human disease. To regulate inflammation, anti-cytokine therapy studies in NHP are underway and will provide important insights for future human interventions. This review will provide a comprehensive outline of the cytokine-mediated exacerbation of disease and tissue damage in NHP models of viral infections and therapeutic strategies that can aid in prevention/treatment of the disease syndromes.


Subject(s)
Cercopithecidae/immunology , Cytokines/metabolism , Hominidae/immunology , Platyrrhini/immunology , Virus Diseases/immunology , Animals , Cytokines/immunology , Disease Models, Animal , Disease Progression , Drug Evaluation, Preclinical/methods , Humans , Immunotherapy/methods , Virus Diseases/pathology , Virus Diseases/therapy , Virus Diseases/virology
3.
Nat Rev Immunol ; 18(6): 390-404, 2018 06.
Article in English | MEDLINE | ID: mdl-29556017

ABSTRACT

Humans have a close phylogenetic relationship with nonhuman primates (NHPs) and share many physiological parallels, such as highly similar immune systems, with them. Importantly, NHPs can be infected with many human or related simian viruses. In many cases, viruses replicate in the same cell types as in humans, and infections are often associated with the same pathologies. In addition, many reagents that are used to study the human immune response cross-react with NHP molecules. As such, NHPs are often used as models to study viral vaccine efficacy and antiviral therapeutic safety and efficacy and to understand aspects of viral pathogenesis. With several emerging viral infections becoming epidemic, NHPs are proving to be a very beneficial benchmark for investigating human viral infections.


Subject(s)
Primates/immunology , Virus Diseases/immunology , Animal Experimentation/ethics , Animals , Cercopithecidae/genetics , Cercopithecidae/immunology , Cercopithecidae/virology , DNA Virus Infections/genetics , DNA Virus Infections/immunology , DNA Virus Infections/virology , Disease Models, Animal , Hominidae/genetics , Hominidae/immunology , Hominidae/virology , Humans , Models, Immunological , Platyrrhini/genetics , Platyrrhini/immunology , Platyrrhini/virology , Primates/genetics , Primates/virology , RNA Virus Infections/genetics , RNA Virus Infections/immunology , RNA Virus Infections/virology , Species Specificity , Virus Diseases/genetics , Virus Diseases/virology
4.
J Virol ; 89(17): 9133-6, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26085168

ABSTRACT

Epstein-Barr-related herpesviruses, or lymphocryptoviruses (LCV), naturally infect humans and nonhuman primates (NHP), but their host range is not well characterized. Using LCV and B cells from multiple species of Hominidae and Cercopithecidae, we show that LCV can immortalize B cells from some nonnative species but that growth transformation is restricted to B cells from their own family of hominoids or Old World NHP, suggesting a high degree of LCV adaptation to their natural primate host.


Subject(s)
Cercopithecidae/virology , Herpesvirus 4, Human/pathogenicity , Host Specificity , Lymphocryptovirus/pathogenicity , Monkey Diseases/virology , Amino Acid Sequence , Animals , B-Lymphocytes/immunology , B-Lymphocytes/virology , Base Sequence , Cercopithecidae/immunology , Epstein-Barr Virus Nuclear Antigens/genetics , Herpesviridae Infections/pathology , Herpesviridae Infections/virology , Humans , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, DNA , Viral Proteins/genetics
5.
ILAR J ; 54(2): 181-95, 2013.
Article in English | MEDLINE | ID: mdl-24174441

ABSTRACT

Next-generation sequencing technologies have led to rapid progress in the fields of human and nonhuman primate (NHP) genomics. The less expensive and more efficient technologies have enabled the sequencing of human genomes from multiple populations and the sequencing of many NHP species. NHP genomes have been sequenced for two main reasons: (1) their importance as animal models in biomedical research and (2) their phylogenetic relationship to humans and use in derivative evolutionary studies. NHPs are valuable animal models for a variety of diseases, most notably for human immunodeficiency virus/acquired immunodeficiency syndrome research, and for vaccine development. Knowledge about the variation in primate immune response loci can provide essential insights into relevant immune function. However, perhaps ironically considering their central role in infectious disease, the accumulation of sequence detail from genomic regions harboring immune response loci, such as the major histocompatibility complex and killer immunoglobulin-like receptors, has been slow. This deficiency is, at least in part, due to the highly repetitive and polymorphic nature of these regions and is being addressed by the application of special approaches to targeted sequencing of the immune response genomic regions. We discuss one such targeting approach that has successfully yielded complete phased genomic sequences from complex genomic regions and is now being used to resequence macaque and other primate major histocompatibility complex regions. The essential detail contained within the genomics of the NHP immune response is now being assembled, and the realization of precise comparisons between NHP and human immune genomics is close at hand, further enhancing the NHP animal model in the search for effective treatments for human disease.


Subject(s)
Cercopithecidae/genetics , Genetic Variation/genetics , Genomics/methods , HIV Infections/genetics , HIV/genetics , Polymorphism, Genetic/genetics , Animals , Cercopithecidae/immunology , Disease Models, Animal , Genetic Variation/immunology , HIV/immunology , HIV Infections/immunology , Humans , Major Histocompatibility Complex/genetics , Major Histocompatibility Complex/immunology , Polymorphism, Genetic/immunology
6.
PLoS One ; 8(5): e64936, 2013.
Article in English | MEDLINE | ID: mdl-23717676

ABSTRACT

The expression of killer cell immunoglobulin-like receptors (KIR) on lymphocytes of rhesus macaques and other Old World monkeys was unknown so far. We used our recently established monoclonal anti-rhesus macaque KIR antibodies in multicolour flow cytometry for phenotypic characterization of KIR protein expression on natural killer (NK) cells and T cell subsets of rhesus macaques, cynomolgus macaques, hamadryas baboons, and African green monkeys. Similar to human KIR, we found clonal expression patterns of KIR on NK and T cell subsets in rhesus macaques and differences between individuals using pan-KIR3D antibody 1C7 and antibodies specific for single KIR. Similar results were obtained with lymphocytes from the other studied species. Notably, African green monkeys show only a low frequency of KIR3D expressed on CD8+ αßT cells. Contrasting human NK cells are KIR-positive CD56bright NK cells and frequencies of KIR-expressing NK cells that are independent of the presence of their cognate MHC class I ligands in rhesus macaques. Interestingly, the frequency of KIR-expressing cells and the expression strength of KIR3D are correlated in γδ T cells of rhesus macaques and CD8+ αßT cells of baboons.


Subject(s)
Cercopithecidae/immunology , Killer Cells, Natural/immunology , Receptors, KIR/metabolism , T-Lymphocyte Subsets , T-Lymphocytes/immunology , Animals , Flow Cytometry , Histocompatibility Antigens Class I/immunology , Ligands , Receptors, KIR/immunology
7.
Lab Anim ; 47(2): 100-5, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23563364

ABSTRACT

Some biomedical research procedures, such as organ xenotransplantation, usually require intensive hemotherapy. Knowledge of the whole phenotype of blood donor and graft could be useful in the field of xenotransplantation. Human and simian-type categories of blood groups have been established and they can be tested by standard methods used for human blood grouping. The aim of this work was to study the incidence of non-ABO blood group systems in different species of non-human primates, which are employed in biomedical research. The phenotype of Rh, Lewis, Kidd, Kell, MNSs, Lutheran, P and Duffy antigens was investigated in olive baboon (n = 48), chacma baboon (n = 9), Guinea baboon (n = 14), Rhesus macaque (n = 38) and squirrel monkey (n = 30) by using commercial microtyping cards. Kell, Lutheran, Kidd and Duffy antigens have been detected in all species, Rh in squirrel monkey, MNSs in rhesus macaque and squirrel monkey, and Lewis in baboon and rhesus macaque. There were differences in frequency and haemagglutination scores between species regardless of their gender and age. The main differences were found in squirrel monkey when compared with baboons and macaques. This typing system provides a tool to assess the presence of antigens in animals used for experimental procedures, such as xenotransplantation and xenotransfusion.


Subject(s)
Blood Group Antigens/genetics , Blood Grouping and Crossmatching , Cercopithecidae/immunology , Erythrocytes/immunology , Saimiri/immunology , Transplantation, Heterologous , Aging , Animals , Blood Banks , Cercopithecidae/blood , Female , Hemagglutination Tests , Immunophenotyping , Male , Phenotype , Saimiri/blood , Sex Characteristics , Species Specificity
8.
Gene ; 474(1-2): 39-51, 2011 Mar 15.
Article in English | MEDLINE | ID: mdl-21185924

ABSTRACT

Killer cell Ig-like receptors (KIRs) modulate the cytotoxic effects of Natural Killer cells. KIR genes are encoded in the Leucocyte Receptor Complex and are characterized by their high haplotypic diversity and polymorphism. The KIR system has been studied in only three species of Old World monkeys, the rhesus macaque, the cynomolgus macaque, and the sabaeus monkey, displaying a complexity rivaling that of hominids (human and apes). Here we analyzed bacterial artificial chromosome draft sequences spanning the KIR haplotype of three other Old World monkeys, the vervet monkey (Chlorocebus aethiops), the olive baboon (Papio anubis) and the colobus monkey (Colobus guereza). A total of 25 KIR gene models were identified in these species, predicted to encode receptors with 1, 2, and 3 extracellular Ig domains, all of them with long cytoplasmic domains having two putative ITIMs, although three had a positively charged residue in the transmembrane domain. Sequence and phylogenetic analyses showed that most Old World monkeys shared five classes of KIR loci: i) KIR2DL5/3DL20 in the most centromeric region, followed by ii) the single Ig domain-encoding locus KIR1D, iii) the pseudogene KIR2DP, iv) the conserved KIR2DL4, and v) the highly diversified KIR3DL/H loci in the telomeric half of the cluster. An exception to this pattern was the KIR haplotype of the colobus monkey that lacked the KIR1D, KIR2DP, and KIR2DL4 loci of the central region of the cluster. Thus, Old World monkeys display a broad spectrum of KIR haplotype variation that has been generated upon an ancestral haplotype architecture by gene duplication, gene deletion, and non-homologous recombination.


Subject(s)
Cercopithecidae/genetics , Evolution, Molecular , Killer Cells, Natural/immunology , Receptors, KIR/genetics , Amino Acid Sequence , Animals , Base Sequence , Cercopithecidae/immunology , Colobus/genetics , Colobus/immunology , Conserved Sequence , Gene Deletion , Gene Duplication , Genetic Loci , Haplotypes , Molecular Sequence Data , Papio anubis/genetics , Papio anubis/immunology , Phylogeny , Pseudogenes , Receptors, KIR/classification , Receptors, KIR/immunology , Recombination, Genetic
9.
Exp Gerontol ; 45(9): 655-61, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20558288

ABSTRACT

Aging is accompanied by a general dysregulation in immune system function, commonly referred to as 'immune senescence'. This progressive deterioration affects both innate and adaptive immunity, although accumulating evidence indicates that the adaptive arm of the immune system may exhibit more profound changes. Most of our current understanding of immune senescence stems from clinical and rodent studies. More recently, the use of nonhuman primates (NHPs) to investigate immune senescence and test interventions aimed at delaying/reversing age-related changes in immune function has dramatically increased. These studies have been greatly facilitated by several key advances in our understanding of the immune system of old world monkeys, specifically the rhesus macaques. In this review we describe the hallmarks of immune senescence in this species and compare them to those described in humans. We also discuss the impact of immune senescence on the response to vaccination and the efficacy of immuno-restorative interventions investigated in this model system.


Subject(s)
Aging/immunology , Primates/growth & development , Primates/immunology , Animals , Cercopithecidae/growth & development , Cercopithecidae/immunology , Energy Intake , Fibroblast Growth Factor 7/physiology , Humans , Immune System/growth & development , Immune System/physiology , Inflammation/immunology , Inflammation/veterinary , Interleukin-7/therapeutic use , Macaca mulatta/growth & development , Macaca mulatta/immunology , Rejuvenation/physiology , Thymosin/physiology
10.
J Med Primatol ; 33(2): 65-9, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15061718

ABSTRACT

The Indian langur monkey (Presbytis entellus) is an experimental host for a range of human diseases and for the assessment of vaccine candidate antigens to some common parasitic infections. This experimental host is particularly suitable for the follow-up of immunological responses. To understand some of the mechanism that underlies the defense against experimental pathogens there is a need of the basic knowledge on antibody and cell mediated immune responses. In the present study 25 naïve monkeys were subjected to for assessment of their antibody responses to various human parasitic antigens as well as mitogen induced cellular responses. Only few monkeys were found to have low titer of antiparasitic antibodies. There was compressive dose dependent proliferative response of peripheral blood mononuclear cells. Unlike humans, the blastogenic as well as cytokine responses (IFN-gamma, IL-2 and IL-4) to Con A was considerably higher as compared to PHA. These findings are similar to what have been reported in other non-human primates, confirming the appropriateness of Indian langurs for pre-clinical trials.


Subject(s)
Antibodies, Helminth/immunology , Antibodies, Protozoan/immunology , Cercopithecidae/immunology , Disease Models, Animal , Leishmaniasis, Visceral/immunology , Animals , Antibodies, Helminth/blood , Antibodies, Monoclonal/immunology , Antibodies, Protozoan/blood , Cercopithecidae/blood , Cercopithecidae/parasitology , Cytokines/immunology , Enzyme-Linked Immunosorbent Assay , Immunity, Cellular/immunology , India , Leukocytes, Mononuclear/immunology , Male , Mitogens/immunology
11.
Arch Virol ; 147(5): 929-41, 2002 May.
Article in English | MEDLINE | ID: mdl-12021865

ABSTRACT

The extent of antibody cross-reactivity of pooled antisera from rhesus monkeys, baboons, African green monkeys, langurs, sooty mangabeys and humans to 6 alphaherpesviruses (herpes B virus, herpes papio 2, simian agent 8, langur herpes virus and herpes simplex 1 & 2) was examined by two types of enzyme linked immunosorbent assays, an antibody capture assay (tELISA) and an antigen capture assay (dELISA). Percent cross-reactivity was calculated for each serum by comparison of the homologous reaction (100%) to the reaction with heterologous viruses. Comparison of the immunological reactivity of the mangabey antiserum pool to the panel of alphaherpesviruses indicated that these antibodies were induced by a yet unidentified alphaherpesvirus. In general, monkey sera were more cross-reactive to monkey herpesviruses than to human herpesviruses.However, the extent of cross-reactivity of monkey sera to human herpesviruses was relatively lower than the cross-reactivity of human sera to monkey herpes-viruses. Because of this phenomenon of "one-way" cross-reactivity that was also observed within the group of simian herpesviruses, it was difficult to rank the immunologic distances between the viruses in absolute terms.


Subject(s)
Alphaherpesvirinae/immunology , Antibodies, Viral/analysis , Immune Sera/immunology , Primates/immunology , Animals , Cercocebus atys/immunology , Cercopithecidae/immunology , Chlorocebus aethiops/immunology , Cross Reactions , Enzyme-Linked Immunosorbent Assay , Humans , Macaca mulatta/immunology , Papio/immunology , Species Specificity
12.
Virology ; 260(1): 116-24, 1999 Jul 20.
Article in English | MEDLINE | ID: mdl-10405363

ABSTRACT

We have identified a novel lentivirus prevalent in talapoin monkeys (Myopithecus talapoin), extending previous observations of human immunodeficiency virus-1 cross-reactive antibodies in the serum of these monkeys. We obtained a virus isolate from one of three seropositive monkeys initially available to us. The virus was tentatively named simian immunodeficiency virus from talapoin monkeys (SIVtal). Despite the difficulty of isolating this virus, it was readily passed between monkeys in captivity through unknown routes of transmission. The virus could be propagated for short terms in peripheral blood mononuclear cells of talapoin monkeys but not in human peripheral blood mononuclear cells or human T cell lines. The propagated virus was used to infect a naive talapoin monkey, four rhesus macaques (M. mulatta), and two cynomolgus macaques (M. fascicularis). All animals seroconverted and virus could be reisolated during a short period after experimental infection. A survey of SIVtal-infected captive talapoin monkeys revealed a relative decrease in CD4(+) cell numbers in chronically (>2 years) infected animals. No other signs of immunodeficiency were observed in any of the infected animals. PCR amplification followed by DNA sequencing of two fragments of the polymerase gene revealed that SIVtal is different from the presently known lentiviruses and perhaps most related to the SIV from Sykes monkeys.


Subject(s)
Cercopithecidae/virology , HIV-1/immunology , Simian Immunodeficiency Virus/isolation & purification , Animals , CD4 Antigens/analysis , CD8 Antigens/analysis , Cells, Cultured , Cercopithecidae/immunology , Clone Cells , Cross Reactions , Disease Transmission, Infectious , HIV Antibodies/immunology , HIV Seropositivity/immunology , Housing, Animal , Humans , Leukocytes, Mononuclear/virology , Macaca mulatta , Microscopy, Electron , Molecular Sequence Data , Simian Acquired Immunodeficiency Syndrome/immunology , Simian Acquired Immunodeficiency Syndrome/transmission , Simian Immunodeficiency Virus/classification , Simian Immunodeficiency Virus/genetics , Simian Immunodeficiency Virus/immunology , Virus Cultivation/methods
13.
Parasitology ; 116 ( Pt 3): 219-21, 1998 Mar.
Article in English | MEDLINE | ID: mdl-9550214

ABSTRACT

The protective potential of killed Leishmania major (ALM) along with BCG was evaluated against L. donovani in Indian langur monkeys in single and triple dose schedules. A delayed protection was observed in monkeys after a single dose schedule of ALM (3 mg)+BCG (3 mg) given intradermally 2 months before intravenous challenge with L. donovani. Triple dose schedule each of 1 mg ALM + 1 mg BCG was more effective. The status remained unchanged until the end of the experiment (approximately 8 months). The study indicates that a combination of ALM + BCG may be a good candidate vaccine for exploiting against human Kala-azar.


Subject(s)
Adjuvants, Immunologic , BCG Vaccine , Cercopithecidae/parasitology , Leishmania donovani/immunology , Leishmaniasis, Visceral/prevention & control , Protozoan Vaccines , Vaccination/methods , Adjuvants, Immunologic/administration & dosage , Animals , BCG Vaccine/administration & dosage , Cercopithecidae/immunology , Cricetinae , Disease Models, Animal , Dose-Response Relationship, Immunologic , India , Leishmania major/immunology , Male , Protozoan Vaccines/administration & dosage , Spleen/parasitology , Vaccines, Inactivated/administration & dosage
15.
J Exp Med ; 182(5): 1345-55, 1995 Nov 01.
Article in English | MEDLINE | ID: mdl-7595205

ABSTRACT

Type C retroviruses endogenous to various nonprimate species can infect human cells in vitro, yet the transmission of these viruses to humans is restricted. This has been attributed to direct binding of the complement component C1q to the viral envelope protein p15E, which leads to classical pathway-mediated virolysis in human serum. Here we report a novel mechanism of complement-mediated type C retrovirus inactivation that is initiated by the binding of "natural antibody" [Ab] (anti-alpha-galactosyl Ab) to the carbohydrate epitope Gal alpha 1-3Gal beta 1-4GlcNAc-R expressed on the retroviral envelope. Complement-mediated inactivation of amphotropic retroviral particles was found to be restricted to human and other Old World primate sera, which parallels the presence of anti-alpha-galactosyl natural Ab. Blockade or depletion of anti-alpha-galactosyl Ab in human serum prevented inactivation of both amphotropic and ecotropic murine retroviruses. Similarly, retrovirus was not killed by New World primate serum except in the presence of exogenous anti-alpha-galactosyl Ab. Enzyme-linked immunosorbent assays revealed that the alpha-galactosyl epitope was expressed on the surface of amphotropic and ecotropic retroviruses, and Western blot analysis further localized this epitope to the retroviral envelope glycoprotein gp70. Finally, down-regulation of this epitope on the surface of murine retroviral particle producer cells rendered them, as well as the particles liberated from these cells, resistant to inactivation by human serum complement. Our data suggest that anti-alpha-galactosyl Ab may provide a barrier for the horizontal transmission of retrovirus from species that express the alpha-galactosyl epitope to humans and to other Old World primates. Further, these data provide a mechanism for the generation of complement-resistant retroviral vectors for in vivo gene therapy applications where exposure to human complement is unavoidable.


Subject(s)
Antibodies, Viral/blood , Antigens, Viral/immunology , Blood/virology , Cebidae/immunology , Cercopithecidae/immunology , Epitopes/immunology , Galactose/immunology , Leukemia Virus, Murine/physiology , Retroviridae Proteins, Oncogenic/immunology , Viral Envelope Proteins/immunology , 3T3 Cells , Animals , Antibodies, Viral/immunology , Antigens, Viral/biosynthesis , Blood/immunology , Carbohydrate Sequence , Cebidae/blood , Cercopithecidae/blood , Complement System Proteins/immunology , Humans , Immunity, Innate , Mammals/blood , Mammals/immunology , Mice , Molecular Sequence Data , Moloney murine leukemia virus/immunology , Retroviridae Proteins, Oncogenic/biosynthesis , Species Specificity , Viral Envelope Proteins/biosynthesis
17.
Springer Semin Immunopathol ; 15(2-3): 155-71, 1993.
Article in English | MEDLINE | ID: mdl-7504839

ABSTRACT

Anti-Gal is a human natural antibody which interacts specifically with the mammalian carbohydrate structure Gal alpha 1-3Gal beta 1-4GlcNAc-R, termed, the alpha-galactosyl epitope. This antibody constitutes approximately 1% of circulating IgG in human serum and is produced, upon stimulation, by 1% of circulating B lymphocytes. Anti-Gal is also present as IgA antibodies in body secretions such as saliva, milk and colostrum. The antigenic source for the constant production of anti-Gal seems to be the alpha-galactosyl-like epitopes found on many bacteria of the gastrointestinal flora. Whereas anti-Gal is abundant in humans, apes and Old World monkeys, it is absent from New World monkeys, prosimians and nonprimate mammals. The latter group of species produces, however, large amounts of alpha-galactosyl epitopes (> 10(6) epitopes per cell). It is estimated that anti-Gal appeared in ancestral Old World primates less than 28 million years ago, possibly as a result of an evolutionary event which exerted a selective pressure for the suppression of alpha-galactosyl epitopes expression by inactivation of the gene for the enzyme alpha 1,3 galactosyltransferase. This also resulted in the loss of immune tolerance to the alpha-galactosyl epitope and the production of anti-Gal. The physiologic role of this antibody is not clear as yet. It may participate in the protection against gastrointestinal bacteria. In addition it seems to contribute to the removal of normal and pathologically senescent red cells by interacting with the few hundred cryptic alpha-galactosyl epitopes which are exposed de novo in the course of red cell aging, thereby opsonizing these cells for phagocytosis by reticuloendothelial macrophages. The alpha-galactosyl epitope has been found to be aberrantly expressed on human cells and the interaction of anti-Gal with such epitopes may result in autoimmune disease. Preliminary data suggest such a mechanism in Graves' disease. Anti-Gal has been found to interact with therapeutic recombinant proteins expressing alpha-galactosyl epitopes, but so far there is no indication that it affects the half-life in the circulation and the biologic activity. Detection of anti-Gal in the seminal fluid and in the cerebrospinal fluid may serve as a simple means for assessment of damage to the blood-genital tract barrier or the blood-brain barrier. Studies on the interaction of anti-Gal with aberrantly expressed alpha-galactosyl epitopes on human cells may elucidate the possible role of anti-Gal in human autoimmune diseases.


Subject(s)
Antibodies, Heterophile/immunology , Epitopes/immunology , Immunoglobulin G/immunology , Primates/immunology , Animals , Antibodies, Heterophile/genetics , Autoimmune Diseases/etiology , Autoimmune Diseases/immunology , Biological Evolution , Carbohydrate Sequence , Cercopithecidae/immunology , Enzyme Induction , Galactosyltransferases/genetics , Galactosyltransferases/metabolism , Glycolipids/immunology , Glycoproteins/immunology , Hominidae/immunology , Humans , Immunoglobulin G/genetics , Molecular Sequence Data , Oligosaccharides/immunology , Species Specificity , Trisaccharides/immunology
18.
Lipids ; 26(9): 679-83, 1991 Sep.
Article in English | MEDLINE | ID: mdl-1837065

ABSTRACT

Lipoprotein(a) [Lp(a)] is a low density lipoprotein which has apo(a) disulfide-linked to apoB100. Apo(a) has recently been shown to have a striking homology with plasminogen, a knowledge that has stimulated a lot of interest in the mechanism of atherogenicity and thrombogenicity of this lipoprotein particle. Several studies have documented the presence of Lp(a) in nonhuman primates with particular reference to the rhesus monkeys and baboons. The Lp(a) of rhesus monkey is structurally very similar to that of humans, except for the absence of kringle V and the amino acid composition of the catalytic region. The Lp(a) of nonhuman primates, like their human counterparts, exhibit a wide range of interindividual plasma levels and also a wide size polymorphism of apo(a). Nonhuman primates appear to represent a good model for the study of the structure and biology of Lp(a).


Subject(s)
Cercopithecidae/genetics , Lipoproteins/genetics , Animals , Cercopithecidae/immunology , Genetic Variation , Lipoprotein(a) , Lipoproteins/immunology , Macaca mulatta/genetics , Macaca mulatta/immunology , Papio/genetics , Papio/immunology , Pedigree , Primates/genetics , Primates/immunology , Sequence Homology, Nucleic Acid
19.
J Gen Virol ; 72 ( Pt 2): 453-6, 1991 Feb.
Article in English | MEDLINE | ID: mdl-1847180

ABSTRACT

Sera from 154 African non-human primates were screened for the presence of antibodies to type D retrovirus proteins. Four of five talapoin monkeys (Miopithecus sp.) captured in western Africa were positive for antibodies to type D retrovirus by ELISA and by immunoblot reactivity. Talapoins are the only African non-human primates that have so far shown evidence for type D retrovirus infection. Thus, talapoin monkeys appear to be a reservoir of type D retrovirus infection.


Subject(s)
Antibodies, Viral/blood , Cercopithecidae/immunology , Monkey Diseases/epidemiology , Retroviruses, Simian/immunology , Tumor Virus Infections/veterinary , Africa, Western , Animals , Disease Reservoirs , Enzyme-Linked Immunosorbent Assay , Immunoblotting , Tumor Virus Infections/epidemiology
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