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1.
eNeuro ; 5(6)2018.
Article in English | MEDLINE | ID: mdl-30460331

ABSTRACT

Neural stem cells (NSCs) hold great promise for neural repair in cases of CNS injury and neurodegeneration; however, conventional cell-based transplant methods face the challenges of poor survival and inadequate neuronal differentiation. Here, we report an alternative, tissue-based transplantation strategy whereby cerebral organoids derived from human pluripotent stem cells (PSCs) were grafted into lesioned mouse cortex. Cerebral organoid transplants exhibited enhanced survival and robust vascularization from host brain as compared to transplants of dissociated neural progenitor cells (NPCs). Engrafted cerebral organoids harbored a large NSC pool and displayed multilineage neurodifferentiation at two and four weeks after grafting. Cerebral organoids therefore represent a promising alternative source to NSCs or fetal tissues for transplantation, as they contain a large set of neuroprogenitors and differentiated neurons in a structured organization. Engrafted cerebral organoids may also offer a unique experimental paradigm for modeling human neurodevelopment and CNS diseases in the context of vascularized cortical tissue.


Subject(s)
Brain Injuries/surgery , Pluripotent Stem Cells/physiology , Stem Cell Transplantation/methods , Animals , Animals, Newborn , Cell Differentiation , Cell Proliferation , Cells, Cultured , Cerebral Cortex/transplantation , Disease Models, Animal , Doublecortin Domain Proteins , Female , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Humans , Ki-67 Antigen/metabolism , Male , Mice , Microtubule-Associated Proteins/genetics , Microtubule-Associated Proteins/metabolism , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Neuropeptides/genetics , Neuropeptides/metabolism , Pluripotent Stem Cells/transplantation , Transfection
2.
Sci Rep ; 6: 33080, 2016 09 12.
Article in English | MEDLINE | ID: mdl-27615195

ABSTRACT

Transplantation of embryonic cortical tissue is considered as a promising therapy for brain injury. Grafted neurons can reestablish neuronal network and improve cortical function of the host brain. Microglia is a key player in regulating neuronal survival and plasticity, but its activation and dynamics in grafted cortical tissue remain unknown. Using two-photon intravital imaging and parabiotic model, here we investigated the proliferation and source of microglia in the donor region by transplanting embryonic cortical tissue into adult cortex. Live imaging showed that the endogenous microglia of the grafted tissue were rapidly lost after transplantation. Instead, host-derived microglia infiltrated and colonized the graft. Parabiotic model suggested that the main source of infiltrating cells is the parenchyma of the host brain. Colonized microglia proliferated and experienced an extensive morphological transition and eventually differentiated into resting ramified morphology. Collectively, these results demonstrated that donor tissue has little contribution to the activated microglia and host brain controls the microglial population in the graft.


Subject(s)
Brain Tissue Transplantation , Cerebral Cortex/physiology , Cerebral Cortex/transplantation , Fetal Tissue Transplantation , Microglia/physiology , Animals , Cell Proliferation , Cerebral Cortex/cytology , Female , Male , Mice, Inbred C57BL , Mice, Transgenic , Microglia/cytology
3.
Stem Cells Dev ; 23(18): 2129-42, 2014 Sep 15.
Article in English | MEDLINE | ID: mdl-24325299

ABSTRACT

Current stem cell technologies have enabled the induction of cortical progenitors and neurons from embryonic stem cells (ESCs) and induced pluripotent stem cells in vitro. To understand the mechanisms underlying the acquisition of apico-basal polarity and the formation of processes associated with the stemness of cortical cells generated in monolayer culture, here, we developed a novel in utero transplantation system based on the moderate dissociation of adherens junctions in neuroepithelial tissue. This method enables (1) the incorporation of remarkably higher numbers of grafted cells and (2) quantitative morphological analyses at single-cell resolution, including time-lapse recording analyses. We then grafted cortical progenitors induced from mouse ESCs into the developing brain. Importantly, we revealed that the mode of process extension depends on the extrinsic apico-basal polarity of the host epithelial tissue, as well as on the intrinsic differentiation state of the grafted cells. Further, we successfully transplanted cortical progenitors induced from human ESCs, showing that our strategy enables investigation of the neurogenesis of human neural progenitors within the developing mouse cortex. Specifically, human cortical cells exhibit multiple features of radial migration. The robust transplantation method established here could be utilized both to uncover the missing gap between neurogenesis from ESCs and the tissue environment and as an in vivo model of normal and pathological human corticogenesis.


Subject(s)
Cell Polarity , Cerebral Cortex/cytology , Pluripotent Stem Cells/cytology , Pluripotent Stem Cells/transplantation , Animals , Cell Polarity/drug effects , Cerebral Cortex/embryology , Cerebral Cortex/transplantation , Cerebral Ventricles/embryology , Egtazic Acid/administration & dosage , Egtazic Acid/pharmacology , Epithelial Cells/cytology , Epithelial Cells/drug effects , Humans , Mice, Transgenic , Neurons/cytology , Neurons/drug effects , Pluripotent Stem Cells/drug effects
4.
Brain ; 136(Pt 12): 3561-77, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24148272

ABSTRACT

Stem cell-based approaches to restore function after stroke through replacement of dead neurons require the generation of specific neuronal subtypes. Loss of neurons in the cerebral cortex is a major cause of stroke-induced neurological deficits in adult humans. Reprogramming of adult human somatic cells to induced pluripotent stem cells is a novel approach to produce patient-specific cells for autologous transplantation. Whether such cells can be converted to functional cortical neurons that survive and give rise to behavioural recovery after transplantation in the stroke-injured cerebral cortex is not known. We have generated progenitors in vitro, expressing specific cortical markers and giving rise to functional neurons, from long-term self-renewing neuroepithelial-like stem cells, produced from adult human fibroblast-derived induced pluripotent stem cells. At 2 months after transplantation into the stroke-damaged rat cortex, the cortically fated cells showed less proliferation and more efficient conversion to mature neurons with morphological and immunohistochemical characteristics of a cortical phenotype and higher axonal projection density as compared with non-fated cells. Pyramidal morphology and localization of the cells expressing the cortex-specific marker TBR1 in a certain layered pattern provided further evidence supporting the cortical phenotype of the fated, grafted cells, and electrophysiological recordings demonstrated their functionality. Both fated and non-fated cell-transplanted groups showed bilateral recovery of the impaired function in the stepping test compared with vehicle-injected animals. The behavioural improvement at this early time point was most likely not due to neuronal replacement and reconstruction of circuitry. At 5 months after stroke in immunocompromised rats, there was no tumour formation and the grafted cells exhibited electrophysiological properties of mature neurons with evidence of integration in host circuitry. Our findings show, for the first time, that human skin-derived induced pluripotent stem cells can be differentiated to cortical neuronal progenitors, which survive, differentiate to functional neurons and improve neurological outcome after intracortical implantation in a rat stroke model.


Subject(s)
Cerebral Cortex/cytology , Induced Pluripotent Stem Cells/physiology , Infarction, Middle Cerebral Artery/surgery , Neurons/physiology , Recovery of Function/physiology , Action Potentials/drug effects , Action Potentials/physiology , Animals , Cell Differentiation/drug effects , Cells, Cultured , Cerebral Cortex/transplantation , Disease Models, Animal , Electric Stimulation , Glutaminase/metabolism , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Humans , Induced Pluripotent Stem Cells/transplantation , Infarction, Middle Cerebral Artery/pathology , Neurons/classification , Neurons/drug effects , Neurotransmitter Agents/pharmacology , Patch-Clamp Techniques , Rats , Rats, Nude , Rats, Sprague-Dawley
5.
J Neurosci ; 33(10): 4216-33, 2013 Mar 06.
Article in English | MEDLINE | ID: mdl-23467340

ABSTRACT

Neurogenesis must be properly regulated to ensure that cell production does not exceed the requirements of the growing cerebral cortex, yet our understanding of mechanisms that restrain neuron production remains incomplete. We investigated the function of microglial cells in the developing cerebral cortex of prenatal and postnatal macaques and rats and show that microglia limit the production of cortical neurons by phagocytosing neural precursor cells. We show that microglia selectively colonize the cortical proliferative zones and phagocytose neural precursor cells as neurogenesis nears completion. We found that deactivating microglia in utero with tetracyclines or eliminating microglia from the fetal cerebral cortex with liposomal clodronate significantly increased the number of neural precursor cells, while activating microglia in utero through maternal immune activation significantly decreased the number of neural precursor cells. These data demonstrate that microglia play a fundamental role in regulating the size of the precursor cell pool in the developing cerebral cortex, expanding our understanding of the mechanisms that regulate cortical development. Furthermore, our data suggest that any factor that alters the number or activation state of microglia in utero can profoundly affect neural development and affect behavioral outcomes.


Subject(s)
Cerebral Cortex/cytology , Cerebral Cortex/embryology , Cerebral Cortex/growth & development , Microglia/physiology , Neural Stem Cells/physiology , Prenatal Exposure Delayed Effects/pathology , Age Factors , Analysis of Variance , Animals , Animals, Newborn , Calcium-Binding Proteins/metabolism , Cell Count , Cerebral Cortex/transplantation , Cerebral Ventricles/cytology , Cerebral Ventricles/embryology , Cerebral Ventricles/growth & development , Embryo, Mammalian , Female , HLA-DR Antigens/metabolism , Indoles/metabolism , Lipopolysaccharide Receptors/metabolism , Lipopolysaccharides/toxicity , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Macaca , Male , Microfilament Proteins/metabolism , Microscopy, Confocal , Minocycline/pharmacology , Nerve Tissue Proteins/metabolism , Neural Stem Cells/drug effects , Neurogenesis/physiology , Nitric Oxide Synthase Type II/metabolism , Phagocytosis/physiology , Pregnancy , Prenatal Exposure Delayed Effects/chemically induced , Proliferating Cell Nuclear Antigen/metabolism , Rats , T-Box Domain Proteins/metabolism
6.
Neurosci Lett ; 531(2): 155-9, 2012 Dec 07.
Article in English | MEDLINE | ID: mdl-23127849

ABSTRACT

Cell transplantation may be an effective therapeutic strategy for many neurodegenerative diseases. However, difficulty in obtaining a sufficient amount of donor cells and low graft survival are two major limiting factors. Dissociation of cells from tissues or culture is an inevitable step for cell transplantation, and cell viability in suspension may influence the outcome of the cell therapy. To this end, we asked whether the suspension time of freshly dissociated neurons in vitro affects their viability. Following 4-24h cell suspension, primary cortical neurons underwent cell death. Interestingly, the neurons exhibited only marginal caspase-3 immunoreactivity with very few sub-G1 apoptotic cell proportions in flow cytometry. In addition, the suppression of caspase-3 or Bax action failed to prevent cell death of primary cortical neurons, indicating minimal apoptotic cell death. On the other hand, there was a marked increase in the TdT-mediated dUTP nick end labeling-positive and propidium iodide-labeled necrotic cells (∼50%) with enhanced poly [ADP-ribose] polymerase-1 activity. Therefore, prevention against necrosis rather than apoptosis may be required for optimal benefits in cell transplantation.


Subject(s)
Cell Death/physiology , Neurons/cytology , Animals , Blotting, Western , Cell Survival/physiology , Cell Transplantation , Cerebral Cortex/cytology , Cerebral Cortex/transplantation , Embryo, Mammalian , Immunohistochemistry , In Situ Nick-End Labeling , Neurons/transplantation , Rats , Rats, Sprague-Dawley
7.
Neuron ; 73(5): 911-24, 2012 Mar 08.
Article in English | MEDLINE | ID: mdl-22405202

ABSTRACT

The positioning of neurons in the cerebral cortex is of crucial importance for its function as highlighted by the severe consequences of migrational disorders in patients. Here we show that genetic deletion of the small GTPase RhoA in the developing cerebral cortex results in two migrational disorders: subcortical band heterotopia (SBH), a heterotopic cortex underlying the normotopic cortex, and cobblestone lissencephaly, in which neurons protrude beyond layer I at the pial surface of the brain. Surprisingly, RhoA(-/-) neurons migrated normally when transplanted into wild-type cerebral cortex, whereas the converse was not the case. Alterations in the radial glia scaffold are demonstrated to cause these migrational defects through destabilization of both the actin and the microtubules cytoskeleton. These data not only demonstrate that RhoA is largely dispensable for migration in neurons but also showed that defects in radial glial cells, rather than neurons, can be sufficient to produce SBH.


Subject(s)
Cerebral Cortex/cytology , Cerebral Cortex/physiology , Neuroglia/metabolism , Neurons/physiology , rhoA GTP-Binding Protein/deficiency , Age Factors , Animals , Animals, Newborn , Bromodeoxyuridine/metabolism , Cell Movement , Cell Proliferation , Cerebral Cortex/embryology , Cerebral Cortex/transplantation , Classical Lissencephalies and Subcortical Band Heterotopias/genetics , Classical Lissencephalies and Subcortical Band Heterotopias/metabolism , Classical Lissencephalies and Subcortical Band Heterotopias/pathology , Disease Models, Animal , Electroporation , Embryo, Mammalian , Embryonic Stem Cells/physiology , Embryonic Stem Cells/transplantation , Female , GAP-43 Protein/genetics , GAP-43 Protein/metabolism , Gene Expression Regulation, Developmental/genetics , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , In Vitro Techniques , Mice , Mice, Transgenic , Nerve Tissue Proteins/metabolism , Neuroglia/physiology , Neurons/metabolism , Neurons/ultrastructure , Pregnancy , Silver Staining , rhoA GTP-Binding Protein/genetics
8.
Neuroscience ; 201: 357-65, 2012 Jan 10.
Article in English | MEDLINE | ID: mdl-22079578

ABSTRACT

GABAergic interneurons are local circuit cells that control the excitatory balance in most regions of the nervous system, particularly the cerebral cortex. Because they are integrated in every cortical module, we posed the question whether interneuronal precursors would display some topographic specificity between their origin at the ventral telencephalon and their cortical location after migration. If this was true, GABAergic cells would have to be provided with intrinsic features that would make them able to perform specific functional roles in each specific module. On the other hand, if no topography was found, one would conclude that inhibitory precursors would be functionally naive, being able to integrate anywhere in the cortex, with equal capacity of performing their functions. This issue was approached by use of organotypic cultures of wild mice embryonic slices, into which fragments of the ganglionic eminence taken from enhanced green fluorescent protein (eGFP) mice were implanted, observing the topographic location of both the implant and its destination. Despite the existence of different genetic domains in the ventricular zone of the medial ganglionic eminences (MGE), we found that cells originating in different regions spread in vitro all over the mediolateral axis of the developing cortical wall, independently of their sites of origin. Results favor the hypothesis that GABAergic precursors are functionally naive, integrating into modules irrespective of which cortical area they belong to.


Subject(s)
Cerebral Cortex/cytology , Cerebral Cortex/embryology , Gene Expression Regulation, Developmental , Interneurons/physiology , Age Factors , Animals , Cell Movement/physiology , Cerebral Cortex/transplantation , Cerebral Ventricles/cytology , Cerebral Ventricles/embryology , Coculture Techniques , Embryo, Mammalian , Female , Green Fluorescent Proteins/genetics , Male , Mice , Mice, Transgenic , Organ Culture Techniques , Pregnancy , Time Factors , Transcription Factors/genetics , Transcription Factors/metabolism , gamma-Aminobutyric Acid/metabolism
9.
J Neuroinflammation ; 8: 122, 2011 Sep 26.
Article in English | MEDLINE | ID: mdl-21942980

ABSTRACT

BACKGROUND: While it is clear that inbred strains of mice have variations in immunological responsiveness, the influence of genetic background following tissue damage in the central nervous system is not fully understood. A cortical explant system was employed as a model for injury to determine whether the immediate transcriptional response to tissue resection revealed differences among three mouse strains. METHODS: Immunological mRNAs were measured in cerebral cortex from SJL/J, C57BL/6J, and BALB/cJ mice using real time RT-PCR. Freshly isolated cortical tissue and cortical sections incubated in explant medium were examined. Levels of mRNA, normalized to ß-actin, were compared using one way analysis of variance with pooled samples from each mouse strain. RESULTS: In freshly isolated cerebral cortex, transcript levels of many pro-inflammatory mediators were not significantly different among the strains or too low for comparison. Constitutive, baseline amounts of CD74 and antisecretory factor (ASF) mRNAs, however, were higher in SJL/J and C57BL/6J, respectively. When sections of cortical tissue were incubated in explant medium, increased message for a number of pro-inflammatory cytokines and chemokines occurred within five hours. Message for chemokines, IL-1α, and COX-2 transcripts were higher in C57BL/6J cortical explants relative to SJL/J and BALB/cJ. IL-1ß, IL-12/23 p40, and TNF-α were lower in BALB/cJ explants relative to SJL/J and C57BL/6J. Similar to observations in freshly isolated cortex, CD74 mRNA remained higher in SJL/J explants. The ASF mRNA in SJL/J explants, however, was now lower than levels in both C57BL/6J and BALB/cJ explants. CONCLUSIONS: The short-term cortical explant model employed in this study provides a basic approach to evaluate an early transcriptional response to neurological damage, and can identify expression differences in genes that are influenced by genetic background.


Subject(s)
Brain Tissue Transplantation/methods , Central Nervous System/injuries , Cerebral Cortex/transplantation , Mice, Inbred Strains , Transcription, Genetic , Animals , Chemokines/genetics , Chemokines/metabolism , Cytokines/genetics , Cytokines/metabolism , Humans , Mice , RNA, Messenger/metabolism
10.
Cereb Cortex ; 20(10): 2348-60, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20100897

ABSTRACT

During cerebral cortex development, different cell populations migrate tangentially through the preplate, traveling from their site of origin toward their final positions. One of the earliest populations formed, the Cajal-Retzius (C-R) cells, is mainly generated in different cortical hem (CH) domains, and they migrate along established and parallel routes to cover the whole cortical mantle. In this study, we present evidence that the phenotype of -Retzius cells, as well as some of their migratory characteristics, is specified in the area where the cells are generated. Nevertheless, when implanted ectopically, these cells can follow new migratory routes, indicating that locally provided genetic cues along the migratory path nonautonomously influence the position of these cells emanating from different portions of the CH. This was witnessed by performing CH implants of tissue expressing fluorescent tracers in live whole embryos. In the same way, tracer injections into the hem of Small eye mutant mice were particularly informative since the lack of Pax6 affects some guidance factors in the migratory environment. As a result, in these animals, the C-R cell population is disorganized, and it forms 1 day late, showing certain differences in gene expression that might help explain these disruptions.


Subject(s)
Cell Movement/physiology , Cerebral Cortex/cytology , Cerebral Cortex/embryology , Gene Expression Regulation, Developmental/physiology , Neurons/physiology , Animals , Benzopyrans/metabolism , Brain Tissue Transplantation/methods , Cell Differentiation/physiology , Cell Movement/genetics , Cerebral Cortex/transplantation , Embryo, Mammalian , Eye Proteins/genetics , Female , Fluoresceins/metabolism , Green Fluorescent Proteins/genetics , Homeodomain Proteins/genetics , In Vitro Techniques , Mice , Mice, Inbred C57BL , Mice, Transgenic , Mutation/genetics , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Neurons/classification , PAX6 Transcription Factor , Paired Box Transcription Factors/genetics , Pregnancy , Repressor Proteins/genetics
11.
Neurotoxicol Teratol ; 32(1): 109-13, 2010.
Article in English | MEDLINE | ID: mdl-19217940

ABSTRACT

The identification of mechanisms and outcomes for neurobehavioral teratogenesis is critical to our ability to develop therapies to ameliorate or reverse the deleterious effects of exposure to developmental neurotoxicants. We established mechanistically-based complementary models for the study of cholinergic systems in the mouse and the chick, using both environmental neurotoxicants (chlorpyrifos, perfluoroalkyls) and drugs of abuse (heroin, nicotine, PCP). Behavioral evaluations were made using the Morris maze in the mouse, evaluating visuospatial memory related to hippocampal cholinergic systems, and imprinting in the chick, examining behavior dependent on cholinergic innervation of the IMHV. In both models we demonstrated the dependence of neurobehavioral deficits on impairment of cholinergic receptor-induced expression, and translocation of specific PKC isoforms. Understanding this mechanism, we were able to reverse both the synaptic and behavioral deficits with administration of neural progenitors. We discuss the prospects for clinical application of neural progenitor therapy, emphasizing protocols for reducing or eliminating immunologic rejection, as well as minimizing invasiveness of procedures through development of intravenous administration protocols.


Subject(s)
Abnormalities, Drug-Induced/therapy , Cerebral Cortex/transplantation , Fluorocarbons/toxicity , Illicit Drugs/toxicity , Neurons/transplantation , Stem Cell Transplantation/methods , Abnormalities, Drug-Induced/psychology , Animals , Brain Tissue Transplantation/methods , Chickens , Cholinesterase Inhibitors/toxicity , Female , Fetal Tissue Transplantation/methods , Imprinting, Psychological/drug effects , Maze Learning/drug effects , Mice , Stem Cell Transplantation/psychology , Teratogens , Toxicity Tests/methods
12.
BMC Neurosci ; 10: 86, 2009 Jul 21.
Article in English | MEDLINE | ID: mdl-19622162

ABSTRACT

BACKGROUND: The human neural stem cell line CTX0E03 was developed for the cell based treatment of chronic stroke disability. Derived from fetal cortical brain tissue, CTX0E03 is a clonal cell line that contains a single copy of the c-mycERTAM transgene delivered by retroviral infection. Under the conditional regulation by 4-hydroxytamoxifen (4-OHT), c-mycERTAM enabled large-scale stable banking of the CTX0E03 cells. In this study, we investigated the fate of this transgene following growth arrest (EGF, bFGF and 4-OHT withdrawal) in vitro and following intracerebral implantation into a mid-cerebral artery occluded (MCAo) rat brain. In vitro, 4-weeks after removing growth factors and 4-OHT from the culture medium, c-mycERTAM transgene transcription is reduced by ~75%. Furthermore, immunocytochemistry and western blotting demonstrated a concurrent decrease in the c-MycERTAM protein. To examine the transcription of the transgene in vivo, CTX0E03 cells (450,000) were implanted 4-weeks post MCAo lesion and analysed for human cell survival and c-mycERTAM transcription by qPCR and qRT-PCR, respectively. RESULTS: The results show that CTX0E03 cells were present in all grafted animal brains ranging from 6.3% to 39.8% of the total cells injected. Prior to implantation, the CTX0E03 cell suspension contained 215.7 (SEM = 13.2) copies of the c-mycERTAM transcript per cell. After implantation the c-mycERTAM transcript copy number per CTX0E03 cell had reduced to 6.9 (SEM = 3.4) at 1-week and 7.7 (SEM = 2.5) at 4-weeks. Bisulfite genomic DNA sequencing of the in vivo samples confirmed c-mycERTAM silencing occurred through methylation of the transgene promoter sequence. CONCLUSION: In conclusion the results confirm that CTX0E03 cells downregulated c-mycERTAM transgene expression both in vitro following EGF, bFGF and 4-OHT withdrawal and in vivo following implantation in MCAo rat brain. The silencing of the c-mycERTAM transgene in vivo provides an additional safety feature of CTX0E03 cells for potential clinical application.


Subject(s)
Cerebral Cortex/transplantation , Fetal Stem Cells/transplantation , Gene Silencing , Infarction, Middle Cerebral Artery/genetics , Animals , Cell Line , Cells, Cultured , Cerebral Cortex/blood supply , Fetal Stem Cells/cytology , Humans , Male , Neurons/cytology , Rats , Rats, Sprague-Dawley , Transgenes , Transplantation, Heterologous
13.
Brain Dev ; 31(10): 717-24, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19118961

ABSTRACT

We performed a cell transplantation study to treat the brain involvement in lysosomal storage diseases. We used acid beta-galactosidase knock-out mice (BKO) from C57BL/6 as recipients. To minimize immune responses, we used cells derived from transgenic mice of C57BL/6 overexpressing the normal human beta-galactosidase. Fetal brain cells (FBC), bone marrow-derived mesenchymal stem cells (MSC), and mixed FBC and MSC cells were prepared and injected into the ventricle of newborn BKO mouse brain. The mice were examined at 1, 2, 4, and 8 weeks and 6 months after injection. In each experiment, the injected cells migrated into the whole brain effectively and survived for at least 8 weeks. Decrease in ganglioside GM1 level was also observed. FBC could survive for 6 months in recipient brain. However, the number of transplanted FBC decreased. In the brains of MSC- or mixed cell-treated mice, no grafted cells could be found at 6 months. To achieve sufficient long-term effects on the brain, a method of steering the immune response away from cytotoxic responses or of inducing tolerance to the products of therapeutic genes must be developed.


Subject(s)
Cerebral Cortex/transplantation , Gangliosidosis, GM1/therapy , Mesenchymal Stem Cell Transplantation , Animals , Blotting, Western , Brain Tissue Transplantation , Cells, Cultured , Fetal Tissue Transplantation , Gangliosidosis, GM1/genetics , Humans , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , beta-Galactosidase/genetics , beta-Galactosidase/metabolism
14.
Neuropathology ; 29(4): 410-21, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19170896

ABSTRACT

Transplantation of stem cells is a potential therapeutic strategy for stroke damage. The survival, migration, and differentiation of transplanted human embryonic neural stem cells in the acute post-ischemic environment were characterized and endogenous nestin expression after transplantation was investigated. Human embryonic neural stem cells obtained from the temporal lobe cortex were cultured and labeled with fluorescent 1,1'-dioctadecy-6,6'-di (4-sulfopheyl)-3,3,3',3'-tetramethylindocarbocyanin (DiI) in vitro. Labeled cells were transplanted into cortical peri-infarction zones of adult rats 24 h after permanent middle cerebral artery occlusion. Survival, migration, and differentiation of grafted cells were quantified in immunofluorescence-stained sections from rats sacrificed at 7, 14, and 28 days after transplantation. Endogenous nestin-positive cells in the cortical peri-infarction zone were counted at serial time points. The cells transplanted into the cortical peri-infarction zone displayed the morphology of living cells and became widely located around the ischemic area. Moreover, some of the transplanted cells expressed nestin, GFAP, or NeuN in the peri-infarction zone. Furthermore, compared with the control group, endogenous nestin-positive cells in the peri-infarction zone had increased significantly 7 days after cell transplantation. These results confirm the survival, migration, and differentiation of transplanted cells in the acute post-ischemic environment and enhanced endogenous nestin expression within a brief time window. These findings indicate that transplantation of neural stem cells into the peri-infarction zone may be performed as early as 24 h after ischemia.


Subject(s)
Cell Differentiation/physiology , Cell Movement/physiology , Cerebral Cortex/transplantation , Cerebral Infarction/metabolism , Cerebral Infarction/pathology , Embryonic Stem Cells/transplantation , Gene Expression Regulation, Developmental/physiology , Intermediate Filament Proteins/biosynthesis , Nerve Tissue Proteins/biosynthesis , Age Factors , Animals , Cell Survival/physiology , Cells, Cultured , Cerebral Cortex/cytology , Cerebral Cortex/metabolism , Cerebral Infarction/surgery , Embryonic Stem Cells/cytology , Embryonic Stem Cells/physiology , Humans , Male , Nestin , Rats , Rats, Sprague-Dawley , Stem Cell Transplantation/methods , Time Factors
15.
Neural Plast ; 2009: 852492, 2009.
Article in English | MEDLINE | ID: mdl-20169102

ABSTRACT

Neural transplantation has emerged as an attractive strategy for the replacement of neurons that have been lost in the central nervous system. Multipotent neural progenitor cells are potentially useful as donor cells to repopulate the degenerated regions. One important aspect of a transplantation strategy is whether transplanted cells are capable of fiber outgrowth with the aim of rebuilding axonal connections within the host brain. To address this issue, we expanded neuronal progenitor from the cortex of embryonic day 15 ubiquitously green fluorescent protein-expressing transgenic mice as neurospheres in vitro and grafted them into the entorhinal cortex of 8-week-old mice immediately after a perforant pathway lesion. After transplantation into a host brain with a lesion of the entorhino-hippocampal projection, the neurosphere-derived cells extended long fiber projections directed towards the dentate gyrus. Our results indicate that transplantation of neurosphere-derived cells might be a promising strategy to replace lost or damaged axonal projections.


Subject(s)
Brain Tissue Transplantation , Brain/physiopathology , Brain/surgery , Cerebral Cortex/transplantation , Embryonic Stem Cells/transplantation , Fetal Tissue Transplantation , Neurons/transplantation , Aging , Animals , Astrocytes/physiology , Brain Injuries/physiopathology , Brain Injuries/surgery , Cell Differentiation , Cerebral Cortex/embryology , Dentate Gyrus/physiopathology , Embryonic Stem Cells/physiology , Entorhinal Cortex/physiopathology , Entorhinal Cortex/surgery , Female , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Mice , Mice, Inbred Strains , Mice, Transgenic , Neural Pathways/injuries , Neural Pathways/physiopathology , Neural Pathways/surgery , Neurogenesis , Neurons/physiology , Perforant Pathway/injuries , Perforant Pathway/physiopathology
16.
J Neurophysiol ; 100(2): 839-51, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18509082

ABSTRACT

Unlike adult spinalized rats, approximately 20% of rats spinalized as postnatal day 1 or 2 (P1/P2) neonates achieve autonomous hindlimb weight support. Cortical representations of mid/low trunk occur only in such rats with high weight support. However, the importance of hindlimb/trunk motor cortex in function of spinalized rats remains unclear. We tested the importance of trunk sensorimotor cortex in their locomotion using lesions guided by cortical microstimulation in P1/P2 weight-supporting neonatal spinalized rats and controls. In four intact control rats, lesions of hindlimb/trunk cortex caused no treadmill deficits. All spinalized rats lesioned in trunk cortex (n = 16: 4 transplant, 6 transect, 6 transect + fibrin glue) lost an average of about 40% of their weight support. Intact trunk cortex was essential to their level of function. Lesion of trunk cortex substantially increased roll of the hindquarters, which correlated to diminished weight support, but other kinematic stepping parameters showed little change. Embryonic day 14 (E14) transplants support development of the trunk motor representations in their normal location. We tested the role of novel relay circuits arising from the grafts in such cortical representations in E14 transplants using the rats that received (noncellular) fibrin glue grafting at P1/P2 (8 allografts and 32 xenografts). Fibrin-repaired rats with autonomous weight support also had trunk cortical representations similar to those of E14 transplant rats. Thus acellular repair and intrinsic plasticity were sufficient to support the observed features. Our data show that effective cortical mechanisms for trunk control are essential for autonomous weight support in P1/P2 spinalized rats and these can be achieved by intrinsic plasticity.


Subject(s)
Cerebral Cortex/physiopathology , Hindlimb/innervation , Locomotion/physiology , Spinal Cord Injuries/physiopathology , Weight-Bearing/physiology , Animals , Animals, Newborn , Biomechanical Phenomena , Brain Mapping , Cerebral Cortex/injuries , Cerebral Cortex/transplantation , Electric Stimulation/methods , Embryo, Mammalian , Exercise Test , Fetal Tissue Transplantation/methods , Fibrin Tissue Adhesive/therapeutic use , Hindlimb/physiopathology , Linear Models , Rats , Rats, Sprague-Dawley , Spinal Cord/transplantation , Spinal Cord Injuries/therapy , Tissue Adhesives/therapeutic use
18.
Eur J Neurosci ; 26(3): 605-14, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17686040

ABSTRACT

Stroke is a neurodegenerative disorder and the leading cause of disability in adult humans. Treatments to support efficient recovery in stroke patients are lacking. Several studies have demonstrated the ability of grafted neural stem cells (NSCs) to partly improve impaired neurological functions in stroke-subjected animals. Recently, we reported that NSCs from human fetal striatum and cortex exhibit region-specific differentiation in vitro, but survive, migrate and form neurons to a similar extent after intrastriatal transplantation in newborn rats. Here, we have transplanted the same cells into the stroke-damaged striatum of adult rats. The two types of NSCs exhibited a similar robust survival (30%) at 1 month after transplantation, and migrated throughout the damaged striatum. Striatal NSCs migrated farther and occupied a larger volume of striatum. In the transplantation core, cells were undifferentiated and expressed nestin and, to a lesser extent, also GFAP, betaIII-tubulin, DCX and calretinin, markers of immature neural lineage. Immunocytochemistry using markers of proliferation (p-H3 and Ki67) revealed a very low content of proliferating cells (<1%) in the grafts. Human cells outside the transplantation core differentiated, exhibited mature neuronal morphology and expressed mature neuronal markers such as HuD, calbindin and parvalbumin. Interestingly, striatal NSCs generated a greater number of parvalbumin+ and calbindin+ neurons. Virtually none of the grafted cells differentiated into astrocytes or oligodendrocytes. Based on these data, human fetal striatum- and cortex-derived NSCs could be considered potentially safe and viable for transplantation, with strong neurogenic potential, for further exploration in animal models of stroke.


Subject(s)
Brain Tissue Transplantation/methods , Graft Survival/physiology , Neurons/physiology , Stem Cell Transplantation/methods , Stem Cells/physiology , Stroke/therapy , Animals , Biomarkers/metabolism , Brain Tissue Transplantation/trends , Cell Differentiation/physiology , Cell Movement/physiology , Cell Proliferation , Cell Survival/physiology , Cells, Cultured , Cerebral Cortex/cytology , Cerebral Cortex/physiology , Cerebral Cortex/transplantation , Corpus Striatum/cytology , Corpus Striatum/physiology , Corpus Striatum/transplantation , Disease Models, Animal , Doublecortin Protein , Humans , Infarction, Middle Cerebral Artery/pathology , Infarction, Middle Cerebral Artery/physiopathology , Infarction, Middle Cerebral Artery/therapy , Male , Nerve Tissue Proteins/metabolism , Neurons/cytology , Rats , Rats, Wistar , Stem Cell Transplantation/trends , Stem Cells/cytology , Stroke/pathology , Stroke/physiopathology , Transplantation, Heterologous
19.
Proc Natl Acad Sci U S A ; 104(8): 2956-60, 2007 Feb 20.
Article in English | MEDLINE | ID: mdl-17301224

ABSTRACT

It is known that Alzheimer's disease (AD) is a synaptic disease that involves various neurotransmitter systems, particularly those where synaptic transmission is mediated by acetylcholine or glutamate (Glu). Nevertheless, very little is known about the properties of neurotransmitter receptors of the AD human brain. We have shown previously that cell membranes, carrying neurotransmitter receptors from the human postmortem brain, can be transplanted to frog oocytes, and their receptors will still be functional. Taking advantage of this fact, we have now studied the properties of Glu receptors (GluRs) from the cerebral cortices of AD and non-AD brains and found that oocytes injected with AD membranes acquired GluRs that have essentially the same functional properties as those of oocytes injected with membranes from non-AD brains. However, the amplitudes of the currents elicited by Glu were always smaller in the oocytes injected with membranes from AD brains. Western blot analyses of the same membrane preparations used for the electrophysiological studies showed that AD membranes contained significantly fewer GluR2/3 subunit proteins. Furthermore, the corresponding mRNAs were also diminished in the AD brain. Therefore, the smaller amplitude of membrane currents elicited by Glu in oocytes injected with membranes from an AD brain is a consequence of a reduced number of GluRs in cell membranes transplanted from the AD brain. Thus, using the comparatively simple method of microtransplantation of receptors, it is now possible to determine the properties of neurotransmitter receptors of normal and diseased human brains. That knowledge may help to decipher the etiology of the diseases and also to develop new treatments.


Subject(s)
Alzheimer Disease/pathology , Anura/metabolism , Brain Tissue Transplantation , Cerebral Cortex/metabolism , Cerebral Cortex/transplantation , Oocytes/metabolism , Receptors, Glutamate/metabolism , Animals , Benzothiadiazines/pharmacology , Blotting, Western , Cell Membrane/drug effects , Electric Conductivity , Gene Expression Regulation/drug effects , Glutamic Acid/pharmacology , Humans , Kainic Acid/pharmacology , Oocytes/drug effects , Protein Subunits/genetics , Protein Subunits/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, GABA/genetics , Receptors, GABA/metabolism , Receptors, Glutamate/genetics , Reverse Transcriptase Polymerase Chain Reaction , Time Factors , gamma-Aminobutyric Acid/pharmacology
20.
Neuroscience ; 128(4): 807-17, 2004.
Article in English | MEDLINE | ID: mdl-15464288

ABSTRACT

The subventricular zone (SVZ) in the forebrain is the largest source of neural stem cells and progenitor cells in the adult CNS. To assess the ability of adult neural stem cells to survive, differentiate and migrate, we have compared the behavior of dissociated, neurosphere-derived stem cells with that of progenitor cells in transplantation experiments. This ability was first tested in vivo, offering the stem cells the possibility to migrate along the rostral migratory stream (RMS), their specific pathway. In addition, the differential behaviors of the two classes of cells were also compared in vitro by grafting them into organotypic slice cultures containing either tangential (embryonic cerebral cortex) or radial (early postnatal cerebellar cortex) migratory routes. Most of the grafted adult neurosphere-derived stem cells survived and integrated in vivo, and a proportion of them differentiate into neurons, oligodendrocytes or astrocytes. However, they were unable to migrate along the RMS and remained in the vicinity of the injection site. In contrast, SVZ progenitor cells were able to migrate toward the olfactory bulb and, once there, to acquire the phenotype of granule cells, as previously reported. In vitro, neural stem cells exhibited a better migratory ability, although they only migrated for short distances, particularly, in forebrain slices. Nevertheless, the average distance covered by progenitor cells was a two-fold longer than that covered by neural stem cells, corroborating that this class of more specified cells has higher migratory ability. These results suggest that the in vitro conditions of expanding SVZ-derived stem cells, required to maintain them in an immature stage might modify their intrinsic properties, preventing their differentiation into neuroblasts and their subsequent migration.


Subject(s)
Cell Movement/physiology , Cerebral Ventricles/cytology , Neurons/physiology , Stem Cells/cytology , Analysis of Variance , Animals , Animals, Newborn , Cell Count/methods , Cells, Cultured , Cerebral Cortex/growth & development , Cerebral Cortex/transplantation , Cerebral Ventricles/growth & development , Embryo, Mammalian , Glial Fibrillary Acidic Protein/metabolism , Green Fluorescent Proteins/metabolism , Immunohistochemistry/methods , Mice , Myelin Proteolipid Protein/metabolism , Neuroglia/physiology , Organ Culture Techniques/methods , Phosphopyruvate Hydratase/metabolism , Prosencephalon/embryology , Prosencephalon/metabolism , Stem Cell Transplantation/methods , Stem Cells/physiology , Time Factors , Transplants , Tubulin/metabolism
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