Evaluation of Muscle Chemical and Amino Acids Composition in Broiler Chicks Fed Sorghum or Sorghum-Pea Diets

The study evaluated the chemical and amino acids (AA) composition of breast and thigh muscle in broilers fed sorghum and sorghum-pea diets, as partial substitute of corn and soybean meal (SBM). A total of 540 3-wk-old broilers (Cobb 500) randomly assigned to three groups were fed with corn-SBM control diet (C), corn-sorghum-SBM diet (S) and corn-sorghum-peas-SBM diet (SP) for finisher phase. At slaughter, muscle samples were collected for chemical analyses. The results showed that dietary sorghum or sorghum-pea inclusion did not affect (p>0.05) the chemical composition (dry matter, protein, fat and ash) of broilers muscle tissue. The total AA (TAA), essential AA (EAA) or flavour-related AA (FAA) concentrations from breast or thigh muscle did not differ (p>0.05) between treatments. A significant effect (p<0.05) was found for some individual EAA. The valine and phenylalanine concentrations were higher, and cysteine and methionine levels were lower in both muscles than the C group. The higher deposition (p<0.05) was found for most AA, except glycine and arginine, in the breast vs thigh, as an effect of muscle tissue. As results, the TAA, EAA, NEAA, FAA and EAA/NEAA ratio increased in the breast vs thigh muscle. Interactions between diets and muscle tissue were noticed for serine, threonine, methionine, isoleucine, phenylalanine, lysine, arginine, TAA and EAA. In conclusion, sorghum or sorghum-peas can partially substitute the corn and SBM in broiler diets, with no adverse effects on chemical composition and beneficial nutrients, such as EAA and FAA that are important for the nutritional quality of meat.(AU)

Evaluation of Muscle Chemical and Amino Acids Composition in Broiler Chicks Fed Sorghum or Sorghum-Pea Diets

The study evaluated the chemical and amino acids (AA) composition of breast and thigh muscle in broilers fed sorghum and sorghum-pea diets, as partial substitute of corn and soybean meal (SBM). A total of 540 3-wk-old broilers (Cobb 500) randomly assigned to three groups were fed with corn-SBM control diet (C), corn-sorghum-SBM diet (S) and corn-sorghum-peas-SBM diet (SP) for finisher phase. At slaughter, muscle samples were collected for chemical analyses. The results showed that dietary sorghum or sorghum-pea inclusion did not affect (p>0.05) the chemical composition (dry matter, protein, fat and ash) of broilers muscle tissue. The total AA (TAA), essential AA (EAA) or flavour-related AA (FAA) concentrations from breast or thigh muscle did not differ (p>0.05) between treatments. A significant effect (p<0.05) was found for some individual EAA. The valine and phenylalanine concentrations were higher, and cysteine and methionine levels were lower in both muscles than the C group. The higher deposition (p<0.05) was found for most AA, except glycine and arginine, in the breast vs thigh, as an effect of muscle tissue. As results, the TAA, EAA, NEAA, FAA and EAA/NEAA ratio increased in the breast vs thigh muscle. Interactions between diets and muscle tissue were noticed for serine, threonine, methionine, isoleucine, phenylalanine, lysine, arginine, TAA and EAA. In conclusion, sorghum or sorghum-peas can partially substitute the corn and SBM in broiler diets, with no adverse effects on chemical composition and beneficial nutrients, such as EAA and FAA that are important for the nutritional quality of meat.

Physicochemical characterization of by-products from beef cattle slaughter and economic feasibility of commercialization

The aim was to evaluate the proximate food composition, cholesterol content, coloring and economic aspects of the main nutrients present in by-products from the slaughter of cattle. The samples were collected from 14 organs, with four repetitions each, of which we conducted the analyses of moisture, crude protein, mineral matter, cholesterol and fat content. The color was measured through the coordinates L*, a*, b*. The differences between the groups of red and white viscera were evaluated by contrasts analysis. Subsequently, the data were subjected to multivariate analysis of variance, which was complemented by the principal component technique. In the analysis of contrast between red and white viscera, the results showed that the components with the highest content of total fat does not necessarily have the highest content of cholesterol and vice-versa. For minerals, the red viscera presented higher content than white viscera and, in order to analyze the protein composition, there was less variability for red viscera in relation to white viscera. In the color analysis, the values for the color readings indicated uniformity between the external and internal readings, except for the tongue. On mean, the proximate composition values for the offals are similar to the values for beef, especially regarding the red viscera.

Physicochemical characterization of by-products from beef cattle slaughter and economic feasibility of commercialization

The aim was to evaluate the proximate food composition, cholesterol content, coloring and economic aspects of the main nutrients present in by-products from the slaughter of cattle. The samples were collected from 14 organs, with four repetitions each, of which we conducted the analyses of moisture, crude protein, mineral matter, cholesterol and fat content. The color was measured through the coordinates L*, a*, b*. The differences between the groups of red and white viscera were evaluated by contrasts analysis. Subsequently, the data were subjected to multivariate analysis of variance, which was complemented by the principal component technique. In the analysis of contrast between red and white viscera, the results showed that the components with the highest content of total fat does not necessarily have the highest content of cholesterol and vice-versa. For minerals, the red viscera presented higher content than white viscera and, in order to analyze the protein composition, there was less variability for red viscera in relation to white viscera. In the color analysis, the values for the color readings indicated uniformity between the external and internal readings, except for the tongue. On mean, the proximate composition values for the offals are similar to the values for beef, especially regarding the red viscera.(AU)

Comparison of Physicochemical and Functional Properties of Chicken and Duck Egg Albumens

The aim of this research was to investigate the physicochemical and functional properties of fresh and pasteurized chicken and duck egg albumens. The results showed that pasteurization of both chicken and duck albumens significantly decreased (p0.05) viscosity, but had no impact (p>0.05) on pH or free sulfhydryl groups. Chicken albumen was shown to have higher (p0.05) foam expansion, but lower (p0.05) foam stability than duck albumen. Pasteurization decreased (p0.05) the foam expansion of both albumens while decreasing (p0.05) the foam stability only of duck albumen. Investigation of the gel properties showed that duck albumen has greater hardness and lower expressible water (p0.05) than chicken albumen. Pasteurization increased the hardness and decreased the expressible water of both the chicken and duck albumen gels. This study suggests that the superior gel properties of duck albumen offer potential approaches to improving the quality of gel food products.(AU)

Digestibility and Physicochemical Characteristics of Tambaqui Waste Biological Silage Meal Included in Commercial Layer Diets*

This study aimed at evaluating the effects of the physicochemical and nutritional characteristics of a biological silage meal made of tambaqui waste on dietary nutrient apparent digestibility and energy metabolism of commercial layer diets. Seventy-two 61-week-old Hisex White hens were assigned according to a completely randomized experimental design in two treatments: control diet and diet with 5% tambaqui waste biological silage meal (TWBSM), with six replicates of six birds each. The ensiled biomass presented grey brown color, acid aroma, viscous texture, 5.27±0.016pH, 96.28% dry matter (DM), 33.29% crude protein (CP), 33.65% ether extract (EE), 14.37% ashes, 13.63% non-nitrogen extract (NNE), 65.16 g.kg-1 calcium (Ca), 22.90 g.kg-1 phosphorus (P), and 5,666.07 kcal.kg-1 gross energy (GE). Higher (p>0.05) coefficients of apparent digestibility of DM, crude fiber, EE, and ash, and higher coefficient of GE metabolizability were obtained in the diet with 5% TWBSM. Due to its high CP content (33.29% on DM basis), TWBSM may be considered a protein feedstuff. Although no differences in CP digestibility (p>0.05) were detected, TWBSM presented high CP digestibility than the control diet due to proteins hydrolysis as a result of lactic fermentation by microorganisms during the ensilage process, yielding autolysed proteins of high biological value. Our results indicate that the biological silage meal produced from the residual biomass of tambaqui can be included up to 5% in commercial layer diets as it has good nutrient digestibility, and therefore, may potentially be used as a dietary energy and protein source.(AU)

Comparison of Physicochemical and Functional Properties of Chicken and Duck Egg Albumens

The aim of this research was to investigate the physicochemical and functional properties of fresh and pasteurized chicken and duck egg albumens. The results showed that pasteurization of both chicken and duck albumens significantly decreased (p0.05) viscosity, but had no impact (p>0.05) on pH or free sulfhydryl groups. Chicken albumen was shown to have higher (p0.05) foam expansion, but lower (p0.05) foam stability than duck albumen. Pasteurization decreased (p0.05) the foam expansion of both albumens while decreasing (p0.05) the foam stability only of duck albumen. Investigation of the gel properties showed that duck albumen has greater hardness and lower expressible water (p0.05) than chicken albumen. Pasteurization increased the hardness and decreased the expressible water of both the chicken and duck albumen gels. This study suggests that the superior gel properties of duck albumen offer potential approaches to improving the quality of gel food products.

Digestibility and Physicochemical Characteristics of Tambaqui Waste Biological Silage Meal Included in Commercial Layer Diets*

This study aimed at evaluating the effects of the physicochemical and nutritional characteristics of a biological silage meal made of tambaqui waste on dietary nutrient apparent digestibility and energy metabolism of commercial layer diets. Seventy-two 61-week-old Hisex White hens were assigned according to a completely randomized experimental design in two treatments: control diet and diet with 5% tambaqui waste biological silage meal (TWBSM), with six replicates of six birds each. The ensiled biomass presented grey brown color, acid aroma, viscous texture, 5.27±0.016pH, 96.28% dry matter (DM), 33.29% crude protein (CP), 33.65% ether extract (EE), 14.37% ashes, 13.63% non-nitrogen extract (NNE), 65.16 g.kg-1 calcium (Ca), 22.90 g.kg-1 phosphorus (P), and 5,666.07 kcal.kg-1 gross energy (GE). Higher (p>0.05) coefficients of apparent digestibility of DM, crude fiber, EE, and ash, and higher coefficient of GE metabolizability were obtained in the diet with 5% TWBSM. Due to its high CP content (33.29% on DM basis), TWBSM may be considered a protein feedstuff. Although no differences in CP digestibility (p>0.05) were detected, TWBSM presented high CP digestibility than the control diet due to proteins hydrolysis as a result of lactic fermentation by microorganisms during the ensilage process, yielding autolysed proteins of high biological value. Our results indicate that the biological silage meal produced from the residual biomass of tambaqui can be included up to 5% in commercial layer diets as it has good nutrient digestibility, and therefore, may potentially be used as a dietary energy and protein source.

Agricultural answers and chemical composition of Massai grass under different nitrogen doses and urea sources / Respostas agronômicas e de composição química do capim Massai sob diferentes doses de nitrogênio e fontes de ureia

Under the hypothesis that modifying nitrogen sources and doses could increase nitrogen fertilization efficiency and productivity and improve forage quality, this study aimed to evaluate the morphological composition, structural characteristics and chemical composition of Panicum maximum cv. Massai forage cultivated under different urea sources (common and coated with Policote®) and nitrogen doses (200, 400 and 600 kg ha-1 year-1) during the autumn, winter and spring of 2014 and the summer and autumn of 2015. The experiment was carried out in Seropédica, RJ, under a randomized complete block design in a factorial arrangement (3x2) + 1 with four replications. High nitrogen rates promoted higher percentages of leaf blade dry mass and lower percentages of dead material dry mass in the forage mass and provided higher tiller population density and forage accumulation rate of Massai grass during the studied seasons. The use of coated urea promoted higher levels of crude protein in the forage than did the use of common urea in all seasons. The intensification of nitrogen fertilization reduced the nitrogen use efficiency but benefitted the structural characteristics, forage accumulation and chemical composition of Massai grass forage. The use of coated urea promoted greater of nitrogen use efficiency during all seasons of the year.(AU)

Sob a hipótese de aumento da eficiência da adubação nitrogenada quanto ao uso de diferentes fontes e doses de nitrogênio, aliado à maior produtividade e qualidade da forragem produzida, este trabalho foi conduzido com objetivo de avaliar a composição morfológica, as características estruturais e a composição química da forragem do Panicum maximum cv. Massai, cultivado sob diferentes fontes de ureia (comum e revestida por Policote®) e doses de nitrogênio (200, 400 e 600 kg ha-1 ano-1), durante as estações de outono, inverno e primavera de 2014, verão e outono de 2015. O experimento foi conduzido em Seropédica, RJ, sob delineamento de blocos completos casualizados em arranjo fatorial (3x2) + 1, com quatro repetições. O incremento de doses de nitrogênio promoveu maiores porcentagens de massa seca de lâminas foliares e menores porcentagens de material morto na massa de forragem, além de maiores densidades populacionais de per filhos e taxa de acúmulo de forragem do capim Massai, durante as estações. O uso da ureia revestida promoveu maiores teores de proteína bruta na forragem que a ureia comum em todas as estações. A intensificação da adubação nitrogenada reduz a eficiência do uso de nitrogênio, mas beneficia as características estruturais, o acúmulo de forragem, e a composição química do capim Massai. O uso de ureia revestida promove maior eficiência da utilização do nitrogênio durante todas as estações do ano.(AU)

Structural and physical-chemical analyses of sulfated polysaccharidesfrom the sea lettuce Ulva lactuca and their effects on thrombin generation / Análises estrutural e físico-química de polissacarídeos sulfatados da alface do mar Ulva lactuca e seus efeitos sobre geração de trombina

Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physicalchemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 μg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses.

Clorofícea Ulva lactuca possui polissacarídeos sulfatados (Ul-PSs) importantes biotecnologicamente, porém são desconhecidos seus potenciais sobre geração de trombina (GT). Analisaram-se as características estruturais e físico-químicas dos Ul-PSs como moduladores de GT. Digestão proteolítica rendeu (13,13%) extrato contendo sulfato (20,43%) e açúcares totais (65,72%), além de ulvana, como caracterizada por experimentos de ressonância magnética nuclear uni-/bi-dimensionais, consistindo de ramnose, xilose, glucose, ácido glucurônico e ligações glicosídicas tipos-α/-β. Fracionamento dos Ul-PSs por cromatografia de DEAE-celulose rendeu Ul-PS1 e Ul-PS2 (0,50 e 0,75 M de NaCl, respectivamente) mostrando sulfatação (15,72-18,04%) e açúcares totais (59,73- 60,58%) consistentes com o grau de densidade de carga por combinação de eletroforese em gel de agarose/poliacrilamida usando coramento sequencial com azul de toluidina e "stains-all", embora com diferenças quanto aos seus tamanhos (40 e >100 kDa, respectivamente). Por ambos os testes do tempo de tromboplastina parcial ativada (TTPA) e do tempo de protrombina, anticoagulação das frações foi detectada virtualmente pelo TTPA (0,39 e 0,43 UI, respectivamente) frente heparina (193 UI). Frações atuaram diferentemente sobre ambas as vias intrínsica/extrínsica na GT usando plasma humano diluído 60 vezes, com eficácias de 50% até 8,3 μg, enquanto em concentrações maiores sugeriram hipercoagulabilidade intrínsica visto que heparina aboliu ambos os sistemas em quantidades baixas. Ul-PSs bloqueiam GT, porém prevendo trombose em doses crescentes.

Correlation between rheological properties and capillary fixation for modelling creams

The Brazilian male beauty market occupies the second place in world consumption of cosmetics. Among the numerous products consumed by such audience is the capillary fixation mask, which is mainly composed by fixatives. These additives act on stabilizing the cosmetic emulsion, protecting the hair against moisture and also increasing the intensity of hair fixation. In this work, three formulations for modelling creams were prepared by different concentrations of the fixatives StylezeTM W20 and PVP K90 and their properties characterized by physicochemical, rheological and sensory analysis. The capillary masks produced were stable oil-in-water (O/W) emulsions with uniform droplets of 2.05-2.82 µm sizes and pseudoplastic thixotropic behavior (0.19 < n < 0.26). It was possible to correlate the increased concentration of PVP K90 to a greater thixotropy and an improved yarn fixation, despite the worsening in the spreadability of the formulations. These results suggest properly conducted rheological measurements can contribute to the prediction of the emulsion's sensory properties, which can save time and funds on the development of new cosmetics

Structural and physical-chemical analyses of sulfated polysaccharidesfrom the sea lettuce Ulva lactuca and their effects on thrombin generation / Análises estrutural e físico-química de polissacarídeos sulfatados da alface do mar Ulva lactuca e seus efeitos sobre geração de trombina

Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physicalchemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 μg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses.(AU)

Clorofícea Ulva lactuca possui polissacarídeos sulfatados (Ul-PSs) importantes biotecnologicamente, porém são desconhecidos seus potenciais sobre geração de trombina (GT). Analisaram-se as características estruturais e físico-químicas dos Ul-PSs como moduladores de GT. Digestão proteolítica rendeu (13,13%) extrato contendo sulfato (20,43%) e açúcares totais (65,72%), além de ulvana, como caracterizada por experimentos de ressonância magnética nuclear uni-/bi-dimensionais, consistindo de ramnose, xilose, glucose, ácido glucurônico e ligações glicosídicas tipos-α/-β. Fracionamento dos Ul-PSs por cromatografia de DEAE-celulose rendeu Ul-PS1 e Ul-PS2 (0,50 e 0,75 M de NaCl, respectivamente) mostrando sulfatação (15,72-18,04%) e açúcares totais (59,73- 60,58%) consistentes com o grau de densidade de carga por combinação de eletroforese em gel de agarose/poliacrilamida usando coramento sequencial com azul de toluidina e "stains-all", embora com diferenças quanto aos seus tamanhos (40 e >100 kDa, respectivamente). Por ambos os testes do tempo de tromboplastina parcial ativada (TTPA) e do tempo de protrombina, anticoagulação das frações foi detectada virtualmente pelo TTPA (0,39 e 0,43 UI, respectivamente) frente heparina (193 UI). Frações atuaram diferentemente sobre ambas as vias intrínsica/extrínsica na GT usando plasma humano diluído 60 vezes, com eficácias de 50% até 8,3 μg, enquanto em concentrações maiores sugeriram hipercoagulabilidade intrínsica visto que heparina aboliu ambos os sistemas em quantidades baixas. Ul-PSs bloqueiam GT, porém prevendo trombose em doses crescentes.(AU)

Agricultural answers and chemical composition of Massai grass under different nitrogen doses and urea sources / Respostas agronômicas e de composição química do capim Massai sob diferentes doses de nitrogênio e fontes de ureia

Under the hypothesis that modifying nitrogen sources and doses could increase nitrogen fertilization efficiency and productivity and improve forage quality, this study aimed to evaluate the morphological composition, structural characteristics and chemical composition of Panicum maximum cv. Massai forage cultivated under different urea sources (common and coated with Policote®) and nitrogen doses (200, 400 and 600 kg ha-1 year-1) during the autumn, winter and spring of 2014 and the summer and autumn of 2015. The experiment was carried out in Seropédica, RJ, under a randomized complete block design in a factorial arrangement (3x2) + 1 with four replications. High nitrogen rates promoted higher percentages of leaf blade dry mass and lower percentages of dead material dry mass in the forage mass and provided higher tiller population density and forage accumulation rate of Massai grass during the studied seasons. The use of coated urea promoted higher levels of crude protein in the forage than did the use of common urea in all seasons. The intensification of nitrogen fertilization reduced the nitrogen use efficiency but benefitted the structural characteristics, forage accumulation and chemical composition of Massai grass forage. The use of coated urea promoted greater of nitrogen use efficiency during all seasons of the year.

Sob a hipótese de aumento da eficiência da adubação nitrogenada quanto ao uso de diferentes fontes e doses de nitrogênio, aliado à maior produtividade e qualidade da forragem produzida, este trabalho foi conduzido com objetivo de avaliar a composição morfológica, as características estruturais e a composição química da forragem do Panicum maximum cv. Massai, cultivado sob diferentes fontes de ureia (comum e revestida por Policote®) e doses de nitrogênio (200, 400 e 600 kg ha-1 ano-1), durante as estações de outono, inverno e primavera de 2014, verão e outono de 2015. O experimento foi conduzido em Seropédica, RJ, sob delineamento de blocos completos casualizados em arranjo fatorial (3x2) + 1, com quatro repetições. O incremento de doses de nitrogênio promoveu maiores porcentagens de massa seca de lâminas foliares e menores porcentagens de material morto na massa de forragem, além de maiores densidades populacionais de per filhos e taxa de acúmulo de forragem do capim Massai, durante as estações. O uso da ureia revestida promoveu maiores teores de proteína bruta na forragem que a ureia comum em todas as estações. A intensificação da adubação nitrogenada reduz a eficiência do uso de nitrogênio, mas beneficia as características estruturais, o acúmulo de forragem, e a composição química do capim Massai. O uso de ureia revestida promove maior eficiência da utilização do nitrogênio durante todas as estações do ano.

Antioxidant activity and physicochemical analysis of passion fruit (Passiflora glandulosa Cav. ) pulp native to Cariri region / Atividade antioxidante e análise físico-química da polpa do maracujá (Passiflora glandulosa Cav. ) nativo da região do Cariri

The objective of this study was to determine the proximal composition, the physical and chemical characteristics and the in vitro antioxidant capacity (DPPH, ABTS and FRAP) of the pulp of the wild passion fruit (Passiflora glandulosa Cav.) from Cariri region, Ceara, Brazil. The results showed that the proximal composition and the caloric value of this passion fruit are similar to other species, but with a high ascorbic acid content. The fruit pulp showed low level of antioxidant activity and low level of polyphenolic compounds followed by three methodologies used. Due to high levels of titratable acidity (3.52) and total soluble solids (17.80 Brix), this fruit can be considered as a high value fruit for commerce. However, it is a species of passion fruit with few studies described in the literature, and more research is needed to assess its nutritional and functional potential.(AU)

O objetivo do estudo foi determinar a composição centesimal, as características físicas equímicas e a capacidade antioxidante (DPPH, ABTS e FRAP) in vitro da polpa do maracujá-do-mato(Passiflora glandulosa Cav.) da região do Cariri, Ceará, Brasil. Os resultados demonstraram que a composiçãocentesimal e o valor calórico desse tipo de maracujá são similares a outras espécies, mas com nível elevadode ácido ascórbico. A polpa apresentou baixo teor de polifenóis e baixa atividade antioxidante para as trêsmetodologias aplicadas. Em razão dos altos níveis de acidez titulável (3,52) e sólidos solúveis totais (17,80°Brix), este fruto pode ser considerado como um fruto de alto valor para o comércio. Porém, esta é umaespécie de maracujá pouco descrita na literatura, sendo necessárias mais análises para avaliar seu potencialvalor nutricional e funcional.(AU)

Physicochemical and microbial analysis of feces from horses fed diets containing citrus pulp / Análises fisicoquímicas e microbiológicas de fezes de equinos alimentados com dietas que contém polpa cítrica

This study aimed to evaluate the effect of diets containing increasing levels of citrus pulp on the physicochemical and microbiological characteristics of horses feces. Five mares, at an average age of 3.5 years old and body weight of 492 ± 44.5 kg were arranged in a 5 x 5 Latin Square. The experimental diet consisted of 60% coast-cross hay and 40 % of concentrate with increasing levels of citrus pulp (0, 7, 14, 21, and 28 %). To determine the fecal pH, samples were collected directly from the floor, immediately after defecation, in the first feces of the day at 07:00 a.m., and color and fecal consistency were evaluated. For microbiological analysis, an aliquot was reserved in plastic bags, frozen, and sent to the microbiological laboratory for further analysis. Lactic acid bacteria were counted for Lactobacillus spp. and Streptococcus spp. from fecal samples under anaerobic conditions. The diet produced differences (P 0.05) in feces consistency: 98% of the animals had normal and firm stools, while 2% were loose ruminant-type feces. We observed no difference (P 0.05) for color, verifying 100% of the animals of greenish feces, normal for equines. There was no effect (P>0.05) on pH and on the number of Lactobacillus spp. and Streptococcus spp. The inclusion of up to 28% citruspulp concentrates for horses did not promote change in the physicochemical characteristics and on the population of lactic acid-producing bacteria in feces.

O presente estudo teve como objetivo avaliar o efeito de dietas com níveis crescentes de polpa cítrica nas análises físico-químicas e microbiológicas nas fezes de cavalos. Cinco éguas, com idade média de 3,5 anos e peso corporal de 492 ± 44.5 kg foram dispostas em um quadrado latino 5x5. As dietas experimentais consistiram de 60% de feno coastcross e 40% de concentrado com níveis crescentes de polpa cítrica (0, 7, 14, 21, 28%). Para determinar o pH fecal foram colhidas amostras (diretamente do reto) das primeiras fezes do dia às 07:00 min e foram avaliados as características de cor e consistência fecal. Para as análises microbiológicas, um alíquota foi reservada em plásticos, congeladas e enviadas para o laboratório para posterior análise. As bactérias láticas foram enumeradas por Lactobacillus spp. e Streptococcus spp. a partir das amostras fecais, em condições anaeróbicas. Não houve efeito (P 0,05) para a consistência fecal, 98% dos animais apresentaram fezes normais e firmes, enquanto 2% apresentaram fezes do tipo soltas. Não houve diferença (P 0,05) para a cor sendo que 100%  dos animais apresentaram fezes verdes e normais. Não houve efeito (P>0,05) no pH e no número   de Lactobacillus spp. e Streptococcus spp. A inclusão de até 28% de polpa cítrica no concentrado para equinos não promove mudanças nas características físico-químicas e na população de bactérias produtoras de ácido lático nas fezes.

Isotopic of nitrogen and chemical analysis of deferred grazing marandu palisadegrass fertilized with urea and poultry litter / Análise isotópica de nitrogênio e química do capim-marandu adubado com ureia e cama de frango e diferido

This study aimed to evaluate and quantify the methodology of stable isotopes, 15N values for the organic fertilizer (chicken manure) and inorganic (urea). In addition to evaluating the incorporation rate, chemical half-life and analysis thereof, for possible traceability through the pasture fertilized with different sources of N. The technique of employment with use of tracer stable isotope 15N possible to estimate the changes in the N soil, even in the face of the various changes that take place concurrently. The methodology determines the path of both N isotopes (15N and 14N), providing information about the system and estimating N transformation rates The experimental work was conducted at the Faculty of Veterinary Medicine and Animal Science, UNESP Botucatu. An area of 576m2 was used for the parcels, which were divided between the 3 treatments with 4 repetitions each: T1 - control treatment without fertilization; T2 - fertilization with urea and T3 - organic fertilizer with poultry litter. The grass was cut every 15 days, three months after fertilization, for a total of four periods of grazing exclusion. Experimental samples were analyzed for the isotopic nitrogen and crude protein, neutral detergent fiber, acid detergent fiber, cellulose, hemicellulose and lignin composition. Degradation of poultry litter, the sand bag method was analyzed by linear regression...(AU)

Objetivou-se avaliar e quantificar, pela metodologia dos isótopos estáveis, os valores de 15N para o adubo orgânico (cama de frango) e inorgânico (ureia). Além de avaliar a taxa de incorporação, meia vida e analises química dos mesmos, para uma possível rastreabilidade por meio da pastagem adubada com diferentes fontes de N. O emprego da técnica com utilização do traçador do isótopo estável 15N permitem estimar as modificações do N no solo, mesmo diante das variadas transformações que acontecem concomitantemente. A metodologia determina o caminho de ambos os isótopos de N (15N e 14N), fornecendo informações acerca do sistema e estimando as taxas de transformação do N. O presente estudo teve por objetivo determinar o valor isotópico do 15N e composição química em condições naturais do capim-marandu (Urochloa brizantha cv. Marandu), sob o diferimento em função da adubação utilizada, em quatro cortes após a vedação. O trabalho foi desenvolvido na Faculdade de Medicina e Veterinária e Zootecnia da UNESP de Botucatu. A área, 576m2, utilizada para as parcelas foi dividida em 3 tratamentos com 4 repetições cada, segundo os tratamentos: T1 - tratamento testemunho sem adubação; T2 - adubação com ureia e T3 adubação orgânica com cama de frango. Após três meses, o capim foi cortado a cada 15 dias, totalizando quatro períodos de vedação. As amostras colhidas no experimento foram...(AU)

Isotopic of nitrogen and chemical analysis of deferred grazing marandu palisadegrass fertilized with urea and poultry litter / Análise isotópica de nitrogênio e química do capim-marandu adubado com ureia e cama de frango e diferido

This study aimed to evaluate and quantify the methodology of stable isotopes, 15N values for the organic fertilizer (chicken manure) and inorganic (urea). In addition to evaluating the incorporation rate, chemical half-life and analysis thereof, for possible traceability through the pasture fertilized with different sources of N. The technique of employment with use of tracer stable isotope 15N possible to estimate the changes in the N soil, even in the face of the various changes that take place concurrently. The methodology determines the path of both N isotopes (15N and 14N), providing information about the system and estimating N transformation rates The experimental work was conducted at the Faculty of Veterinary Medicine and Animal Science, UNESP Botucatu. An area of 576m2 was used for the parcels, which were divided between the 3 treatments with 4 repetitions each: T1 - control treatment without fertilization; T2 - fertilization with urea and T3 - organic fertilizer with poultry litter. The grass was cut every 15 days, three months after fertilization, for a total of four periods of grazing exclusion. Experimental samples were analyzed for the isotopic nitrogen and crude protein, neutral detergent fiber, acid detergent fiber, cellulose, hemicellulose and lignin composition. Degradation of poultry litter, the sand bag method was analyzed by linear regression...

Objetivou-se avaliar e quantificar, pela metodologia dos isótopos estáveis, os valores de 15N para o adubo orgânico (cama de frango) e inorgânico (ureia). Além de avaliar a taxa de incorporação, meia vida e analises química dos mesmos, para uma possível rastreabilidade por meio da pastagem adubada com diferentes fontes de N. O emprego da técnica com utilização do traçador do isótopo estável 15N permitem estimar as modificações do N no solo, mesmo diante das variadas transformações que acontecem concomitantemente. A metodologia determina o caminho de ambos os isótopos de N (15N e 14N), fornecendo informações acerca do sistema e estimando as taxas de transformação do N. O presente estudo teve por objetivo determinar o valor isotópico do 15N e composição química em condições naturais do capim-marandu (Urochloa brizantha cv. Marandu), sob o diferimento em função da adubação utilizada, em quatro cortes após a vedação. O trabalho foi desenvolvido na Faculdade de Medicina e Veterinária e Zootecnia da UNESP de Botucatu. A área, 576m2, utilizada para as parcelas foi dividida em 3 tratamentos com 4 repetições cada, segundo os tratamentos: T1 - tratamento testemunho sem adubação; T2 - adubação com ureia e T3 adubação orgânica com cama de frango. Após três meses, o capim foi cortado a cada 15 dias, totalizando quatro períodos de vedação. As amostras colhidas no experimento foram...

Physicochemical and microbial analysis of feces from horses fed diets containing citrus pulp / Análises fisicoquímicas e microbiológicas de fezes de equinos alimentados com dietas que contém polpa cítrica

This study aimed to evaluate the effect of diets containing increasing levels of citrus pulp on the physicochemical and microbiological characteristics of horses feces. Five mares, at an average age of 3.5 years old and body weight of 492 ± 44.5 kg were arranged in a 5 x 5 Latin Square. The experimental diet consisted of 60% coast-cross hay and 40 % of concentrate with increasing levels of citrus pulp (0, 7, 14, 21, and 28 %). To determine the fecal pH, samples were collected directly from the floor, immediately after defecation, in the first feces of the day at 07:00 a.m., and color and fecal consistency were evaluated. For microbiological analysis, an aliquot was reserved in plastic bags, frozen, and sent to the microbiological laboratory for further analysis. Lactic acid bacteria were counted for Lactobacillus spp. and Streptococcus spp. from fecal samples under anaerobic conditions. The diet produced differences (P 0.05) in feces consistency: 98% of the animals had normal and firm stools, while 2% were loose ruminant-type feces. We observed no difference (P 0.05) for color, verifying 100% of the animals of greenish feces, normal for equines. There was no effect (P>0.05) on pH and on the number of Lactobacillus spp. and Streptococcus spp. The inclusion of up to 28% citruspulp concentrates for horses did not promote change in the physicochemical characteristics and on the population of lactic acid-producing bacteria in feces.(AU)

O presente estudo teve como objetivo avaliar o efeito de dietas com níveis crescentes de polpa cítrica nas análises físico-químicas e microbiológicas nas fezes de cavalos. Cinco éguas, com idade média de 3,5 anos e peso corporal de 492 ± 44.5 kg foram dispostas em um quadrado latino 5x5. As dietas experimentais consistiram de 60% de feno coastcross e 40% de concentrado com níveis crescentes de polpa cítrica (0, 7, 14, 21, 28%). Para determinar o pH fecal foram colhidas amostras (diretamente do reto) das primeiras fezes do dia às 07:00 min e foram avaliados as características de cor e consistência fecal. Para as análises microbiológicas, um alíquota foi reservada em plásticos, congeladas e enviadas para o laboratório para posterior análise. As bactérias láticas foram enumeradas por Lactobacillus spp. e Streptococcus spp. a partir das amostras fecais, em condições anaeróbicas. Não houve efeito (P 0,05) para a consistência fecal, 98% dos animais apresentaram fezes normais e firmes, enquanto 2% apresentaram fezes do tipo soltas. Não houve diferença (P 0,05) para a cor sendo que 100%  dos animais apresentaram fezes verdes e normais. Não houve efeito (P>0,05) no pH e no número   de Lactobacillus spp. e Streptococcus spp. A inclusão de até 28% de polpa cítrica no concentrado para equinos não promove mudanças nas características físico-químicas e na população de bactérias produtoras de ácido lático nas fezes.(AU)

Chemical composition and pH of the meat of broilers submitted to pre-slaughter heat stress

In order to evaluate the chemical composition and pH of chicken meat subjected to heat stress during the pre-slaughter rest time, an experiment was conducted in a slaughterhouse. Twenty-five birds were randomly selected and housed in rooms without air conditioning for a 3-hour period, with an average temperature of 33C and relative humidity of 83%. After slaughter, the carcasses were deboned and prime cuts were chilled (between 0 and 4 C) for 24 hours for analysis of moisture levels, protein, fat, ash, and pH. The humidity values were 72.80, 71.47, and 70.30%, protein values were 16.81, 14.90, and 15.10%, lipid values were 0.78, 3.30, and 5.80%, ash values were 0.81, 0.88, and 0.89%, and pH values were 5.30, 6.10, and 6.54 for breast, thigh, and drumstick, respectively. Heat stress for 3 hours before slaughter led to changes in the chemical composition and pH of the chicken, which establishes an anomaly in the flesh of the PSE type (pale, soft, and exudative). It is evident that pre-slaughter management is important to ensure animal welfare and consequently high meat quality.(AU)

Chemical composition and pH of the meat of broilers submitted to pre-slaughter heat stress

In order to evaluate the chemical composition and pH of chicken meat subjected to heat stress during the pre-slaughter rest time, an experiment was conducted in a slaughterhouse. Twenty-five birds were randomly selected and housed in rooms without air conditioning for a 3-hour period, with an average temperature of 33C and relative humidity of 83%. After slaughter, the carcasses were deboned and prime cuts were chilled (between 0 and 4 C) for 24 hours for analysis of moisture levels, protein, fat, ash, and pH. The humidity values were 72.80, 71.47, and 70.30%, protein values were 16.81, 14.90, and 15.10%, lipid values were 0.78, 3.30, and 5.80%, ash values were 0.81, 0.88, and 0.89%, and pH values were 5.30, 6.10, and 6.54 for breast, thigh, and drumstick, respectively. Heat stress for 3 hours before slaughter led to changes in the chemical composition and pH of the chicken, which establishes an anomaly in the flesh of the PSE type (pale, soft, and exudative). It is evident that pre-slaughter management is important to ensure animal welfare and consequently high meat quality.