Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 37
Filter
Add more filters










Publication year range
1.
Toxins (Basel) ; 6(6): 1813-36, 2014 Jun 10.
Article in English | MEDLINE | ID: mdl-24918360

ABSTRACT

Harmful algal blooms expose humans and animals to microcystins (MCs) through contaminated drinking water. While hepatotoxicity following acute exposure to MCs is well documented, neurotoxicity after sub-lethal exposure is poorly understood. We developed a novel statistical approach using a generalized linear model and the quasibinomial family to analyze neurotoxic effects in adult Caenorhabditis elegans exposed to MC-LR or MC-LF for 24 h. Selective effects of toxin exposure on AWA versus AWC sensory neuron function were determined using a chemotaxis assay. With a non-monotonic response MCs altered AWA but not AWC function, and MC-LF was more potent than MC-LR. To probe a potential role for protein phosphatases (PPs) in MC neurotoxicity, we evaluated the chemotactic response in worms exposed to the PP1 inhibitor tautomycin or the PP2A inhibitor okadaic acid for 24 h. Okadaic acid impaired both AWA and AWC function, while tautomycin had no effect on function of either neuronal cell type at the concentrations tested. These findings suggest that MCs alter the AWA neuron at concentrations that do not cause AWC toxicity via mechanisms other than PP inhibition.


Subject(s)
Bacterial Toxins/pharmacology , Caenorhabditis elegans/drug effects , Chemoreceptor Cells/drug effects , Chemotaxis/drug effects , Microcystins/pharmacology , Nerve Tissue Proteins/antagonists & inhibitors , Neurotoxins/pharmacology , Animals , Behavior, Animal/drug effects , Caenorhabditis elegans/enzymology , Caenorhabditis elegans/metabolism , Caenorhabditis elegans Proteins/antagonists & inhibitors , Caenorhabditis elegans Proteins/metabolism , Chemoreceptor Cells/enzymology , Chemoreceptor Cells/metabolism , Enzyme Inhibitors/pharmacology , Isoenzymes/antagonists & inhibitors , Isoenzymes/metabolism , Marine Toxins , Nerve Tissue Proteins/metabolism , Olfactory Receptor Neurons/drug effects , Olfactory Receptor Neurons/enzymology , Olfactory Receptor Neurons/metabolism , Osmolar Concentration , Protein Phosphatase 1/antagonists & inhibitors , Protein Phosphatase 1/metabolism , Protein Phosphatase 2/antagonists & inhibitors , Protein Phosphatase 2/metabolism , Reproducibility of Results
2.
Respir Physiol Neurobiol ; 188(2): 83-93, 2013 Aug 15.
Article in English | MEDLINE | ID: mdl-23727159

ABSTRACT

Stimulation of the carotid body (CB) chemoreceptors by hypercapnia triggers a reflex ventilatory response via a cascade of cellular events, which includes generation of cAMP. However, it is not known if molecular CO2/HCO3(-) and/or H(+) mediate this effect and how these molecules contribute to cAMP production. We previously reported that the CB highly expresses HCO3(-)-sensitive soluble adenylyl cyclase (sAC). In the present study we systematically characterize the role of sAC in the CB, comparing the effect of isohydric hypercapnia (IH) in cAMP generation through activation of sAC or transmembrane-adenylyl cyclase (tmAC). Pharmacological deactivation of sAC and tmAC decreased the CB cAMP content in normocapnia and IH with no differences between these two conditions. Changes from normocapnia to IH did not effect the degree of PKA activation and the carotid sinus nerve discharge frequency. sAC and tmAC are functional in CB but intracellular elevations in CO2/HCO3(-) in IH conditions on their own are insufficient to further activate these enzymes, suggesting that the hypercapnic response is dependent on secondary acidosis.


Subject(s)
Adenylyl Cyclases/metabolism , Bicarbonates/pharmacology , Chemoreceptor Cells/drug effects , Action Potentials/drug effects , Adenylyl Cyclases/classification , Adenylyl Cyclases/genetics , Animals , Animals, Newborn , Carotid Body/cytology , Carotid Body/metabolism , Chemoreceptor Cells/enzymology , Colforsin/pharmacology , Cyclic AMP/metabolism , Cyclic AMP-Dependent Protein Kinases , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Ganglia, Sensory/cytology , Gene Expression Regulation, Enzymologic/drug effects , Hydrogen-Ion Concentration , Hypercapnia/enzymology , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Nucleotides, Cyclic/metabolism , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley
3.
PLoS One ; 7(12): e50689, 2012.
Article in English | MEDLINE | ID: mdl-23226535

ABSTRACT

Bacillus subtilis use three systems for adaptation during chemotaxis. One of these systems involves two interacting proteins, CheC and CheD. CheD binds to the receptors and increases their ability to activate the CheA kinase. CheD also binds CheC, and the strength of this interaction is increased by phosphorylated CheY. CheC is believed to control the binding of CheD to the receptors in response to the levels of phosphorylated CheY. In addition to their role in adaptation, CheC and CheD also have separate enzymatic functions. CheC is a CheY phosphatase and CheD is a receptor deamidase. Previously, we demonstrated that CheC's phosphatase activity plays a minor role in chemotaxis whereas its ability to bind CheD plays a major one. In the present study, we demonstrate that CheD's deamidase activity also plays a minor role in chemotaxis whereas its ability to bind CheC plays a major one. In addition, we quantified the interaction between CheC and CheD using surface plasmon resonance. These results suggest that the most important features of CheC and CheD are not their enzymatic activities but rather their roles in adaptation.


Subject(s)
Bacillus subtilis/cytology , Bacillus subtilis/enzymology , Bacterial Proteins/metabolism , Chemoreceptor Cells/cytology , Chemoreceptor Cells/enzymology , Chemotaxis , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Blotting, Western , Carboxypeptidases/metabolism , Crystallography, X-Ray , Enzyme Assays , Kinetics , Models, Molecular , Molecular Sequence Data , Mutation/genetics , Protein Binding , Reproducibility of Results , Sequence Alignment , Surface Plasmon Resonance , Thermotoga maritima/enzymology
4.
J Biol Chem ; 287(16): 12634-44, 2012 Apr 13.
Article in English | MEDLINE | ID: mdl-22375004

ABSTRACT

G protein-coupled receptor kinases (GRKs) are key regulators of signal transduction that specifically phosphorylate activated G protein-coupled receptors (GPCRs) to terminate signaling. Biochemical and crystallographic studies have provided great insight into mammalian GRK2/3 interactions and structure. However, despite extensive in vitro characterization, little is known about the in vivo contribution of these described GRK structural domains and interactions to proper GRK function in signal regulation. We took advantage of the disrupted chemosensory behavior characteristic of Caenorhabditis elegans grk-2 mutants to discern the interactions required for proper in vivo Ce-GRK-2 function. Informed by mammalian crystallographic and biochemical data, we introduced amino acid substitutions into the Ce-grk-2 coding sequence that are predicted to selectively disrupt GPCR phosphorylation, Gα(q/11) binding, Gßγ binding, or phospholipid binding. Changing the most amino-terminal residues, which have been shown in mammalian systems to be required specifically for GPCR phosphorylation but not phosphorylation of alternative substrates or recruitment to activated GPCRs, eliminated the ability of Ce-GRK-2 to restore chemosensory signaling. Disrupting interaction between the predicted Ce-GRK-2 amino-terminal α-helix and kinase domain, posited to stabilize GRKs in their active ATP- and GPCR-bound conformation, also eliminated Ce-GRK-2 chemosensory function. Finally, although changing residues within the RH domain, predicted to disrupt interaction with Gα(q/11), did not affect Ce-GRK-2 chemosensory function, disruption of the predicted PH domain-mediated interactions with Gßγ and phospholipids revealed that both contribute to Ce-GRK-2 function in vivo. Combined, we have demonstrated functional roles for broadly conserved GRK2/3 structural domains in the in vivo regulation of organismal behavior.


Subject(s)
Caenorhabditis elegans Proteins/chemistry , Caenorhabditis elegans Proteins/metabolism , Caenorhabditis elegans/enzymology , G-Protein-Coupled Receptor Kinase 2/chemistry , G-Protein-Coupled Receptor Kinase 2/metabolism , G-Protein-Coupled Receptor Kinases/chemistry , G-Protein-Coupled Receptor Kinases/metabolism , Amino Acid Sequence , Animals , Animals, Genetically Modified , Behavior, Animal/physiology , Caenorhabditis elegans/genetics , Caenorhabditis elegans Proteins/genetics , Chemoreceptor Cells/enzymology , G-Protein-Coupled Receptor Kinase 2/genetics , G-Protein-Coupled Receptor Kinases/genetics , Molecular Sequence Data , Mutagenesis , Neurons/enzymology , Phosphorylation/physiology , Protein Structure, Tertiary , Signal Transduction/physiology
5.
Adv Exp Med Biol ; 648: 235-41, 2009.
Article in English | MEDLINE | ID: mdl-19536486

ABSTRACT

UNLABELLED: Peripheral arterial chemoreceptors in the carotid body (CB) are modulated by pH/CO(2). Soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate ions (HCO(3)). Because CO(2)/HCO(3) mediates depolarization in chemoreceptors, we hypothesized that sAC mRNA would be expressed in the CB, and its expression and function would be regulated by CO(2)/HCO(3).Sprague-Dawley rats at postnatal days 16-17 were used to compare sAC mRNA gene expression between CB and non-chemosensitive tissues: superior cervical (SCG), petrosal (PG) and nodose ganglia (NG) by quantitative real time-PCR. Rat sAC gene expression was standardized to the expression of GAPDH (housekeeping gene) and the data were analyzed with the Pfaffl method. Gene and protein expression, and sAC regulation in the testis was used as a positive control. To determine the regulation of sAC mRNA expression and activity, all tissues were exposed to increasing concentrations of bicarbonate (0, 24, 44 mM, titrated with CO(2) and maintained a constant pH of 7.40). RESULTS: sAC mRNA expression was between 2-11% of CB expression in the SCG, PG and NG. Furthermore, only in the CB did HCO(3) upregulate sAC gene expression and increase cAMP levels. CONCLUSION: sAC mRNA and protein expression is present in peripheral arterial chemoreceptors and non-chemoreceptors. In the CB, CO(2)/HCO(3) not only activated sAC but also regulated its expression, suggesting that sAC may be involved in the regulation of cAMP levels in response to hyper/hypocapnia.


Subject(s)
Adenylyl Cyclases/genetics , Adenylyl Cyclases/metabolism , Bicarbonates/pharmacology , Carotid Body/drug effects , Carotid Body/enzymology , Chemoreceptor Cells/drug effects , Gene Expression Regulation, Enzymologic/drug effects , Adenylyl Cyclases/chemistry , Animals , Carbon Dioxide/metabolism , Carbon Dioxide/pharmacology , Carotid Body/cytology , Carotid Body/metabolism , Chemoreceptor Cells/enzymology , Chemoreceptor Cells/metabolism , Cyclic AMP/metabolism , In Vitro Techniques , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Solubility
6.
Gastroenterology ; 137(1): 231-41, 241.e1-10, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19208342

ABSTRACT

BACKGROUND & AIMS: Gastrin is a key regulator of gastric acid secretion. We aimed to isolate pure G cells to identify the mechanistic basis of luminal- and strain-mediated regulation. METHODS: Using gradient centrifugation and fluorescence-activated cell sorting, rat G cells were prepared and luminal, neural, hormonal, and mechanical activation of secretion and signaling pathways studied. RESULTS: Pure G-cell preparations (>97%) were isolated. Reverse-transcription polymerase chain reaction identified neural, hormonal, bacterial, and luminal G protein-coupled receptors, and immunostaining visualized specific sweet/bitter receptors and the tastant-associated G protein alpha-gustducin. Gastrin release was stimulated by forskolin (adenosine 3',5'-cyclic monophosphate [cAMP] inducer, 10 micromol/L; >3-fold), potentiated by 3-isobutyl-1-methylxanthine (IBMX; phosphodiesterase type 5 inhibitor and adenosine antagonist, 10 micromol/L) and phorbol myristate acetate (phorbol ester, 10 micromol/L), and inhibited by H-89 (protein kinase A inhibitor, 10 micromol/L), PD98059 (MEK1 inhibitor, 0.1 micromol/L), and wortmannin (phosphatidylinositol 3-kinase inhibitor, 1 nmol/L). Gastrin release was stimulated by neuronal G protein-coupled receptor ligands, pituitary adenylate cyclase-activating protein (20 pmol/L, >8-fold) and bombesin (0.1 micromol/L, 8-fold) through cAMP signaling. The tastants sucralose, glucose, caffeine, denatonium, and the vanilloid receptor activator capsaicin all stimulated secretion (>3-fold), as did bacterial lipopolysaccharides Salmonella enteritidis (0.24 nmol/L, 5-fold) greater than Helicobacter pylori (0.57 micromol/L, 3-fold). Secretion was associated with elevated cAMP levels (approximately 2-fold) and could be inhibited by H-89 and PD98059 and potentiated by IBMX and cholera toxin (250 microg/mL). Bacterially mediated secretion also involved activation of nuclear factor kappaB and the c-Jun-N-terminal kinase pathway. Mechanical strain stimulated (2-fold to 8-fold) gastrin release, and decreasing pH from 7.4 to 5.5 inhibited release. The adenosine receptor 2B antagonist MRS1754 inhibited mechanically induced gastrin release. CONCLUSIONS: G cells are luminal sampling chemomechanosensory cells whose secretion is regulated by neural, hormonal, luminal, and mechanical factors through protein kinase A activation, cAMP signaling, and mitogen-activated protein kinase phosphorylation.


Subject(s)
Chemoreceptor Cells/metabolism , Gastrin-Secreting Cells/metabolism , Gastrins/metabolism , Mechanotransduction, Cellular , Animals , Bacterial Toxins/pharmacology , Cell Separation , Cell Survival , Cells, Cultured , Chemoreceptor Cells/drug effects , Chemoreceptor Cells/enzymology , Chemoreceptor Cells/ultrastructure , Cyclic AMP/metabolism , Cyclic AMP-Dependent Protein Kinases/metabolism , Dose-Response Relationship, Drug , Enzyme Activation , Enzyme Activators/pharmacology , Enzyme Inhibitors/pharmacology , Flow Cytometry , Gastrin-Secreting Cells/drug effects , Gastrin-Secreting Cells/enzymology , Gastrin-Secreting Cells/ultrastructure , Hydrogen-Ion Concentration , Lipopolysaccharides/pharmacology , Male , Mechanotransduction, Cellular/drug effects , Mitogen-Activated Protein Kinases/metabolism , Neurotransmitter Agents/metabolism , Phosphorylation , Rats , Rats, Sprague-Dawley , Receptors, G-Protein-Coupled/metabolism , Stress, Mechanical
8.
Am J Physiol Regul Integr Comp Physiol ; 296(1): R102-18, 2009 Jan.
Article in English | MEDLINE | ID: mdl-18971354

ABSTRACT

Heme oxygenase has been linked to the oxygen-sensing function of the carotid body, pulmonary vasculature, cerebral vasculature, and airway smooth muscle. We have shown previously that the cardiorespiratory regions of the rostral ventrolateral medulla are excited by local hypoxia and that heme oxygenase-2 (HO-2) is expressed in the hypoxia-chemosensitive regions of the rostral ventrolateral medulla (RVLM), the respiratory pre-Bötzinger complex, and C1 sympathoexcitatory region. To determine whether heme oxygenase is necessary for the hypoxic-excitation of dissociated RVLM neurons (P1) cultured on confluent medullary astrocytes (P5), we examined their electrophysiological responses to hypoxia (NaCN and low Po(2)) using the whole-cell perforated patch clamp technique before and after blocking heme oxygenase with tin protoporphyrin-IX (SnPP-IX). Following the electrophysiological recording, immunocytochemistry was performed on the recorded neuron to correlate the electrophysiological response to hypoxia with the expression of HO-2. We found that the responses to NaCN and hypoxia were similar. RVLM neurons responded to NaCN and low Po(2) with either depolarization or hyperpolarization and SnPP-IX blocked the depolarization response of hypoxia-excited neurons to both NaCN and low Po(2) but had no effect on the hyperpolarization response of hypoxia-depressed neurons. Consistent with this observation, HO-2 expression was present only in the hypoxia-excited neurons. We conclude that RVLM neurons are excited by hypoxia via a heme oxygenase-dependent mechanism.


Subject(s)
Chemoreceptor Cells/enzymology , Heme Oxygenase (Decyclizing)/metabolism , Medulla Oblongata/enzymology , Neurons/enzymology , Oxygen/metabolism , Signal Transduction , Action Potentials , Animals , Animals, Newborn , Astrocytes/metabolism , Cell Hypoxia , Cells, Cultured , Chemoreceptor Cells/drug effects , Coculture Techniques , Enzyme Inhibitors/pharmacology , Heme Oxygenase (Decyclizing)/antagonists & inhibitors , Immunohistochemistry , Medulla Oblongata/cytology , Medulla Oblongata/drug effects , Metalloporphyrins/pharmacology , Neurons/drug effects , Patch-Clamp Techniques , Protoporphyrins/pharmacology , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects , Sodium Cyanide/pharmacology , Time Factors
9.
BMC Neurosci ; 8: 65, 2007 Aug 06.
Article in English | MEDLINE | ID: mdl-17683617

ABSTRACT

BACKGROUND: Drosophila flies explore the environment very efficiently in order to colonize it. They explore collectively, not individually, so that when a few land on a food spot, they attract the others by signs. This behaviour leads to aggregation of individuals and optimizes the screening of mates and egg-laying on the most favourable food spots. RESULTS: Flies perform cycles of exploration/aggregation depending on the resources of the environment. This behavioural ecology constitutes an excellent model for analyzing simultaneous processing of neurosensory information. We reasoned that the decision of flies to land somewhere in order to achieve aggregation is based on simultaneous integration of signals (visual, olfactory, acoustic) during their flight. On the basis of what flies do in nature, we designed laboratory tests to analyze the phenomenon of neuronal coincidence. We screened many mutants of genes involved in neuronal metabolism and the synaptic machinery. CONCLUSION: Mutants of NO-dependent cyclase show a specifically-marked behaviour phenotype, but on the other hand they are associated with moderate biochemical defects. We show that these mutants present errors in integrative and/or coincident processing of signals, which are not reducible to the functions of the peripheral sensory cells.


Subject(s)
Drosophila melanogaster/enzymology , Exploratory Behavior/physiology , Guanylate Cyclase/metabolism , Nervous System/enzymology , Neurons/enzymology , Receptors, Cytoplasmic and Nuclear/metabolism , Signal Transduction/genetics , Animals , Animals, Genetically Modified , Brain/enzymology , Brain/physiopathology , Chemoreceptor Cells/enzymology , Drosophila melanogaster/genetics , Feeding Behavior/physiology , Gene Expression Regulation, Enzymologic/genetics , Guanylate Cyclase/genetics , Mechanoreceptors/enzymology , Mutation/genetics , Nervous System/physiopathology , Neurons, Afferent/enzymology , Peripheral Nervous System/enzymology , Peripheral Nervous System/physiopathology , Phenotype , Receptors, Cytoplasmic and Nuclear/genetics , Smell/genetics , Soluble Guanylyl Cyclase , Taste/genetics , Wings, Animal/innervation
10.
Respir Physiol Neurobiol ; 157(1): 45-54, 2007 Jul 01.
Article in English | MEDLINE | ID: mdl-17223613

ABSTRACT

O(2)-sensing in the carotid body occurs in neuroectoderm-derived type I glomus cells where hypoxia elicits a complex chemotransduction cascade involving membrane depolarization, Ca(2+) entry and the release of excitatory neurotransmitters. Efforts to understand the exquisite O(2)-sensitivity of these cells currently focus on the coupling between local P(O2) and the open-closed state of K(+)-channels. Amongst multiple competing hypotheses is the notion that K(+)-channel activity is mediated by a phagocytic-like multisubunit enzyme, NADPH oxidase, which produces reactive oxygen species (ROS) in proportion to the prevailing P(O2). In O(2)-sensitive cells of lung neuroepithelial bodies (NEB), multiple studies confirm that ROS levels decrease in hypoxia, and that E(M) and K(+)-channel activity are indeed controlled by ROS produced by NADPH oxidase. However, recent studies in our laboratories suggest that ROS generated by a non-phagocyte isoform of the oxidase are important contributors to chemotransduction, but that their role in type I cells differs fundamentally from the mechanism utilized by NEB chemoreceptors. Data indicate that in response to hypoxia, NADPH oxidase activity is increased in type I cells, and further, that increased ROS levels generated in response to low-O(2) facilitate cell repolarization via specific subsets of K(+)-channels.


Subject(s)
Carotid Body/enzymology , Chemoreceptor Cells/enzymology , Mechanotransduction, Cellular/physiology , NADPH Oxidases/metabolism , Animals , Arteries/enzymology , Arteries/innervation , Humans , Potassium Channels/metabolism , Reactive Oxygen Species/metabolism
11.
J Comp Neurol ; 494(3): 485-94, 2006 Jan 20.
Article in English | MEDLINE | ID: mdl-16320252

ABSTRACT

The anterior piriform cortex (APC) has been shown to be an essential brain structure for the detection of dietary indispensable amino acid (IAA) deficiency, but little has been known about possible molecular detection mechanisms. Increased phosphorylation of the alpha-subunit of the eukaryotic initiation factor 2alpha (eIF2alpha) has been directly linked to amino acid deficiency in yeast. Recently, we have shown increased phosphorylation of eIF2alpha (p-eIF2alpha) in the rat APC 20 minutes after ingestion of an IAA-deficient meal. We suggest that if phosphorylation of eIF2alpha is an important mechanism in detection of IAA deficiency, then APC neurons that show p-eIF2alpha should also show molecular evidence of potentiation. The present research demonstrates increased expression and co-localization of p-eIF2alpha and phosphorylated extracellular signal-regulated protein kinase 1/2 (p-ERK1/2) in APC neurons, but not in the primary motor or agranular insular cortices in response to an IAA-deficient diet. ERK1/2 is an element of the mitogen-activated protein kinase cascade, an intraneuronal signaling mechanism associated with neuronal activation. The region of the APC that responds to IAA deficiency with increased p-eIF2alpha and p-ERK1/2 labeling ranges from 3.1 to 2.5 mm rostral of bregma. Within this region, only a few neurons respond to IAA deficiency with co-localization of abundant p-eIF2alpha and p-ERK1/2. These chemosensory neurons probably detect IAA deficiency and generate neuronal signaling to other portions of the brain, changing feeding behavior.


Subject(s)
Eukaryotic Initiation Factor-2/metabolism , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Parahippocampal Gyrus/enzymology , Threonine/deficiency , Animal Feed , Animals , Chemoreceptor Cells/enzymology , Immunohistochemistry , Male , Neurons/enzymology , Parahippocampal Gyrus/cytology , Phosphorylation , Rats , Rats, Sprague-Dawley , Signal Transduction/physiology , Statistics, Nonparametric , Threonine/metabolism
12.
Mech Dev ; 121(10): 1289-97, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15327788

ABSTRACT

Cytochrome P450s have generally been acknowledged as broadly tuned detoxifying enzymes. However, emerging evidence argues P450s have an integral role in cell signaling and developmental processes, via their metabolism of retinoic acid, arachidonic acid, steroids, and other cellular ligands. To study the morphogenesis of Drosophila sensory organs, we examined mutants with impaired mechanosensation and discovered one, nompH, encodes the cytochrome P450 CYP303a1. We now report the characterization of nompH, a mutant defective in the function of peripheral chemo- and mechanoreceptor cells, and demonstrate CYP303a1 is essential for the development and structure of external sensory organs which mediate the reception of vital mechanosensory and chemosensory stimuli. Notably this P450 is expressed only in sensory bristles, localizing in the apical region of the socket cell. The wide diversity of the P450 family and the growing number of P450s with developmental phenotypes suggests the exquisite tissue and subcellular specificity of CYP303a1 illustrates an important aspect of P450 function; namely, a strategy to process critical developmental signals in a tissue- and cell-specific manner.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Drosophila Proteins/metabolism , Drosophila/enzymology , Drosophila/growth & development , Sense Organs/enzymology , Sense Organs/growth & development , Amino Acid Sequence , Animals , Animals, Genetically Modified , Chemoreceptor Cells/enzymology , Chemoreceptor Cells/growth & development , Cytochrome P-450 Enzyme System/genetics , Drosophila/genetics , Drosophila Proteins/genetics , Genes, Insect , Mechanoreceptors/enzymology , Mechanoreceptors/growth & development , Mechanotransduction, Cellular , Molecular Sequence Data , Mutation
13.
Neuron ; 42(4): 581-93, 2004 May 27.
Article in English | MEDLINE | ID: mdl-15157420

ABSTRACT

G protein-coupled receptors (GPCRs) mediate diverse signaling processes, including olfaction. G protein-coupled receptor kinases (GRKs) are important regulators of G protein signal transduction that specifically phosphorylate activated GPCRs to terminate signaling. Despite previously described roles for GRKs in GPCR signal downregulation, animals lacking C. elegans G protein-coupled receptor kinase-2 (Ce-grk-2) function are not hypersensitive to odorants. Instead, decreased Ce-grk-2 function in adult sensory neurons profoundly disrupts chemosensation, based on both behavioral analysis and Ca(2+) imaging. Although mammalian arrestin proteins cooperate with GRKs in receptor desensitization, loss of C. elegans arrestin-1 (arr-1) does not disrupt chemosensation. Either overexpression of the C. elegans Galpha subunit odr-3 or loss of eat-16, which encodes a regulator of G protein signaling (RGS) protein, restores chemosensation in Ce-grk-2 mutants. These results demonstrate that loss of GRK function can lead to reduced GPCR signal transduction and suggest an important role for RGS proteins in the regulation of chemosensation.


Subject(s)
Caenorhabditis elegans/enzymology , Chemoreceptor Cells/enzymology , Nervous System/enzymology , Neurons, Afferent/enzymology , Phosphotransferases/metabolism , Receptors, G-Protein-Coupled/metabolism , Animals , Arrestins/deficiency , Arrestins/genetics , Caenorhabditis elegans/cytology , Caenorhabditis elegans/genetics , Caenorhabditis elegans Proteins/genetics , Chemoreceptor Cells/cytology , Cyclic AMP-Dependent Protein Kinases/deficiency , Cyclic AMP-Dependent Protein Kinases/genetics , GTP-Binding Protein Regulators/deficiency , GTP-Binding Protein Regulators/genetics , GTP-Binding Protein alpha Subunits, Gi-Go/deficiency , GTP-Binding Protein alpha Subunits, Gi-Go/genetics , Gene Expression Regulation, Enzymologic/genetics , Mutation/genetics , Nervous System/cytology , Neurons, Afferent/cytology , Phosphoproteins/deficiency , Phosphoproteins/genetics , Phosphotransferases/genetics , Receptors, G-Protein-Coupled/genetics , Signal Transduction/genetics , beta-Adrenergic Receptor Kinases
14.
Comp Biochem Physiol C Toxicol Pharmacol ; 134(1): 139-46, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12524026

ABSTRACT

Nitric oxide (NO), produced by nitric oxide synthase (NOS) in brain tissue, is essential for a variety of kinds of learning in vertebrates. In invertebrates, there are clear examples of an association between NO signalling and olfaction, feeding behaviour and learning. The role of NO as a neurotransmitter in the manipulative behaviour of Sepia officinalis was tested. Manipulative behaviour requires extensive chemotactile sensory processing, fine motor control and probably motor learning processes. NADPH-diaphorase activity (a reliable histochemical marker for nitric oxide synthase) was found in sensory epithelia and in the axial nerve cord of the arms. NOS inhibitor injections (L-NAME) produced an increase in the latency of prey paralysis. By placing mechanical constraints on the base of the fifth periopods of the crab, we prevented the cuttlefish from injecting cephalotoxin and, thus, forced it to change injection sites. We showed that L-NAME pretreatment did not affect the flexibility of the manipulative behaviour. The implications of the involvement of NO in the acquisition of chemo-tactile information and in the programming of the motor skills of the manipulative behaviour is discussed.


Subject(s)
Ganglia, Invertebrate/enzymology , Mollusca/physiology , Nitric Oxide Synthase/antagonists & inhibitors , Predatory Behavior/physiology , Animals , Chemoreceptor Cells/drug effects , Chemoreceptor Cells/enzymology , Chemoreceptor Cells/physiology , Cognition/physiology , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Ganglia, Invertebrate/drug effects , Ganglia, Invertebrate/physiology , NADPH Dehydrogenase/metabolism , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide Synthase Type I , Predatory Behavior/drug effects , Reaction Time/drug effects , Reaction Time/physiology , Sensation/physiology , Sensory Receptor Cells/physiology
15.
Eur J Cell Biol ; 81(10): 539-47, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12437188

ABSTRACT

Chemosensory neurons of the vomeronasal organ (VNO) are supposed to detect pheromones controlling social and reproductive behavior in most terrestrial vertebrates. Recent studies indicate that pheromone signaling in VNO neurons is mediated via phospholipase C (PLC) activation generating the two second messengers inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). Since G alpha(i) and G alpha(o) predominantly expressed in VNO neurons are usually not involved in activating PLC, it was explored if PLC activation may be mediated by G beta gamma subunits. It was found that a scavenger for beta gamma dimers reduced the urine-induced IP3 formation in VNO preparations in a dose-dependent manner indicating a role for G beta gamma complexes. Towards an identification of the relevant G beta and G gamma subunit(s), PCR approaches as well as immunohistochemical experiments were performed. It was found that out of the five known G beta subtypes, only G beta2 was expressed in both G alpha(i) as well as G alpha(o) neurons. Experimental approaches focusing on the spatial expression profile of identified G gamma subtypes revealed that G gamma8-positive neurons are preferentially localized to the basal region of the vomeronasal epithelium, whereas G gamma2-reactive cells are restricted to the apical G alpha(i)-positive layer of the sensory epithelium. As IP3 formation induced upon stimulation with volatile urinary compounds was selectively blocked by G gamma2-specific antibodies whereas second messenger formation elicited upon stimulation with alpha2u globulin was inhibited by antibodies recognizing G gamma8, it is conceivable that PLC activation in the two populations of chemosensory VNO neurons is mediated by different G beta gamma complexes.


Subject(s)
Chemoreceptor Cells/enzymology , Heterotrimeric GTP-Binding Proteins/metabolism , Neurons, Afferent/enzymology , Protein Subunits/metabolism , Type C Phospholipases/metabolism , Vomeronasal Organ/enzymology , Animals , Cell Membrane/drug effects , Cell Membrane/metabolism , Chemoreceptor Cells/cytology , Chemoreceptor Cells/drug effects , Dose-Response Relationship, Drug , Female , Heterotrimeric GTP-Binding Proteins/genetics , Immunohistochemistry , Inositol 1,4,5-Trisphosphate/metabolism , Male , Neurons, Afferent/cytology , Neurons, Afferent/drug effects , Pheromones/metabolism , Protein Subunits/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Odorant/drug effects , Receptors, Odorant/metabolism , Recombinant Fusion Proteins/pharmacology , Second Messenger Systems/drug effects , Second Messenger Systems/physiology , Signal Transduction/drug effects , Signal Transduction/physiology , Vomeronasal Organ/cytology , Vomeronasal Organ/drug effects
16.
Respir Physiol ; 122(2-3): 209-21, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10967345

ABSTRACT

The purpose of this article is to highlight some recent concepts on oxygen sensing mechanisms at the carotid body chemoreceptors. Most available evidence suggests that glomus (type I) cells are the initial site of transduction and they release transmitters in response to hypoxia, which in turn depolarize the nearby afferent nerve ending, leading to an increase in sensory discharge. Two main hypotheses have been advanced to explain the initiation of the transduction process that triggers transmitter release. One hypothesis assumes that a biochemical event associated with a heme protein triggers the transduction cascade. Supporting this idea it has been shown that hypoxia affects mitochondrial cytochromes. In addition, there is a body of evidence implicating non-mitochondrial enzymes such as NADPH oxidases, NO synthases and heme oxygenases located in glomus cells. These proteins could contribute to transduction via generation of reactive oxygen species, nitric oxide and/or carbon monoxide. The other hypothesis suggests that a K(+) channel protein is the oxygen sensor and inhibition of this channel and the ensuing depolarization is the initial event in transduction. Several oxygen sensitive K(+) channels have been identified. However, their roles in initiation of the transduction cascade and/or cell excitability are unclear. In addition, recent studies indicate that molecular oxygen and a variety of neurotransmitters may also modulate Ca(2+) channels. Most importantly, it is possible that the carotid body response to oxygen requires multiple sensors, and they work together to shape the overall sensory response of the carotid body over a wide range of arterial oxygen tensions.


Subject(s)
Carotid Body/physiology , Chemoreceptor Cells/physiology , Hemeproteins/physiology , Ion Channels/physiology , Oxygen/physiology , Animals , Carotid Body/enzymology , Chemoreceptor Cells/enzymology , Humans , Oxygen/blood , Oxygen Consumption/physiology
19.
Arch Biochem Biophys ; 358(2): 204-10, 1998 Oct 15.
Article in English | MEDLINE | ID: mdl-9841634

ABSTRACT

We previously reported that ES20-receptor binding activates phosphoinositide (PI) turnover, resulting in an increase in inositol-1,4,5-trisphosphate, which in turn mobilizes intracellularly stored calcium in the vomeronasal (VN) sensory epithelium of garter snakes. We also found that the activity of adenylate cyclase (AC) in the VN organ is very sensitive to Ca2+ but insensitive to calmodulin regulation. A 250-bp fragment of adenylate cyclase type VI (AC-VI) was obtained from brain cDNA of garter snake by RT-PCR with degenerate primers. The 250-bp fragments were amplified, cloned, and sequenced. Both Northern blot and RNase protection assays revealed that the vomeronasal organ (VNO) and brain contained more abundance of AC type VI than the main olfactory epithelium. A 3.8-kb cDNA was then cloned from the vomeronasal cDNA library of garter snakes and sequenced. The 5' cDNA was obtained by means of 5' RACE PCR and sequenced. We have successfully cloned a 5200-nucleotide cDNA from VNO of garter snakes containing an open reading frame++ encoding 1150 amino acids of AC-VI protein. The vomeronasal AC is termed AC(VN) . AC(VN) shows a high degree of homology with type VI AC of rat, mouse, or human. In situ hybridization with digoxigenin-labeled cRNA demonstrated that AC(VN) mRNA was abundant in the sensory epithelium but not in the nonsensory epithelium of the mushroom body of the vomeronasal organ of garter snakes.


Subject(s)
Adenylyl Cyclases/genetics , Chemoreceptor Cells/enzymology , Cloning, Molecular , Colubridae/genetics , Signal Transduction/genetics , Vomeronasal Organ/enzymology , Adenylyl Cyclases/isolation & purification , Amino Acid Sequence , Animals , Base Sequence , DNA, Complementary/isolation & purification , In Situ Hybridization , Molecular Sequence Data
20.
Brain Res ; 795(1-2): 292-6, 1998 Jun 08.
Article in English | MEDLINE | ID: mdl-9622655

ABSTRACT

The distribution of nitric oxide synthase (NOS) immunoreactive nerve fibers in the carotid body was compared between normoxic and chronically hypoxic rats (10% O2 and 3.0-4.0% CO2 for 3 months). NOS immunoreactive fibers appeared as thin processes with many varicosities. They were distributed predominantly around small arteries and arterioles, and around clusters of glomus cells. When expressed by the density of varicosities per unit area in the parenchyma, the density of NOS fibers associated with the vasculature and with the glomus cells in the chronically hypoxic carotid bodies was significantly decreased. Because nitric oxide (NO) is an inhibitory neuronal messenger in the normoxic carotid body, the present findings suggest that the sensory mechanisms in the hypoxic carotid body may be involved in 'disinhibition' resulting from reduced NO synthesis.


Subject(s)
Carotid Body/enzymology , Hypoxia/metabolism , Nerve Fibers/enzymology , Nitric Oxide Synthase/analysis , Animals , Antibodies , Blood Vessels/enzymology , Carotid Body/blood supply , Chemoreceptor Cells/enzymology , Immunoenzyme Techniques , NADPH Dehydrogenase/analysis , NADPH Dehydrogenase/immunology , Nitric Oxide Synthase/immunology , Rats
SELECTION OF CITATIONS
SEARCH DETAIL
...