ABSTRACT
Introducción: El Injerto de células progenitoras hematopoy éticas (ICPH) es actualmente un tratamiento para diferentes desórdenes hematológicos malignos y no malignos. El análisis del quimerismo post ICPH, y la cuantificación de cada población celular, deben ser monitoreados. El presente trabajo tiene como objetivo: el evaluar los res ultados de quimerismo completo y mixto en sangre periférica del receptor pos trasplante obtenidos por método cualitativo y cuantitativo del año 2000 al 2018. Material y método: El presente es un estudio descriptivo, observacional, transversal de dos mé todos de quimerismo efectuados a receptores y donantes de ICPH alogénico. Resultados: De los 79 pacientes estudiados por el método cualitativo: 65 (82.2%) resultaron con qui merismo completo y 14 (17.7%) con quimerismo mixto. No fue posible cuantificar por este método el % de células del donante y del receptor.Conclusión: El método cuantitativo es un método exacto, que determina el % de células del receptor y del donante presentes en la muestra. Con este método se evalúan un mayor número de marcadores genéticos que con el método cualitativo, y se obtienen un mayor número de loci informativos del quimerismo al compararlo con el método cualitativo.
Introduction: Hematopoietic progenitor cell grafting (ICPH) is currently a treatment for different ma lignant and nonmalignant hematological disorders. The analy sis of postICPH chimerism, and the quantification of each cell population, should be monitored. The present work has as objective: to evaluate the results of complete and mixed chimerism in peripheral blood of the posttrans plant recipient obtained by qualitative and quantitative method from the year 2000 to 2018. Material and method: The present is a descriptive, observational, crosssectional study of two met hods of chimerism performed on allogeneic ICPH recipients and donors . Results: Of the 79 patients studied by the qualitative method: 65 (82.2%) resulted with complete chi merism and 14 (17.7%) with mixed chimerism. It was not possible to quantify by this method the% of donor and recipient cells. Conclusion: The quantitative method is an exact method, which determines the% of recipient and donor cells present in the sample. With this method, a greater number of genetic markers are evaluated than in the qualitative method, and a greater number of information loci of chimerism are obtained than with the qualitative method.
Subject(s)
Humans , Male , Female , Hematopoietic Stem Cell Transplantation/statistics & numerical data , Chimerism/classification , Chimerism/drug effects , Hematologic DiseasesABSTRACT
O quimerismo é uma condição genética rara, que determina que o indivíduo possua dois tipos distintos de DNA em seu corpo. Numa primeira perspectiva, a temática interessaria principalmente aos estudos sobre o DNA e o genoma humano, entretanto o que se verifica é que o fenômeno também tem repercussões jurídicas. Para tanto, serão apresentados alguns casos que demonstram essa relação entre o quimerismo e o Direito (AU)
The chimerism is a rare genetic condition and determines that the individual has two different types of DNA in your body. In a first aspect, the subject matters mainly to the studies of DNA and the human genome, but what is happening is that the phenomenon also has legal repercussions. For this, we present some cases that demonstrate this relationship between chimerism and the law (AU)
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Subject(s)
Humans , Genomics/methods , Chimerism/classification , Genetic Testing/legislation & jurisprudence , Sequence Analysis, DNA/methods , Parthenogenesis/geneticsABSTRACT
OBJECTIVE: Microchimerism has been extensively investigated in autoimmune diseases, which display similarities with graft-vs-host disease. This study was conducted to investigate the presence of microchimerism in minor salivary glands of hematopoietic stem cell transplanted patients, one of the targets of graft-vs-host disease. METHODS: Labial salivary glands biopsy specimens from 11 stem cell transplanted patients were analysed. The samples were grouped in control (five specimens from a female-to-female transplantation) and study group (five glands from male-to-female transplantation). One male transplanted patient was used as a positive control. Fluorescence in situ hybridization with Y-chromosome probe and immunofluorescence with anticytokeratin AE1/AE3 and CD45 were used to identify Y-chromosome positive glandular epithelial cells from allogeneic hematopoietic stem cell transplanted patients. RESULTS: In the study group, all samples were positive to Y-chromosome and cytokeratin AE1/AE3, in agreement with the pattern exhibited by male labial salivary gland. None of the samples from control group were positive to Y-chromosome despite being positive to cytokeratin AE1/AE3. Positivity to CD45 was not relevant. CONCLUSION: Microchimerism in the labial salivary glands of sex-mismatched stem cell transplanted patients is a real phenomenon. Further studies are necessary to elucidate the impact of this phenomenon on the clinical status of stem cell transplanted patients.