ABSTRACT
Three experiments failed to provide consistent evidence for an effect of 2.70 GHz pulsed microwave radiation up to 20 mW/cm2 on pentylenetetrazol-induced seizures or on the efficacy of chlordiazepoxide for counteracting such seizures. Microwave radiation counteracted the hypothermic effects of chlordiazepoxide without altering its antiseizure efficacy. This underscores the dissociation between thermal and pharmacological effects of microwaves.
Subject(s)
Chlordiazepoxide/radiation effects , Microwaves/therapeutic use , Pentylenetetrazole/radiation effects , Seizures/therapy , Animals , Body Temperature/drug effects , Body Temperature/radiation effects , Chlordiazepoxide/therapeutic use , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Male , Pentylenetetrazole/pharmacology , Rats , Rats, Inbred Strains , Seizures/chemically induced , Specific Pathogen-Free Organisms/radiation effects , Time FactorsABSTRACT
It is shown that chlordiazepoxide readily reacts with sodium nitrite in an aqueous HCl solution as well as in human gastric juice to N-nitrosochlordiazepoxide with a high yield (up to 75%). For this reaction the pH optimum has been determined to be 1.4. After oral application of chlordiazepoxide alone or in combination with nitrite to rats, the drug as well as its N-nitroso derivative were recovered from serum and analysed by high-pressure liquid chromatography.
